morphology and properties of brain endothelial cells

Báo cáo y học: " GDF-15 is abundantly expressed in plexiform lesions in patients with pulmonary arterial hypertension and affects proliferation and apoptosis of pulmonary endothelial cells" ppsx

Ngày tải lên : 12/08/2014, 13:22

... http://respiratory-research.com/content/12/1/62 Page of 11 Effect of GDF-15 on sprouting of pulmonary endothelial cells To investigate the angiogenic effects of GDF-15 sprouting of human pulmonary microvascu-lar endothelial cells (HPMEC) ... the cells of a plexiform lesion (P) Inset exhibits prominent luminal staining of GDF-15 in cells lining the vascular channel (arrow) Note the presence of GDF-15 in the endothelial cells of neigbouring ... about the functional role of GDF-15 in endothelial cells A previous study demonstrated inhibitory effects of GDF-15 on proliferation, migration and invasion of endothelial cells in vitro as well...

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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Ngày tải lên : 19/06/2014, 22:20

... infection of cerebrovascular endothelial cells Thus, infection of endothelial cells might represent one of the first events in the pathogenesis of TMEV-induced demyelination The persistence of TMEV ... role for endothelial CB2 in the protection and/ or repair of BBB injury However, previous studies of MS brain tissue did not find endothelial expression of CB2 [44,45] In TMEVinfected brain endothelial ... Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors Journal of...

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Báo cáo hóa học: "Fabrication and Properties of Porphyrin Nano- and Microparticles with Novel Morphology" docx

Ngày tải lên : 22/06/2014, 01:20

... dipole of CoTPPS4- and that of H2TAPP4+ in the formation process of porphyrin nanoand micro-particles [33, 34] The Soret and Q bands of sample solution placed for 72 h are not shifted, and the ... is one of the proofs that the pyramids consist of CoTPPS4- and H4TAPP6+ Figure 1c, d shows the images of samples and 3, respectively In Fig 1c, the diameter of the spheres is in the range of 250–1,300 ... porphyrin nano- and micro-particles made up of J- and H-heteroaggregates to date [26] The photocatalytic and photoelectronic properties of TiO2 sensitized with this type of porphyrin nano- and micro-particles...

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Tài liệu Nature and Properties of Micro-organisms doc

Ngày tải lên : 12/12/2013, 17:15

... (VZV) VZV: Chickenpox VZV: Shingles Re-activation of latent infection Suppression of cell mediated immunity Bacteria  Unicellular,  DNA and RNA  Binary  No prokaryotes fission mitochondria ... reducing opsonisation and protecting from phagocytosis  E.g Streptococcus pneumoniae in RTI  vaccination: pneumococcal; meningococcal A and C  GLYCOCALYX loose meshwork of fibrils extending ... cephalosporins and vancomycin lipoteichoic acid, septic shock Beta-lactamases    hydrolyse penicillins and cephalosporins secreted by Gram positive bacteria Within periplasm of Gram negative...

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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

Ngày tải lên : 21/02/2014, 03:20

... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... Construction of pOpacWh Construction of pOpacWh Construction of pOpacSh Construction of pOpacSh Construction of pOpacTh Construction of pOpacTh Construction of pOpacBh Construction of pOpacBh Construction...

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Báo cáo khoa học: Structure analysis of the ﬂavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the ﬂavin reductase family pdf

Ngày tải lên : 07/03/2014, 02:20

... moiety of Arg51 and three atoms of FMN (N1, O2 and O3) In both FeR and M acetivorans avoredoxin, the corresponding residue is asparagine (Asn47 and Asn52, respectively) to which only the O2 atom of ... the Km and kcat values of E coli Fre for riboavin are reported as 2.5 lm and 52.4 s)1 with NADPH as the electron donor and 1.3 lm and 30.6 s)1 with NADH [13] For FMN, Km and kcat values of E coli ... of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant avoredoxin Reduction titration of avoredoxin by NAD(P)H The reduction of...

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Báo cáo khóa học: Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

Ngày tải lên : 07/03/2014, 14:20

... puriﬁcation yield of the enzyme may be due to several reasons Copper and several other divalent ions stimulated the activity of the tetrathionate hydrolase and they and other cofactors may be present ... hydrolase of A caldus (Eur J Biochem 271) 279 The biochemical properties of tetrathionate hydrolase of A caldus were similar to those of other acidithiobacilli (Table 4) The speciﬁc activity of tetrathionate ... method of Lowry et al [27] using the BCA Protein Assay Kit (Pierce, Rockford, IL, USA) The molecular mass and subunit structure of the tetrathionate hydrolase were determined using MALDI-TOF MS and...

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Báo cáo khoa học: Origin and properties of cytoplasmic and mitochondrial isoforms of taurocyamine kinase pptx

Ngày tải lên : 07/03/2014, 21:20

... mitochondrial isoform (referred to henceforth as MiTK) as described below The cDNA for the cytoplasmic TK consists of 1811 bp with an ORF of 1098 bp and 5¢ and 3¢ untranslated regions of 21 and 692 bp, ... other hand, mitochondrial TK showed activity for taurocyamine, lombricine (30% of that of taurocyamine) and glycocyamine (7% of that of taurocyamine) Recombinant Arenicola MiTK was incapable of phosphorylating ... Alignment of mitochondrial targeting sequences of vertebrate and invertebrate MiCKs and Arenicola MiTK Sequences correspond to ubiquitous (uMiCK) and sarcomeric (sMiCK) MiCKs from man and chicken and...

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Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

Ngày tải lên : 08/03/2014, 08:20

... the method of Baayen and Elgersma [13], with a 500-lL drop of Fod conidial suspension at a concentration of · 106 conidiaÆmL)1; 20 additional plants, inoculated with a 500-lL drop of double distilled ... NaPi buffer, pH 3, and acetonitrile (6 : 1, v/v); separation was performed isocratically, at a ﬂow rate of mLÆmin)1, and the volume of injected samples was 10 lL The amounts of the residual initial ... by the addition of two drops of 10 M HCl Aliquots of each reaction mixture were analyzed through column chromatography [7] to separate and purify both the assayed substrates and the respective...

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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

Ngày tải lên : 08/03/2014, 16:20

... lg of platelet protein and 0.5 lg of GST and GST±Rab proteins; (B) an [a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing 40 lg of platelet protein, 0.5 lg of GST and 0.5 lg of ... form of Rab32 (see Discussion) Fig Puri®cation and properties of wild-type and mutant Rab31 and Rab32 expressed as GST±fusion proteins Samples of platelet particulate fraction protein and of puri®ed ... liver and kidney, and was also found in HEL and K562 cells (Fig 3) A 2.0-kb Rab32 mRNA was also detected in some preparations of RNA from HEL cells To demonstrate the presence of Rab31, Rab32 and...

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Morphology and physiology of viruses

Ngày tải lên : 15/03/2014, 12:59

... CLASSIFICATION OF VIRUSES (1) Nucleic acid type: RNA or DNA; single-stranded or doublestranded; strategy of replication (2) Size and morphology, including type of symmetry, number of capsomeres, and presence ... loss of contact inhibition and the piling up of cells into discrete foci Such alterations are a heritable property of the transformed cells Morphologic and Structural Effects Formation of syncytia, ... presence of membranes (3) Presence of specific enzymes, particularly RNA and DNA polymerases, and neuraminidase (4) Susceptibility to physical and chemical agents, especially ether (5) Immunologic properties...

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Temperature dependence of morphology and diameter of silicon nanowires synthesized by laser ablation

Ngày tải lên : 16/03/2014, 15:09

... temperature dependence of the morphology of SiNWs synthesized by laser ablation In this Letter, we present the results on this project Our results show that the morphology and diameter of SiNWs synthesized ... mechanism of the silicon nanoparticle chains They proposed that nucleation and growth occurring alternatively resulted in the formation of chains of silicon nanoparticles The formation of the kinks of ... rate of crystal silicon, outer layer of silicon oxide will surround the crystal silicon As a result, the growth of crystal silicon ceased and silicon oxide of outer layer coalesced together and...

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Mechanics and Properties of Composed Materials and Structures doc

Ngày tải lên : 16/03/2014, 22:20

... The effect of geometrical parameters and material properties of face sheets and core are studied on the face wrinkling of sandwich plates Comparison of the present results with those of plate theories ... anisotropic properties of strength and stiffness of masonry in horizontal and vertical direction, tensile and shear stiffness of cracked masonry, • Concrete yielding in compression, opening of cracks ... tension, mechanism of opening and closing of cracks in concrete under dynamic load, tensile and shear stiffness of cracked concrete, • Strain rate effect of the material properties of masonry, reinforced...

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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf

Ngày tải lên : 17/03/2014, 10:20

... the cloning and expression of the M tuberculosis tps gene in E coli, and the production of active TPS in these cells The properties of the recombinant enzyme have been determined, and are compared ... mammalian cells but it appears to play an important role as a structural component of the M tuberculosis cell wall, and might also function as a stabilizer and protector of membranes and proteins ... M) of NH4HCO3 An aliquot of each fraction was removed and assayed for its radioactive content The radioactive peak was pooled and concentrated to dryness a number of times in the presence of...

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preparation and properties of magnetic iron oxide nanotubes

Ngày tải lên : 20/03/2014, 13:06

... spectra of samples S2 (a) and S3 (b) can be exclusively indexed to ␣-Fe2 O3 , according to standard data (JCPDS 33-0664) In Fig 1(b) and (c), the reﬂection peaks of XRD patterns of S2 and S3, ... in sample S3 (e and f) B Lv et al / Particuology (2008) 334–339 and outer and inner diameters of 70–120 nm and 45–80 nm, respectively Fig 3(c) and (d) show the morphologies of Fe3 O4 nanotubes ... López-Urías, and Mu˜ oz-Sandoval (2005) n simulated the micromagnetic property of iron nanorings, and they found large coercive ﬁelds for din /dout > 0.5 (din and dout are the inner and outer diameters of...

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Báo cáo khoa học: Effect of mutations in the b5–b7 loop on the structure and properties of human small heat shock protein HSP22 (HspB8, H11) pptx

Ngày tải lên : 23/03/2014, 07:20

... the effect of a mutation in this part of the molecule on the structure and properties of human sHsp Oligomeric structure of HSP22 and its mutants All samples of recombinant HSP22 and its mutants ... The N- and C-terminal domains are indicated by N and C correspondingly All b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human ... loop and in the N-terminal part of the b7 strands destabilize the structure of HSP22 Limited trypsinolysis of the wild-type HSP22 and its K137E and K137,141E mutants Fig Far-UV CD spectra of the...

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Báo cáo khoa học: Trehalose synthase of Mycobacterium smegmatis Puriﬁcation, cloning, expression, and properties of the enzyme ppt

Ngày tải lên : 23/03/2014, 13:20

... solution and all run at the same time in the same solvent Standards of trehalose and maltose are applied to the sides of the paper to determine the locations of these sugars, and those areas of the ... 0.5, and 10 mM initial concentrations of maltose (left side) or trehalose (right side) Samples were removed at times up to 22 h Properties of the TreS puriﬁed from M smegmatis Eﬀect of time and ... formation and characterization of the products The conversion of trehalose to maltose was measured by determining the amount of reducing sugar resulting from the production of maltose The amount of...

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Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

Ngày tải lên : 23/03/2014, 15:20

... lower rates of Na+ and K+ pump ﬂuxes result in lower rates of aerobic energy metabolism in turle brains compared with rat brains Superoxide dismutase activities in turtle brain, lung and skeletal ... hydroperoxide as a substrate was not detected in Peak off hydroxylapatite Substrate and inhibitor proﬁles of Peaks and GST isozymes off of hydroxylapatite from aerobic control liver are summarized ... studies characterized the properties of GST in hydroxylapatite Peaks and from control liver Isoelectric focusing Isoelectric focusing of GSTs from liver of aerobic and anoxic turtles is shown...

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Báo cáo Y học: Puriﬁcation and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx

Ngày tải lên : 23/03/2014, 21:20

... toward medium and long chain esters of pNP Maximal reaction temperature is 55 °C and LipA shows strong activity in the presence of metals, inhibitors, and organic solvents Based on these properties, ... (1999) Cloning and sequence analysis of the lipase and lipase chaperone- encoding genes from Acinetobacter calcoaceticus RAG-1, and redeﬁnition of a proteobacterial lipase family and an analogous ... appearance of a zone of clearing Effect of pH on lipase activity and stability Concentrated lipase preparations were diluted fourfold in NaH2PO4-NaOH buffer (50 mM) at various pH values and incubated...

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Báo cáo khoa học: Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells potx

Ngày tải lên : 30/03/2014, 02:20

... overexpression of TPD52 and (B) cell proliferation after downregulation of TPD52 Viability of cells was measured in a colorimetric MTT assay by the detection of formazan formation The proliferation of control ... transfected cells (B) Cells transfected with speciﬁc shRNA after (C) 24 h and (D) 48 h (D) Overlay of (A) and (C) TPD52 downregulation activates caspase activity and increases the loss of mitochondrial ... CARD10 [32] and Vav3 [33], and integrins is important in determining the formation of metastatic cells [34,35] The expression of murine TPD52 in NIH3T3 cells induces the expression of several...

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