na ha ratios and tissue culture infectious units tciu

... recombinant pC-HAt, pC -NA and pC-P1 and in vitro transcription and translation The HAt and NA gene of AIV strain H9N2 as well as the recombinant peptide gene P1 was amplified from pY -HA, pY -NA and ... to amplify the NA, HAt and P1genes Primers Sequence pY -NA- Fa pY -NA- R pY-HAt-F pY-HAt-R pH-P1-F pH-P1-R pHybLex/Zeo-F pHybLex/Zeo-R pYESTrp2-F pYESTrp2-R pC -HA- F pC -HA- R pC -NA- F pC -NA- R pC-P1-FP ... both HA and NA proteins although the β-galactosidase activity for HA is nearly fold higher than the NA Although, there was negligible or no interaction between NA and P1 as per the results of NA...

Ngày tải lên: 12/08/2014, 04:21

... ribonuclease A and ribonuclease Tl to digest single-stranded RNA, but not perfectly base-paired double-stranded RNA In this respect RNA:RNA hybrids are more resistant to ribonuclease than RNA:DNA hybrids ... Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 21 Casey, J and Davidson, N (1977) Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations ... of GTC for RNA isolation (IO) It is a simpler procedure that often results in higher yields However, in our hands, we have found the RNA isolated from tissues (but not from tissue culture) sometimes...

Ngày tải lên: 11/04/2014, 09:47

... biết: Rhizoctonia repéns đặc biệt cho Cattleya, Laelie, Cypripedium Rhizoctonia mucoroides cho Vanda Phalaenopsis Rhizoctonia lanuginosa cho Oncidium, Miltonia Nấm cung cấp đường để nuôi cây, phân ... với tỷ lệ cao Hiện nay, phương pháp nuôi cấy mô phương pháp nhân giống lan qui mô công nghiệp Môi trường nuôi cấy: Mỗi loại môi trường nuôi cấy thích hợp cho nhóm lan khác nhau: Knudson C lan ... nhóm lan khác nhau: Knudson C lan Dendrobium, Cattleya MS 1/2 Hyponex (20-20-20) lan đơn thân Vanda, Phalaenopsis, đường bổ sung vào môi trường nuôi cấy 30g/l môi trường làm đặc agar Trước cấy môi...

Ngày tải lên: 04/07/2014, 23:20

... lysoPC (cross-hatched bar), PC (solid bar) and free fatty acids (diagonally hatched bar) B, The distribution of radiolabel between the sn-1 (diagonally hatched bar) and sn-2 (cross-hatched bar) ... original upper phase had reached tube number 10 and all tubes contained both upper and lower phase All 10 complete two-phase systems were each diluted with 10 mM HEPES/KOH pH 7.5, 0.25 M sucrose and ... included in the first tube After shaking and phase separation, the upper phase of the first tube was transferred to a fresh lower phase in tube number two and fresh upper phase was added to the first...

Ngày tải lên: 12/08/2014, 05:20

... range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+ , K+ of case and control groups, with a significant difference in serum + Na of case and control ones ... hypertension and any other systemic disease Then positive ones of both groups were excluded from the study Case and control groups were both examined by an expert ophthalmologist and routine ophthalmic ... an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy and intraocular pressure measurement were done for all...

Ngày tải lên: 03/11/2012, 09:49

... I Chapter Chapter Chapter Chapter Chapter Red Hat Linux System and Network Administration Defined Part II Chapter Chapter Chapter Chapter Chapter Red Hat Linux Network Services 10 Part III Chapter ... Part IV Red Hat Linux System Maintenance Chapter 16 Chapter 17 Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Using the Red Hat Network 403 Upgrading and Customizing ... Sending and receiving e-mail has become so common that it’s hard to remember the time before we had it Chapter 14 explains mail services and its configuration Last, but not least, you find an explanation...

Ngày tải lên: 12/12/2013, 22:15

... kinase incubated with and without ligands (K+, Mg2+ and phosphoenolpyruvate) have been reported previously, and it has been proposed that the distinct spectra correspond to the active and inactive ... energetic of binding of Na+ and K+ to pyruvate kinase It has been hypothesized [18] that the difference between Na+ and K+ in the activation and inhibition of pyruvate kinase could be related to ... pyruvate kinase [32] and 1.45 mLÆmg)1Æcm)1 for lactate dehydrogenase [33] Results and discussion Comparative effect of Na+ and K+ on the activity of pyruvate kinase The effect of Na+ and K+ concentration...

Ngày tải lên: 08/03/2014, 02:20

... (total) ×100% Cell culture The A375 melanoma cell line was purchased from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China) The breast cancer Bcap-37 and colon cancer ... significantly higher than that in the control, 0.03125 mg/mL matrine and 0.625 mg/mL RGD-LCL groups (P

Ngày tải lên: 26/10/2012, 08:57

... the hallmark of the ethanol effect when high concentrations are applied [25] Total RNA extraction and microarray hybridization Total RNA was extracted using RNase kit (Qiagen, Hilden, Germany) and ... than reference, green is less than reference, and gray is missing or excluded data Results Low concentration of ethanol selectively expresses genes involved in transcriptional and translational ... anchoring and signal transduction Additionally, we evaluated the functional pathways in which the ethanol-regulated genes were involved using KEGG (Kyoto Encyclopedia of Genes and Genomes) [36] and...

Ngày tải lên: 31/10/2012, 15:28

... DNA sequencing with chain-terminating inhibitors Proc Natl Acad Sci USA 74: 5463–5467 Tabor S, Richardson CC (1987) DNA sequence analysis with a modified bacteriophage T7 DNA polymerase Proc Natl ... ‘terminators’ into nascent DNA chains (5; Figure 5.3) Basically, a DNA sequencing reaction results in DNA polymerase-directed synthesis of new DNA from a primer annealed to a single-strand template ... experiments and so it is important to ensure that the amplified DNA fragment really represents the DNA sequence of interest This Section covers hybridization analysis, nested PCR and restriction analysis,...

Ngày tải lên: 25/10/2013, 22:20

... what servers you need and how to employ them, and to be aware that it is bad practice and a potential security nightmare to enable services that the system isn’t using and doesn’t need Fortunately, ... Part I Red Hat Linux System and Network Administration Defined CHAPTER Duties of the System Administrator CHAPTER Planning the Network CHAPTER Installing Red Hat Linux CHAPTER Red Hat Linux File ... their passwords periodically, and you can configure Red Hat Linux to prompt users to so And what to about old accounts? Perhaps someone has left the company What happens to his or her account?...

Ngày tải lên: 12/12/2013, 22:15

... Knowledge and Red Hat have assembled these 10 Tips and Tricks from Red Hat Certified Engineers® (RHCEs) to give you an edge on managing these systems 1.Wiping a Hard Drive By Dominic Duval, Red Hat ... process currently has open and mapped to that file descriptor If you have a program that can read input from a file but not from standard input, or that can write to a file but not to standard output, ... each array The name does not matter, as long as it is the same for each array that you want to share the hot spare device(s) Here, we choose "shared" as the name of the spare-group and add an entry...

Ngày tải lên: 21/12/2013, 04:18

... Hat® Linux Tips and Tricks TM Introduction Are you looking for a quick and simple reference guide to help you navigate Red Hat® LinuxTM systems? Look no further Global Knowledge and Red Hat have ... Hat® Enterprise Linux® How I enable logging so that I can see all the queries on my DNS server? If a file has a link count greater than 1, is there a simpler way to find out what other file names ... Tips and Tricks written by Red Hat Certified Engineers® (RHCEs) to give you an edge on managing these systems: If a file has a link count greater than 1, is there a simpler way to find out what...

Ngày tải lên: 21/12/2013, 04:18

... than that of the native 66 kDa ETA and the unmodified N-terminal ETA sequence, suggesting the removal of the C-terminal residues of ETA encompassing the REDLK sequence However, an antibody that ... biochemical FEBS Journal 277 (2010) 3735–3749 ª 2010 The Authors Journal compilation ª 2010 FEBS T El Hage et al and morphological changes (early mitochondrial dysfunction) that are not characteristic ... in vitro cytosol and cell-free mitochondria, our data suggest a direct interaction between ETA and cytosolic EF-2 on the one hand, and the mitochondrial membrane on the other hand However, the...

Ngày tải lên: 18/02/2014, 04:20

... posterior and anterior halves prior to RNA isolation, and induction was assessed in both halves independently bmp2 transcript levels were also measured in both halves because, in the Hamburger–Hamilton ... pre-incubated with ethanol alone (A, lanes and 4; B, lanes and 4) or together with 100 nM at-RA (A, lanes and 5; B, lanes and 5) or 10 lM S-4o9cDH-RA (A, lanes and 6; B, lanes and 6), followed by ... metabolism and function J Neurobiol 66, 606–630 ´ ´ Pares X, Farres J, Kedishvili N & Duester G (2008) Medium- and short-chain dehydrogenase ⁄ reductase gene and protein families: medium-chain and shortchain...

Ngày tải lên: 18/02/2014, 08:20

... functional roles in mammalian tissues Experimental procedures Materials [32P]dCTP[aP] (6000 CiÆmmol)1) and enhanced chemoluminescence (ECL) detection reagents were purchased from Amersham Pharmacia ... pcDNA1.1, pEF1/myc-His and pcDNA 3.1/mycHis/lacZ were purchased from Invitrogen (Carlsbad, CA, USA) Vectors pEGFP-N3 and pDsRed-mito were purchased from BD-Biosciences (Palo Alto, CA, USA) Culture ... glyceraldehye-3-phosphate dehydrogenase (GAPDH) message amplification with GAPDH primers and probe as an internal standard Results were plotted as relative mRNA level vs time (hours) and the slopes...

Ngày tải lên: 19/02/2014, 16:20

... the minus-strand HCV RNA that formed the four base single-strand region and the SL-A1 hairpin As shown in Table 1, our recombinant NS5B was unable to use this RNA as template for RNA synthesis ... previously shown that the recombinant HCV RNA polymerase efficiently replicates the 341 nt of the 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis ... all mutated RNAs and the wild-type (–)IRES RNA for binding to the enzyme NS5B (500 nm) and wildtype [32P](–)IRES RNA (13 nm) were incubated with increasing amounts of cold RNAs and analyzed by...

Ngày tải lên: 20/02/2014, 01:20

... symmetrical and asymmetrical parallel corpora In Proceedings of the 23rd International Conference on Computational Linguistics (COLING’10), Beijing, China Alexandru Ceausu, John Tinsley, Jian Zhang, and ... high input-output ratios for claims, and much lower ratios for abstracts and descriptions, showing that claims exhibit a natural parallelism due to their structure, while abstracts and descriptions ... that have been filed in different countries and are connected through the patent family id We extracted 172,472 patents that were both filed with the USPTO and the EPO and contain an English and...

Ngày tải lên: 22/02/2014, 03:20