liquid liquid phase separation in protein solutions in a crowded environment

... so as to provide a conducting surface to avoid sample charging which would result in shifting of the binding energy values The samples are irradiated by UV in an ultra high vacuum chamber and ... electrical instability arising from injection-dedoping of PEDT can be reversed with chemical re-doping, and hence a simple chemically-erasable read-only memory can be fabricated We measured using transient ... (i.e., a form of “electron damage”) at large applied electric fields We have characterized this process using Raman, infrared, charge-modulation, and impedance spectroscopies This instability has an...

Ngày tải lên: 14/09/2015, 14:06

... light-harvesting cofactors in DNA photolyases [18–20] DNA photolyase of E coli is typically isolated as a blue-coloured protein carrying a neutral flavin radical, FADH•, as a chromophore This catalytically ... photorepair is also accompanied by certain additional difference bands in the spectral range of 1520–1540 cm)1 A preliminary kinetic analysis shows that these bands appeared at a slower rate as compared ... PF, Okamura T, Hirata Y, Mataga N & Sancar A (1991) Determination of rates and yields of interchromophore (folate fi flavin) energy-transfer and intermolecular (flavin fi DNA) electron-transfer in Escherichia...

Ngày tải lên: 16/03/2014, 18:20

... 15:780-788 Ramakrishnan N, Xia Y, Bidthanapally A: Polarized IR Microscopic Imaging of Articular Cartilage Phys Med Biol 2007, 52:4601-4614 Ramakrishnan N, Xia Y, Bidthanapally A, Lu M: Determination ... of cartilage using Fourier-Transform Infrared Imaging (FTIRI) [12-19] show that infrared techniques with imaging capability have the potential to provide quantitative information about chemical ... section in this article, Figure 1a) In these anisotropy studies, multiple infrared imaging data were acquired, each for a different infrared polarization state in the 0°-180° angular space Subsequent...

Ngày tải lên: 20/06/2014, 01:20

... rapid and nondestructive technique that is adaptable for solids, liquids, and gases with a minimal sample preparation and can be used for both qualitative identification and the quantitative analysis ... Biomolecular features like conformational state, side chain length and inter/intra chain bondings can be measured easily using infrared spectroscopy Recently, the application of infrared spectroscopy in ... secondary structure of proteins reveal increased amount of beta sheet in necrotic area of carcinoma as compared to alpha helix [24] Moreover the bands in the protein region are disturbed in the...

Ngày tải lên: 20/06/2014, 07:20

... 1.1 Gas phase kinetics The study of elementary gas -phase reaction kinetics, a venerable area of chemical investigation, continues to play a prominent role in our understanding of fundamental chemistry ... Keeping the R· and changing the reactant in order to form a more stable product radical will also give rise to a larger rate constant Since the fundamental factor in determining radical stability results ... Chemical Kinetics and Photochemical Data for Use in Stratospheric Modeling Pasadena, CA: Jet Propulsion Lab., 266, 1997 M C Lin, Advan Chem Phys., 42, 13, 1980 G S Tyndall and A R Ravishankara, Int...

Ngày tải lên: 15/09/2015, 17:11

... if a longer data record is available A number of problems have to be avoided in performing non-parametric spectral analysis such as aliasing, finite data length, spectral leakage, and spectral ... such as rectangular, Hanning, and Hamming have relatively narrow mainlobe compared with Blackman or Kaiser windows Unfortunately, the first three windows have relatively high sidelobes, thus having ... results in a particular stage have magnitude less than 1, no scaling is necessary at that stage Otherwise, all the inputs of that stage have to be scaled by 1/2 The conditional scaling technique achieves...

Ngày tải lên: 28/10/2013, 05:15

... cyanide and in direct comparison to previous resonance Raman data it can be concluded that the hemes a and a3 have a similar structural environment comparised with bovine heart and P denitrificans ... the protein was equilibrated with an initial potential at the electrode, and single beam spectra in the VIS and IR range were recorded A potential step to the final potential was then applied, and ... cm)1 Aspartic and Glutamic acid side chains The m(C¼O) mode of protonated aspartic and glutamic side chains absorb typically above 1710 cm)1, the exact absorption depending on the hydrogen bonding...

Ngày tải lên: 21/02/2014, 15:20

... inhibitor atoms Baverage (A2 ) Protein main chain atoms Protein side chain atoms All protein atoms Inhibitor atoms Water atoms Other atoms All atoms PDB code Methyl 4,4II,4III,4IVtetrathio -a- D-xylopentaoside ... Thioxylo-oligosaccharide binding to xylanases J Janis et al ă determination of binding constants because of their low abundance and the fact that no peaks representing dimers were actually observed at ligand ... basis of the ESI FT-ICR data, CTX contained 196 amino-acid residues with an N-terminal pyrrolidonecarboxylic acid and glycosylation by a single GlcNAc; neither the last ve C-terminal amino acids...

Ngày tải lên: 07/03/2014, 17:20

... of cat-f obtained in D2O buffer at 25 °C and the same sample at 80 °C It can be seen that heating induced a very substantial increase at 1616 and 1683 cm)1, and a very substantial decrease in ... spectral widening is taking place [40], as occurs during denaturation Finally, a small positive peak correlated 1617 and 1684 cm)1, and this again may be interpreted as the simultaneous increase in ... discrimination between alpha- and 3(10)-helices in globular proteins Reexamination of amide I infrared bands of alpha-lactalbumin and their assignment to secondary structures Int J Pept Protein Res...

Ngày tải lên: 16/03/2014, 13:20

... Brennan, L E Angular accuracy of phased array radar IRE Trans Antennas Propagation AP-9 (May 1961), 268–275 FIG 27 The first main lobe, at 1000 Hz, is masking the second main lobe centered at 400 ... processing and electromagnetic compatibility TAPAN KUMAR SARKAR was born in Calcutta, India, on August 2, 1948 He received the B Tech degree from the Indian Institute of Technology, Kharagpur, India, ... that applying windowing in the Fourier Transform provides unbiased estimates at REFERENCES Hua, Y., and Sarkar, T K Matrix pencil method for estimating parameters of exponentially damped/undamped...

Ngày tải lên: 26/03/2014, 00:29

... plasmon band); and on the other hand, Ag nanodiscs have several resonance modes in the absorption spectra: (1) dipolar in- plane resonance, (2) dipolar out-of-plane resonance located; (3) quadrupolar ... to have a value of 1.34 at all wavelengths, close to that of water Figure illustrates that the DDA calculations are almost in good agreement with the results of the Mie scattering theory and also ... nanorods is in the range from to Accordingly, we simulate the extinction spectra of nanorod using aspect ratio 3, 4, and and the simulated spectra are shown in Fig 5b In addition to the surface...

Ngày tải lên: 21/06/2014, 17:20

... Electrical and Computer Engineering, vol 3, pp 1273–1276, Niagara Falls, Canada, May 2004 [20] P L Ainsleigh and N Kehtarnavaz, “Characterization of transient wandering tones by dynamic modeling ... autocorrelations and the fractional Fourier transform are used to estimate the features which can characterize a measured marine-mammal vocalization In [21], sinewave analysis and synthesis is done based ... Tsiakoulis and A Potamianos, “Statistical analysis of amplitude modulation in speech signals using an AM-FM model,” in Proceedings of IEEE International Conference on Acoustics, Speech and Signal...

Ngày tải lên: 21/06/2014, 20:20

... is a singlet consistent with the formation of a dication as determined before.23 All signals disappeared after the sample was warmed to room temperature and measured again at 77 K Recombination ... of Car shows a broad peak with a maximum at 450 nm and no other peaks The formation of Car•+ and Car2+ in CH2Cl2 are indicated by peaks appearing around 1000 or 800 nm respectively According ... counteranion can cause a large blue shift in the λmax of Car•+ The polar environment of MCM-41 may also cause a large blue shift in the λmax of Car•+ and Car2+ For example,58 the λmax of Car•+(I) in...

Ngày tải lên: 29/07/2015, 02:43

... Partial differential equation In mathematics, a partial differential equation is a type of differential equation that involves an unknown function of several independent variables and the partial ... non-linear Poisson-type equation, including a Laplace operator and a non-linear term, is solved by the FFTM An iterative scheme is used in the fast non-linear solver In each iteration, a Poisson ... advantage enables BEM to become popular since the 1980s, and it has been applied successfully in many areas in science and engineering including heat transfer [32], fluid mechanics [68, 67], acoustics...

Ngày tải lên: 11/09/2015, 16:06

... containing the logarithm singularity, and the other is nonsingular The nonsingular part can be evaluated using the standard Gaussian quadrature, while the singular one can be evaluated with logarithmic ... form singularity solutions for many practical engineering applications, such as fracture in a bi-material interface, are not available Likewise, there is also no report of three- dimensional singularity ... divided into several sub-domains according to the singularity locations In each singular sub-domain, special functions that can account for the singularities are employed, whereas the standard methods...

Ngày tải lên: 15/09/2015, 21:09

... khác nh: palmatin, jatrorrhizin Giới hạn tạp chất palmatin không 2%, jatrorrhizin không % [6],[13] 1.1.3 Tính chất - Bột kết tinh màu vàng, không mùi, có vị đắng Tan nớc nóng, khó tan ethanol nớc ... dung dịch palmatin Khi thêm 2,5% palmatin, hàm lợng berberin bột nguyên liệu định lợng theo phơng pháp đo quang phổ hấp thụ UV-VIS DĐVN III tăng 1,14% Nếu có 2% palmatin nh giới hạn palmatin mà DĐVN ... II, p.1659 13 Pharmacopoeia of the peoples republic of China (2005), volum III, p.94- 96 38 14 The Korean Pharmacopoeia, 8th edition (2002), p.81-82 15 The Japanese Pharmacopoeia fifteenth edition...

Ngày tải lên: 07/09/2012, 14:57

... testing the real and imaginary parts after the phase has been calculated If both the real and imaginary parts are negative, subtract 180E (or B radians) from the calculated phase If the real part ... understand the characteristics of a frequency domain signal by looking at the real and imaginary parts In a typical program, the frequency domain signals are kept in rectangular notation until an observer ... written in equations as: Mag X [ ] , and the Phase of X [ ] , written as: Phase X [ ] The magnitude and phase are a pair-for-pair replacement for the real and imaginary parts For example, Mag X...

Ngày tải lên: 13/09/2012, 09:49

... domain aliasing In contrast, analog-to-digital conversion is an example of frequency domain aliasing A nonlinear action is taken in the time domain, that is, changing a continuous signal into a ... nonlinear phase All of the examples we have used so far are linear phase Figure 10-5b shows that nonlinear phase signals react to shifting in the same way In this example the nonlinear phase is a straight ... 10-6 Information contained in the phase Figure (a) shows a pulse-like waveform The signal in (b) is created by taking the DFT of (a) , replacing the phase with random numbers, and taking the Inverse...

Ngày tải lên: 13/09/2012, 09:49

... nothing more than a constant, while the phase is a straight line In comparison, the real and imaginary parts are sinusoidal oscillations that are difficult to attach a meaning to The second interesting ... 11-2 Delta function pairs in rectangular form Each sample in the time domain results in a cosine wave in the real part, and a negative sine wave in the imaginary part of the frequency domain (f) ... domain coincides with a sinc function squared (plus aliasing) in the frequency domain This transform pair isn't as important as the reason it is true A 2M & point triangle in the time domain can...

Ngày tải lên: 13/09/2012, 09:49

... point time domain signals into two N point frequency domain signals The two time domain signals are called the real part and the imaginary part, just as are the frequency domain signals In spite of ... domain signal into two N /2 % point frequency domain signals The time domain signal is called just that: the time domain signal The two signals in the frequency domain are called the real part and ... signal, point, sample, and value, refer to the combination of the real part and the imaginary part The FFT operates by decomposing an N point time domain signal into N time domain signals each composed...

Ngày tải lên: 13/09/2012, 09:50