metal complexes in living systems

... isomers in such a stacking were also treated mathematically [69] II METAL- CONTAINING MACROMOLECULES IN BIOLOGICAL SYSTEMS A Metal Complexes in Living Systems The range of metals used by biological systems ... polyvinylpyridines in solution, in hydrogels and at interfaces were investigated [83] In aqueous solution linear or crosslinked polyvinylpyridines in the interaction with H2PtCl6 results in reduced ... Ferredoxin; nitrogenase Ferrodoxin, plastocyanin, P-450, azurins Plastocyanin, cytochromes, azurins Plastocyanin, insulin Vitamin B-12 Albumin Prosthetic groups, hemoglobin Toxic for myoglobin; cytochrome...

Ngày tải lên: 13/08/2014, 16:21

... insulin– insulin receptor interaction system Interaction of insulin with the 43-kDa protein Acknowledgements Similarly to the 4-kDa peptide, insulin is able to interact with the 43-kDa protein ... Tager, H.S (1987) Role of the COOHterminal B-chain domain in insulin–receptor interactions: identification of perturbations involving the insulin mainchain J Biol Chem 262, 12054–12058 20 Mirmira, ... area in insulin (Fig 7F) If the mechanism of the interaction between the 4-kDa peptide and 43-kDa protein has the minimum components of insulin–insulin receptor interaction, the area consisting...

Ngày tải lên: 17/03/2014, 03:20

... been reported that inappropriate folding of proteins or inappropriate assembly of multimeric protein complexes can in uence the surface and overall expression of these proteins in cells and can ... fluorescence intensities) and is represented in arbitrary units of CD38 per mg of total protein The protein concentration per cell was determined by lysing a known number of Ba/F3 cells and determining ... positions to the corresponding points in the p42 monomeric form We did not detect a protein spot at 7.4 in p95; however, this protein species only represented a minor form even in the CD38 p42 monomer...

Ngày tải lên: 23/03/2014, 12:20

... semipermeability system in regulating water transport in the joint by strongly binding to negatively charged proteoglycans In addition, the SAPL lining that covers the intracellular gaps may be ... lining on the cartilage surface becomes deficient the whole semipermeable system could be impaired, resulting in the abnormal accumulation of fluid inside the joint, causing joint effusion In ... that the dominating SAPL species inside the peritoneal cavity are USPC and two dominating USPC species, palmitoyl-linoleoylphosphatidylcholine (PLPC) and palmitoyl-oleoyl-phosphatidylcholine (POPC),...

Ngày tải lên: 20/06/2014, 00:20

... locking [14] This feature will lead to some interesting phenomena, such as the spin-dependent conductance [15] and the twisted RKKY interaction [16] In this study, we investigate electron tunneling ... the inhomogeneous magnetic field, and, more interestingly, the incident electron spin-oriented parallel along the interface will be flipped in the total reflection region Figure 3b shows the spin ... the invariance of the transmission with respect to ky → −ky Note that, perfect transmission always exists in the vicinity of normal incidence, i.e., this is the socalled Klein tunneling, induced...

Ngày tải lên: 20/06/2014, 20:20

Ngày tải lên: 08/08/2014, 21:20

... face erosion in the needles is interesting at least as an intensifying factor in the erosion caused by other air pollutants In the current study, the surface in the needles infection in ambient ... 200 pg/m increased the surface erosion in the material analyzed in our study Magel and Ziegler (1986) found wax plug disturbances in current needles of Picea abies after ozone and acid rain treatments ... accelerates winter desiccation and needle shedding (Lewitt, 1980; Huttunen and Laine, 1983) Structural degradation of epistomatal wax tubes causes increased stomatal occlusion and potentially inhibits...

Ngày tải lên: 09/08/2014, 04:20

... activating simultaneous or subsequent joint infections They may also have a role in rendering the joint more susceptible to clinical infections The predominant bacteria identified by culture-independent ... binding site is located 32 base pairs upstream of the M13 forward primer binding site, and the M13RAP binding site is located 39 base pairs downstream of the M13 reverse primer binding site, in ... al problem, resulting in high rates of morbidity and creating a massive economic burden longing or activating simultaneous or subsequent clinical infections Prosthetic joint infections of total...

Ngày tải lên: 09/08/2014, 10:20

... cultured in HAT medium, RPMI 1640 medium supplemented with hypoxanthine-aminopterin-thymidine (Sigma, USA) and 20% FBS, for a week before transferring to HT medium, medium without aminopterin, until ... We initially assessed MAb binding to native, intact viruses by a virus binding ELISA [21] We localized various epitopes by coating MAbs and sCD4 onto flatted-bottom 96-well plates, and adding ... immunogen to induce broadly neutralizing antibodies; this information may assist in the development of an effective HIV-1 vaccine Abbreviations CRF: circulating recombinant form; gp: glycoprotein; HRP:...

Ngày tải lên: 11/08/2014, 08:21

... buffer, pH 9.6 Following washing in PBS Tween, protein binding sites were blocked with 1% skimmed milk powder in PBS, and the washing step repeated Standards (purified HLA-DR) were kindly donated by ... HL-60 cell line maintenance and culture HL-60 cells, a human promyelocytic cell line, were maintained in complete RPMI (10% FCS, 20mM L-glutamine, 20U/ml penicillin, 100ug/ml streptomycin) HL-60 ... minutes in 1% paraformaldhyde, washed in PBS and then incubated in saponin buffer (0.04% EGTA; 0.02% NaN3; 1% heat inactivated FCS; 0.1% saponin in PBS) before incubation with antibody as above [All...

Ngày tải lên: 03/11/2012, 09:57

... distribution of metals is discussed The obtained metal concentrations in the sediments in the Philippines were compared with those in other Asian countries using the same method and instruments The ... 2.4 0.54 22 Discussion Most gasoline were unleaded in 1980 in Japan, in 2001 in the Philippines and in 2001 in Vietnam The core sample analysis of the sediment in Tokyo Bay shows the peak concentration ... contamination in receiving waters In order to investigate the contribution of roadside deposits to the sediment metal concentration increase, seven roadside deposit samples were collected in Manila,...

Ngày tải lên: 05/09/2013, 09:08

... reported in Fig and indicated by continuous and dashed lines, respectively The figure shows that the effect of binding Zn-rubredoxin on the NMR signals of the haem methyls vs the results obtained ... of cytochrome c3 when forming a complex with a partner protein, several experimental requirements had to be met in addition to maintaining slow intermolecular- and fast intramolecular-electron ... parameters in ligated proteins However, no experimental data exist on the effect of binding small ligands or proteins on these properties For cytochromes c3 these effects have only been investigated in...

Ngày tải lên: 19/02/2014, 17:20

... porphyrin moiety was inserted in the antibody pocket, three of the ortho-carboxylate substituents of the mesophenyl rings being bound to side chains of amino acids such as arginine [36] In this ... binding of ligands on the iron of Fe(porphyrin))13G10 complexes: (A) binding of H2O2 on the iron of 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding ... porphyrin inside the antibody pocket, leaving only one of its faces accessible to ligands Consequently, it is more likely that in our case, an edge-on binding of Fe(ToCPP) occurs inside the binding...

Ngày tải lên: 21/02/2014, 03:20

... important role in molecular recognition independently of scaffold proteins The generation of recombinant binding proteins by library approaches will supply new insights into protein–protein interactions, ... A-domains consisting of  35 amino acids occur in strings of multiple domains in several cell surface receptors, and are connected via several amino acid linkers Each A A-domain in the multimer binds ... proteins from X-ray and NMR analyses enables the design of and screening for small binding proteins The preparation of the small binding proteins with binding function further allows us to increase...

Ngày tải lên: 06/03/2014, 11:20

... part in binding M3 metal by forming two of the coordinating interactions In SaSTP, this residue has been replaced by Asn160 (Fig 2B) This residue is in two different conformations, depending on ... when the residues binding the metal ion in MtSTP are mutated, the Km value for metal binding was little changed These mutations included the S160A mutation, making the MtSTP binding site similar ... active site remains unclear [11] Interestingly, MtSTP has been shown to bind a third metal ion near the active site [9] A serine residue that takes part in binding the third metal ion is not...

Ngày tải lên: 07/03/2014, 09:20

... determined here using a whole cell kinetics approach, which has the advantages of: (a) maintaining the complete electron transport chain used during metal respiration; and (b) keeping the terminal ... generating Lineweaver–Burk lines with intersecting y-axis intercepts, which is the characteristic signature of competitive inhibition We calculated a Ki value of 22.5 lm, suggesting that the kinetics ... supplemented initial concentration of inhibiting metallic substrate, and IC50 is the half-maximal concentration of inhibiting metallic substrate To analyze kinetic data, we used Monod-based kinetic...

Ngày tải lên: 07/03/2014, 09:20

... the amino acids proposed to be involved in binding of the surface acarbose caused reduction of enzyme adsorption on starch, proving that these amino acids are involved in starch binding site in ... Hutchinson EG & Thornton JM (1992) Stereochemical quality of protein structure coordinates Proteins 12, 345–364 Harding MM (2002) Metal- ligand geometry relevant to proteins and in proteins: sodium ... remote ligand binding site Fig A space filling model showing the complex of glucoamylase with acarbose Both acarbose molecules are in yellow Tyr464 is in green, Asp450 in red and Arg15 in blue The...

Ngày tải lên: 07/03/2014, 12:20

... buried binding sites of the c11 rotor ring within a lipid bilayer without the presence of subunit a These results therefore reinforce our model for the rotor ring with 11 intrinsic channels linking ... Na+-translocating enzymes, accumulated data support the model proposed in [19], where the binding sites are in direct contact with the cytoplasm through individual intrinsic channels in the c ring It ... reconstituted into POPC, indicating similar membrane embedded coupling ion binding sites on their enzyme (Fig 5B) The fact that the binding site of the I tartaricus enzyme is embedded in the membrane...

Ngày tải lên: 08/03/2014, 09:20

... mechanism is independent of the interaction of HSP70 with Smac but requires the ATP-binding domain of the protein However, it ATP-binding domain of HSP70 inhibits Smac release remains to be determined ... the ATP-binding domain of HSP70 is critical for sequestering AIF in the cytosol [29] In the present study, we demonstrated that the ATP-binding domain of HSP70 was indispensable for inhibition ... Jiang et al The ATP-binding domain of HSP70 is important for the interaction of HSP70 with apoptosis signalregulating kinase (ASK1) and the inhibition of ASK1-induced apoptosis in vitro [28] Furthermore,...

Ngày tải lên: 16/03/2014, 01:20