mutations in the tumor suppressor breast cancer susceptibility gene 1 brca1

... occurs in the DNA-binding domain containing the five Kru ¨ ppel-like C 2 H 2 zinc fingers (amino acids 4 01 616 ). O -GlcNAc glycosylation of full-length HIC1 proteins does not affect their DNA binding ... i.e. the zinc fingers domain and the C-terminal end. To confirm these results, the full-length HIC1 protein and two C-truncated HIC1 mutants (1 714 , 1 616 and 1 400; Fig. 4A) were produced in reticulocyte ... proteins did not unravel salient differences in their DNA-binding properties, demonstrating that the glycosy- lation is neither directly nor indirectly involved in the D NA- binding activity. In these...

Ngày tải lên: 30/03/2014, 15:20

... called BRCA1 and BRCA2, and having these genes does make them particularly susceptible to developing breast cancer 11 ,12 . These genes are very rare. Other rare variations in certain genes ... Cancer Inst 99, pp 115 2 -11 61. 27 Katalinic A and Rajal R (2007). Decline in breast cancer incidence after decrease in utilisation of hormone replacement therapy. Breast Cancer Prev Treat (In ... and breast cancer risk. Int J Cancer. , 11 7(4), pp 619 -628. Factors in uencing the risk of breast cancer – established and emerging April 2008 8 Factors in uencing the risk of breast cancer...

Ngày tải lên: 15/03/2014, 01:20

... War1p were raised in rabbits against a GST-War1p fusion protein containing 294 amino acids of the C-terminal part of War1p (amino acids 650– 944) fused in- frame to the C-terminus of GST. The gene fusion ... War1p or a lack of DNA binding. Indeed, in the case of War1-42p, in vivo footprinting data (Fig. 5) indicate an inability to bind to the WARE, which is normally decorated by wild-type War1p in the presence ... unaf- fected, we determined whether War1-42p still binds to the WARE in the PDR12 promoter. To clarify this, we performed in vivo footprinting experiments in cells expressing War1-42p. The strains YPH499...

Ngày tải lên: 19/02/2014, 00:20

... (I) [U] ½ ( M ) p ½ (kbar) DV/n a (mLÆmol )1 ) CM LS WT 4.6 3.0 3 .1 4.65 M88V B 3.2 1. 6 1. 4 19 . 81 T45S I 3.0 1. 8 1. 5 14 .12 D11N S 5.8 >3.2 >3.2 – a The apparent volume change of association (DV) was determined by replotting the ... is contained [11 ,12 ]. The tertiary structure and topology of the MS2 coat protein is different from those of other simple icosahedral viruses [13 ]. The main chain of the protein subunit folds into ... 10 8 10 8 10 2 ND M88V 10 8 10 7 10 2 ND D11N 10 8 10 8 10 7 10 2 Ó FEBS 2003 Cavities and stability in MS2 capsid mutants (Eur. J. Biochem. 2 71) 13 9 High pressure is an efficient tool for studies on the folding...

Ngày tải lên: 19/02/2014, 12:20

... 10 6 ( M )1 Æs )1 ) Wild-type oxidase 1. 4 669 3.7 W121Q 1. 9 11 NR W121G 1. 1 6 NR W121F 1. 5 31 0.05 W121Y 1. 3 22 0.03 Y122Q 2.2 333 0.4 Y122F 1. 2 660 4.6 W121Q/Y122Q 1. 7 9 NR W121Y/Y122W 1. 2 7 0 .13 W121F/Y122F ... positions 12 1 and 12 2 (W121F, W121Y, W121Q/Y122Q, W121F/Y122F, W121G and W121Y/Y122W) were decreased to 36 m M .Ofthe remaining mutants, two showed wild-type behaviour (H 119 N, N160D), whereas all others ... 10 6 ( M )1 Æs )1 ) b Wild-type oxidase 1. 4 669 5.9 10 31 3.7 E142Q 3.2 270 4.7 588 1. 1 D146N 2.7 239 10 .2 357 2.7 E140Q 5.6 277 7.0 250 2 .1 D135N c 2 .1 167 12 .1 104 0.3 D178N c 2.0 213 15 .0 313 2.3 TM1 c 7.9...

Ngày tải lên: 22/02/2014, 07:20

... 2007 The Authors Journal compilation ª 2007 FEBS 893 cm )1 [29]. The intensity increase for the 7 31 (729), 754 (750), 13 06 (13 03), 13 79 (13 75), 14 90 (14 88), 15 84 (15 77), 16 62 (16 68) and 16 95 17 30 ... [29,30] 10 93 vs ÜÜPO 2– sym str [ 41] B-DNA [ 41] , sensitive to electrostatic environment [46] 11 44 w › 11 49 dT [42,45] 11 78 w, as fl 11 86 dT [42,45]; dG [29,43], dC [ 51] 12 13 w, br, sh › 12 18 › 12 18 ... at C ¼ O [ 41, 45] › 16 95 17 20 › 16 99 T C2O str [42,48] at 16 89, shift to 16 81 in case of extra H-bonding at C2 ¼ O [ 41, 45] › 17 30 › 17 11 › 17 36 dG: CO str [44] variable position 16 86 17 22 [44] a Common...

Ngày tải lên: 07/03/2014, 09:20

... caveolae-mediated pathway [24,25]. In this study, the caveolin -1 (CAV1) gene, encoding one of three caveolin proteins, is shown to be a p53-regulated gene. The EGFR and caveolin -1 proteins colocalize in the plasma membrane ... bp Input CAV1 +DO -1 H460 +DO -1 H1299 IR+++ No Ab 10 0 bp M 300 bp 200 bp Input CAV1 +DO -1 H460 +DO -1 H1299 IR–+ + + No Ab 10 0 bp Relativeluciferase activity 0 20 40 60 80 10 0 12 0 –202 to 18 5 ... under confocal settings. The primary antibodies used included EGFR (10 05, sc-03), CD 71 (i.e. TfR, 3B8 2A1, sc-32272), LAMP1 (H5G 11, sc -18 8 21) , caveolin -1 (N-20, sc-894) and HA (Y -11 , sc-805). p53-regulated...

Ngày tải lên: 23/03/2014, 04:21

... located in the a-crystallin domain [10 12 ]. The models built for two mammalian sHsp (aB-crys- tallin [16 ] and HSP27 [18 ]) predict that both these pro- teins contain short a-helices in the N-terminal ... belongs to the group of intrinsically disordered proteins. Accord- ing to the predictions, K137 is located either in the C-terminal part of the b5–b7 loop or in the N-terminal part of the b7 strand, ... containing two a-crystallin domains in the single polypeptide chain [12 ]. Ten different sHsp are encoded in the human gen- ome and are differently expressed in human tissues [13 ,14 ]. None of these...

Ngày tải lên: 23/03/2014, 07:20

... highlight the role of the C-terminal domain in the general folding of the enzyme and, hence, on its activity. This study points to the important role of residues at the IN C-terminal domain in the ... alter the process of DNA integration in vitro. J. Virol. 75, 11 32 11 41. 14 . Brin, E. & Leis, J. (2002a) Changes in the mechanism of DNA integration in vitro induced by base substitutions in the HIV-1U5andU3terminalsequences.J. ... Within the C-terminal domain, deletion of residues 208–286 of ALSV IN or residues 218 –288 of HIV IN proteins result in proteins deficient in multimerization [ 31, 34] and specific mutations in the...

Ngày tải lên: 23/03/2014, 15:21

... become invasive cancer later on. Invasive breast cancer With invasive breast cancer, the abnormal cells have spread beyond the place where they started. Invasive breast cancer can start in the milk ... found in the breast. The cells have not spread to other tissue in the breast or other parts of the body. n LCIS (lobular carcinoma in situ)—With LCIS, abnormal cells grow inside the lobules. In ... women in the United States age 50 to 59. Their risk of getting invasive breast cancer in the next 10 years is about 2 in 10 0. This means that out of every 10 0 women, about 2 will get breast cancer. ...

Ngày tải lên: 29/03/2014, 01:20

... (cm 1 ) 16 51 1552 16 22 15 30 16 08 cm 1 16 22 cm 1 16 35 cm 1 16 93 cm 1 % Variation second derivative % Variation second derivative Temperature (°C) 15 30 15 52 16 22 ΔAbs (a.u.) 16 51 1700 16 50 16 00 15 50 15 00 17 00 16 50 ... present 0.0 0.2 0.4 0.6 0.8 1. 0 –0 .14 –0 .12 –0 .10 –0.08 –0.06 –0.04 –0.02 0.00 0.02 0.04 0.06 0.08 0 .10 0 .12 0 .14 0.0 0.2 0.4 0.6 0.8 1. 0 –0.08 –0.06 –0.04 –0.02 0.00 0.02 0.04 0.06 0.08 0 .10 15 30 cm 1 15 52 cm 1 16 22 cm 1 16 51 cm 1 Temperature (°C) ΔAbs (a.u.) Wavenumber (cm 1 ) Wavenumber (cm 1 ) 16 51 1552 16 22 15 30 ... other S100 proteins, such as S100A12 and S100B, display an increased Ca 2+ affinity on Zn 2+ binding [29,30]. Future investigations might show whether, in these S100 proteins, Zn 2+ increases the...

Ngày tải lên: 30/03/2014, 02:20

... phos- phorylation of Thr172 in the a-subunit, i.e. LKB1 [11 13 ], CAMKKb [15 ,48] and TAK1 [16 ]. LKB1 is believed to be the major upstream kinase for AMPK in skeletal muscle, as knocking out LKB1 almost com- pletely ... is disrupted by disease mutations. J Clin Invest 11 3, 274–284. 21 Kemp BE (2004) Bateman domains and adenosine derivatives form a binding contract. J Clin Invest 11 3, 18 2 18 4. 22 Woods A, Cheung ... Complexes between the LKB1 tumor suppressor, STRAD alpha ⁄ beta and MO25 alpha ⁄ beta are upstream kinases in the AMP-activated protein kinase cascade. J Biol 2, doi :10 .11 86 /14 75-4924-2-28. 13 Woods A,...

Ngày tải lên: 30/03/2014, 08:20

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Ngày tải lên: 18/06/2014, 18:20

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 20/06/2014, 01:20

... A survey of 17 8 NF-Y binding CCAAT boxes. Nucleic Acids Res 26, 11 35 11 43. 12 Mantovani R (19 99) The molecular biology of the CCAAT-binding factor NF-Y. Gene 239, 15 –27. 13 van den Heuvel S & ... complexes of cyclin-dependent kinases (cdks) and their corresponding cyclins. A prominent example of such a pair is cyclin B and cyclin-dependent kinase 1 (cdk1 ⁄ Cdc2) controlling the checkpoint between ... the p107 ⁄ p130-binding domain of Mip130 ⁄ LIN-9 bypasses the requirement for CDK4 activity for the dissociation of Mip130 ⁄ LIN-9 from p107 ⁄ p130-E2F4 complex. Exp Cell Res 315 , 2 914 –2920. 96...

Ngày tải lên: 16/02/2014, 09:20

... October 2007) doi :10 .11 11/ j .17 42-4658.2007.0 616 5.x Retrocyclin RC -10 1, a h-defensin with lectin-like properties, potently inhib- its infection by many HIV -1 subtypes by binding to the heptad repeat ... site of RC -10 1, but a number of RC -10 1 binding conforma- tions. Docking of RC -10 1 to HR1 alone did not result in a strong binding energy [Fig. 2]. Conversely, the minimum energy of binding to HR2 ... against RC -10 1 change the binding site of RC- 10 1 or alter access to the binding site. Both scenarios are possible. In attempting to evade RC -10 1 inhibition, HIV -1 developed three cat ionic mutations, ...

Ngày tải lên: 07/03/2014, 05:20