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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf

báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf

Ngày tải lên : 20/06/2014, 04:20
... Central Page 1 of 9 (page number not for citation purposes) Virology Journal Open Access Methodology A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open ... This strategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon 1 for genotype 1b. To increase the sensitivity of amplifica- tion for these regions, ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype 1a and 1b patient plasma samples averaged over 95% for all amplicons....
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

Ngày tải lên : 18/02/2014, 13:20
... than required for thioflavin T (ThT)-based fibrillation assays (typical concentrations used are 3–10 lm), but is not sufficient for other biophysical studies. We therefore examined a number of methods ... applied to a carbon-coated formvar grid, left for 1 min, fixed with glu- taraldehye, wicked dry with filter paper, and 2% uranyl acetate was added and the mixture was incubated for 2 min. The grid was ... filtrate from fractions eluted at 150–200 mm NaCl. For both Ab(M1–40) and Ab(M1–42), all manipulations were performed at slightly alkaline pH to avoid the formation of structural contaminants pro- duced...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Ngày tải lên : 18/02/2014, 17:20
... lm. A new method for mouse endothelial cell culture T. Yamaguchi et al. 1990 FEBS Journal 275 (2008) 1988–1998 ª 2008 The Authors Journal compilation ª 2008 FEBS Development of a new method for isolation ... University of Tokyo, Japan) for providing pCAG-Cre; Dr Ikuo Yana (Institute of Medical Science, University of Tokyo, Japan) for help in per- forming endothelial tube formation experiments. This work ... Tokyo, Japan). For immunocytochemistry, cells were fixed on ice with 4% PFA in NaCl ⁄ P i for 10 min, incubated in methanol at )20 °C for 20 min, and rehydrated in NaCl ⁄ P i . For detec- tion...
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Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf

Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf

Ngày tải lên : 20/02/2014, 04:20
... of the Association for Computational Linguistics, pages 180–189, Portland, Oregon, June 19-24, 2011. c 2011 Association for Computational Linguistics A New Dataset and Method for Automatically ... dataset for training and test- ing such systems and comparing their performance. As it is likely that the deployment of such systems will increase, standardised and independent evalua- tion methods ... style, grammar, and so forth, though the methods used to assess these are not described in any detail in published work. Again, the system requires retraining or tuning for new prompts and assessment...
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Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf

Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf

Ngày tải lên : 20/02/2014, 12:20
... 100. For future work, integrating SEPA into the rerank- ing process seems a promising direction for enhanc- ing overall parser performance. Acknowledgement. We would like to thank Dan Roth for ... normalizing over sentence lengths is less of a factor. For this reason, in this paper we present detailed graphs for the aver- age f-score. For completeness, Table 4 also provides some of the results ... al., 2006). For completeness of reference, Table 4 shows the superiority of SEPA over CB in terms of the usual f- score measure used by the parsing community (num- bers are counted for constituents...
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Tài liệu Báo cáo khoa học: "A Tool for Multi-Word Expression Extraction in Modern Greek Using Syntactic Parsing" pdf

Tài liệu Báo cáo khoa học: "A Tool for Multi-Word Expression Extraction in Modern Greek Using Syntactic Parsing" pdf

Ngày tải lên : 22/02/2014, 02:20
... (Seretan, 2008) which has previously been 1 For the sake of simplicity, we will henceforth use the term Greek to refer to Modern Greek. used to build MWE resources for other languages, including English, ... rules defined for Greek, allowing for the complete parse of about 50% of the sentences in a corpus like Europarl (Koehn, 2005), which contains proceedings of the European Parliament. For the remaining ... 45–48, Athens, Greece, 3 April 2009. c 2009 Association for Computational Linguistics A Tool for Multi-Word Expression Extraction in Modern Greek Using Syntactic Parsing Athina Michou University...
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Spring Data Modern Data Access for Enterprise Java pdf

Spring Data Modern Data Access for Enterprise Java pdf

Ngày tải lên : 22/03/2014, 09:20
... like Example 2-13. It contains methods to save a single entity as well as an Iterable of entities, finder methods for a single entity or all entities, and delete(…) methods of different flavors. Example ... and scientific laboratories had been performing this process before the term big data came into fashion. What makes the current process different from before is that the value derived from the ... anyway) for properties and parse the method name into a property traversal tree. To avoid these kinds of typos as early as possible, STS’s Spring Data support offers code completion for property...
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Novel method for sputum induction using the Lung Flute in patients with suspected pulmonary tuberculosis pdf

Novel method for sputum induction using the Lung Flute in patients with suspected pulmonary tuberculosis pdf

Ngày tải lên : 29/03/2014, 03:20
... Flute for sputum sampling in patients suspected of pulmonary tuberculosis (TB). Methods: Thirty-four patients who were not expecto- rating sputum, but for whom sputum examination was required for ... Capilia TB assay-positive. ‡ See text for explanation of further examinations for the diagnosis of TB. AFB, acid-fast bacillus; TB, tuberculosis. Novel method for sputum induction in TB 901 © 2009 ... has yet to be cleared for use in patients by the Japanese authority. Individual Lung Flutes were supplied for each patient by Medical Acoustics, Tokyo, Japan. Written informed consent was obtained...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx

Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx

Ngày tải lên : 19/06/2014, 08:20
... Central Page 1 of 9 (page number not for citation purposes) Virology Journal Open Access Methodology A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open ... This strategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon 1 for genotype 1b. To increase the sensitivity of amplifica- tion for these regions, ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype 1a and 1b patient plasma samples averaged over 95% for all amplicons....
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Book Manual Getting Started Guide for Peachtree Complete Accounting_13 pdf

Book Manual Getting Started Guide for Peachtree Complete Accounting_13 pdf

Ngày tải lên : 20/06/2014, 18:20
... payroll reports, 941 forms, W-2 forms, and W-3 forms. For more information, see “ Assign W-2 Payroll Fields in Peachtree Accounting” on page 266. Assign Payroll Fields for EmployEE- and EmployER-Paid ... use department masking. For more information, refer to the “General Ledger” chapter of the Peachtree Accounting User’s Guide. Budget Information Budget information for each QuickBooks account ... Peachtree Accounting as stock items using the costing method selected during the conversion process. For more information about costing methods, refer to the “Inventory” chapter of the Peachtree...
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Book Manual Getting Started Guide for Peachtree Complete Accounting_14 pdf

Book Manual Getting Started Guide for Peachtree Complete Accounting_14 pdf

Ngày tải lên : 20/06/2014, 18:20
... Information Adding or Editing Company Information Company/Company Info Maintain Company Information window Converting QuickBooks Data to Peachtree Accounting Getting Started Guide 278 B For ... number. Then select Post or Save to record the transaction. For more information on entering payments, see Pay Vendors. For more information on entering receipts, see Receive a Payment from a ... select Default Information from the Maintain menu. Then, select Employees. The Employee Defaults window displays. For detailed information on what and how QuickBooks job information is converted...
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Báo cáo hóa học: " A stabilized mixed discontinuous Galerkin method for the incompressible miscible displacement problem" pdf

Báo cáo hóa học: " A stabilized mixed discontinuous Galerkin method for the incompressible miscible displacement problem" pdf

Ngày tải lên : 20/06/2014, 22:20
... stabilized mixed DG method for the velocity and pressure 4.1 Elimination for the flux variable u Letting a(c)=a(c) -1 . For the velocity and pressure, we define the following forms a ( u, v; c ) = ( α ( c ) u, ... (D l−2 (T h )) d × D l−1 (T h ) , (6:18) where (6.17) clearly holds for every θ Î (0,1) for the case ((6.4 ), (6.8)), and for every θ < 0 for the case ((6.10), (6.13)). On the other hand, since ∇ h D l-1 (T h ) ... mixed finite element (MFE) method for the compressible miscible displacement problem. For the Darcy flow, Masud and Hughes [2] introduced a stabilized finite element formulation in which an appropriately...
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Báo cáo hóa học: " Research Article A New Method for Least-Squares and Minimax Group-Delay Error Design of Allpass Variable Fractional-Delay Digital Filters" pdf

Báo cáo hóa học: " Research Article A New Method for Least-Squares and Minimax Group-Delay Error Design of Allpass Variable Fractional-Delay Digital Filters" pdf

Ngày tải lên : 21/06/2014, 07:20
... will lead to the same solution. The formal formulation for LS group-delay error design of allpass VFD filters will be presented in Section 3,in which an iterative method is proposed to replace the ... complexity, although the design time of the proposed method is much more than the existing methods, an alternative method has been revealed in this paper for further research in the future. References [1] ... denominator in (22) is ignored for the iterative method in (23), which will yield satisfactory results. Equation (23) can be further represented in a quadratic form as e k ( a k ) = s τ + a T k Q s a k +...
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