mammalian cell sorting with sedimentation field flow fractionation
field flow fractionation in biopolymer analysis
Ngày tải lên :
29/05/2014, 16:16
... 207 Susanne K Wiedmer and Gebrenegus Yohannes 15 Mammalian Cell Sorting with Sedimentation Field- Flow Fractionation ... Dukjin Kang Field- Flow Fractionation in Therapeutic Protein Development 73 Joey Pollastrini, Linda O Narhi, Yijia Jiang, and Shawn Cao Assessing and Improving Asymmetric Flow Field- Flow Fractionation ... Isolation and Characterization of Cells by Dielectrophoretic Field- Flow Fractionation 255 Peter R.C Gascoyne 17 Field- Flow Fractionation Coupled to Inductively...
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Analysis of transport phenomena and electrochemical reactions in a micro PEM fuel cell with nature inspired flow field design
Ngày tải lên :
09/09/2015, 10:17
... densities between an interdigitated flow field and a conventional flow field However, at higher current densities, a fuel cell with an interdigitated flow field has a limiting current, which ... micro PEM fuel cell consist of cathode and anode gas flow fields, and the MEA are shown in Figure 2.2 Model equations 2.2.1 Gas flow channels In the fuel cell channels, the gas -flow field is obtained ... PEM fuel cell with nature inspired gas flow channels Table Geometrical and operational parameters for base case conditions Parameter Symbol Value Cell length Lcell 5000 µm Cell width Wcell 5000...
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Báo cáo y học: "Comparison of capillary based microflurometric assay for CD4+ T cell count estimation with dual platform Flow cytometr" pps
Ngày tải lên :
10/08/2014, 05:20
... capillary as a flow cell unlike the conventional flow cytometry The whole blood sample is stained with antiCD3+ (T cell surface marker; present on all T cells) antibodies tagged with phycoerythrin ... CD4+ T cell counts < 350 cells/mm3 to 0.771 for < 500 cells/mm3 CD4+ T cell counts The Bland-Altman plots also showed that the variation in CD4+ T cell counts between the two methods was within ... CD4+ T cells was 306 ± 207 cells/mm3 by the flowcytometry and 290 ± 203 cells/mm3 by the EasyCD4 System The CD4+ T cell counts estimated in the population under study ranged from 10 to 1110 cells/mm3...
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báo cáo khoa học: "Non-destructive on-chip cell sorting system with real-time microscopic image processing" pptx
Ngày tải lên :
11/08/2014, 00:22
... Figure Principle of our cell sorting method Principle of our cell sorting method (a) without DC voltage irradiation: sample cells flow down within the central laminar flow, (b) with DC voltage (up ... image of sorting COS cells Micrograph image of sorting COS cells A series of (a1, 2, 3) with DC voltage application, a series of (b-1, 2, 3) without voltage application cells' shift from one flow ... Figure The design of cell sorting system The design of cell sorting system (a) schematic view of the cell sorter system (b) schematic view of on-chip cell sorter disadvantages with regard to their...
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báo cáo khoa học: " Genetic control of mammalian T-cell proliferation with a synthetic RNA regulatory system - illusion or reality?" pps
Ngày tải lên :
11/08/2014, 12:21
... new era in adoptive T -cell therapy because of the increase in the amount and survival of infused T cells found with this system Their system for the control of mammalian T -cell proliferation is ... primary human central memory T cells with this system In vitro results showed that the population of live central memory T cells increased by 24% and that apoptotic cell popu lation was decreased ... of mammalian T -cell proliferation with synthetic RNA regulatory systems Proc Natl Acad Sci USA 2010, 107:8531-8536 doi:10.1186/gm198 Cite this article as: Lee SK, Calin GA: Genetic control of mammalian...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
Ngày tải lên :
18/02/2014, 16:20
... (pro)-apoptotic stimuli in mammalian cells with some focus on the hepatocyte A more general treatize on osmosensing and osmosignaling is provided elsewhere [9] Osmosensing in mammalian cells The investigation ... that cell volume fluctuations release signals of (patho)physiological relevance The understanding of how cell swelling integrates into the cell cycle machinery and how cell shrinkage sensitizes cells ... phenotype [20] Cell swelling due to isoosmotic osmolyte uptake could be one mechanism that contributes to cell cycle progression It was shown that cell water increases during the cell cycle of...
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Báo cáo khoa học: Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding Pallavi Srivastava and Dulal Panda ppt
Ngày tải lên :
07/03/2014, 05:20
... induced apoptosis in mammalian cells HeLa cells were incubated without or with different concentrations of rotenone for 12 h and stained with annexin V and PI Panel shows cell morphology using ... panels and Cells stained with annexin V (green) indicated early apoptotic cells and PI-stained cells (red) indicated late apoptotic ⁄ necrotic cells apoptosis [22] Staining the cells with both ... stained with PI only and approximately 23% of the cells were stained with both annexin V and PI At lm, approximately 3% of the cells were stained with only annexin V, approximately 46% of the cells...
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Báo cáo khoa học: The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response pptx
Ngày tải lên :
07/03/2014, 12:20
... in K562 cells treated with BA or HE and subjected to heat stress Cells were treated with various concentrations of BA or HE for h at different temperatures After a h recovery period, the cells ... is expressed in mammalian cells The loss of enzymatic activity correlates with the loss of its solu- Fig Flow cytometric analysis of mitochondrial membrane potential of K562 cells after heat ... after heat treatment, or incubation with BA or CCCP Cells were left untreated or treated with BA, heat or CCCP for h as indicated Cells were then stained with JC-1 and assayed by flow cytometry...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
Ngày tải lên :
07/03/2014, 16:20
... molecular properties with the staphylococcal enterotoxins SEB and SEC than with other streptococcal SAgs [19] Cellular proliferation studies show disagreement about which TCR bearing T -cell subsets are ... inoculated with 10 mL overnight culture and grown with shaking at 37 °C to an attenuance of 0.8 at 600 nm TCR expression was induced with mM isopropyl thio-b-Dgalactoside for 3–5 h Cells were ... Ser [SSA(C26S)] Different SSA preparations were used to study T -cell proliferation capacity with human peripheral blood mononuclear cells (PBMCs) The binding of SSA and mutant C26S to soluble TCR...
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Báo cáo khoa học: Plant RMR proteins: unique vacuolar sorting receptors that couple ligand sorting with membrane internalization pdf
Ngày tải lên :
15/03/2014, 00:20
... This recycling mechanism makes the sorting process efficient, in that one receptor can participate in sorting multiple ligand molecules In plant cells, the vacuolar sorting receptor (VSR) proteins ... functions in proteins from both mammalian cells and plants, such as functioning as transcriptional regulators [35,36] and as a ubiquitin–protein ligase [37–40] In mammalian cells, the function of the ... ligand sorting How could RMR proteins serve as efficient sorting receptors if they not recycle? The aggregation model for storage protein sorting [58] may provide an explanation By interacting with...
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Báo cáo khoa học: Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex doc
Ngày tải lên :
15/03/2014, 09:20
... somewhere in the G1-phase (a) cells pulse treated (1 h) with cycloheximide early in the cell cycle (before RP) immediately suffer a long delay in the cell division cycle; (b) cells treated late in the ... G1-S transition of the mammalian cell cycle Biotechnol Bioeng 65, 631–637 Aguda B & Tang Y (1999) The kinetic origins of the restriction point in the mammalian cell cycle Cell Prolifer 32, 321–335 ... dynamical model of mammalian G1 cell cycle progression Mol Syst Biol 3, 84, doi:10.1038/msb4100126 10 Zetterberg A & Larsson O (1995) Cell cycle progression and growth in mammalian cells: kinetic...
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A course in fluid mechanics with vector field theory d prieve
Ngày tải lên :
17/03/2014, 13:48
... outflow and negative for inflow • ∫A n.v da = net volumetric flowrate out of enclosed volume (cm3/s) This is also positive for a net outflow and negative for a net inflow • (1/V) ∫A n.v da = flowrate ... force fields, like the gravitational force or an electrostatic force field Of course, in this course, the vector field of greatest interest is: Fluid Velocity as a Vector Field Consider steady flow ... vz VECTOR FIELDS A vector field is defined just like a scalar field, except that it's a vector Namely, a vector field is a position-dependent vector: v = v(r) Common examples of vector fields include...
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Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc
Ngày tải lên :
22/03/2014, 21:21
... display [39,40] Library cells expressing intact IgGs specifically labeled with fluorescently conjugated antigen are readily distinguished and isolated by fluorescence-activated cell sorting (FACS) Unlike ... analysis of individual cells, allowing single -cell resolution for selection Although FACS is a very powerful 2292 high-throughput screening methodology, sorting a library > 109 cells using FACS is ... preenriches target cells from the library prior to subsequent rounds of FACS Specifcally, E coli ⁄ F¢ cells expressing soluble IgGs in the periplasm (E-clonal cells) are simultaneously infected with the...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... Analysis of eGFP expression in 293 cells by flow cytometry 293 cell pools stably transfected with either pEBTet ⁄ eGFP or pEBTetD ⁄ eGFP were cultured with or without lgÆmL)1 doxycycline for 20 ... analysis: regulation of transcription with pEBTet ⁄ OCT1h and pEBTetD ⁄ OCT1h mRNA was isolated from stably transfected 293 cell pools that had been cultured with or without lgÆmL)1 doxycycline for ... cm2) For all subsequent analysis, pooled cells were used With seven cDNAs coding for transport proteins, northern blot analysis of 293 cells transfected with the respective pEBTet plasmids consistently...
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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc
Ngày tải lên :
30/03/2014, 20:20
... cyclin mRNAs in enriched cell cycle fractions of mouse EL4 cells Cells were separated either by elutriation or by cell sorting into G1, S and G2 ⁄ M phase fractions (A) Cells were separated by ... elutriated cells The cell cycle distribution of the cell fractions is shown in Fig 5219 Cell cycle regulation of mouse cyclin mRNAs A Penelova et al Fig Cell cultures of mouse EL4 cell fractions ... attributed to a lack of cell synchronization or cell viability (Fig 2) Two independent methods, elutriation and cell sorting gave excellent separation between G1, S and G2 ⁄ M cells and indicate overall...
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Báo cáo hóa học: " Differential sensitivity of melanoma cell lines with BRAFV600E mutation to the specific Raf inhibitor PLX4032" potx
Ngày tải lên :
18/06/2014, 16:20
... secondary mutations in this group of cell lines, with one cell line having a mutation in EGFR, and one cell line with a mutation in AKT (Table 1) In addition, the M257 cell line, which is wild type ... pathway and melanoma cell line viability Melanoma cell lines treated with μM PLX4032 for 20 hours were stained with pErk antibody and analyzed by flow cytometry a) Representative flow cytometry histogram ... PLX4032 on cell cycle and apoptosis a-c) Melanoma cell lines were cultured with μM of PLX4032 for 20 hours and stained with propidium iodide for cell cycle analysis gated on live cells a) NRAS...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên :
18/06/2014, 19:20
... mutant cell lines with antagonistic effects of vemurafenib combined with metformin As with the BRAFV600E mutant cell lines, effects on pACC followed the effects of single agent metformin with little ... vemurafenib inhibited the cell cycle with a G1 arrest in a BRAFV600E-restricted fashion, with an effect less pronounced in the naturally resistant M308 cell line (Figure 2b) There was no cell cycle arrest ... and analyzed by flow cytometry a) Cell cycle analysis of M263 treated with DMSO, vemurafenib, metformin, and the combination b) Cell cycle changes after single agent vemurafenib c) Cell cycle changes...
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Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
Ngày tải lên :
20/06/2014, 01:20
... is expressed in mammalian cells following a FV3 infection, the mammalian cell lines BGMK (green monkey) and HeLa (human), as well as an ectothermic cell line, FHM were infected with FV3 at a multiplicity ... infectious virions in BGMK cells FV3 produces infectious virions in BGMK cells BGMK cells were mock infected (A) or infected with FV3 at any MOI of (B) 48 hours post-infection cells were harvested ... be expressed in mammalian cell lines However, essential viral genes must express in mammalian cell lines since the virus is able to infect and successfully replicate in many cell lines, including...
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Báo cáo hóa học: " Research Article Power-Controlled CDMA Cell Sectorization with Multiuser Detection: A Comprehensive Analysis on Uplink and Downlink" doc
Ngày tải lên :
22/06/2014, 06:20
... system in Figure Total transmit power is in watts Method Uplink with uplink OS Uplink with downlink OS Downlink with downlink OS Downlink with uplink OS Total trans power 1.3107 1.3239 1.1781 1.2143 ... 1) Table 2: Results for the system in Figure Method Uplink with uplink OS Uplink with downlink OS Downlink with downlink OS Downlink with uplink OS Total trans power 5.1570 5.8712 4.9123 5.2204 ... solution that yields the minimum (cell) total power is found, we move the boundaries of the sector with the minimum total power to include users from neighboring cells, in an effort to try to shift...
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