massarray™ technology for analysis of dna sequence variations

... TRƯỜNG ĐẠI HỌC NÔNG NGHIỆP HÀ NỘI KHOA CÔNG NGHỆ SINH HỌC TIN SINH HỌC DNAstar MEGA5 for analysis of biological sequence Giáo viên hướng dẫn : TS Nguyễn Đức Bách Giáo viên hướng dẫn : TS ... nhom11 Mục lục II Nội dung 1.DNAstar I Đặt vấn đề 2.Mega5 III Kết luận Web for analysis biological sequence 4/4/15 tin sinh nhom11 Liệu có phần mềm tích hợp tất nội dung DNAstar Mega5 4/4/15 tin ... DNAstar Mega5 4/4/15 tin sinh nhom11 II Nội dung 4/4/15 tin sinh nhom11 2.1 DNAstar 2.1.1 DNAstar gì? Lịch sử sáng chế DNAstar  Được thiết kế Giáo sư Di truyền học Frederick Blattner John Schroeder,...

Ngày tải lên: 04/04/2015, 18:19

... the CE applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis DNA separation by CE has been the subject of intensive research ... Electrophoresis of ΦX174 DNA under Different Applied Voltage 66 XX List of Figures Figure 2.8 Demonstration of Separation of ΦX174 DNA by CE-PGD and CE-UV 67 Figure 3.1 Design of CCD ... Electropherogram of Environmental Water by FASS-CE-CCD……… ….………… ………………………… ……… 178 XXIV List of Symbols List of Symbols α normalized length of a plug σ total spatial variance of the concentration profile for...

Ngày tải lên: 14/09/2015, 11:40

... algorithms is indicated in the first two columns of Table The maximum and median of the FDR P values CONCLUSION Signal processing for analysis of DNA microarrays for gene expression profiling is a rapidly ... paired-t test of hypotheses (1) with fcmin = at a FDR level of 20% The second stage of the “Two-stage FDR-CI” algorithm selects Ᏻ2 as a subset of Ᏻ1 at the prescribed FDR-CI level of 20% Stage of the ... impeded their use for practical experiments First, is the need for improved relevance of the fitness criterion to the scientific objectives of the experiment It is often difficult for an experimenter...

Ngày tải lên: 23/06/2014, 01:20

... dark incubator (1) 0 0 0 P O 4- P 0 0 0 0 00 00 flow rate of oxygen(mL/min) 00 Fig.2 Variations of oxygen supply rate with the changes of oxygen flow rate sampling PO4-P 3,5,7day after passing ... Operational conditions of Vial experiment condition of experiment The water of bottom layer (after the treatment by high concentrated oxygen dissolver)+soil sediment The water of bottom layer ＋soil ... (m g / d ry - g ) concentration of dissolve oxygen（mg/L) flow rate of effluent（ L/min） Fig.3 Phosphorus release in the water of bottom layer (after the treatment of high concentrated oxygen dissolver)...

Ngày tải lên: 05/09/2013, 08:40

... excess of 0.2 m phosphate buffer pH 7.5 Synthesis of the uorescent Cbl analogue CBC-244 Activation of the 5Â hydroxyl group in the a-ribofuranoside moiety of CNCbl was performed with help of 1,1Â-dicarbonyl-di-(1,2,4-triazole) ... below Competitive binding of CBC and Cbl, calculation of k+ We have tested the application of the uorescent analogue CBC as a tool for investigation of the binding kinetics of nonuorescent ligands ... retention of Cbl Additionally, a His residue substituted for water of H2OCbl, which added to protection of the ligand against reduction and coordination of other compounds The structure of TCCbl...

Ngày tải lên: 19/02/2014, 05:20

... holder) for The excitation wavelength was set at 295 nm for selective excitation of Trp residues, or at 320 nm for excitation of essDNA For analysis of the quenching of Trp fluorescence, Mhr1– DNA ... intensity of the essDNA–Mhr1 complexes For analysis of Mg2+ dependency, Mhr1–essDNA complexes were formed by addition of Mhr1 to 13 lm essDNA in 25 mm Tris ⁄ HCl (pH 7.5) in the presence of 10 mm ... of Mg2+ Circular ssDNA of / X174 (10 lM) or circular dsDNA of pUC119 (5 lM) was incubated with the Mhr1 mutants (0.2, 0.4, 0.6, 0.8 and 1.0 lM for ssDNA; 0.1, 0.2, 0.3, 0.4 and 0.6 lM for dsDNA)...

Ngày tải lên: 06/03/2014, 09:22

... HIGH FREQUENCY LIMIT OF THE PLATEAU OF THE UNDAMPED PVSS OF THE SIMPLE SHOCKS OF THE HIGH PV REGION IS SET BY THE MAXIMUM ACCELERATION OF THE SHOCK The Low Frequency Asymptote of a Zero Mean Shock ... relationship with four sets of lines, log spaced: vertical for frequency, horizontal for velocity, down and to the right for acceleration, and down and to the left for deflection Zero Mean Simple ... displacement line of about 50 inches Figure 4, our PVSS on 4CP, for that hard drive non operational shock, shows a lot of information We’ll talk more about this later, but for now you see a peak...

Ngày tải lên: 09/03/2014, 00:20

... linkage and analysis of such data, in particular for detection, interpretation, area calculation, monitoring and future estimating Therefore, this study applied GIS and remote sensing for analysis ... way of land use system • The forest area was destroyed by increasingly shifting cultivation and rubber plantation areas • Lack of an appropriate tool for decision support system in terms of land ... with registered of image 1999 (image to image) RMC= 0.14 pixels Change pixel 30m of image 1999 to 25m of image 2004 NDVI compositing utility – NDVI is useful for identifying of the green leaf...

Ngày tải lên: 17/03/2014, 11:20

... pyrophosphate sequences (supplementary Table S2), not overlapping with the initial set of sequences The consensus sequence of the Sargasso sequences is shown in Fig 4B Comparing all sequences detected ... Sargasso sequences, but many of the residue variations are identical Furthermore, the variable regions are generally located at the same sites as for the sequences in Fig 4A For a number of plant ... fingerprint in the search for further members of this family from new sequence data Analyses of the various primary structures of H+PPases (PPi synthases) raise the possibility of exploring, in molecular...

Ngày tải lên: 30/03/2014, 10:20

... SLOVAK UNIVERSITY OF TECHNOLOGY IN BRATISLAVA FACULTY OF CHEMICAL AND FOOD TECHNOLOGY SYSTEMATIC METHOD FOR ANALYSIS OF PERFORMANCE LOSS WHEN USING SIMPLIFIED MPC FORMULATIONS DIPLOMA THESIS ... the best way of simplification for our example of plant is found, which turns out to be delta input blocking Keywords: analysis of MPC, simplified MPC formulations, analysis of MPC performance ABSTRAKT ... Institute of Information Engineering, Automation and Mathematics at the Faculty of Chemical and Food Technology of the Slovak University of Technology in Bratislava, Ing Michal Kvasnica, PhD., for...

Ngày tải lên: 31/03/2014, 22:20

... backward transformation is implemented Higher order transformation can be of interest for some applications, for example, to rectify perspectival deformations as they appear in images of cylindrical ... However, for algorithms needing processing of longer lists, for example, image statistics, the influence of the CPU can be neglected fined as an 8-bit quantity Especially, for processing of color ... backward transformation and interpolation of the arbitrarily formed image regions In this context, a region is defined as a set of points Therefore, a specific region is described by a list of (target)...

Ngày tải lên: 22/06/2014, 22:20

... overview of DNA sequence analysis techniques based on correlation functions and DSP-based methods is presented in Section The motivation for the use of parametric spectral analysis methods for DNA analysis ... the role of noncoding regions It is therefore of central interest to analyze and characterize various DNA regions such as coding and noncoding sequences REVIEW OF METHODS FOR DNA SEQUENCE ANALYSIS ... the AR modeling of DNA sequences are discussed in Section 4.2 4.2 Proposed AR model-based DNA sequence analysis The AR modeling of a DNA sequence is done by first mapping the sequence into the...

Ngày tải lên: 23/06/2014, 01:20

... treatment of the semi-suspensions as well as before and after drying of the cured pastes and after vacuum treatment as well as for the cured paste before and after drying Curing of the plates was performed ... algorithm presented in Table The end -of- life criterion was when the battery reached an end -of- discharge voltage of 9.0 V for h of discharge at 50% DOD Table Quantity of water added to or removed from ... greater than that of the ®rst series of experiments The condition for equal paste density was important for determining the amount of water removed from the pastes The pastes were used for pasting...

Ngày tải lên: 05/07/2014, 21:20

... cutoff of ≥ 50) Hence each tile's position shows its degree of sequence similarity to each of the three selected databases Sequences showing similarity to only one database are not shown Sequences ... Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence analysis [48] The set of 742 clusters was ... The overall proportion of cDNAs encoding predicted signal peptides is within the 15-25% range estimated by analyis of wholegenome sequence data [50] Of all predicted signal-sequencebearing clones...

Ngày tải lên: 09/08/2014, 20:20

... http://genomebiology.com/2011/12/7/R62 regulation of these sites is critical for the maintenance of pluripotent states Conclusions We performed a comparative analysis of the genomewide DNA methylation profiles from three HESC ... of California, Los Angeles, CA 90095, USA 2Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA Interdepartmental Program in Bioinformatics, University of ... Additional file for lists of associated genes) The correlation in CpG island shores is, however, less clear (Figure S3b in Additional file 1) An analysis of the gene ontology terms for genes associated...

Ngày tải lên: 09/08/2014, 23:20

... to the different isoforms The relative strength of the segments corresponds to the abundances of the two isoforms (Figure S9 in Additional file 1) Examining levels or changes of particular genes ... not adjusted for multiple testing This should be kept in mind when setting cutoffs on the website We usually use a P-value cutoff of 10 For example, using the UCSC genes cluster for mouse (mm9) ... header of the desired field, or filtered using a text string or a numeric filter Action buttons allow for the export of the table into other software, such as R or OpenOffice (or Excel), for automatic...

Ngày tải lên: 09/08/2014, 23:20

... However, absence of a reference sequence for chickpea and the short read length of sequences (26-bp) limit the utility of this approach EST assembly cDNA SSH libraries A total of 6432 clones were ... roots of resistant genotype (ICC 4958) under drought stress Up regulated in roots of extremes bulks of RILs of HRB under drought stress Down regulated in roots of extremes bulks of RILs of HRB ... chickpea Additional file 2: Primer sequences for qPCR analysis All primer sequences used for qPCR analysis in the manuscript are listed Additional file 3: Daily NTR ratio of each well watered (WW) and...

Ngày tải lên: 11/08/2014, 11:22

... indication for the use of intraoperative navigation, the patients were assigned to one of three types of interventions according to head fixation and use of the DRF including its mode of attachment ... fixation of the head Type B1 fixation of the head (A) Patient positioned on the right side for resection of a left frontal metastasis (B) After repositioning in the prone position for resection of ... groups (e.g for differences in FRE between the different types of head fixation, as well as for differences in ∆PE in-/decrease between the different types of head positioning over the time of surgery)...

Ngày tải lên: 11/08/2014, 23:22

... Construction of DNA Templates for Transcription We used RAFL as templates DNA templates of E2s and E3s for transcription were constructed by "Split-Primer" Page of 11 (page number not for citation ... UPL-FLAGs Page of 11 (page number not for citation purposes) BMC Plant Biology 2009, 9:39 Figure of (UPL7 and recombinant Arabidopsis HECT-type E3 ligases Analysis UPL5) Analysis of recombinant ... A, Production of FLAG-tagged recombinant UPL proteins was detected by immunoblot analysis For analysis, μl of crude recombinant UPL proteins were loaded, and detected by immunoblot analysis using...

Ngày tải lên: 12/08/2014, 03:20

... gliadin sequences were obtained by amplification of either genomic DNA [20,21] or cDNA [22] and thus reflect variants of sequences already in the database In addition, there are only 20 cDNA sequences ... obtained from an analysis of Butte 86 flour and associate individual gluten proteins with the sequences of specific genes Results Assembly of Butte 86 contigs for gamma gliadins A total of 153 ESTs ... accounted for 43.4% of Table Evidence for expression of sequences encoding gamma gliadins with odd numbers of cysteine residues Protein accession # Source # Cysteine residues Region of missing/...

Ngày tải lên: 12/08/2014, 03:21