metabolite profiling of liver tumor promoters

... spectrometric identification of lipid species 32 Lipid profiling of single deletion mutant strains 33 3.3.1 Lipid profiling of strains in a random manner 33 3.3.2 Lipid profiling of strains in a domain ... ORFs, of which 4538 have been annotated The remainder falls under the category of uncharacterized as well as dubious A comprehensive lipid profiling of the yeast would require profiling of all ... presence of an ion of m/z value 153 allows its identification as a glycerophosphate head group 32 3.3 LIPID PROFILING OF GENE DELETION MUTANTS IN YEAST The lipid profiles were obtained for a set of...

Ngày tải lên: 04/10/2015, 16:03

... clustering of the expression of these miRNAs across all of the mouse mammary tumor models indicated that the expression levels of the 22 miRNAs in the tumors were not related to the background strain of ... subtype of the tumor Results miRNAs are differentially expressed among GEM mammary tumors A custom miRNA microarray platform was used to generate miRNA expression profiles of the eight GEM models of ... expression analyses of mammary tumors have revealed that certain mouse tumor models share important similarities to subsets of human breast tumors, including proliferation [12] and tumor subtype signatures...

Ngày tải lên: 09/08/2014, 23:20

... clustering of the expression of these miRNAs across all of the mouse mammary tumor models indicated that the expression levels of the 22 miRNAs in the tumors were not related to the background strain of ... subtype of the tumor Results miRNAs are differentially expressed among GEM mammary tumors A custom miRNA microarray platform was used to generate miRNA expression profiles of the eight GEM models of ... expression analyses of mammary tumors have revealed that certain mouse tumor models share important similarities to subsets of human breast tumors, including proliferation [12] and tumor subtype signatures...

Ngày tải lên: 09/08/2014, 23:20

... cytometry analysis of tumor- infiltrating cells (I) Flow cytometry analysis of tumor- infiltrating cells (I) Leukocytes were isolated from liver parenchyma or metastatic liver tumors as described ... Results Establishment of liver metastatic models of colon cancer cells Balb/C mice were inoculated with tumor cells to determine the optimal conditions for mimicking liver metastasis of colorectal cancer ... cells (II) Flow cytometry analysis of tumor- infiltrating cells (II) Leukocytes isolated from liver parenchyma or metastatic liver tumors were stained with pairs of FITC- and PE-labeled antibodies...

Ngày tải lên: 11/08/2014, 10:23

... impact of staging of liver disease and open opportunities for new trials that take into account the patient's biological makeup Conclusions Gene expression profiling of the liver at the time of diagnosis ... presence of inflammation [18] and fibrosis [19-21] with poor clinical outcome Here, we aimed to determine whether molecular profiling of the liver identifies stages of disease at diagnosis Analysis of ... by bile duct ligation [35] Molecular profiling of liver biopsies at the time of diagnosis has been shown to differentiate the embryonic and perinatal forms of biliary atresia and identified genes...

Ngày tải lên: 11/08/2014, 12:20

... classification of human tumors using mRNA microarray profiling is an area of intense research A number of classifiers have been developed for human breast tumors, including the use of expression ... set of miRNA expression profiles for primary human breast cancer we selected 99 primary human tumors, normal breast samples and 33 breast cancer cell lines for miRNA expression profiling Tumor ... separation of normal and tumor samples, as has been described before for primary human tumors [56] However, as our study was focused on tumor subtypes, we profiled only a small number of normal...

Ngày tải lên: 14/08/2014, 08:20

... liver of D2 mice Previous studies have implicated Hamp in the severity of hemochromatosis [37,38], thus supporting recent suggestions that expression profiling can accelerate identification of ... with the upregulation of δ-aminolevulinate synthase (Alas2) in the liver of these mice Other variations of potential interest Hfe-/- D2 mice exhibit increased expression of the gene encoding 3β-hydroxysteroid ... deficiency in D2 and B6 liver Genes regulated by Hfe deficiency in D2 and B6 liver A tree view image of k-means clustering for 1,343 genes regulated by Hfe disruption in liver of D2 or B6 mice is...

Ngày tải lên: 14/08/2014, 08:20

... hormone Granulosa cell tumors Granulosa cells of ovarian follicles (member of the transforming growth factor-b family) Sertoli cells of male fetus, 140 kDa for regression of Müllerian ducts Cell ... umbilical cord blood and amniotic fluid during delivery of 56 full-termed pregnancies [39]; they found a high incidence of SCC levels above the cutoff value of ≤2.5 µg/L (30% in maternal serum and 75% ... SCC is an oncofetal antigen [39] The analysis of in vitro culture of amnion cells and amniotic membranes revealed no accumulation of SCC in the supernatant, and no mRNA expression of SCC was found...

Ngày tải lên: 13/02/2014, 06:20

... conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional structure The heavy chain of ETNF-6 ... chromatograms After a reaction time of 47.2 h, the amount of TP41-1 at a retention time of 11 decreased, because of fragmentation The fragment was observed at a retention time of 13 (indicated by an arrow) ... injection of 50 lg per mouse of the emulsion in Freund’s incomplete adjuvant (Difco Laboratories) at 2-week intervals after the first immunization A final dose of 50 lg of human hTNF-a in 100 lL of NaCl...

Ngày tải lên: 06/03/2014, 22:21

... decrease of the inflation, improvement of the balance of trade, gain of the public health and social satisfaction, significant reduction of pollution outside and inside of rooms, generation of new ... projects of retrofit (per 25th of May 2009): Retrofit polytechnic school + secondary school in Schwanenstadt Retrofit home fort he aged in Weiz Retrofit apartment house Fussenau in Dornbirn Retrofit ... development of retrofits to Passivhaus standard The largest Passivhaus retrofit objects in Germany and Austria Treated floor area of each retrofit objects to Passivhaus standard 6.1.4 6214 6.000 Retrofit...

Ngày tải lên: 17/03/2014, 00:20

... report in this article a proteomic analysis of the changes in expression levels of various enzymes of liver and kidney metabolism linked to the lack of AGT Results We first attempted to detect ... oxalate synthesis, justifying the study of changes in liver enzymes in the context of primary hyperoxaluria It could be speculated that our finding of enhanced liver gluconeogenesis in the PHI mouse ... consistent with the reliability of our comparative proteomic study To assess the tissue specificity of the liver and kidney response, we also performed western blot analysis of skeletal muscle proteins...

Ngày tải lên: 23/03/2014, 03:20

... mechanism of Zn2+- and Ca2+dependent activation of S100 proteins The antagonistic effect of Zn2+ and Ca2+ in the control of S100A2 stability provides a molecular rationale for the action of both ... S100A2-DCys have very similar T app values of 58.4 and 59.5 °C, respectively, which are m lower than the T app value of 66.6 °C of S100A2-C2S m The outlying behaviour of apo S100A2-C2S may result from ... presumably a result of the general destabilization of the protein Further contributions might come from the exposure of a hydrophobic surface on Zn2+ binding [12], making additional exposure of the hydrophobic...

Ngày tải lên: 30/03/2014, 02:20

... analysis Microscopic analysis of the livers of GCV-treated HSV-tk mice (F1 and F2) showed that the diseased livers display a number of abnormalities, including the appearance of apoptosis bodies, hepatocyte ... scattered in the liver lobule tissue, or clustered in the periportal areas (B) Liver of the wild-type mouse showed no staining (C) Liver of transgenic mouse TK5-F1-452 after 21-days of GCV treatment, ... the liver In TK3 mice, approximately 60–70% of the total hepatocytes showed HSV-tk expression, and 20–30% of the liver cells in the TK5 family gave positive results The discrepancies of levels of...

Ngày tải lên: 30/03/2014, 16:20

... deviation of the measurements Fig Dependence of the release rate of calcein from liposomes on the amount of TRAIL at pH 4.5 Various amounts of TRAIL were added to liposomes consisting of Myr2Gro-PCho ... intensity of calcein was determined to assess the amount of calcein in the liposome RESULTS Structural analyses of TRAIL by CD spectroscopy The effects of acidification on the contents of secondary ... with a significant change of tertiary structure Meanwhile, the effect of zinc ions on the conformation of TRAIL at acidic pH was investigated by analyzing the CD spectra of TRAIL, as zinc ions were...

Ngày tải lên: 31/03/2014, 08:20

... liver sections of animals treated for 46 days In contrast to control animals, in liver of thioacetamide-treated rats loss of the liver cell structure and intercellular granula, increase of connective ... concentration of albumin and the activity of the TRH-DE in the culture medium increased in a correlative manner (Fig 2) Purification of the TRH-DEs from serum and liver The membrane-bound liver TRH-DE ... Effect of thioacetamide, a hepatotoxin, on the activity of the serum TRH-DE Thioacetamide (0.03%) was either added or not to the drinking water of adult male rats At the indicated time points mL of...

Ngày tải lên: 31/03/2014, 21:21

... production of microbial metabolites LAB indeed produce a wide variety of compounds, including low molecular weight metabolites such as CO2, H2O2, organic acids, alcohols and high molecular weight metabolites, ... specific subareas of food fermentation and biological preservation, the following two basic aspects should be taken into account In view of the above-mentioned importance of microbial metabolites, ... surface in Fig 1a parallel to the [LaH]-axis (i.e., lines of equal values of pH) (Fig 1b) or parallel to the pH-axis (i.e., lines of equal values of [LaH]) (Fig 1c) From Fig 1b, it can be seen that,...

Ngày tải lên: 05/05/2014, 08:44

... Institutional Review Board of the University of Pennsylvania Tumor inoculation For intraperitoneal (i.p.) tumors, ID8-Vegf cells were injected at × 106 per mouse For subcutaneous (s.c.) tumors, a single ... control tumors reached the size of ~800 mm3, animals were sacrificed, and tumors excised and weighed In Vivo Treatment Mice were treated with i.p bolus injections of Doxil in the range of 17% ... amalgamation of IL-18 mediated immune activation, with Doxil-mediated tumoricidal activity and increased immunogenicity of the surviving tumor cell fraction in vivo Although upregulation of MHC class...

Ngày tải lên: 18/06/2014, 15:20

... use of tumor target antigens like WT1 which are crucial for tumor cell proliferation However, further studies, especially on mechanisms of immune evasion at the effector phase of the anti -tumor ... loss of the HLA-A2 allele, we analyzed HLA-A2 expression on leukemic blasts of patients In all patients more than 90% of blasts stained positive for HLA-A2 In none of the patients a difference of ... acquisition of data, analysis and interpretation of data and wrote the manuscript; AL, AN and OS have made substantial contributions to acquisition of data, analysis and interpretation of data CS...

Ngày tải lên: 18/06/2014, 16:20

... mentioned above are based on the expression of surface markers on CTCs, in particular, EpCAM Table Comparison of EpCAM and cytokeratin (CK) expression profile of tumor cells in body fluids and peripheral ... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... estimation of the number of CTCs If detection of CTCs without a classical epithelial phenotype has clinical relevance need to be determined Page of 8 Additional material Additional file 1: Expression of...

Ngày tải lên: 18/06/2014, 19:20

... failure of anti -tumor immunity [1] Given the multitude of immune modulatory factors produced by tumors, it is indeed quite likely that different subsets of MDSC may be generated in the tumor microenvironment ... dependent upon the unique profile of factors secreted by the tumor [16,17,20] Preclinical models of human tumor- induced MDSC will significantly advance knowledge of their induction and function ... subsets of MDSC have been identified that will help elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods Cell Lines and Cell Culture Tumor cell...

Ngày tải lên: 18/06/2014, 19:20