negation and the temporal structure of narrative discourse

Ngày tải lên: 18/03/2014, 02:20

... of Sciences, the National Academy of Engineering, and the Institute of Medicine. The members of the committee responsible for the report were chosen for their special competences and with regard ... science and technology and to their use for the general welfare. Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the ... services of eminent members of appropriate professions in the examination of policy matters pertaining to the health of the public. The Institute acts under the responsibility given to the National...

Ngày tải lên: 06/03/2014, 15:20

... 4. The influence of the buffer system used for gel electrophor- esis on the band pattern of pale and red Daphnia magna hemoglob- in (Hb). The hemolymph of pale (2.0 lg of Hb) and red (2.0 lgof Hb) ... identified on the basis of their mass spectra, their retention times and characteristic ratio of the a- and b-anomers. Analysis of phosphorylation For the detection of possible phosphorylation, Hb of red D. ... orientation. Accordingly, the present data on the molecular mass of the native Hb complex as well as the Hb subunits are inconsistent. In addition, there are only two hypo- thetical models concerning the structure of...

Ngày tải lên: 16/03/2014, 13:20

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 05/09/2013, 10:17

... building using the program o [42]. Approximately 7% of the randomly chosen reflections were excluded from refinement of the structure and used for the free R-factor calculation [43]. The pro- gram ... Asp291 (2.2 A ˚ ) and the water molecule w1 (2.1 A ˚ ) (Fig. 3A). The DHAP binding site protrudes from the metal N-ter C-ter Fig. 2. Ribbon representation of the overall structure of hACMSD. The (a ⁄ ... present for the last four residues at the C-terminus. The stereochemistry of the model has been assessed using the program procheck [23]. Ninety per cent of the residues fall in the most favoured regions...

Ngày tải lên: 18/02/2014, 06:20

... PAGE. Posi- tions of the top and middle bands coincided well with the two bands observed with the purified enzyme. Thus, it was suggested that the middle and top minor bands of the Ôcomponent BÕ ... integrated and scaled using the programs Mosflm and SCALA in the CCP4 suite [47] with DPS [48]. Structure determination and refinement The structure of the enzyme was determined and refined using the program CNS [49]. ... addition of the other component. Therefore, components A and B were assayed by adding an excessive amount of one component and making the other rate-limiting. PAGE and activity staining of glycerol...

Ngày tải lên: 21/02/2014, 03:20

... on the crystal structures of the human, porcine, and horse PLs (Fig. 1). A frontal view illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the ... tertiary structure. For the two dimeric models of LPL (the closed and open forms, Fig. 1), the active dimeric form of LPL in particular was based on the cocrystallized structures of the complexes of ... to the study of LPL structure and function. LPL shares a high degree of primary-sequence similarity with pancreatic lipase, and the conservation of most of the disulfide bonds between LPL and...

Ngày tải lên: 21/02/2014, 15:20

... factor of 18.8% and an R free of 22.3% with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model is depicted in Fig. 2. The Ca backbones of the ... regularity of the CDase TIM barrel is broken by the a-helices after the sixth b-barrel strand where helix a9 extends in the direction of the preceding strand b14 and only the next helix a10 runs in the ... longer and more complex than the loops at the opposite barrel end. The lengths of the b-strands in the barrel vary from two (b14) to seven (b11) residues. As in other enzymes of family no. 13, the...

Ngày tải lên: 08/03/2014, 02:20

... ambient temperature and analyzed. Results Construction and purification of the engineered proteins of PC The boundary of the BC and CT domains of PC was estimated as follows: the BC subunit of E. coli ... regulator in the interaction between the active site of the BC domain and the biotin moiety of the BCCP domain. Based on these arguments, it is tempting to propose the following hypothesis: in the absence ... upon addition of acetyl- CoA and the activity in the absence of acetyl-CoA was approximately 0.3% of the maximum (Table 1). PC is known to catalyze the cleavage of ATP in the absence of pyruvate...

Ngày tải lên: 16/03/2014, 16:20

... three-dimensional crystal structure of Anx(Gh1) from cotton emphasizes the high conservation of the unique annexin fold even among the members of the plant subfamily of annexin proteins. The fold is comprised of the ... residue in the present structure is s omehow halfway between the loop-in and the loop-out position of the bell pepper annexin. Fig. 1. The three-dimensional structure of Anx(Gh1). (A) The fold of Anx(Gh1) ... n domains II, III and IV are either distorted or the access of a cation to the site is blocked by the p resence of a side chain of a b asic residue. I n case of the IIAB s ite (Fig. 2), the acidic re...

Ngày tải lên: 17/03/2014, 03:20

Ngày tải lên: 17/03/2014, 08:20

Ngày tải lên: 22/03/2014, 23:20

Ngày tải lên: 23/03/2014, 09:20

Ngày tải lên: 31/03/2014, 01:20

Ngày tải lên: 01/11/2013, 14:44

Ngày tải lên: 20/12/2013, 18:26

... drives the United States more than other countries. The second is the uniquely American cult of the deified CEO and his entourage, making them worthy of vast sums of money to attract and retain them. That ... conglomeration of people and their talents. They are systems allowing individuals to interact in the creation of products and services and then selling and deliver- ing them to the marketplace. ... goods and services and sell them in the market. Volcker: That’s right. The purpose of the company is really to provide goods and services at the best possible price, at the highest level of productivity,...

Ngày tải lên: 21/12/2013, 01:19