na channels and ca2 channels of the cell membrane as targets of neuroprotective substances
Báo cáo khoa học: " Electrolyte Composition of Mink (Mustela vison) Erythrocytes and Active Cation Transporters of the Cell Membrane" ppt
Ngày tải lên :
12/08/2014, 15:20
... (Maede & Inaba 1985) From our present knowledge and in the absence of a Na, K-ATPase and a Na+ gradient the low intracellular concentration of Ca2+ has to be due to a primary Ca2+ pump A Na+ /Ca2+ -exchange ... study the ionic type of red blood cells of the domestic mink is characterized, and moreover, the plasma membrane of mink red cells with respect to the main ion-transporting ATPases: The (Na+ +K+)-activated ... that the intracellular concentration of K+ is very low and apparently lower than the concentration in plasma (see below), whereas the intracellular concentration of Na+ is nearly as high as the...
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Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx
Ngày tải lên :
30/03/2014, 16:20
... guarantee the stoichiometric binding of CaM Regulation of the actin-activated ATPase of recombinant Myo1d proteins by Ca2+ CaM In the absence of free Ca2+ , the basal ATPase activity of the Myo1d-head ... Regulation of Myo1d ATPase activity ATPase activity in Myo1a-c and also the actin-activated ATPase activity of Myo1b The translocation of actin laments by these class I myosins in the gliding assay was ... inhibition of the actin-activated ATPase activity while the basal ATPase activity was unaltered (Fig 6B) The actin-activated ATPase activity increased almost linearly in the range of the actin...
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Accounting skills and programs needed for the next century as viewed by colleges and universities
Ngày tải lên :
30/09/2015, 13:41
... (TCPAS) Established in 1903, the ICPAS is the premier association for CPAs in Illinois The ICPAS is a professional organization of CPAs which, as part o f its activities, assists the students of ... increased expectations of those who entered the profession The current state of most professional accounting education programs was inadequate to meet the needs of the expanded profession The ... knowledge base to meet the professional demands of clients Knowledge skills were classified into the areas of general knowledge, organizational and business knowledge, and accounting and auditing...
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Báo cáo khoa học: Submembraneous microtubule cytoskeleton: biochemical and functional interplay of TRP channels with the cytoskeleton pot
Ngày tải lên :
30/03/2014, 10:20
... remarks The last few years have seen rapid progress in the study of TRP channels as well as other ion channels in the context of both actin and the microtubule cytoskeleton The presence of the microtubule ... for either development of the stereocilia of hair cells in the inner ear or proper localization of TRP channels at the tip of these stereocilia, which is crucial for the activity of these cells ... such as presence of nocodazol or increased Ca2+ concentrations [28] TRPV1 channels are nonselective cation channels Therefore, the role of increased concentration of Ca2+ on the properties of TRPV1–tubulin...
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Báo cáo y học: "dentification of the prokaryotic ligand-gated ion channels and their implications for the mechanisms and origins of animal Cys-loop ion channels" ppt
Ngày tải lên :
14/08/2014, 14:21
... in the type of ligand and the nature of the interactions deposited research One of the major determinants of ion selectivity is the sequence just amino-terminal to the helix M2 on the cytoplasmic ... envelop the ligand, and are the principal constituents of the ligand-binding box (Figure 2) Of these, the dyad of two consecutive cysteines, which are amino-terminal to the final strand of the LBD is ... respect to the mechanism by which the binding of the ligand to a segregated site transmits the conformational change to the rest of the LBDs to trigger the rotation Furthermore, the extent of the applicability...
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ROLE OF VOLTAGE-DEPENDENT K+ AND Ca2+ CHANNELS IN CORONARY ELECTROMECHANICAL COUPLING: EFFECTS OF METABOLIC SYNDROME
Ngày tải lên :
24/08/2014, 11:10
... expression of KV channels contribute to the impaired control of coronary blood flow in the setting of the MetS Aim was designed to delineate the relationship between coronary KV and CaV1.2 channels and ... tone as it has been reported that a change of even mV can yield a 2-fold increase/decrease in [Ca2+ ]i (225; 227) The magnitude of Ca2+ influx through CaV1.2 channels depends on the number of channels, ... has not been fully characterized Other more investigated candidates include components of the reninangiotensin-aldosterone system (RAAS) Notwithstanding the systemic and renal influences of these...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... of DNA content by flow cytometry revealed that the inhibition of cell growth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig 3B) In a total of ... localizes in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of iron metabolism [20,21] After their synthesis in the endoplasmic ... loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II,...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
Ngày tải lên :
06/03/2014, 22:21
... transferrin receptor, which has N-glycosylation sites at residues Asn251, Asn317 and Asn727 Mutation of Asn727 decreases cell- surface expression, whereas mutation at the other two sites does not [42] ... HEK293T cells to express either the wild-type or mutant proteins, and incubated the cells 3222 in the presence or absence of tunicamycin Drug treatments resulted in the accumulation of forms of the ... reticular localization in the same cells (50 ± 2% of N110A-expressing cells, 50 ± 2% of N130A-expressing cells and 58 ± 5% of N234Aexpressing cells, n = 3) This was a feature also of cells expressing...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
Ngày tải lên :
07/03/2014, 16:20
... Comparison of the fasciclin I domains in CLAP with selected proteins in the protein database The Conserved Domain Database of NCBI was screened with the protein coding sequence of clone of CLAP and the ... major bands in e and f, while the numbers at the left are the pI values of standard proteins The arrow at the right represents the pI value of 6.84 calculated for the unmodified CLAP protein based ... substrate and the trinitrobenzene sulfonic acid (TNBS) method [23] One unit of protease activity was defined as the release of micromole of amino peptide per minute from the substrate at pH 4.0 and...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... Effect of VBARP siRNA on cell viability was tested by the trypan blue exclusion assay and cell viability assay The cell viability results of VBARP and control siRNA, compared with the oligofectamine ... routinely used as a measure of apoptosis, in contrast to necrosis Caspase activation occurs at the intersection of all caspase-dependent pathways and is, therefore, an excellent marker of caspase-dependent ... stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of normal cellular events such as cell cycle and...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
Ngày tải lên :
16/03/2014, 16:20
... Glc1Man9GlcNAc2 and peak X were increased (Fig 2A,B) Under these conditions, the decreasing amounts of Man9GlcNAc2 and Man5GlcNAc2 correlated with the increasing amounts of Glc1Man9GlcNAc2 and peak ... look at the end products of processing, most of the Man5GlcNAc2 was converted into Man4GlcNAc2, in contrast with the small amount of Man6GlcNAc2 originating from the Man9GlcNAc2 species ERAD of glycoproteins ... fate of Man5GlcNAc2 bound to glycoproteins in the MI8-5 cell line As described previously for MI8-5 cells, the transfer of Man9GlcNAc2 to glycoproteins led to the formation of Man8GlcNAc2 and...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
Ngày tải lên :
16/03/2014, 18:20
... [2] and was suggested as an explanation for the periodic activation of DNA polymerases in the cell cycle [11] The degree of competition depends on the concentration of free PMLA, DNA, and the ... for the white and the yellow strains Nevertheless, because the level in the cytoplasm of white plasmodia before injection was very low (Table 1), the relative increase in these strains was considerably ... increases the growth rate and shortens the duration of the cell cycle is the polymer-inherent isosterism of the carboxylates with the array of phosphates in nucleic acids and, consequently, the...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
Ngày tải lên :
16/03/2014, 22:20
... Fluorescence signals were calibrated by measuring the fluorescence changes upon addition of known amounts of H2O2 Determination of glutathione The measurement of GSH and GSSG was based on the reaction ... permeabilization of the membrane system Even in the additional presence of 10 mm TEMPOL, significant permeabilization of the mitochondrial membrane occurred, which was shown by the NADH sensitivity of state ... investigation of the impact of single factors and their interplay, such as Ca2+ and ROS Therefore, we exposed isolated rat brain mitochondria to H ⁄ R and ⁄ or Ca2+ and determined H2O2 concentration, membrane...
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Báo cáo Y học: A polymer with a backbone of 3-deoxy-D-glycero -D-galacto -non-2ulopyranosonic acid, a teichuronic acid, and a b-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124 pdf
Ngày tải lên :
17/03/2014, 10:20
... 231.45) as the internal standard, and 80% H3PO4 as the external standard for 31P NMR Presaturation of the HDO signal (1 s) was used in the accumulation of the 1H NMR spectra Two-dimensional spectra ... percentage of the teichoic acid ( 10 % mass of the cell wall) was calculated from the content of the teichoic acidlinked phosphorus (0.8%) and taking into account the structure of the polymer (the ... intensities of the signals in the 1H NMR spectrum It is likely that the percentages of the teichuronic acid, the Kdn-containing polymer, and the ribitol teichoic acid are 30 %, 10 %, and 10 % of the mass...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
Ngày tải lên :
17/03/2014, 23:20
... whether the synthesis of hCdc6p was restricted to specific phases of the cell cycle, HeLa cells were arrested by a double-thymidine procedure at the G1 phase/S phase transition, and then released ... HeLa cells with the proteasome-inhibitor MG-132 prior to the preparation of nuclear extracts In these extracts, the phosphorylated hCdc6p in the control sample was at least as stable as the phosphatase-treated ... chase periods of and h (Fig 5) Total hCdc6p, as determined by Western blotting, was similar in the pulse and in the chase samples (Fig 5A) whereas 35S-labeled hCdc6p decreased during the chase...
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Báo cáo khoa học: A biophysical view of the interplay between mechanical forces and signaling pathways during transendothelial cell migration doc
Ngày tải lên :
22/03/2014, 21:20
... throughout the body of the cell, and the substrate at the basal surface of the ECs Interestingly, forces acting at one surface may be propagated internally or even to other surfaces of the cell, or they ... cascade, and proteolysis leading to apoptosis Activation of the NF-jB pathway leads to recruitment and activation of IjB kinase kinase; the phosphorylation and activation of IjB kinase by IjB kinase ... blood flow and effects from other neighboring cells and matrix The biophysical aspects of the endothelium through which the leukocytes transmigrate, in addition to the biophysical aspects of the leukocytes...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... profiles of MLL1, CGBP, Ash2 and Rbbp5, together with cyclins E and B, as a function of the cell cycle Western blot analysis of the whole -cell extract and histones from different stages of the cell ... at the promoters of specific genes over different phases of the cell cycle 1630 Results and Discussion Dynamics of MLL1 and its interacting proteins during the cell cycle Prior to the analysis of ... al MLL and H3K4 methylations during cell cycle DAPI MLL1 Merge1 Merge2 G1 phase Prophase Metaphase Anaphase Early Telophase Fig Dynamics of MLL1 during the cell cycle Synchronized HeLa cells (at...
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Báo cáo khoa học: A distinct sequence in the adenine nucleotide translocase from Artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on Ca2+ uptake and sequestration pdf
Ngày tải lên :
29/03/2014, 00:20
... 10 passages The homogenate was centrifuged for 10 at 300 g and °C, the upper fatty layer of the supernatant was aspirated, and the remaining supernatant was centrifuged at 11 300 g for 10 The ... case (see Fig 5B); however, the effect of alamethicin in mitochondria treated with ADP was not as great as the effect of the peptide in the absence of the nucleotide, and therefore a reliable comparison ... Ca2+ When cATR or oligomycin was present, the amount of ADP was assumed to be static (see below), and therefore the estimations of free extramitochondrial Ca2+ were reliable In the presence of...
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Báo cáo khoa học: Voltage- and Ca2+-activated potassium channels in Ca2+ store control Ca2+ release docx
Ngày tải lên :
30/03/2014, 10:20
... of E3 chick retina and Fig 1D shows the horizontal plane of the inner layer, where the somata of S-phase cells are located The S-phase cell soma Fig Measurement of the membrane potential of Ca2+ ... accelerate the rate of Ca2+ uptake by Ca2+ pumps, which increase the luminal [Ca2+ ] causing a positive shift in the luminal potential by the electrogenic nature of the Ca2+ pump (phase 4) The luminal Ca2+ ... Nevertheless, the Ca2+ oscillation persisted and the frequency of Ca2+ oscillation was raised at the peak level of the agonist-induced [Ca2+ ]i increase (Fig 3D), where the lower level of Ca2+ ...
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Báo cáo khoa học: Shedding of the amyloid precursor protein-like protein APLP2 by disintegrin-metalloproteinases Retinoic acid-induced upregulation of substrate and proteinase ADAM10 during neuronal cell differentiation ppt
Ngày tải lên :
30/03/2014, 11:20
... to the increase in the amount of APLP2 and not because of the increase in ADAM10 expression But because the BACE-1 mRNA level was increased to a lesser extent, a major role of the nonamyloidogenic ... Roth for excellent technical assistance; R Black for the murine TACE cDNA; C Prinzen for introduction of the HA-tag into the TACE cDNA, and G Thinakaran for providing the APLP2 cDNA and the antibodies ... differentiation of neuroblastoma cell lines on ADAM10 and TACE expression and on the release of secreted APLP2 and APPsa For neuronal (N)-type SH-SY5Y cells, differentiation by RA was accompanied by the...
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