mammalian target of rapamycin mtor

... sensitive) consists of mTOR and Raptor, and its activation results in phosphorylation of p70S6 and 4E-BP1 mTORC2 consists of mTOR and the rapamycin- insensitive companion of mTOR (Rictor), and ... Elfiky et al.: Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer Journal of Translational Medicine 2011 9:133 ... combinations of rapamycin and LY294002) Viability of cells treated with LY294002, rapamycin or the combination is calculated as a percent of the viability of the untreated (control) cells Activity of the...

Ngày tải lên: 18/06/2014, 22:20

... sensitive) consists of mTOR and Raptor, and its activation results in phosphorylation of p70S6 and 4E-BP1 mTORC2 consists of mTOR and the rapamycin- insensitive companion of mTOR (Rictor), and ... Elfiky et al.: Characterization and targeting of phosphatidylinositol-3 kinase (PI3K) and mammalian target of rapamycin (mTOR) in renal cell cancer Journal of Translational Medicine 2011 9:133 ... combinations of rapamycin and LY294002) Viability of cells treated with LY294002, rapamycin or the combination is calculated as a percent of the viability of the untreated (control) cells Activity of the...

Ngày tải lên: 20/06/2014, 04:20

... humans, mTORC1 consists of Raptor (Regulatory associated protein of mTOR) and mLST8 (mammalian Lethal with SEC13 protein 8), and mTORC2 consists of Rictor (Rapamycin insensitive companion of mTOR) , ... inhibitors of mTOR that target both mTORC1 and mTORC2 This new generation of mTOR inhibitors is commonly referred to as second generation mTOR inhibitors, or TORKinibs because they target the ... studies of rapamycin are yielding new and interesting mechanisms of action of this drug, outside of its action on mTOR Noteworthy of these are the handful of studies that have reported that rapamycin...

Ngày tải lên: 05/10/2015, 13:53

... has demonstrated that inhibition of the mammalian target of rapamycin (mTOR) is capable of fostering the selective survival and expansion of Foxp3+ Tregs [13] mTOR is an evolutionarily conserved ... activate mTOR in a phosphoinositide-3kinase (PI3K)-Akt-dependent fashion In the presence of rapamycin, the PI3K-Akt -mTOR signaling pathway is inhibited, and multiple downstream targets of mTOR, ... The pmTOR+ cells were located in both the epithelium and submucosa of nasal polyps; (B), Expression of pmTOR was primarily located in the submucosa of nasal polyps; (C) Immunostaining of pmTOR...

Ngày tải lên: 12/08/2014, 14:20

... 2135 A molecular target of SQAGs A S Aoki et al A B Fig Inhibition of the microtuble depolymerization activity of MCAK184 by SQAG in vitro In all assays, 120 nM of MCAK, 1500 nM of paclitaxel stabilized ... immobilize on the control well Increase rate of recovery for control ¼ titer of SQAG immobilized well ⁄ titer of control well Table SPR analysis of the binding of SQAGs to the immobilized peptide, MCAK184 ... Occurrence of animal sulfolipid in the gametes of sea urchins Jpn J Exp Med 35, 315–318 Isono Y & Nagai Y (1966) Biochemistry of glycolipids of sea urchin gametes I Separation and characterization of...

Ngày tải lên: 19/02/2014, 17:20

... autophosphorylation activity, loss -of- function (kinase-inactive) forms of the MAPKs were produced as GST-lofMPK3 and GSTlofMPK6 However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation ... increasing amounts of PTI1-4 enhanced the autophosphorylation activity of OXI1 (Fig 4B) as well as the transphosphorylation of MBP Simply by incubating the two proteins over a period of 50 Time (h) ... K45R lofMPK3 MPK6 lofMPK6 K45R K45R K45R OXI PTI OXI PTI HIS: - OXI PTI OXI PTI 30 15 30 + + + + + – -72 -55 HIS-OXI1 > < MBP HIS-PTI1-4: 15 30 45 – < HIS-OXI1 GST-MPKs < GST B OXI1 lof lof -...

Ngày tải lên: 14/02/2014, 19:20

... composition of the extracellular matrix of human oocytes and cumulus masses Hum Reprod 1992, 7:391-398 Dandekar P, Talbot P: Perivitelline space of mammalian oocytes: extracellular matrix of unfertilized ... adult females In the group of young females, but not the adults, development of the embryos was also highly synchronous this was accompanied by increased rate of movement of eggs or microspheres ... evidence supporting the idea that the oviduct is targeted by cigarette smoke (1) In vivo evidence that the oviduct is a target of smoke Direct inhalation of whole smoke has been shown in several studies...

Ngày tải lên: 05/03/2014, 17:20

... complexes exemplify mechanisms of PP2A regulation which are independent of the canonical A and B regulatory subunits Type 2A phosphatases are key players in the yeast target of rapamycin (TOR) signaling ... regulator of the rapamycin- sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator of the activity of type 2A ... characterization of TOR signaling pathway regulator-like (TIPRL; TIP41), the mammalian ortholog of Tip41, may provide clues to better understand the regulation of type 2A phosphatases and mTOR signaling...

Ngày tải lên: 07/03/2014, 05:20

... al three putative categories of targets, Six1-specific targets, Six4-specific targets and common targets We then focused our analysis on one of the typical common target genes, sodium-potassium-chloride ... Six4W263R were considered potential Six4 target genes A total of 363 Six1 target genes and 149 Six4 targets genes were identified Of these, 63 were common target genes The data were deposited in ... regions [16] To explore the target genes of Six1 and Six4 in the physiological context of development, we took advantage of using the cell line mK4 isolated from the kidneys of transgenic mice that...

Ngày tải lên: 07/03/2014, 17:20

... al SerpinA3 is a novel target of Nur77 Table Information of candidate genes with NBRE in the promoter regions NBRE position, the position of the first and the last base of NBRE to TSS; direction: ... SerpinA3 is a novel target of Nur77 Y Zhao et al A B C Fig Structure and nucleotide sequences of the proximal promoter region of human SerpinA3 (A) Schematic presentation of the 5¢ SerpinA3 promoter ... and protein levels of SerpinA3 were increased; when the expression of Nur77 was suppressed, the expression of SerpinA3 was reduced Second, analyses of the regulatory elements of SerpinA3 demonstrated...

Ngày tải lên: 23/03/2014, 07:20

... direct target gene of CUX1 Expression analyses show a reciprocal expression pattern of CUX1 and PLZF among CRC cell lines and normal isolated colonocytes Because of the well-documented role of PLZF ... with the production of multiple isoforms of the proteins, as reported previously (Fig 4B) [35] The murine Cux1 protein was shorter than the human CUX1 protein because of the use of an N-terminally ... results in a hypomorphic allele because of the production of an N-terminal, 160-kDa truncated form of Cux1 in mutant mice, as already observed for other CUX1 gene targets [9,16] To further determine...

Ngày tải lên: 29/03/2014, 21:20

Ngày tải lên: 06/06/2014, 13:31

... amplification was done in 50 μL of PCR mixture containing μL of the cDNA mixture, μL of KOD Plus DNA polymerase (TOYOBO, Osaka, Japan) and 15 pmol of primers For specific detection of AMACR, forward primer ... http://www.translational-medicine.com/content/7/1/103 Figure Expression profiles of AMACR as assessed by RT-PCR Expression profiles of AMACR as assessed by RT-PCR A Expression of AMACR in normal tissues including heart, brain, ... profile of AMACR in normal adult tissues by RT-PCR was difined We detected the overt expression of β-actin mRNA and AMACR mRNA in prostate cancer line LNCaP, but only very weak expression of...

Ngày tải lên: 18/06/2014, 15:20

... in cell culture)[22,23] The results of our recent study indicated a strong proof of the reliability of iTRAQ Page of 10 approach in the proteomics analysis of PCa Periostin as an up-regulated ... expression and function of Periostin in PCa The expression of Periostin was verificated by western blotting The results revealed a significant increase of the amount of Periostin in PCa compared ... lines:LNCap,DU-145,PC3,22RV1 were obtained from the Cell Bank of Chinese Academy of Sciences(Shanghai) and maintained in RPMI 1640 with 10% of fetal bovine serum, 100 u/mL of penicillin, and 50 mg/mL streptomycin...

Ngày tải lên: 18/06/2014, 19:20

... in cell culture)[22,23] The results of our recent study indicated a strong proof of the reliability of iTRAQ Page of 10 approach in the proteomics analysis of PCa Periostin as an up-regulated ... expression and function of Periostin in PCa The expression of Periostin was verificated by western blotting The results revealed a significant increase of the amount of Periostin in PCa compared ... lines:LNCap,DU-145,PC3,22RV1 were obtained from the Cell Bank of Chinese Academy of Sciences(Shanghai) and maintained in RPMI 1640 with 10% of fetal bovine serum, 100 u/mL of penicillin, and 50 mg/mL streptomycin...

Ngày tải lên: 20/06/2014, 04:20

... distributed along the full length of the molecules All of these epitopes share basic properties and are exposed on the surface of the protein, rendering them antigenic [19] One of the B-cell epitopes on ... carboxyl-terminus of SmD1 [21,22] R20 The aims of the present study were to develop a peptidebased ELISA system for the detection of anti-Sm antibodies and to evaluate the diagnostic properties of the peptide ... instructions for use Blood donors exhibited a reactivity range of 0.4–11.5 U/ml, a mean value of 2.2 U/ml and a standard deviation of 1.2 U/ml The cutoff was set at 13 U/ml following receiver operating...

Ngày tải lên: 09/08/2014, 06:22

... neither of which are good models for ASM physiology (since their physiology is quite different from that of ASM) Basic pharmacology of excitation of ASM Knowledge of the innervations of the airway ... localization of caldesmon and calponin, leading to removal of inhibition of actin, resulting in contraction Evolution of asthma therapy By and large, the advances made in our understanding of excitation-contraction ... through stimulation of the phosphoinositide signalling cascade and of the Rho/ROCK-mediated change in Ca2+-sensitivity This led to the development of blockers of those receptors and of leukotriene...

Ngày tải lên: 12/08/2014, 16:20

... deviations of blood glucose levels were used as markers of glycemic variability A standard deviation of blood glucose levels of above 20 mg/dL was associated with a 9.6-fold increase of mortality ... coefficient of variation of glycemia as markers of glycemic variability We found that such variability was independently associated with ICU and hospital mortality rates as well as length of ICU stay ... of variability of glycemia in critically ill patients In this four-center retrospective study of 7,049 critically ill patients, we found that glycemic variability was an important dimension of...

Ngày tải lên: 13/08/2014, 16:20

... pillar of support in times of troubles Chang Ti Ling January 2009 ii TABLE OF CONTENTS ACKNOWLEDGEMENTS Page i TABLE OF CONTENTS iii SUMMARY vii LIST OF TABLES ix LIST OF FIGURES x LIST OF ABBREVATIONS ... THE DIRECT TARGET OF RUNX3 IN GASTRIC EPITHELIAL CELLS CHANG TI LING (M.Sc., National University of Malaysia) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE ... APPENDICES Genomic Structure of Runx3, Structure of The Targeting 125 Vector For Homologous Recombination, and The Gene Structure of The Targeted Locus Full Length Sequence of hclaudin-1 Promoter (1530...

Ngày tải lên: 11/09/2015, 09:00

... Mechanism of action of rapa Rapamycin is a known inhibitor of the mammalian Target Of Rapamycin (mTOR) pathway The mechanism of this inhibition has been well established 1.4.4.1 Structure of the mTOR ... comparison of transcriptome profiles of MV411 and THP-1 75 Figure 4.3 Hierarchical clustering of transcriptomic profiles of microarray data 78 Figure 4.4 Linear regression analysis of fold-changes of ... 13 1.4.2 Rapamycin as an immunosuppressant 16 1.4.3 Antifungal properties of rapamycin 16 1.4.4 Mechanism of action of rapa 17 1.4.4.1 Structure of the mTOR protein...

Ngày tải lên: 11/09/2015, 09:18