lc ms analysis of hs like oligosaccharides

... highlight the importance of direct protein analysis of pathological samples Proteomic analysis undertakes providing a complete characterization of all of the species of proteins in the target ... involved in aspects of cell structure (cytoskeleton), of signaling, of metabolism or of transcription translation (Fig 5) Analysis of the FLS cellular proteome identified a number of proteins involved ... descriptions of the biological activity of the protein were subsequently retracted and the official designation of the protein is now a product of C19 ORF 10 [39] There are also suggestions of an IL-27...

Ngày tải lên: 09/08/2014, 01:23

... Differential expression profile of proteins common to both growth phases of O anthropi Differential expression profile of proteins common to both growth phases of O anthropi Fold changes of ≥1.5 or ≤0.5 ... genome sequence of B melitensis 16M, the closest relative of O anthropi, and assuming a high degree of synteny between the genomes of these organisms, we investigated the genomic context of each gene ... [35] due to the complex nature of the peptide mixtures to be analyzed, the separation capabilities of the liquid chromatography (LC) -mass spectrometry (MS) systems are often exceeded In this study...

Ngày tải lên: 14/08/2014, 07:21

... expression analysis of zfgfap-l Expression analysis of zfgfap-l by whole-mount in situ hybridization Expression analysis of zfgfap-l in mindbomb mutants (mib-/-) Functional analysis of zfgfap-l ... EXPRESSION ANALYSIS OF GFAP -LIKE GENE IN ZEBRAFISH EMBRYOGENESIS ANITA BALAKRISHNAN A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF ANATOMY NATIONAL UNIVERSITY OF SINGAPORE ... are a number of other factors (like redundancy of zfgfap-l) that may be involved 7 LIST OF TABLES Table 1: Summary of the principal events during Zebrafish embryogenesis 22 LIST OF FIGURES Fig...

Ngày tải lên: 05/10/2015, 22:31

... inhabitation of aquatic organisms The results of the measurement and the calculation of DVi, DIi and DI are shown in Table Three chemicals, terbucarb (MBPMC), bromobutide and tolclofos-methyl, ... toxicity tests were used as water quality indexes - 225 - GC /MS Analysis of Agricultural Chemicals GC /MS analysis was applied to 68 kinds of agricultural chemicals These compounds had been shown ... Europe (UNECE) (2003) Globally Harmonized System of Classification and Labelling of Chemicals (GHS), http://www.unece.org/trans/danger/publi/ghs/ghs_welcome_e.html (accessed May 2010) Wei D B., Kisuno...

Ngày tải lên: 05/09/2013, 10:15

... that eight different forms of the 55 residue tryptic peptide (unmodified and all possible forms with the above three PTMs) were observed during a nanoflow LC- MS ⁄ MS analysis of tryptic peptides from ... protein in peak II is a modified form of the 50S ribosomal protein, L34 All ions of A Fig Analysis of E coli ribosomal proteins by LC- MS, tandem (ETD ⁄ PTR) MS (A) Base peak ion chromatogram observed ... (Thermo Electron LTQ-Orbitrap, Thermo Scientific, 6274 Fig Analysis of recombinant Rev protein by LC- MS, tandem (ETD) MS (A) Average of three 300 ms ETD scans recorded on [M + 15H]+15 ions from Rev...

Ngày tải lên: 18/02/2014, 16:20

... University of Virginia, USA for the Procise sequencing results and for the MALDI-TOF MS analysis Ó FEBS 2002 Inhibition of snake venom myotoxins by DM64 (Eur J Biochem 269) 6061 REFERENCES Ohsaka, ... respectively, both of which are homodimeric proteins in native conditions The precise mode of action of class II PLA2 myotoxins remains elusive However, it seems clear that the initial target of these ... formation of Ôdelta lesionsÕ followed by hypercontraction of myofilaments due to increased intracellular levels of calcium ions [5,44] Despite the fact that myotoxic PLA2s affect a variety of cell...

Ngày tải lên: 21/02/2014, 01:21

... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some ... temperature of -5oC, and a narrow ID torch to maintain plasma stability Performance The ICP -MS sample introduction setup for the analysis of volatile organic solvents such as isopropyl alcohol (IPA) ... alternatives and, in the case of the sample uptake tubing, care must also be taken to avoid contamination of the sample through contact with the tubing material For routine analysis of organic solvents,...

Ngày tải lên: 05/03/2014, 21:20

... recombinant form of the human IGF1R by fusing the cDNA encoding the receptor ectodomain (residues 1±944) to the cDNA of the mouse Fc domain of immunoglobulin (encoding residues of the CH2 and CH3 ... Kinetic constants obtained from BIAcore analysis of binding of IGF-I, IGF-II and mutant IGF analogue to rhIGF1R Data were analysed using BIAevaluation software 3.0 and ®tted to a Langmuir : binding ... constant (Kd) was determined from the calculation of kd/ka, where ka is the association rate and kd is the dissociation rate Relative Kd is equal to Kd of IGF-I/Kd of IGF analogue Dashes indicate data...

Ngày tải lên: 08/03/2014, 22:20

... C-terminal side of Tyr) was cleaved in each case The same conclusion could be reached for the cleavage of these peptides if the Table Reverse-phase HPLC analysis of cleavage of the hexapeptide ... ´ J Marokhazi et al Substrate specificity of a serralysin -like enzyme Fig Cleavage site analysis of PrtA on oxidized insulin chain A (A) The position of cleavage sites (vertical arrows, a1–a3) ... positions, we took advantage of the apparent relaxed side-chain preference of PrtA to increase the solubility of the peptides (by choosing 1948 Fig Cleavage site analysis of PrtA on oxidized insulin...

Ngày tải lên: 23/03/2014, 09:20

... number of new gene clusters and to the discovery of nonclassical NRPS systems [2,5] The crystallographic structure of three members of the adenylate-forming enzyme family, firefly luciferase of Photinus ... average, shows that the average RMSD over the Ca of the entire structures is ˚ 2.6 A On the contrary, the average RMSD calculated ˚ over the Ca enclosed in a sphere of radius A centered at the GrsA ... the formation of a salt bridge between the a-amino group of the substrate and the carboxylic group of Asp235 and a hydrogen bond to the carbonyl atom of Ser330 The d-aldehyde group of the substrate...

Ngày tải lên: 23/03/2014, 13:20

... reason, in the last years, the development of new matrices have made UV-MALDI-TOF MS a suitable tool for 3810 2400 2800 Fig UV-MALDI-TOF MS analysis of the oligosaccharides released from C-terminal ... UV-MALDI-TOF MS analysis of the released oligosaccharides in the lineal positive mode using GA as matrix (A) oligosaccharides obtained from cruzipain, m ⁄ z range: 600–2474 Da (B) oligosaccharides ... Fig UV-MALDI-TOF MS analysis of the oligosaccharides released from cruzipain domain by PNGase F treatment in the linear negative-ion mode using nor-harmane as matrix (A) Analysis of the total...

Ngày tải lên: 23/03/2014, 15:20

... pulps of carious teeth than in those of healthy teeth, and the mean concentrations of Mg, Cd, Ba and Pb were higher in the pulps of carious teeth than in those of healthy teeth (Fig 3) Journal of ... spectrometer (ICP -MS) was used to measure the concentrations of trace elements ICP -MS (JMS-PLASMAX2, JOEL, Japan) was optimised by using a standard 89Y solution at a concentration of 10 ppb in 2% ... Optimisation 2.2 950 W Maximum ion intensity of 10 ppt of 89Y Collection and Preparation of Primary and Permanent Teeth A total of 64 primary teeth of all types (incisors, canines and molars)...

Ngày tải lên: 07/08/2014, 14:20

... OH OH Oglc(2-1)glc Oglc(2-1)glc(2-1)xyl Oglc(2-1)glc Oglc(2-1)glc(2-1)xyl Oglc(2-1)glc Oglc(2-1)glc Oglc(6-1)glc Oglc Oglc(2-1)glc Oglc(2-1)glc OH Oglc(2-1)glc OH OH Oglc(2-1)glc Oglc Oglc(2-1)xyl ... Oglc(2-1)xyl Oglc Oglc(2-1)rha Oglc Oglc(2-1)glc Oglc(2-1)glc Oxyl Oglc(2-1)rha Oglc OH H H H H H H H H H H OGlc(3-1)xyl H Oglc Oglc H Oglc(6-1)-glc Oglc Oglc Oglc Oglc(6-1)-glc' OH Oglc(6-1)glc Oglc(6-1)xyl ... Oglc(6-1)xyl OH OH Oglc Oglc(6-1)glc(6-1)xyl Oglc(6-1)glc Oglc(6-1)glc Oglc(6-1)xyl Oglc(6-1)araf Oglc Oglc Oglc OH OH – – – – – Figure Structures of the investigated saponins in P notoginseng glc, b-D-glucose;...

Ngày tải lên: 13/08/2014, 14:20

... comparability of gels, the densities of spots matched in all three gels were plotted and a correlation coefficient value was calculated The proteome of mDCs showed different levels of similarity ... digestion and MALDI-TOF (matrix-assisted laser desorption/ionisation-time of flight) mass spectrometric analysis; Table shows the corresponding protein data The factor of difference was calculated by ... functional relevance of differentially expressed proteins was confirmed by analysis of surface marker expression, cytokine profile, endocytic and T cell activation abilities of the cells, again...

Ngày tải lên: 14/08/2014, 18:20

... 0.540 ms 0.394 ms 0.397 ms 10.0.0.2 (10.0.0.2) 2.455 ms 2.479 ms 2.512 ms 10.0.0.3 (10.0.0.3) 4.812 ms 4.780 ms 4.747 ms 10.0.0.4 (10.0.0.4) 5.010 ms 4.903 ms 4.980 ms 10.0.0.5 (10.0.0.5) 5.520 ms ... 0.540 ms 0.394 ms 0.397 ms 10.0.0.2 (10.0.0.2) 2.455 ms 2.479 ms 2.512 ms 10.0.0.3 (10.0.0.3) 4.812 ms 4.780 ms 4.747 ms 10.0.0.4 (10.0.0.4) 5.010 ms 4.903 ms 4.980 ms 10.0.0.5 (10.0.0.5) 5.520 ms ... 0.540 ms 0.394 ms 0.397 ms 10.0.0.2 (10.0.0.2) 2.455 ms 2.479 ms 2.512 ms 10.0.0.3 (10.0.0.3) 4.812 ms 4.780 ms 4.747 ms 10.0.0.4 (10.0.0.4) 5.010 ms 4.903 ms 4.980 ms 10.0.0.5 (10.0.0.5) 5.520 ms...

Ngày tải lên: 18/10/2014, 19:13

... patterns of acidic chitinase isoforms These zymograms revealed that leaves, roots and crowns (lanes through 3) contained a predominant isoform, designated as Chi3A, plus two to four minor isoforms ... protein complexes, which consist of various combinations of a chitinase, two glucanase -like proteins (GLPs) and a thaumatin -like protein (TLP) Western blot analysis of the strawberry chitinase complex, ... consisting of multiple polypeptides Western blot analysis revealed that all these complexes are composed of various combinations of one 35-kDa chitinase -like protein (CLP), two β1,3-glucanase-like...

Ngày tải lên: 13/11/2014, 09:25

... shrinkage calculation is numerically extended to cover more exactly “creep of creep” effects ( it is possible to decrease number of time steps ) Extensions of British Standard live load calculation ... rmbin04.rm8) into the new database " The full calculation will be made on selection of $RECALC 3.113.2 Recalc Window The user is able to control the fibre stress calculation with three check boxes • Composite ... DoList/Influence file New possibility of aero class definition added: couple of constants (factor Cd and its derivation Cd’) for all directions Additional option for calculation of cross section shear area...

Ngày tải lên: 06/09/2012, 15:18

... Ownership of the program and the documentation remain with TDV Austria Use of the program and the documentation is restricted to the licensed users Unlicensed use of the program or reproduction of the ... RM2000/GP2000 Creep & Shrinkage Calculation using RM2000 Procedure Guide Loading Case 16 CONSTRUCTION SCHEDULE Loads Lcase Stage Calculation Action Action Calculation Action Creep © TDV – Technische ... Select Calculation Action Select CREEP Inp2: Number of Time Steps (e.g:.1) Out1: Loading case Number (e.g.: 601) Out2: List-File Delta-T (Day): length of Creeping Time since last creep calculation...

Ngày tải lên: 06/09/2012, 15:55

... of loading and construction stages "RECALC Definition of calculation parameters and start of the calculation "RESULTS Viewing of results and creating of output files (plots and listings) Note: ... General Calculation of basic data 8-43 Evaluation of stresses due to service and ultimate load 8-46 Calculation of reinforcement to take tensile forces 8-50 Preparation of the ... selection of the only subfunction !RECALC, the calculation will be started On selection of "RESULTS, the following sub-functions list will be displayed on the right hand side of the screen #LCASE...

Ngày tải lên: 06/09/2012, 15:55

... Services, LLC Role of the sponsor SM, AQ, and NK of Johnson and Johnson met authorship criteria by participating in the study design, analysis of results, interpretation of findings, and drafting of ... because of activity against key pathogens Consideration of the influence of appropriateness of empiric therapy against subsequently identified pathogens was beyond the scope of this economic analysis ... Independent statistical analysis The accuracy of the data analysis was independently verified by William Shannon, PhD, Associate Professor of Biostatistics in Medicine, Department of Medicine, Washington...

Ngày tải lên: 25/10/2012, 10:02