... perfringens isolates from cattle and pigs with and without Prevalence of C perfringens isolatedfrom fecal samples were shown in Table diarrhea were 85.33%, 86.67%, 82.89%, and 66.67%, respectively ... isolates from diarrheic cattle, 57.34%, 41.33%, and 1.33% belonged to type A, type D, and type C, respectively; whereas all C perfringens isolatedfrom fecal samples of healthy cattle, diarrheic and ... perfringens from healthy and diseased sheep by multiplex PCR Vet Med - Czech 50, 439-442 631 Genotyping of Clostridium perfringens isolatedfrom cattle and pigs with diarrhea in Hanoi and surrounding...
... to compare human tendon/ligaments from different sources, cells from pairs of ACL and PTs isolatedfrom the same subjects and during the same procedure were isolated They were then compared in ... Reagents and consumables Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich (Poole, Dorset U.K.), tissue culture media from Lonza (Wokingham, U.K.) and plasticware from Nunc ... removed and the tendon fascicles were dissected from the surrounding tissues and combined Under sterile conditions tissue samples were rinsed in DMEM, and then diced into small pieces and digested...
... (and suffering) by a physician Today we distinguish euthanasia from natural death (the death the emperor Augustus died) and 12 Euthanasia – The “Good Death” Controversy in HumansandAnimalsfrom ... Controversy in HumansandAnimals distinguishing euthanasia in humansfrom euthanasia in animals; (b) euthanasia is “the act or method of directly causing or allowing the painless and quick death ... sentence of death and life.) - Thomas Aquinas, Summa Theologica, II – IIae, q 64, a 5, ad 14 Euthanasia – The “Good Death” Controversy in HumansandAnimals space and time) and the church punishment...
... dezylana erew etalosi hcae fo AND dimsalp dna stcudorp RCP-per rof snrettap dnaB )aeroK ,reenoiB( tik noitalosi AND dimsalp gnisu siserohportcele leg egoraga dna noitcartxe AND ,sisyl llec ot detcejbus ... relcyc lamreht AND II ONU a htiw demrofrep erew snoitacifilpmA )aeroK ,reenoiB( esaremylop AND fo U dna setahpsohpirt edisoelcunyxoed Mm 52.1 ,remirp hcae fo lomp 02 ,etalosi hcae fo AND lm gniniatnoc ... ta derots dna AND sa desu saw tnatanrepus ehT g × 000,8 ta degufirtnec dna ,nim rof deliob erew yehT retaw dellitsid fo lm 005 ni dednepsus erew seinoloc laudividni 3-2 ,noitalosi AND roF snoitacifidom...
... [15] Since the SP/83/04 (SP83) strain isolated in Thailand in 2004 is LP to ferrets and mice in vivo, and the KAN/353/ 04 (KAN353) strain isolated in Thailand in the same year shows HP to ferrets ... phenotypes associated with the pathogenicity in animals We also used four H5N1 viruses isolated in Thailand in 2004 fromhumans Although a reverse genetics system and animal experiments are needed to confirm ... Materials and methods Viruses and cells Six H5N1 influenza viruses, SP83, KAN353, Thai/1623/ 04 (Thai1623), KK/494/04 (KK494), PCBR/2031/04 (PCBR2031), and SP/528/04 (SP528), isolatedfrom humans...
... isolates from layers, broilers and other domesticated fowl during 1988-97 Acta vet scand vol 44 no 3-4, 2003 Salmonella isolatedfromanimalsand feed production were one S Braenderup and S Typhimurium ... isolated , fromanimalsand feedstuffs in Sweden during 1988-1992 Acta Vet Scand 1995, 36, 21-39 Mårtensson L, Holmberg T, Hurvell B, Rutqvist L, Sandstedt K, Wierup M: Salmonella isolatedfromanimals ... commonly isolated serotype and seven of the isolates (33%) were from broilers and 31 (55%) from layers (Tables 4-8) During the last years, the annual number of isolates from layers, broilers and other...
... typical of group II and group X PLA2s, is absent The predicted molecular mass and pI of the mature protein are 13.5 and 9.1 kDa, respectively This PLA2 clearly differs from the one from A pallida ... I PLA2s This HMM Logo was made from 37 group I, group II and group V PLA2 sequences originating from diverse mammals and snakes In the above HMM model of group I and group II PLA2s, a number of ... PLA2s, and the fact that RNA encoding UcPLA2 was isolatedfrom the sea anemone tentacles, where PLA2s may participate in sting site, irritation, and systemic envenomation syndrome resulting from...
... the isolated EF-hand motif of STIM1 undergoes Ca2+-induced conformational changes and remains as a dimer in the absence and presence of Ca2+ Results and Discussion The isolated EF-hand motif from ... affinity and to probe the oligomeric state of the EF-hand of STIM1 using size-exclusion chromatography and pulsed-field diffusion NMR We found that mutations on loop positions and of the EF-hand from ... FEBS Isolated dimeric EF-hand from STIM1 binds to Ca2+ Y Huang et al Fig Grafting the helix–loop–helix EF-hand motif into CD2 (A) The sequence alignment results of calmodulin EF-hand III and the...
... western blot analysis and well transferred in the membrane fraction Binding properties of muscarinic ligands with M2::GOA-1 fusion protein The binding properties of muscarinic ligands were examined ... aA–aE region and the region upstream of a4 was relatively low, the N-terminal region (aN–a1), the aF–aG region and the C-terminal region (a4–a5) were well conserved between GOA-1 and Gao [31,32] ... receptor recognition are the a2 helix, the b6 ⁄ a5 loop, the a5 helix and the N- and C- extreme termini [31] In addition, the a4 helix and a4 ⁄ b6 loop region of Gai1 are important for specific recognition...
... 1,2-dichloro-4-nitrobenzene (DCNB) and cumene hydroperoxide (CHP), as compared to GSTs isolatedfrom Drosophila melanogaster (DmGSTD1) [22], Nilaparvata lugens (NlGST1-1) [17] and Anopheles gambiae (AgGSTD6) ... information was obtained from LC ⁄ MS ⁄ MS analyses of peptides generated from digestions using trypsin and V8 acid protease Four interpretable mass spectra were obtained from collision-induced ... by Edman degradation and MS are in bold letters and boxes, respectively 1796 FEBS Journal 274 (2007) 1793–1803 ª 2007 Merck and Co., Inc Journal compilation ª 2007 FEBS B Ma and F N Chang named...
... resveratrol [26,27], brassinin [28,29], and deguelin [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from an African plant, Mundule sericea ... and a decreased level of expression of E2F-1 and DP-1 (at 48 h) E2F-1 and DP-1 are known to exist in a complex and act synergistically in E2F site-dependent transcriptional activation [34], and ... (Untxd) SW480 cells at each time point, and protein expression was compared between untreated and treated SW480 cells All bands were compared with b-actin bands using densitometric analysis The...
... epidermidis, and three isolates of S aureus Approximately two-thirds of staphylococci and enterococci isolatedfrom flies were obtained from the homogenized samples (i.e., internalized bacteria), and ... were recovered from four enterococcal isolates from poultry litter and six from fly isolates, all of which also contained the erm(B) gene (Table 4) Two fly isolates from traps and placed in proximity, ... et al (1996) Macovei and Zurek (2006) Macovei and Zurek (2006) Please cite this article as: Graham JP et al., Antibiotic resistant enterococci and staphylococci isolatedfrom flies collected...
... addition, rproBnIb has been found to bind IgE from the sera from allergic individuals Isolation of total RNA and cDNA synthesis Total RNA was isolatedfrom rape seeds (Herborem) as described previously ... PCR-based cloning and sequence analysis The cloning strategy was based on the PCR method using the synthesized cDNA as template and both sense and antisense primers (NIB-1 and NIB-2) and the TaqGold ... antisera raised against Sin a and purified rproBnIb (diluted : 1000 and : 80 000, respectively), a pool of sera from patients allergic to mustard (diluted : 10) and a serum from a patient allergic to...
... corresponding to CMH were isolated as four fractions (Fig 3), each containing galactose and glucose: 66.6% and 33.4% in P-1, 79.6% and 20.4% in P-2, 90.0% and 10.0% in P-3, and 83.5% and 16.5% in P-4 ... contained galactose and glucose: 75.5% and 24.5% in A-1, 68.8% and 31.2% in A-2, and 70.5% and 29.5% in A-3 MALDI-TOF MS spectra (Fig 4) proved that three fractions were CMH, and each of them was ... multilocularis [14,15], and Metroliasthes coturnix [16], and di-, tri- and tetragalactosylceramides from M cortunix [16], T crassiceps [12], and E multilocularis [14,15] Fucosylated di- and tetragalactosylceramides...
... selection distract from the wonders it has produced? Humansand other animals have been endowed with a capacity for genuine love, sympathy, 16 DARWINIAN DILEMMAS and care—a fact that can and will one ... (Frans B M.), Good Natured : the origins of right and wrong in humansand other animals / Frans de Waal p cm Includes bibliographical references and index ISBN 0-674-35660-8 (alk paper) (cloth) ... brand of sociobiology that takes the gene as absolute king Gene-centric sociobiology has managed to reach a wide audience with its message that humansand other animals are entirely selfish From...
... blue and red represent sequences cloned from subjects C98, C18 and D36, respectively E, clones from early viruses; L, clones from late viruses C18E and C98E clones cosegregated separately from ... which was calculated from GeneScan sample files Peaks and bars shown in red represent V1V2 amplimers from early viruses, and peaks and bars shown in blue represent V1V2 amplimers from late viruses ... phenotype and Env sequences of HIV-1 present in SBBC members who were slow progressors or long-term nonprogressors Early and late viruses from D36 were R5X4 whereas viruses isolatedfrom C98 and C18...
... homogeneity from B sphaericus (Misono and Soda 1980) and Brevibacterium sp (Misono et al 1986b), and has been characterized enzymologically The meso-DAPDH genes from C glutamicum (Ishino et al 1988) and ... strains of thermophiles stocked as type cultures andisolatedfrom soils and composts, and found the activity in an aerobically well-grown thermophile from a compost Just recently, meso-DAPDH in a ... Ohshima and Ishida (1992) Finally, the enzyme was extracted from the gel pieces using the standard buffer and Amicon Ultra-15, and the resultant enzyme solution was used for experimentation PAGE and...
... scavenging3,4, and antitumor5,6 activity Various immunoenhancing polysaccharides from edible mushrooms7-9 and hybrid mushrooms10-12 were reported by our group Four different polysaccharides isolatedfrom ... glucan (PS-I) and a heteroglycan (PS-II) consisting of glucose, mannose, and galactose Structural investigation of PS-I showed that it is different from the polysaccharides isolatedfrom either ... All the 1H and 13 C signals (Table 1) were assigned from DQF-COSY, TOCSY, and HSQC experiments The proton coupling constants were measured from DQF-COSY experiment The large JH-2,H-3 and JH-3,H-4...
... scavenging3,4, and antitumor5,6 activity Various immunoenhancing polysaccharides from edible mushrooms7-9 and hybrid mushrooms10-12 were reported by our group Four different polysaccharides isolatedfrom ... glucan (PS-I) and a heteroglycan (PS-II) consisting of glucose, mannose, and galactose Structural investigation of PS-I showed that it is different from the polysaccharides isolatedfrom either ... All the 1H and 13 C signals (Table 1) were assigned from DQF-COSY, TOCSY, and HSQC experiments The proton coupling constants were measured from DQF-COSY experiment The large JH-2,H-3 and JH-3,H-4...