molecular and functional characterization of p glycoprotein in vitro

... WRKY-encoding genes in transcriptional reprogramming during P pachyrhizi infection in soybean plants [17-22] To determine which soybean WRKY genes are involved in plant defense against P pachyrhizi infection, ... genotype (PI970230), which carries the Rpp2 gene, and in the soybean susceptible genotype (Embrapa 48) in response to P pachyrhizi infection were compared with that of uninfected leaves (mock inoculation) ... families of regulatory proteins in plants Previous studies have identified 72 WRKY-encoding genes in Arabidopsis [24], approximately 100 members in rice [25-28], 104 in poplar [29], 86 in Brachypodium

Ngày tải lên: 27/05/2020, 01:13

... using agar plates containing 100 μg/mL kanamycin, followed by nucleotide sequencing of both strands to confirm in- frame insertion 2.6 Expression and Purification of Recombinant CcPrx4 Protein in ... contained a 28 bp 5-untranslated region (UTR), a single open reading frame (ORF) of 741 bp encoding a peptide of 247 amino acids and a 115 bp 3-UTR, including a stop codon (TAA) and a poly ... blue and beta-turns in grey Balls in purple and copper represent CYS97 and CYS218, respectively 3.2 Sequence Alignment and Phylogenetic Analysis of the CcPrx4 Protein As shown in Figure 2, the predicted

Ngày tải lên: 04/12/2022, 10:31

... to investigate expression pattern of each Gcdsx variant, we performed expression analyses via PCR We dissected heads of last instar, early and late prepupal period, and early and late pupal period ... sequences in G cornutus via RAPD-PCR.  The sex of G cornutus is indistinguishable by external morphology during larval and prepupal periods, so we developed PCR-dependent sexing methods before the molecular ... important to know the sex of sample individuals in advance However, some insects not show morphological dimorphism, especially in larval and prepupal periods, which are critical periods in investigating

Ngày tải lên: 04/12/2022, 15:39

... neural plate bending with the hinge point model Blue represents dorsolateral hinge points and red represents median hinge point Asterisks indicate furrowing... over-simplified and described ... to Professor Bay Boon Huat for facilitating the transfer by introducing and recommending me to my supervisor and to Professor Ling Eng Ang for giving me the chance to join the Department of Anatomy ... the brain Bending of the neural plate is based on the 7 Chapter 1: Introduction formation of three hinge points in the neural tissue: a median hinge point (MHP) where the neural plate

Ngày tải lên: 11/09/2015, 09:04

... Design and production of Der p alanine mutants with single amino acid substitution 127 6.3 Reaction profile of dust mite allergic patients from Singapore and Italy 133 6.4 IgE epitope mapping of ... liposomes 107 5.3 Binding of Der p to purified lipids 109 5.4 Analysis of lipid extracts from nDer p and rDer p 112 5.5 Characterization of potential cholesterol binding sites in Der p via site directed ... (1988) IgE production by normal human lymphocytes is induced by interleukin and suppressed by interferons gamma and alpha and prostaglandin E2 Proc Natl Acad Sci U S A 85(18): 6880-6884 Piboonpocanun

Ngày tải lên: 15/09/2015, 17:09

... synthase NTC no template control ODQ 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one ONOO peroxynitrite OP open probability PAG DL-propargylglycine PDE phosphodiesterase PE phenylephrine PGE1 prostaglandin ... mononucleotide GDP guanosine diphosphate GPCR G protein-coupled receptor GTP guanosine triphosphate H2S hydrogen sulphide HRP horseradish peroxidase HSP heat shock-related protein hVSMCs human ... serine/threonine kinase), which would then phosphorylate the myosin binding subunit (MBS) of MLCP. Phosphorylated MLCP is the inactive form of MLCP and therefore, it will promote higher levels of

Ngày tải lên: 12/10/2015, 17:33

... diphosphate, DMAPP undergoes condensation with one or more IPP in head-to-tail fashion to produce linear diphosphates such as geranyl diphosphate (C10, GPP), farnesyl diphosphate (C15, FPP) and ... quantified in six different Neem tissues including kernel, pericarp, flower, leaf, stem and bark using UPLC-ESI(+)-HRMS based targeted profiling Tissue specific profiling of triterpenoids delineated ... contain 22 and 45 mg/g of triterpenoids (including chlorophyll and other pigments), while stem and bark furnished 15 and 10 mg/g of the tissue respectively Standard graphs were prepared for each of

Ngày tải lên: 26/05/2020, 22:00

... cruzi, arginine can be phosphorylated by arginine kinase, producing phosphoarginine, a specific phosphagen and high-energy phosphate compound [17, 18] involved in cell energy storage and in pH and ... Hepes, and 60 mM Pipes, pH 7.3; PPase: Vacuolar-type proton-pumping pyrophosphatase; TAU: Triatomine artificial urine; TAU3AAG: Supplemented with amino acids and glucose; TCDB: Transport Classification ... an arginine analog found primarily in the seeds of certain leguminous plants It displays cytostatic and anti-proliferative effects in T cruzi epimastigote cultures, incorporating into proteins,

Ngày tải lên: 02/11/2022, 11:34

... numerous DNA replication proteins: including T antigen and DNA polymerase a-primase; the tumor suppressor p5 3; the transcription factors Gal4 and VP16; the DNA repair proteins XPA, DDB, XPF, XPG, uracil ... Overexpression of recombinant OsRPA70a and OsRPA70b The coding regions of OsRPA70a and OsRPA70b were cloned into the pET24a (OsRPA70a) and pET28a (OsRPA70b) expression vectors (Novagen) For protein expression, ... not shown) In vitro pull-down assays were performed using the following protein pairs: T7 tag-OsRPA70a and OsRPA32-His tag (Fig 3C), OsRPA70b-His tag and 3273 Two types of RPA in seed plants T

Ngày tải lên: 07/03/2014, 21:20

... of the protein fractions in the calcareous base and periphery, indicated that Mrcp-19k was present in the primary cement and protein fraction in the calcareous base, but not in the peripheral ... as the signal peptide that has been cleaved off in the mature pro- tein. The amino acid sequences of the N-terminal and three internal peptide fragments of Mrcp-19k deter- mined experimentally ... caused loss of the protein in solution. Inspection of the chemical forms of two Cys residues in the recombinant protein was performed as follows. The protein was digested with lysylendopeptidase

Ngày tải lên: 16/03/2014, 05:20

... (B,C) Actin polymers in the presence of Iba1 (B) and Iba2 (C). (D,E) For comparison, actin polymers are shown in the presence of the actin cross- linking protein neurabin-2 (D) and an actin binding ... respectively (Fig. 3). The presence of Ca 2+ had only a marginal effect on the dimerization of both Iba proteins. F-actin binding and cross-linking Iba1 is known to bind and cross-link actin polymers [12]. ... outgoing helix and the conformational change of EF-hand proteins upon Ca 2+ binding [18]. Thus, it may be concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca 2+ binding. Function

Ngày tải lên: 16/03/2014, 06:20

... especially in the mammalian forebrain, throughout the lifespan of the animal This amino acid binds to the Gly-binding site of the N-methyl-d-aspartate subtype of the Glu recep- tor in the brain, and potentiates ... the functions of neuroendocrine and endocrine tissues d-Asp suppresses melatonin release in the pineal gland [9,10], stimulates prolactin secretion in the anterior pituitary gland [11,12], modulates ... to the depth of understanding of d-amino acids in mammalian physiology, the importance of d-amino acids in the biological function of higher plants remains unknown To investigate the physiological

Ngày tải lên: 30/03/2014, 04:20

... CD3-allophycocyanin (APC)-Cy7, CD14-APC-Cy7, CD56biotin and CD16-APC-Cy7, streptavidin-APC-Cy7, CD10-PE; CD24 fluorescein isothiocyanate (FITC), CD20-PE, CD27PE, CD27-FITC, CD80-FITC; CD86-PE from ... distinctive feature of chronic inflammatory arthritides is the presence of synovial lymphocytic infiltrates that play a role in disease pathogenesis through the release of pro-inflammatory cytokines ... DRB1*0801, DPB1*0201), and the presence of antinuclear antibodies In the majority of these patients, the disease remains confined to a limited number of joints (persistent oligoarticular JIA) and has

Ngày tải lên: 09/08/2014, 14:22

... (KO) lines of RAP2.4 and RAP2.4L Top left, expression levels of RAP2.4 and RAP2.4L in the single knockout lines of RAP2.4 (RK) and RAP2.4L (RLK) determined by RT-PCR Top right, expression ... group members and three A-6 subgroup proteins) bind the CE1 element in yeast. Several of the CEBFs have been reported as GCC box binding proteins. For example, the preferred in vitro binding ... of RAP2.4 and RAP2.4L in the double knockout line (DK) Bottom, plants grown in soil... sequences of AtERF13, RAP2.4 and RAP2.4L were prepared and cloned into pBI101.2 [22] Page 11 of

Ngày tải lên: 11/08/2014, 11:21

... A B Fig Expression of VBARP isoforms in vitro and in vivo: (A) In vitro transcription ⁄ translation of VBARP-L and VBARP-S One microgram of VBARP-L, VBARP-S and vector plasmid was in vitro transcribed ... role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or ... transcribed to cDNA and PCR amplified using One Step RT-PCR Superscript II (Invitrogen) and primers specific for VBARP and b-actin (housekeeping gene) molecules The following VBARP primers were designed:...

Ngày tải lên: 07/03/2014, 21:20

... Corticotropin-releasing factor type and type alpha receptors regulate phosphorylation of calcium/cyclic adenosine 3¢,5¢-monophosphate response element-binding protein and activation of p4 2 /p4 4 mitogen-activated ... Perkins, A.V., Europe-Finner, G., Lowenstein, P. R & Linton, E.A (1996) Corticotrophin-releasing hormone receptor type 1: generation and characterization of polyclonal antipeptide antibodies and ... with HindIII and EcoRI and inserted in the vector described above This plasmid was named pLuc The minimal promoter containing TATA box was amplified from pcDNA6/V5-HisA vector (Invitrogen) by primers...

Ngày tải lên: 07/03/2014, 15:20

... the intracellular domain of Lrp5/6 contains Axin2 binding sites (Mao et al., 2001) Thus, binding of Axin might be the trigger for inactivation of the phosphorylation of β-catenin The binding ... SmaI and SalI and ligated into the digested target vector 2.3.9.3 TOPO/pDrive cloning of riboprobe templates Purified PCR products of partial lrp5 and crestin transcripts were ligated into the pDrive ... Lrp5 and Sost are conserved at the sequence level Fig 11 Early embryonic expression of lrp5 and sost Fig 12 lrp5 and sost expression at 24 and 48 hpf Fig 13 72 hpf and dpf expression of lrp5 and...

Ngày tải lên: 10/09/2015, 08:43

... helicase ⁄ NTPase domain are indicated in gray and black respectively (B) Purification of the recombinant Rep245 protein SDS ⁄ PAGE of protein extracts at various stages of the purification of Rep245 Lane ... have counterparts in Pfuprimase, although not in the pRN1 prim–pol protein In archaeal and eukaryotic primases, the K135 residue (the counterpart of R148 in Pfu-primase) is part of a highly conserved ... Supporting information The following supporting information is available: Fig S1 The in uence of (A) divalent cations, (B) pH and (C) temperature on Rep245 polymerase activity This supporting information...

Ngày tải lên: 18/02/2014, 18:20

... analysis of the purified hCYP11B1 (A) The purified hCYP11B1 was applied on RP-HPLC analysis using POROS R1 ⁄ 10 (2.1 · 100 mm; Applied Biosystems) as described in Experimental procedures The protein ... from h11B1 and that at 15.5 contained the apoprotein The apoprotein peak was collected and subjected to MALDI-TOF MS to verify the integrity of the protein moiety on Voyager DE-Pro (Applied Biosystems) ... volume and energetic properties of the binding of CO to hemoproteins Biophys J 66, 89–98 Tuckey RC & Kamin H (1983) Kinetics of O2 and CO Binding to adrenal cytochrome P- 450scc Effect of cholesterol,...

Ngày tải lên: 30/03/2014, 04:20

... Elzinga M (1987) Phosphorylation of the 20,000-dalton light chain of smooth muscle myosin by the calcium-activated, phospholipiddependent protein kinase Phosphorylation sites and effects of phosphorylation ... C2-type Ig (Ig-C2) superfamily The Ig-C2 domains are found extensively in adhesion molecules and in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin ... (1993) Molecular cloning of chicken myosin-binding protein (MyBP) H (86-kDa protein) reveals extensive homology with MyBP-C (C-protein) with conserved immunoglobulin C2 and fibronectin type III...

Ngày tải lên: 30/03/2014, 04:20