ladder and functional block programming

... is "1" at inputs I0.0 and I0.1 and I0.2 • And there is a negative edge at input I0.3 • And the signal state is "1" at input I0.4 Ladder Logic (LAD) for S7-300 and S7-400 Programming A5E00261407-01 ... And there is a positive edge at input I0.3 • 1-20 The signal state is "1" at inputs I0.0 and I0.1 and I0.2 And the signal state is "1" at input I0.4 Ladder Logic (LAD) for S7-300 and S7-400 Programming ... S7-300/400 Provides reference information 6ES7810-4CA07-8BW1 and describes the programming languages LAD, FBD and STL, and standard and system function extending the scope of the STEP basic information...

Ngày tải lên: 22/03/2014, 23:20

... digits and are called binary digits or bits In conjunction with AND, OR, XOR and outputs, a stands for logical YES and a for logical NO The bit logic instructions interpret signal states of and and ... information • Ladder Logic (LAD)/Function Block Diagram (FBD)/Statement List (STL) for S7-300/400 manuals • Standard and System Functions for S7-300/400 Volume and Volume iv Part of the STEP Standard ... Converter Manual STEP Reference with Provides reference information and describes the programming languages LAD, FBD, and STL, and standard and system functions extending the scope of the STEP basic information...

Ngày tải lên: 08/08/2014, 01:22

... A Kozlowski and Wanda Sliwa 2010 ISBN: 978-1-60876-709-0 Silicon-Organic Oligomers and Polymers with Inorganic and Organic-Inorganic Main Chains Nodar Lekishvili, Victor Kopylov and Gennady Zaikov ... Stabilizers and Amphiphilic Block Copolymers Liudvikas Segewicz and Marijus Petrowsky (Editors) 2010 ISBN: 978-1-60692-928-5 POLYMER SCIENCE AND TECHNOLOGY SERIES POLYMER AGING, STABILIZERS AND AMPHIPHILIC ... self-assembly of block copolymers and recent developments in the field of various amphiphilic block copolymers, and future perspectives in the field of DHBCs regarding general polymer science and nanotechnology...

Ngày tải lên: 12/02/2014, 16:20

... lower extremities were carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from chair without using arms and shoulder external rotation (full) A younger ... injury or an illness Best performance was in "standing from chair without using arms" (84%), semi tandem stand was completed by 78% and full tandem stand by 72% The poorest performance was in shoulder ... in Myanmar, 84% in Indonesia and 62% in Thailand (10) It was D N Fernando and R de A Seneviratna 14 Table Self-assessed "health status" by some psychosocial factors and indicators of use of health...

Ngày tải lên: 14/02/2014, 06:20

... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural Genomics ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively...

Ngày tải lên: 15/02/2014, 01:20

... (a helix signature), 218 nm (b strand signature), 202–204 nm (random coil signature) In WhiB5 and WhiB6, the structure was dominated by a helices and b strands and the proportion of these structural ... refolding were  98% pure and were brownish (Figs S1 and S2) The presence of four conserved cysteines and the brownish appearance of purified WhiB1, WhiB2, WhiB5, WhiB6 and WhiB7 indicated the presence ... WhiB6 and WhiB7 the loss was  8–10% After 48 h of air exposure, losses were as follows:  80% in WhiB1,  75% in WhiB2 and WhiB5,  65% in WhiB3,  60% in WhiB4, and  35–40% in WhiB6 and WhiB7...

Ngày tải lên: 18/02/2014, 12:20

... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three 310-helices The core of the protein is made up of a nine-stranded b-sheet flanked by a1, a5 and ... are represented by pink, cyan, blue and yellow, respectively The tetrameric interactions are between the subunits A and C, and B and D, by way of the b hairpins and the loop, which connects b2 to...

Ngày tải lên: 18/02/2014, 14:20

... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands) ... intermediate levels in heart and placenta, and the lowest levels in spleen, kidney, liver and lung The transcript was not detectable in brain, thymus, muscles, small intestine and colon (Fig 3A) The ... within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and MCPIP (bottom panel)...

Ngày tải lên: 18/02/2014, 14:20

... performed with rabbit preimmune and post-third inoculation sera against a M tuberculosis sonicate (lanes 1, and 5) and a membrane fraction (lanes and 4) Lanes and 3, absence of recognition of ... nm) (Fig 5A) Scatchard and Hill analyses were then performed and dissociation constants (Kd) were calculated for Rv2560 HABP 11024 for U937 and A549 cell lines (Kd of 380 and 800 nm, respectively) ... for U937 and 1.2 for A549 cells, suggesting positive ligand–receptor cooperativity The numbers of binding sites per cell calculated for peptide 11024 were 200 000 and 000 000 for U937 and A549...

Ngày tải lên: 18/02/2014, 16:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... water molecule fixing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and E A phosphate ion was found in the donor site ... Ala The mutant enzymes, F133N and F133A, were expressed, purified and characterized With mm UMP and ATP as substrates, the activities of F133N and F133A were only 50 and 20% of that of the wild-type...

Ngày tải lên: 18/02/2014, 16:20

... 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... prim–pol domain S Prato et al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in ... known to serve as ligands for divalent cations and as tags revealing the presence of DNA polymerases in the active site [18–20] In this study, we report on the structural and functional characterization...

Ngày tải lên: 18/02/2014, 18:20

... (accession numbers AJ880690 and AJ880691 for cDNAs, Q53I65 and Q53I64 for amino-acid sequences) were aligned and compared with other globins in the multiple sequence alignment (Fig and Table 1) The sequence ... typical molecular features: Lys-A9, Trp-B10 and also a deletion of three residues between the A and B helices, and a deletion of one residue between the F and G helices Moreover, the two residues ... Asp-A4, Trp-A16, Phe-B10 and LeuB13 Furthermore, it shows an insertion of three residues between the A and B helices, an insertion of one residue between the F and G helices, and a three-residue motif...

Ngày tải lên: 19/02/2014, 02:20

... glucose and hemin chloride (13 lgÆmL)1), and further incubated at 30 °C for 24 h Then, the cells were collected and suspended in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed ... cDNA and functional analysis of several other EST clones belonging to this subfamily is in progress On the other hand, sterol oxygenases thus far identified not show high sequence homology and ... vector MS fragmentations of entries A and B are shown in the lower panel CYP93E1 has 24-hydroxylase activities for both b-amyrin and sophoradiol substrates b-amyrin and sophoradiol hydroxylase activities...

Ngày tải lên: 19/02/2014, 07:20

... chromosomes; PDGF-a and -b on chromosomes and 22 [17,18], and PDGF-c and -d on chromosomes and 11 [19], respectively The genomic organization of the pdgf genes is quite similar, although PDGF-c and -d genes ... exons in PDGF-c (exons and 6) and PDGF-d (exons and 7) encoding the cystine knot motifs resemble the corresponding exons in the PDGF-a and -b (exons and 5) genes Both in PDGF-a and -b, exon encodes ... While PDGF-A and -B can form both homo- and heterodimers (PDGF-AA, -AB, -BB), PDGF-C and -D exist only as homodimers (PDGF-CC and -DD) The full-length PDGF-C and -D monomers are 54–55 kDa and 49–56...

Ngày tải lên: 19/02/2014, 07:20

... concentrations: 40 and 80 mM (lanes and 3) for h MTs were also treated for the same period with a reducing agent (dithiothreitol) at 40 and 80 mM (lanes and 5) To reduce aggregation, MT samples were handled ... urchin, snail and oyster) have so far been elucidated [11–17] As in mammals and fish [18], echinoderm MTs contain two globular domains binding four and three bivalent ions [19] On the other hand, in ... Vergani et al right-handed b domain binds three bivalent metal ions The C-terminal a domain is left-handed and binds four bivalent ions Zinc is preferentially located in the b domain and cadmium in...

Ngày tải lên: 19/02/2014, 07:20

... in Asian and Mediterranean countries [15] This paper describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular ... characterization Materials and methods Materials Plasmids pGEM-3Zf(+) and pQE30 were from Promega and QIAGEN, respectively [32P]dCTP[aP] (3000 CiÆ mmol)1) was from DuPont-NEN Restriction enzymes and Taq DNA ... as all enzymes showed only one band, except those which cut once into the gene sequence (BamHI, KpnI and SalI), which exhibited two hybridizing bands Chromosomal bands from L donovani 1S2D strain...

Ngày tải lên: 21/02/2014, 00:20

... Asn117, Tyr287 and Arg311), and the two Cys residues (105 and 186) that form a disulfide bond between EL1 and EL2 to maintain the receptor in a high affinity conformational state Several Ser and Thr residues ... positions (3 and 10), while xTRHR2 had these sites at positions and 12 The glycosylation site in EL2 (Asn167 for xTRHR1 and Asn172 for xTRHR2) and the two homologous Cys residues (335 and 337 for ... xTRHR2 and four xTRHR3), and stomach (14 clones: nine xTRHR1, three xTRHR2 and two xTRHR3) Only xTRHR1 was present in the lung (11 clones tested), the intestine (three clones) and the urinary bladder...

Ngày tải lên: 21/02/2014, 03:20

... in green, and the cluster of hydrophobic residues Trp390, Trp393, and Trp394 is in red The catalytic site is enlarged in B and illustrates the conserved disulfide bridge between Cys239 and Cys216 ... gradient (broken line), and concentrations of LPL (j) and lipase activities (h) were measured in each fraction as described in the Materials and methods The upper, middle, and lower panels are the ... sequence containing Trp393 and Trp394 Fig Heparin binding of wild-type LPL and the WW mutant Wild-type LPL (left) and WW mutant (right) were applied to heparinSuperose FPLC and eluted by a linear...

Ngày tải lên: 21/02/2014, 15:20

... University Ghent, Belgium Fandi Ibrahim Health and Nutrition Danisco Kantvik, Finland and Functional Foods Forum University of Turku Turku, Finland Su Jin Danisco China Ltd Shanghai and Beijing, People’s ... Institute of Public Health and Clinical Nutrition University of Eastern Finland Kuopio, Finland Sampo J Lahtinen Health and Nutrition Danisco Kantvik, Finland xvi ◾ Contributors Andrea Lauková Institute ... a new strand while displacing the plus-strand (Figure 2.1) When a cycle has been completed, a double-stranded plasmid and a circular single-stranded intermediate is formed by cutting and religating...

Ngày tải lên: 26/02/2014, 03:20

... be distinguished Helices B, E, F and H, arranged in an up -and- down topology, form the structural core, whereas the short helices C, D and G on one side, and A, I and J on the other, represent types ... glutamate and aspartate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J In ... b-sheet flanked by a-helices Five b-strands, labelled from A to E, run all contiguous The N-terminus of the monomer includes strand A, an a-helix and a long stretch, and the C-terminal portion includes...

Ngày tải lên: 06/03/2014, 00:21