molecular imaging of rna interference therapy targeting phd2 for treatment of myocardial ischemia
Noninvasive Imaging of Myocardial Ischemia docx
Ngày tải lên :
28/06/2014, 20:20
... or a combination of both can cause myocardial ischemia Noninvasive Imaging of Myocardial Ischemia Table 1.1 Important factors in the control of myocardial oxygen supply and myocardial perfusion ... Foreword II Noninvasive Imaging of Myocardial Ischemia provides a comprehensive discussion and review of the noninvasive myocardial imaging techniques that are currently available to detect myocardial ... assessment of LV function becomes important for the assessment of the precise site and extent of jeopardized myocardium Noninvasive Imaging of Myocardial Ischemia Table 2.2 Indications for echocardiography...
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MOLECULAR IMAGING OF THE LUNGS docx
Ngày tải lên :
29/06/2014, 11:20
... SPECT as Platforms for Molecular Imaging 59 Buck E Rogers I Introduction 60 II SPECT Imaging 60 III PET Imaging 64 IV SPECT Imaging vs PET Imaging 67 V Applications of PET and ... density of targets for antisense imaging are low (just two DNA strands per cell and up to 105 mRNA molecules per cell for some highly expressed genes) Therefore, imaging of DNA and mRNA transcripts ... some classes of radiopharmaceuticals developed for molecular imaging Although only a limited number of these have been used to date for pulmonary imaging, many are suitable for studies of lung cancer,...
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báo cáo khoa học: " Molecular imaging of potential bone metastasis from differentiated thyroid cancer: a case report" docx
Ngày tải lên :
10/08/2014, 23:20
... patients with thyroid cancer for correct characterization of equivocal tracer uptake seen on planar imaging, as well as for precise localization of Sandu et al Journal of Medical Case Reports 2011, ... informed consent was obtained from the patient for publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of ... Sandu et al.: Molecular imaging of potential bone metastasis from differentiated thyroid cancer: a case report Journal of Medical Case Reports 2011 5:522 Author details Department of Neurological...
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Báo cáo y học: " Molecular imaging of transcriptional regulation during inflammation" docx
Ngày tải lên :
11/08/2014, 03:20
... [4] Based on such findings the development of NF-κB modulators for treatment of inflammatory diseases has been given a lot of attention As imaging of transcription factor activity is basically ... and Carlsen Journal of Inflammation 2010, 7:20 http://www.journal-inflammation.com/content/7/1/20 Page of 11 Table 1: Overview of transgenic reporter mice available for studies of inflammation ... the spatial patterns of activation Kielland and Carlsen Journal of Inflammation 2010, 7:20 http://www.journal-inflammation.com/content/7/1/20 Page of 11 Table 2: Overview of imaging studies related...
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Báo cáo y học: "Molecular imaging of transcriptional regulation during inflammationƠ" docx
Ngày tải lên :
11/08/2014, 06:22
... [4] Based on such findings the development of NF-κB modulators for treatment of inflammatory diseases has been given a lot of attention As imaging of transcription factor activity is basically ... and Carlsen Journal of Inflammation 2010, 7:20 http://www.journal-inflammation.com/content/7/1/20 Page of 11 Table 1: Overview of transgenic reporter mice available for studies of inflammation ... the spatial patterns of activation Kielland and Carlsen Journal of Inflammation 2010, 7:20 http://www.journal-inflammation.com/content/7/1/20 Page of 11 Table 2: Overview of imaging studies related...
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Báo cáo y học: "Atorvastatin pretreatment diminishes the levels of myocardial ischemia markers early after CABG operation: an observational study" doc
Ngày tải lên :
10/08/2014, 09:22
... study group of the present study Cardiac isoforms of troponin are specific myocardial injury markers indicating the level of perioperative myocardial ischemia Moderate elevations of troponin ... Sciascio G: Randomized trial of atorvastatin for reduction of myocardial damage during coronary intervention: results from the ARMYDA (Atorvastatin for Reduction of MYocardial Damage during Angioplasty) ... before and at 24 hours after the operation If serial blood samples had been obtained instead of a single measurement after the operation, the course of myocardial ischemia under atorvastatin treatment...
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Báo cáo y học: " Statin pretreatment diminishes the levels of myocardial ischemia markers not only in CAB" ppsx
Ngày tải lên :
10/08/2014, 09:23
... effects and the capacity of reduction of myocardial biomarkers, are the reasons of the potencial beneficial effects of statins in cardiac surgery interventions List of abbreviations CABG: Coronary ... this article as: Martínez-Comendador et al.: Statin pretreatment diminishes the levels of myocardial ischemia markers not only in CABG Journal of Cardiothoracic Surgery 2010 5:131 Submit your next ... December 2010 References Ege E, Dereli Y, Kurban S, Sarigul A: Atorvastatin pretreatment diminishes the levels of myocardial ischemia markers early after CABG operation: an observational study J Cardiothorac...
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Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx
Ngày tải lên :
23/03/2014, 07:20
... 539 bp, respectively Preparation of GIH-dsRNA Production of GIH-dsRNA by in vitro transcription Two DNA templates for dsRNA of GIH that span the coding sequence of the mature Pem-GIH, each containing ... plasmid of hairpin -RNA of Pem-GIH was subsequently transformed into an RNaseIII-deficient E coli HT115 strain Expression of hairpin -RNA was induced with 0.4 mm isopropyl thio-b-d-galactoside for h ... using the specific primers for the 5¢-region of Pem-GIH The negative PCR is indicated by )ve The actin transcript of P monodon was used as an internal control of the amount of RNA template Each panel...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
Ngày tải lên :
23/03/2014, 10:20
... ability of dsRNA to inhibit CHH function in shrimps in vivo So far, there is little information concerning the use of RNA interference (RNAi) in crustacean species Recently it has been proved that RNA ... 5671 Functional analysis of CHH by RNAi Glucose (mg/dL) 20 16 J M Lugo et al 0h 24 h 12 Saline Unrelated dsRNA CHH dsRNA Fig Effects of injection of dsRNA in the profile of the hemolymph glucose ... of the CHH transcript could be achieved in 24 h Numerous factors could influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of...
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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
Ngày tải lên :
21/06/2014, 02:20
... pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers EJNMMI Research 2011 1:1 Submit your manuscript to a journal and benefit from: ... responses to therapy (i.e., estimating HER1 expression) and would provide accurate data for performing dosimetric calculations for radioimmunotherapy [36] The caveat to translating the imaging of HER1 ... permitting repeated treatment of patients [20] The ability to administer multiple doses of mAb for either therapy or imaging has not been a trivial consideration in the management of cancer patients...
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Báo cáo y học: "Autoimmune targeting of key components of RNA interference" doc
Ngày tải lên :
09/08/2014, 08:22
... responsible for the post-transcriptional regulation of gene expression [10,12] RNAi is triggered by double-stranded RNA (dsRNA) precursors that are rapidly processed into small RNA duplexes of approximately ... molecular mass forms of the purified complex [10] These larger forms are thought to be due to the weak and/or transient association of proteins involved in the initial processing of dsRNA (for example, ... in length by a dsRNA-specific endonuclease termed Dicer [10] These small RNA duplexes commonly referred to as short interfering RNAs (siRNAs) or miRNAs incorporate into the RNA- induced silencing...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
Ngày tải lên :
16/02/2014, 09:20
... small interfering RNA (siRNA)-based depletion of IAP increases apoptosis in response to gefitinib, IAPs might be a molecular target for the induction of apoptosis by TKIs Inhibition of cell proliferation ... Furthermore, p38a, one of the four p38 isoforms, is required for Bax activation and apoptosis Because activation of p38 by UVB irradiation in human keratinocytes results in induction of a conformational ... and the level of protection correlates with the extent of Bim reduction, indicating that Bim is essential for gefitinib-induced apoptosis of NSCLC cells The induction of Bim after treatment with...
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Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx
Ngày tải lên :
07/03/2014, 11:20
... efficiency for the downstream ORF in unprocessed pri-miRNAs However, expression of the marker (regardless of the placement location) could only occur at the expense of failure of cleavage of primiRNA ... Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference Nucleic Acids Res 32, 936–948 FEBS Journal 273 (2006) 5421–5427 ª 2006 The Authors Journal ... vectors generate efficient knockdown of target proteins To examine the efficiency of generation of RNAi for pSM155 and pSM30, we examined the knockdown of expression of firefly Luciferase (Luc) HeLa cells...
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Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt
Ngày tải lên :
16/03/2014, 05:20
... types of RNAi, with slight differences in the mechanism They are mediated by either: (a) dsRNA; or (b) small interference (si )RNA The longer dsRNA may generate a large population of siRNA (with ... solution, lL of UTP solution, and lL of T7 Enzyme Mix The mixture was incubated at 37 °C for 18 h During the transcription, the two RNA strands were hybridized to form dsRNA For RNAi experiments, ... and the use of longer dsRNA may be advantageous over siRNA In this study, the longer dsRNA was produced and used in the RNAi experiments It has been reported that the longer dsRNA of approximately...
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Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf
Ngày tải lên :
18/06/2014, 16:20
... activity) RNA Interference Assay Small interfering RNAs (siRNA) for interferon regulatory factor IRF-3, IRF-7, virus-induced signaling adapter (VISA), and the non -targeting control (NC) siRNA were ... denaturation at 96°C for 10 min, cycling conditions were 96°C for 15s, 60°C for for 40 cycles The viral DNA in each sample was quantified by comparing the fluorescence profiles with a set of Ad DNA standards ... responsiveness of breast cancer to chemotherapy and radiation therapy based on the expression pattern of ISGs In this study, we identified by transcriptional profiling two ISG-defined phenotypes of pancreatic...
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Báo cáo hóa học: " Adenoviruses with an avb integrin targeting moiety in the fiber shaft or the HI-loop increase tumor specificity without compromising antitumor efficacy in magnetic resonance imaging of colorectal cancer metastases" pdf
Ngày tải lên :
18/06/2014, 16:20
... resonance imaging (MRI) Tumors are marked with arrows Picture of liver metastasis of mock treated (PBS) animal (A) day before treatment and (B) on day 35 after treatment (C) Picture of liver metastasis ... Barcelona, Spain) for the cloning and large-scale production of most of the viruses used for this article Especially, we thank Prof Alemany for his advice during the development of this work We ... domain mutated to GATK for HSPG ablation AdTLGR -RGD insertion in HI loop of fiber knob for avb integrin targeting AdTLYG -Y477A substitution in DE loop of fiber knob for CAR ablation -Fiber...
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Báo cáo hóa học: "Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference" pdf
Ngày tải lên :
18/06/2014, 16:20
... blinded to the treatment group Preparation of plasmid based ADAM 10 shRNA vector The ADAM 10 small interfering RNA (siRNA) sequence (CAGUGUGCAUUCAAGUCAA) was designed using the software siRNA Target ... (Promega, Madison, WI, USA) The preparation of the RNAi vector expressing the human ADAM 10 short hairpin RNA (shRNA) was performed using the pSuper siRNA expression plasmid with the U6 promoter ... transfection of ADAM 10 siRNA resulted in a significant reduction of cellular growth of adenoid cystic carcinoma cells Our data are in line with previous reports showing that Xu et al Journal of Translational...
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Molecular Imaging: An Essential Tool in Preclinical Research, Diagnostic Imaging, and Therapy potx
Ngày tải lên :
29/06/2014, 11:20
... consequence of the convergence of molecular and cell biology with imaging science, including medical physics and technology One of the major goals of molecular imaging has become the development of noninvasive ... tissues, e.g., b-galactosidase for molecular biology and thymidine kinase for molecular imaging However, molecular imaging is still far from the achievements of molecular biology that started ... Ribozyme Antigene Tweezer Sense Aptamer SiRNA, lRNA Mechanism of activity Base pairing Molecular recognition RNA + ± RNA DNA RNA DNA /RNA Binding Protein Any RNA Ô + + ± ± ± ± + ± + + ± Double helix...
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Báo cáo sinh học: "Functional genomics of cell morphology using RNA interference: pick your style, broad or deep" doc
Ngày tải lên :
06/08/2014, 18:20
... with fixed cells Depletion of any of eight single kinesins compromised formation of a bipolar spindle or the alignment of chromosomes on the metaphase plate, but depletion of multiple kinesins with ... set up the ‘Drosophila RNAi Screening Center’ (DRSC) [12], with a library of RNAi reagents for all of the known open reading frames in the Drosophila genome and facilities for high-throughout screens ... chain (unnamed open reading frame CG15780) In four of these cases, one of the labs did not test the RNA, but with depletion of the remaining 11 RNAs one lab found a lamellar defect whereas the...
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Báo cáo sinh học: "A functional genomic analysis of cell morphology using RNA interference" pptx
Ngày tải lên :
06/08/2014, 18:20
... generation of gene-specific dsRNAs Screens of RNAi morphological phenotypes required the generation of a dsRNA library In order to allow an assessment of the overall success of such an RNAi screening ... CG10522 in the RNAi screen as a potential novel Rho1-effector required for cytokinesis [21] RNAi targeting of members of a different group of genes resulted in a profound loss of actin filaments ... dsRNA to Rho1, a gene required for cytokinesis [10], to optimize conditions for RNAi in a 384-well plate format The addition of 0.3 g Rho1 dsRNA to cells for a minimum of days in culture generated...
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