... than required for thioflavin T (ThT)-based fibrillation assays (typical concentrations used are 3–10 lm), but is not sufficient for other biophysical studies. We therefore examined a number of methods ... applied to a carbon-coated formvar grid, left for 1 min, fixed with glu- taraldehye, wicked dry with filter paper, and 2% uranyl acetate was added and the mixture was incubated for 2 min. The grid was ... filtrate from fractions eluted at 150–200 mm NaCl. For both Ab(M1–40) and Ab(M1–42), all manipulations were performed at slightly alkaline pH to avoid the formation of structural contaminants pro- duced...
Ngày tải lên: 18/02/2014, 13:20
... lm. A new method for mouse endothelial cell culture T. Yamaguchi et al. 1990 FEBS Journal 275 (2008) 1988–1998 ª 2008 The Authors Journal compilation ª 2008 FEBS Development of a new method for isolation ... University of Tokyo, Japan) for providing pCAG-Cre; Dr Ikuo Yana (Institute of Medical Science, University of Tokyo, Japan) for help in per- forming endothelial tube formation experiments. This work ... Tokyo, Japan). For immunocytochemistry, cells were fixed on ice with 4% PFA in NaCl ⁄ P i for 10 min, incubated in methanol at )20 °C for 20 min, and rehydrated in NaCl ⁄ P i . For detec- tion...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf
... of the Association for Computational Linguistics, pages 180–189, Portland, Oregon, June 19-24, 2011. c 2011 Association for Computational Linguistics A New Dataset and Method for Automatically ... dataset for training and test- ing such systems and comparing their performance. As it is likely that the deployment of such systems will increase, standardised and independent evalua- tion methods ... style, grammar, and so forth, though the methods used to assess these are not described in any detail in published work. Again, the system requires retraining or tuning for new prompts and assessment...
Ngày tải lên: 20/02/2014, 04:20
Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf
... 100. For future work, integrating SEPA into the rerank- ing process seems a promising direction for enhanc- ing overall parser performance. Acknowledgement. We would like to thank Dan Roth for ... input for systems that require high quality parses, what matters most is that SEPA outperforms the MR baseline at the high k ranges. Figure 4 (bottom) shows the same pattern of re- sults for the ... Linguistics, pages 408–415, Prague, Czech Republic, June 2007. c 2007 Association for Computational Linguistics An Ensemble Method for Selection of High Quality Parses Roi Reichart ICNC Hebrew University...
Ngày tải lên: 20/02/2014, 12:20
APPENDIX A TO PART 136 METHODS FOR ORGANIC CHEMICAL ANALYSIS OF MUNICIPAL AND INDUSTRIAL WASTEWATER: METHOD 605—BENZIDINES pdf
... Practices for Sampling Water,” American Society for Testing and Materials, Philadelphia. 9. “Methods 330.4 (Titrimetric, DPD-FAS) and 330.5 (Spectrophotometric, DPD) for Chlorine Total Residual,” Methods ... established performance criteria to determine if the results of analyses meet the performance characteristics of the method. When results of sample spikes indicate atypical method performance, a ... using the extract produced by this method. 1.3 The method detection limit (MDL, defined in Section 14.1) for each parameter is listed 1 in Table 1. The MDL for a specific wastewater may differ...
Ngày tải lên: 14/03/2014, 20:20
Novel method for sputum induction using the Lung Flute in patients with suspected pulmonary tuberculosis pdf
... Flute for sputum sampling in patients suspected of pulmonary tuberculosis (TB). Methods: Thirty-four patients who were not expecto- rating sputum, but for whom sputum examination was required for ... Capilia TB assay-positive. ‡ See text for explanation of further examinations for the diagnosis of TB. AFB, acid-fast bacillus; TB, tuberculosis. Novel method for sputum induction in TB 901 © 2009 ... has yet to be cleared for use in patients by the Japanese authority. Individual Lung Flutes were supplied for each patient by Medical Acoustics, Tokyo, Japan. Written informed consent was obtained...
Ngày tải lên: 29/03/2014, 03:20
báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... This strategy was effective for amplifying all amplicons except for amplicon 4 for both genotype 1a and 1b and amplicon 1 for genotype 1b. To increase the sensitivity of amplifica- tion for these regions, ... percentage. BioMed Central Page 1 of 9 (page number not for citation purposes) Virology Journal Open Access Methodology A general method for nested RT-PCR amplification and sequencing the complete ... of sufficient DNA for sequencing with any one of the primer pairs for a given amplicon) for amplification of 72 genotype 1a and 1b patient plasma samples averaged over 95% for all amplicons....
Ngày tải lên: 20/06/2014, 04:20
Báo cáo hóa học: " A stabilized mixed discontinuous Galerkin method for the incompressible miscible displacement problem" pdf
... stabilized mixed DG method for the velocity and pressure 4.1 Elimination for the flux variable u Letting a(c)=a(c) -1 . For the velocity and pressure, we define the following forms a ( u, v; c ) = ( α ( c ) u, ... (D l−2 (T h )) d × D l−1 (T h ) , (6:18) where (6.17) clearly holds for every θ Î (0,1) for the case ((6.4 ), (6.8)), and for every θ < 0 for the case ((6.10), (6.13)). On the other hand, since ∇ h D l-1 (T h ) ... mixed finite element (MFE) method for the compressible miscible displacement problem. For the Darcy flow, Masud and Hughes [2] introduced a stabilized finite element formulation in which an appropriately...
Ngày tải lên: 20/06/2014, 22:20
Báo cáo hóa học: " Research Article A New Method for Least-Squares and Minimax Group-Delay Error Design of Allpass Variable Fractional-Delay Digital Filters" pdf
... will lead to the same solution. The formal formulation for LS group-delay error design of allpass VFD filters will be presented in Section 3,in which an iterative method is proposed to replace the ... complexity, although the design time of the proposed method is much more than the existing methods, an alternative method has been revealed in this paper for further research in the future. References [1] ... denominator in (22) is ignored for the iterative method in (23), which will yield satisfactory results. Equation (23) can be further represented in a quadratic form as e k ( a k ) = s τ + a T k Q s a k +...
Ngày tải lên: 21/06/2014, 07:20
báo cáo hóa học:" Research Article Uniform Second-Order Difference Method for a Singularly Perturbed Three-Point Boundary Value Problem" pdf
... h i1 /2 be an average mesh size. Before describing our numerical method, we introduce some notation for the mesh functions. Define the following finite differences for any mesh function v i vx i ... for an ordinary d ifferential equation,” Computational Mathematics and Mathematical Physics, vol. 35, no. 11, pp. 1417–1422, 1995. 8 G. F. Sun and M. Stynes, “A uniformly convergent method for ... 2010, Article ID 102484, 13 pages doi:10.1155/2010/102484 Research Article Uniform Second-Order Difference Method for a Singularly Perturbed Three-Point Boundary Value Problem Musa C¸ akır Department...
Ngày tải lên: 21/06/2014, 11:20
Báo cáo hóa học: " Research Article A Novel Method for Improving Fairness over Multiaccess Channels" pdf
... heterogeneity such that for Δ (I) > 15 dB, SIC is clearly superior to OMA. The beneficial effects of the newly proposed strategy can be observed for BORG ∗ K , which performs as well as OMA for small Δ (I) , ... not use formula (7.31) from [3] because it was derived for SIC with residual errors propagated from previous steps. It is clear that, for T ∈{2, , K − 1}, we do not need to compute AME for all ... in which the groups are decoded. Remark for T = 1 that the grouping method is the same with OMA. Moreover, the g rouping method is identical with SIC for T = K. Similarly to conventional SIC,...
Ngày tải lên: 21/06/2014, 11:20
Báo cáo hóa học: "Research Article A Computationally Efficient Method for Polyphonic Pitch Estimation" pdf
Ngày tải lên: 21/06/2014, 19:20
Báo cáo hóa học: " A Method for Single-Stimulus Quality Assessment of Segmented Video" pdf
Ngày tải lên: 22/06/2014, 23:20
Báo cáo hóa học: " A Practical Radiosity Method for Predicting Transmission Loss in Urban Environments" pdf
Ngày tải lên: 23/06/2014, 00:20
Báo cáo hóa học: " A New Method for Estimating the Number of Harmonic Components in Noise with Application in High Resolution Radar" pdf
Ngày tải lên: 23/06/2014, 01:20
Everyone Is a Customer - A Proven Method for Measuring the Value of Every Relationship in the Era of Collaborative Business pdf
Ngày tải lên: 28/06/2014, 22:20
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