molecular mechanism of eukaryotic selenocysteine incorporation

... concentration of the oligomeric species were also decreased Mechanism of neurotoxicity in FAP: the lesson from other amyloidoses A number of studies have examined the mechanism of neurotoxicity ... oligomeric species of TTR The presence of low -molecular- mass TTR aggregates was found to be correlated with the ability of TTR to induce calcium influx via voltage-gated calcium channels High -molecular- mass ... the location of amyloidogenic mutations and position of b-strands (A) The structure of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the N-terminus...

Ngày tải lên: 07/03/2014, 10:20

... effect of RPA on the assembly of the Rad51 presynaptic filament As such, these recombination mediators are critical for the efficiency of HR in vivo We expand on the mechanism of action of the ... without disruption of the filament, and that BRC3 and BRC4 of human BRCA2 interact with different surfaces of Rad51 in the context of the presynaptic filament (122) The sole BRC repeat of the C elegans ... activity of BRCA2 Of special note is the cooperative action of the BRC repeats and CTRB domain in the assembly of the nascent Rad51 filament, with the former acting as the initial depositor of monomeric...

Ngày tải lên: 22/03/2014, 12:20

... representative of PAH, originating from incomplete combustion or pyrolysis of organic matter Under normal conditions, the diet is Benzo[a]pyrene inhibition of lipolysis the main source of B[a]P exposure ... little has been documented on the effect of B[a]P on adipocyte function, one of the main sites of storage We therefore decided to address the question of B[a]P toxicity on adipocytes, and specifically ... Upon withdrawal of this PAH, the excess weight gain was not corrected Our data indicate that inhibition of lipolysis by B[a]P proceeds via direct inhibition of the early step of b-adrenergic...

Ngày tải lên: 30/03/2014, 11:20

... differentiation In conclusion, control of the production of RANKL, of OPG and of other T-cell-derived cytokines in RA patients will provide a clue for strategies of the development of new treatment for inhibiting ... concentration of OPG was detected in the synovial fluids from RA patients The ratio of the concentration of soluble RANKL to that of OPG was significantly higher in the synovial fluids of RA patients ... cell tumor of bone The concentrations of both IL-6 and sIL-6R were significantly higher in the synovial fluids of patients with RA than in those of patients with OA The concentrations of IL-6 and...

Ngày tải lên: 09/08/2014, 06:22

... failure, underscoring the necessity of elucidating the molecular mechanism of PLB action D THE MECHANISM OF Ca2+ TRANSPORT BY SERCA2a SERCA is a large protein of nearly 1000 amino acids that actively ... Inhibition of Cross-Linking of Residues 45-52 of PLB to V89C-SERCA2a 17 Figure 14 Our Model of PLB Regulation of SERCA2a Activity .18 Figure 15 Complete Amino Acid Sequences of the Cross-linkable ... regulated by PLB is of great scientific and clinical interest The overall purpose of this dissertation research was to investigate the molecular mechanism of PLB regulation of SERCA2a Figure Excitation-Contraction...

Ngày tải lên: 24/08/2014, 11:06

... Disclaimer Title of Thesis/Dissertation: The Molecular Mechanism of Break Induced Replication For the degree of Master of Science Choose your degree I certify that in the preparation of this thesis, ...  The Molecular Mechanism of   Break  Induced  Replication  Major  Professor:  Anna  Malkova     DNA  double  strand  break  (DSB)  is  one of  the  most  threatening of  all  types of  DNA ...  understanding  the  underlying molecular  mechanisms of  DNA  repair  processes   is  critical  My  research,  in  trying  to  unravel  the molecular  mechanisms of  BIR  in  yeast,  is   important...

Ngày tải lên: 24/08/2014, 13:24

... MOLCULAR MECHANISM OF SELF-RENEWAL EXIT DURING ENDOTHELIAL DIFFERENTIATION QUE JIANWEN (B Med., China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF SURGERY ... endocytosis of acetylated LDL To understand the molecular mechanism of endothelial differentiation, we began by investigating one aspect of endothelial differentiation i.e inhibition of self-renewal ... differentiation 61 3.1 Study of the molecular mechanism underlying the decisionmaking between self-renewal and differentiation in stem cells 3.2.1 Introduction 3.2.2 Molecular mechanism underlying endothelial...

Ngày tải lên: 16/09/2015, 17:14

... volume (volume of induced cell culture of E coli) of PBS containing 0.1% Triton X-100, 5mM EDTA and protease inhibitor (Roche, Switzerland; every 50ml of lysis buffer requires one tablet of complete ... All spectra represented the average of three scans over the range of 190 to 250nm with a step size of 0.1nm and a band width of 1nm 2.3 T brucei 2.3.1 Culture of procyclic T brucei Procyclic, T ... 2.4.1 Isolation of mRNA from T brucei 37 2.4.2 Synthesis of first-strand cDNA 38 2.4.3 Amplification of ds cDNA by long distance PCR (LD-PCR) 39 2.4.4 Preparation of yeast competent...

Ngày tải lên: 12/10/2015, 17:35

... TABLE OF CONTENTS III SUMMARY V LIST OF TABLES VI LIST OF FIGURES VII LIST OF ABBREVIATIONS VIII CHAPTER1 INTRODUCTION 1.1 Breast cancer 1.1.1 Definition of breast cancer 1.1.2 Incidence of breast ... Removal of only the tumor and a small amount of surrounding tissue Mastectomy Removal of all of the breast tissue Prophylactic mastectomy Preventive removal of the breast to lower the risk of breast ... levels of cyclin D1 occur in a large segment of human cancers Amplification of the cyclin D1 locus accounts for a low proportion of the total number of cancers that overexpresses cyclin D1; the mechanisms...

Ngày tải lên: 12/10/2015, 17:35

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... (Fig 5) Moreover, one of the carboxylate oxygens of Asp153 and the carboxamide oxygen of Asn153 are positioned in such a way as to allow metal coordination interaction with one of the manganese ions ... revealed by the conservation of the whole topology of the active site, would explain the essentially unaltered interaction of the D153N variant with the metallic cofactor In the modelled structure,...

Ngày tải lên: 21/02/2014, 01:21

... effects of 0.1 mgÆmL)1 of apoTf, apolactoferrin, or BSA (as a protein control) on 59Fe mobilization during incubation with increasing concentrations of GSNO (0.025–0.5 mM) However, apoTf, apolactoferrin ... (1992) Biochemistry of nitric oxide and its redox-activated forms Science 258, 1898–1902 Richardson, D.R & Ponka, P (1997) The molecular mechanisms of the metabolism and transport of iron in normal ... Milnes, K (1997) The potential of iron chelators of the pyridoxal isonicotinoyl hydrazone class as effective antiproliferative agents II The mechanism of action of ligands derived from salicylaldehyde...

Ngày tải lên: 08/03/2014, 22:20

... photocleavage [80] of the HC of axonemal dynein Further strong support for a functional role of the Walker motif of AAA1 is provided by molecular dissection of cytoplasmic dyneins in which mutation of the ... the processivity of cytoplasmic dynein [102–104] Molecular dissections of dynactin showed that the mechanism of processivity enhancement is not due to anchoring the motor domain of dynein to microtubules ... tail domain could be one of the mechanisms of regulation of dynein motility [94] In addition, dynein shows large variation in the mode of movements but the mode-switching mechanism is not well understood...

Ngày tải lên: 14/03/2014, 22:20

... type RNases H However, Arg118 of Sto-RNase HI is located in the same position as His124 of Eco-RNase HI, His539 of HIV-1 RNase H and Glu188 of Bha-RNase H Mutation of this residue to Ala considerably ... coordination of the metal ion A, as proposed for Glu188 of Bha-RNase H [31] The catalytic mechanism of RNase H is described in more detail below As described below under ‘Substrate recognition mechanism , ... catalytic mechanism with RNase H Substrate recognition mechanism of RNase H The crystal structure of human RNase H1 in complex with the substrate and Mg2+ highly resembles to that of Eco-RNase...

Ngày tải lên: 30/03/2014, 02:20

... M.sc National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DORCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE Singapore ... efficacy of ABT-869 can not be extrapolated from the experience of related compounds Hence, the clinical applications of ABT-869 will greatly benefit from better understanding of the molecular mechanism ... promising new class of anticancer drugs We demonstrated that combining ABT-869 with SAHA leaded to synergistic killing of AML cells with FLT3 mutations To study the molecular mechanism of their interaction,...

Ngày tải lên: 11/09/2015, 09:06

... Activity of mutants in the conserved patch of scDcp1p 70 Figure 15 Activity of mutants in the conserved patch of scDcp1p 72 Figure 16 Activity of mutants in the hydrophobic surface patch of scDcp1p ... diagram of Dcp1 proteins 23 Figure Schematic diagram of Dcp2 proteins 28 Figure Structures of RNA decapping enzymes 32 Figure Purification of recombinant scDcp1p 54 Figure Purification of recombinant ... years I TABLE OF CONTENT Acknowledgments I Table of content II Summary V Abbreviations VII List of Figures X List of Tables XI Chapter Introduction mRNA turnover 1.1 1.1.1 The life cycle of mRNA 1.1.2...

Ngày tải lên: 14/09/2015, 12:28

... major role in the pathogenesis of both types of diabetes mellitus High levels of free radicals and the decline of antioxidant defense mechanisms lead to damage of cellular organelles and enzymes, ... effects on diabetes, hypertension and wound healing Presumably, one of mechanisms of action is its antioxidant activity The action of EPRT is to produce electrical pressure rather than an electrical ... development of the oxidative stress (68) If our findings are proven by further studies involving a larger number of patients, ultra-low microcurrent therapy might change the concept of management of chronic...

Ngày tải lên: 26/10/2012, 09:39

... Assistant Professor of Medicine and Staff Physician at the Department of Gastroenterology and Hepatology, University Hospital Freiburg, Germany Since 2004 he is the head of the Division of Gastroenterology ... independently of the pre-initiation factors necessary for cap-dependent translation The three-dimensional structure of the HCV IRES bound to the 40S ribosomal subunit illustrates the molecular basis of ... the genome from nucleic acid binding proteins Recent advances in the understanding of the molecular mechanisms of this enzyme could enable a specific inhibition as a novel antiviral strategy [47,...

Ngày tải lên: 02/11/2012, 10:00

... necessary should the rate of emergence of resistance and the impact of such mutations turn out to be clinically relevant for therapy management Molecular Mechanisms of Drug Resistance HBV is ... is the appearance of serum markers of HBV infection that establishes the diagnosis of the disease Of these, HBsAg is considered to be the sentinel marker for the confirmation of acute infection ... production [46,47] Clinical Impact of Hepatitis B Genotypes For a DNA virus, the HBV genome shows an exceptional degree of molecular variation (incorporation of some 1010 mistaken nucleotides...

Ngày tải lên: 03/11/2012, 09:41

Ngày tải lên: 07/06/2013, 01:26

... shows the contribution of this strategy to the development of plants with biofarming potential in the frame of a high demand of natural and ecologically accepted sources of renewable compounds ... the evolution of vascular plants The case of Fld and Cyt c6 shows that reintroduction of these genes in the proper subcellular compartment of model and crop plants restored some of the selective ... export of the excess of reducing power via the malate valve, and productive consumption of the surplus of NADPH by the Calvin cycle Finally, as is the case with iron deficiency, the extent of protection...

Ngày tải lên: 25/10/2013, 05:20