nanobiosensors for in vitro and in vivo analysis of biomolecules

... acquisition, analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro ... chromatography Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory ... were obtained by venipuncture and separated in aliquots One of them was collected in tubes containing EDTA and assigned to determine haematimetric indexes, plasmatic protein concentration and rheological...

Ngày tải lên: 10/08/2014, 05:21

... technique for specific labelling of recombinant proteins by means of incorporating a radioactive selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core ... embryotoxicity of selenite and selenate: uptake in murine embryonal and fetal tissues and effects on blastocysts and embryonic cells in vitro Toxicology 63, 123–136 3459 In vivo tracking of 75 Se-labelled ... the in ammatory state of the lungs [24] and that these cells play an essential role both in the induction and maintenance of allergen-driven eosinophilic airway in ammation [28–30] Trafficking of...

Ngày tải lên: 07/03/2014, 21:20

... detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate oxygen supply Both changes are accompanied by increasing markers of liver cell injury Future therapeutic interventions ... aim of the present study was to investigate whether the utility of a new and simple remission spectroscopy system allows reliable in vivo detection of liver sinusoidal HbsO2 In a mouse model of ... jugular vein cannula Throughout the experiment, normal saline was administered at a rate of 0.4 ml/hr to maintain normal mean arterial pressure As formerly described [4], and for the realization of...

Ngày tải lên: 13/08/2014, 13:20

... these elements in vitro and in vivo Based on in vivo mutational analyses, two tandem ISL1 binding sites iv are required for enhancer activity in the pituitary and spine and a PITX1 binding site is ... interactions and a central DNA binding homeodomain domain [reviewed in (Hunter and 10 Rhodes, 2005)] ISL1 was first found in the pancreas and was shown to regulate insulin gene expression via the insulin ... alternative splicing of exon Ia and exon Ib The LHX3a and LHX3b protein isoforms are translated from the first methionine of LHX3a and LHX3b mRNAs whereas the M2-LHX3 protein isoform results from...

Ngày tải lên: 24/08/2014, 11:06

... endodomain of envelope proteins, ultimately assisting in viral assembly[11,20] Specifically, NiV matrix (NiV-M) alone, or in combination with its fusion protein (NiV-F) and receptorbinding protein ... diffusing out of the cells), and permits loading of a variety of cell types including primary neuron or microvascular endothelial cells, the main targets of NiV infection Thus, virus-cell fusion of ... through a 20% sucrose (in PBS) cushion followed by resuspension in PBS (Invitrogen) containing 5% sucrose Immunoblotting of VLP proteins βla-M VLP-containing supernatants were lysed and separated by...

Ngày tải lên: 12/08/2014, 04:21

... and cells which participate in bone repair is of utmost importance Bone is composed of around 70-90% of minerals with the rest in the form of proteins Within the proteins in bone, the ratio of ... engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, maintenance and substitution of damaged tissues and organs using principles of ... Release profile of BMP-2 from PCL-TCP scaffolds was plotted for signs of sustained delivery when immersed in PBS solution Heparin was chosen to improve binding and regulate release of BMP-2 here and...

Ngày tải lên: 09/10/2015, 11:18

... submitted for the degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo ... (stereolithography) format into QS (QuickSlice), slicing of the CAD model into horizontal layers and conversion into SLC format, creation of a deposition path for each layer and conversion into SML format for ... NATIVE AND CUSTOMIZED SCAFFOLDS IN VITRO AND THEIR EFFECTS IN INITIAL BONE HEALING 4.1 INTRODUCTION 44 4.1.1 In vitro degradation study 44 4.1.2 In vivo degradation study 45 4.2 MATERIALS AND METHODS...

Ngày tải lên: 09/10/2015, 11:18

... FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound ... (anti-microtubular) agents include the naturally occurring vinca alkaloids (e.g vincristine and vinblastine) and their semi-synthetic analogues (e.g vinorelbine) and the taxanes (e.g paclitaxel and docetaxol) ... characteristics and in vitro performance is studied Moreover, in vivo pharmacokinetic study, complete blood count analysis and toxicity assessment through haematology assay and histological analysis of clearance...

Ngày tải lên: 09/10/2015, 11:18

... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... subdivided into two categories, namely intrinsic and extrinsic factors Intrinsic factors mainly include the genetic make up of the body, which cannot be controlled by the individuals For example,...

Ngày tải lên: 09/10/2015, 11:18

... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... and reversibly to the β- subunit of tubulin in the microtubules rather than to tubulin dimers The binding site to tubulin differs from the one of vinca-alkaloids and podophyllotoxins While vincas ... clearance of drugs once they are present in physiological system is the high probability of binding to endogenous proteins in the circulatory system The high-binding affinity of most commercial formulations...

Ngày tải lên: 09/10/2015, 11:18

... 386 and 387, or at the extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein,...

Ngày tải lên: 07/03/2014, 21:20

... effects of future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, ... MnCl2 in the kinase condition In vitro analysis of NF-jB signaling pathway Tris HCl, pH 7.5, and 10 mm MgCl2 and typically included: 50 ng of rhIKK2; varying concentrations of SC-514 inhibitor ... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the...

Ngày tải lên: 16/03/2014, 11:20

... misfolding of the short Erv1p because FAD binding and in vitro enzyme activity are unchanged Changing C30 or C33 to serine drastically increases the amount of observed dimers Probably, the remaining ... the importance of the N-terminal domain for dimer-formation Under nonreducing conditions all of the cysteine mutant proteins display only a small amount of monomeric form 1532 G Hofhaus et al (Eur ... changes of the protein are associated with the exchange of certain cysteine residues for serine So far, the analysis has revealed the importance of all cysteine residues in the protein, but has...

Ngày tải lên: 17/03/2014, 10:20

... RC & Fofanov Y (2004) Using statistical properties of short subsequences in microbial identification In Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine ... sequences, the strains will be indistinguishable The likelihood of such an occurrence can be reduced by demanding more sequences for distinguishing between any two individual strains; this, however, ... 20% of the 216 22-mers or probe sequences contained in any of their strain members For the 216-probe set, the minimum number of probes differentiating a DENV-1 strain from the all other strains...

Ngày tải lên: 23/03/2014, 11:20

... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing ... corresponding to the insertion stages and b FRF graphs corresponding to the insertion stages and canal and reinserting the prosthesis The FRF had a normal evolution during the reinsertion and the ... der: The influence of contact conditions on resonance frequencies of a hip stemfemur system: a finite element analysis In Proceedings of the International seminar Prediction and evaluation of total...

Ngày tải lên: 20/06/2014, 01:20

... Excellent Vehicle for Site Directed in- vivo Delivery of Drugs and Vaccines Anil Mahapatro1 and Dinesh K Singh2* Bioengineering Program & Department of Industrial and Manufacturing Engineering, Wichita ... writing, editing and revision of this report His lab provided materials and resources used in this study AUTHORS INFORMATION AM: is an assistant professor of Bioengineering in the Department of Industrial ... nanoparticles serve as an excellent vehicle for delivery of a number of biomolecules, drugs, genes and vaccines to the site of interest in- vivo During the 1980’s and 1990’s several drug delivery systems...

Ngày tải lên: 11/08/2014, 08:20

... molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and ... explanation for the discrepancy between the in vitro and in vivo settings might be that the intravenously injected MSCs not reach the spleen and lymph nodes and are therefore unable to inhibit the ... a combination of proliferation and cytokine production, the sera of anti-CD3-injected and MSC-treated mice were analyzed for cytokines The serum of mice was pooled per group and analyzed for the...

Ngày tải lên: 12/08/2014, 11:23

... having an impact on the in vitro behavior of both BoHV-5 strains Concerning the in vivo characterization, in spite of the in vitro differences discussed above, we observed that both BoHV-5 strains ... effects of sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating ... could have some relevance in vivo, for example: i) in vitro total production of infectious virus could be related with in vivo levels of viral excretion, or ii) in vitro cell-to-cell spread could...

Ngày tải lên: 12/08/2014, 18:22

... Yearbook of Intensive Care and Emergency Medicine Heidelberg: Springer-Verlag; 2002 23 Martin C, Papazian L, Perrin G: Performance evaluation of three vaporizing humidifiers and two heat and moisture ... maintain an arterial oxygen tension of at least 80 mmHg Unlike in the in vitro study, the Performer was also evaluated at the three Provider levels (levels I, II and III of heating, or 40, 50 and ... 12 different settings Combinations of two tidal volumes (0.5 and l), two peak inspiratory flow (0.5 and l/s) and three levels of minute controlled ventilation (5, 10 and 15 l/min), with the ventilator...

Ngày tải lên: 12/08/2014, 20:20

... amongst lentiviruses since for HIV-2 and SIV, sequences upstream of the splice donor predominantly contribute to mRNA packaging [9,10] and in FIV regions in U5 and in The ability of one lentiviral ... The dotted line indicates a deletion damaged areas of the brain Such cells have the potential to be useful for the treatment of Parkinson's disease, spinal cord injury and other inflammatory ... immunostaining for GFP, GFAP and β-Tubulin III Immunostaining Lentiviral vector transduced mixed glial cultures were first blocked using 3% goat serum in TXTBS (0.2% triton X100, in Tris Buffered Saline)...

Ngày tải lên: 13/08/2014, 09:21