methylation chromatin and transcription
Báo cáo Y học: When the embryonic genome flexes its muscles Chromatin and myogenic transcription regulation ppt
Ngày tải lên :
31/03/2014, 21:21
... the local chromatin infrastructure through recruitment of HAT- and possibly HDAC-enzymes in the course of myogenesis CHROMATIN REMODELING IN MUSCLE DIFFERENTIATION Chromatin structure and nucleosomal ... for HMG1 and linker histones B4 and H1 in organizing chromatin EMBO J 15, 548–561 21 Ura, K., Nightingale, K & Wolffe, A.P (1996) Differential association of HMG1 and linker histones B4 and H1 with ... MyoD (and probably Myf5) recruit mammalian SWI/SNF complexes to initiate target gene transcription However, formal proof of a physical interaction between MyoD and Brm/Brg-1 containing chromatin...
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Báo cáo y học: "Chromatin and siRNA pathways cooperate to maintain DNA methylation of small transposable elements in Arabidopsis" ppsx
Ngày tải lên :
14/08/2014, 15:20
... field, and we have shown that this connection is widespread and not confined to a few selected and unusual loci We also have demonstrated that the targets of both DNA methylation and histone H3K9 methylation ... both size classes, with DNA transposons and LINE elements in the large size class and SINEs, tandem and inverted repeats and genes in the small size class Methylation reduction is also not attributable ... drm1/2 and ago4 were in a Ler (clk-st) and crm3 drm1/2 was in a Ws wild-type background Gene-oligo and random-PCR datasets of targets are available with a graphical interface for browsing and for...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
Ngày tải lên :
14/02/2014, 18:20
... acetylation and deacetyla2192 tion, and transient recruitment of remodellers and transcription factors A cycle of transcription commences with the recruitment of transcription factors and co-factors ... organization and higher order structure, and the chromatin structure changes that occur during locus activation I will discuss the nature of transcription factor interactions with chromatin, which ... histone acetylation and deacetylation that accompanies cycles of transcription, and highlight the special significance of histone H4 lysine 16 acetylation Active chromatin and DNase I hypersensitive...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
Ngày tải lên :
21/02/2014, 01:21
... #5336 [14], root-1 and -2 Col0 Columbia, #2336 and #2337 (Genome Systems, Inc.); 2, seedling hypocotyl CD4-13, -14, -15 and -16, #NH28, #NH25, #NH26 and #NH27 [12]; 3, rosette-1, -2 and -3 Col0 Columbia, ... nucleotide mismatches, and two sets for AtP2, AtP2a and AtP2b, with 19 mismatches and three deletions Ó FEBS 2002 AtP1b and AtP2a are identical in sequence to the genes At4g21960 and At2g37130, respectively ... Shinmyo et al [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa Most remarkably, C2, and only C2, responded strongly to wounding The AtP38...
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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf
Ngày tải lên :
21/02/2014, 15:20
... Localization and transcriptional regulation of TRAF4 (Eur J Biochem 269) 4827 Fig TNF and PMA up-regulate TRAF4 mRNA in T-cells Jurkat (A) and D23II-7 T-cells (B) as well as primary human T-cells and ... in the T-cell lines Jurkat (Fig 9A) and D23II-7 (Fig 9B) both TNF and PMA/Ionomycin, induced a significant and reproducible up-regulation of TRAF4 mRNA TNF- and PMA-induced TRAF4 expression was ... mRNA signal To obtain relative TRAF4 and L32 expression values the ratio between the signal intensities of bands of treated cells and the corresponding band of the untreated group were calculated...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
Ngày tải lên :
07/03/2014, 12:20
... of transcription factors and RNA polymerase II with the b-globin locus Undifferentiated (day 0) and differentiated (day and 12) ES cells were incubated in formaldehyde and the cross-linked chromatin ... replication and transcription Science 284, 1790–1795 31 Riggs AD (1990) DNA methylation and late replication probably aid cell memory, and type I DNA reeling could aid chromosome folding and enhancer ... (days and 12, respectively) Using chromatin immunoprecipitation (ChIP), we demonstrate that core elements of the LCR adopt a structure characteristic of transcriptionally active chromatin and recruit...
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DNA, RNA, replication, translation, and transcription
Ngày tải lên :
13/03/2014, 19:38
... origins and recruit DNA replication machinery proteins • DNA polymerase is responsible for catalyzing synthesis of new strands Replication forks form and involve a leading and a lagging strand • ... into chromatin fibers that pack in regular ways, on different length scales Replication DNA replication is semi-conservative in a daughter strand one strand from each of the initial two strands ... daughter strand one strand from each of the initial two strands end up Each strand serves as a template for a new strand New strand is formed by complementary base-pairing of the correct nucleotide plus...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... progression and maintenance of the cell cycle As MLLs and H3K4-specific methylations are well known to play critical roles in gene expression, we analyzed the dynamics and functions of MLLs and H3K4 methylation ... that MLL and H3K4 trimethylation show different dynamics during cell cycle progression MLLs dissociate and reassociate with condensed and relaxed chromatin, respectively, whereas H3K4 trimethylation ... MLL1, H3K4 trimethylation is well known to be associated with transcriptionally active euchromatin [12,41] Therefore, MLL1 and H3K4 trimethylation have been shown (by our laboratory and others)...
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Báo cáo khoa học: Schizosaccharomyces pombe positive cofactor 4 stimulates basal transcription from TATA-containing and TATA-less promoters through Mediator and transcription factor IIA ppt
Ngày tải lên :
23/03/2014, 07:20
... Mediator and TFIIA, but independent of TAFs Furthermore, PC4 is able to bind to double- and single-stranded DNA and to interact with TFIIB, TBP, TFIIA, Mediator, TFIIH and the gene-specific transcriptional ... double- and single-stranded DNA-binding activity Because the single-stranded DNA-binding activity can be competed away by a single-stranded DNA oligonucleotide, but not by a double-stranded one (and ... strand of the )35 to +6 region of Ad-MLP (B) EMSAs used 32P-labelled single-stranded DNA from the coding strand of the )35 to +6 region of Ad-MLP as the probe The double- (dsDNA) and single-stranded...
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Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
Ngày tải lên :
23/03/2014, 07:20
... apoptosis and transcription Hippi Also, expression of the mitochondrial-coded genes ND1 and ND4, the nuclear genome-coded mitochondrial genes SDHA and SDHB and the antiapoptotic genes BCL-2 and survivin ... caspase-1, caspase-9 ⁄ HIP-1 & HIPPI mediated apoptosis and transcription caspase-6, and caspase-3 Cleavage of Bid and release of cytochrome c and apoptosis-inducing factors from the mitochondria ... protein HIP-1 and its molecular partner HIPPI in the regulation of apoptosis and transcription, the two processes that are altered in HD [7,8] HIP-1 – its interacting partners and endocytosis...
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Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc
Ngày tải lên :
31/03/2014, 07:20
... single band, at 61 kDa (SASIG2) and 65 kDa (SASIG3), respectively, both in the whole-cell (lanes and 6) and chloroplast fractions (lanes and 7) It was therefore concluded that both SASIG2 and SASIG3 ... containing the rbcL promoter and flanking sequences (164 bp downstream of the transcription start site) was generated Plasmid pBS-1.7kb-B, including the promoter and both coding and noncoding regions ... each immunoblotted using antisera against SASIG2 (lanes and 4) and SASIG3 (lanes and 7), or the corresponding preimmune sera (pi) (lanes and 5), respectively Lane shows the Coomassie-stained chloroplast...
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chromatin and chromatin remodeling enzymes, part b
Ngày tải lên :
11/04/2014, 01:17
... reconstitution and analysis, and cytological methods for imaging chromatin functions in vivo Volume 376 includes electron microscopy and biophysical protocols for visualizing chromatin and detecting chromatin ... regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin templates We are exceedingly ... is calculated from the and 00 and known Re (30.1 nm) of the T3 internal standard For the unknown band(s) of interest, the and 00 are determined experimentally and the Re in each running...
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chromatin and chromatin remodeling enzymes, part c
Ngày tải lên :
11/04/2014, 01:19
... histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... Health and Human Development, Unit on Chromatin and Transcription, Bethesda, Maryland 20892 Sonja Ghidelli (14), National Institute of Child Health and Human Development, Unit on Chromatin and Transcription, ... HHT1 and HHT2 (for histone H three), HHF1 and HHF2 (for histone H four), HTA1 and HTA2 (for histone H two A) and HTB1 and HTB2 (for histone H two B) HHT1 and HHT2 encode identical H3 proteins, and...
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chromatin and chromatin remodeling enzymes, part a
Ngày tải lên :
11/04/2014, 02:02
... histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... visualizing chromatin and detecting chromatin interactions, enzymological assays for histone modifying enzymes, and immunochemical protocols for the in situ detection of histone modifications and chromatin ... and defined chromatin segments, protocols for nucleosome reconstitution and analysis, and cytological methods for imaging chromatin functions in vivo Volume 376 includes electron microscopy and...
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Báo cáo sinh học: " Genome structure and transcriptional regulation of human coronavirus NL63" docx
Ngày tải lên :
18/06/2014, 22:20
... ORF3 (3), E (4), M (5) and N (6) The right panel shows the MHV and HCoV-NL63 genome organization and the HCoV-NL63 sg-mRNAs strand during viral replication because most (-) strand RNAs are sub-genomic, ... nucleotide and G and in particular C as underrepresented nucleotides HCoV-NL63 has the most extreme nucleotide bias among the coronaviridae, with 39% U and only 14% C As a general trend, U and C seem ... ORF3 and E genes (43%) and the lowest C-count in the 1a/1b genes and the 3' UTR (13%, 14% and 14%, respectively) The N gene is most moderate in its nucleotide bias, with 21% C and 32% U, confirming...
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Báo cáo sinh học: " Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene" docx
Ngày tải lên :
19/06/2014, 08:20
... three viral transcripts (Orf427, Orf151 and Orf366) and their corresponding DNA sequence have been detected in both specific-pathogen-free (SPF) shrimps and WSSV-infected shrimps through a WSSV-specific ... early (IE) genes (ie1, ie2, and ie3) of WSSV were identified in infected shrimps [7] Identification of the IE genes and latency-associated genes can lead to better understanding of the life cycle ... by ultrafiltration and infectious titers of both stock viruses were determined by plaque assay and adjusted to be 1010 plaque-forming units (PFU)/ml Infection of SF9 cells and transduction of...
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báo cáo hóa học: " Immunological characterization and transcription profiling of peripheral blood (PB) monocytes in children with autism spectrum disorders (ASD) and specific polysaccharide antibody deficiency (SPAD): Case study" pot
Ngày tải lên :
19/06/2014, 22:20
... (IL-12p40), and without stimuli (IL-1ß, IL-6, and TNF-α) than normal controls In addition, cytokine production of ASD/SPAD PBMCs in response to T cell mitogens (IFN-γ, IL-17, and IL-12p40) and candida ... Shapiro G, Casale TB: Identifying and managing rhinitis and its subtypes: allergic and nonallergic components a consensus report and materials from the Respiratory and Allergic Disease Foundation ... ASD/non-SPAD and nonASD/SPAD children when stimulated by mitogens (PHA or Con A) (Panel A) or candida Ag (Panels B and C) PBMCs (106 cells/ml) were incubated with T cell stimulants (PHA, Con A, and candida...
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báo cáo hóa học:" Genome structure and transcriptional regulation of human coronavirus NL63" ppt
Ngày tải lên :
20/06/2014, 04:20
... ORF3 (3), E (4), M (5) and N (6) The right panel shows the MHV and HCoV-NL63 genome organization and the HCoV-NL63 sg-mRNAs strand during viral replication because most (-) strand RNAs are sub-genomic, ... nucleotide and G and in particular C as underrepresented nucleotides HCoV-NL63 has the most extreme nucleotide bias among the coronaviridae, with 39% U and only 14% C As a general trend, U and C seem ... ORF3 and E genes (43%) and the lowest C-count in the 1a/1b genes and the 3' UTR (13%, 14% and 14%, respectively) The N gene is most moderate in its nucleotide bias, with 21% C and 32% U, confirming...
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báo cáo hóa học:" Baculovirus-mediated promoter assay and transcriptional analysis of white spot syndrome virus orf427 gene" pptx
Ngày tải lên :
20/06/2014, 04:20
... three viral transcripts (Orf427, Orf151 and Orf366) and their corresponding DNA sequence have been detected in both specific-pathogen-free (SPF) shrimps and WSSV-infected shrimps through a WSSV-specific ... early (IE) genes (ie1, ie2, and ie3) of WSSV were identified in infected shrimps [7] Identification of the IE genes and latency-associated genes can lead to better understanding of the life cycle ... by ultrafiltration and infectious titers of both stock viruses were determined by plaque assay and adjusted to be 1010 plaque-forming units (PFU)/ml Infection of SF9 cells and transduction of...
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báo cáo khoa học: "Chemical and transcriptional responses of Norway spruce genotypes with different susceptibility to Heterobasidion spp. infection." ppsx
Ngày tải lên :
11/08/2014, 11:21
... expression profiles, phenolics and lignin of Sitka spruce bark and sapwood before and after wounding and inoculation with Heterobasidion annosum Physiological and Molecular Plant Pathology 2011, ... strand synthesis was performed as described by Sambrook and Russel [41] using enzymes purchased from Fermentas Double stranded cDNA of sufficient quality was pooled according to genotype and ... with the isogroups and isotigs in the reference file as a reference The count data were aligned in R and imported into the R-package DESeq [50] and normalized on number of counts and subjected to...
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