lacz apos gene carried by the phage vector

... reported by Noe and colleagues [10] To explain all these effects, the authors suggested the presence of a signal transduction pathway induced either directly by sE-cad or indirectly by the disruption ... [50] However, the site at which the PI3K signaling feeds into the Erk activating pathway varies in these systems: at the Ras, Raf or MEK activation level In the Decma-induced pathway, the signal ... treatment, neither treatment with the E-cad2 antibody nor the EGFR antibody induced Erk activation (Fig 2E) Taken together, these results suggest that the observed Erk activation was specific for the disruption...

Ngày tải lên: 19/02/2014, 05:20

... modulation of the transcription rates of all genes, accounting for the global regulation of gene expression [22] Gene- specific cis-regulatory elements that mediate rhythmic gene expression might therefore ... changes in the status of the chromosome These changes in chromosomal status influence the transcriptional activity of all promoters (including kai promoters) in the chromosome so that there are ... gene expression; the majority of genes have significant basal activity and are rhythmically modulated by the KaiABC oscillator, while in a smaller subset of genes, the oscillator dominates transcriptional...

Ngày tải lên: 09/08/2014, 20:20

... truyền phage T4 cho thấy gene phage T4 tạo cụm mở rộng theo chức chúng Chẳng hạn có cụm lớn gene dùng cho chép DNA vị trí phần tư bên phía phải có cụm gene tổng hợp cấu phần tạo nên đầu phage ... cụm gene tổng hợp cấu phần tạo nên đầu phage phía vòng tròn Phân tử DNA phage T4 phân tử sợi đơn dạng thẳng, đầu tận DNA phage T4 nhân lên lặp đoạn đầu cuối (terminal redundant) Do vậy, phân tử ... tái tổ hợp gen phage T4 xảy thường xuyên, trung bình có khoảng 20% kiện tái tổ hợp xảy nhiễm sắc thể Khi phân tử DNA gói vào phần đầu, cắt enzyme chứa khoảng 102% chiều dài gen phage T4, có chứa...

Ngày tải lên: 20/10/2013, 14:15

... that the wide-scale profiling of intracellular signals leading to ERK1/2 activation may have not been entirely encompassed by the drug-treatment Such a hypothesis is further confirmed by the observation ... signaling and that the activation of ERK by GPCRs closely parallels that employed by RTK [33] On the whole, these observations suggest that the inhibitory effect elicited by NAMI-A on ERK signaling ... elicited by NAMI-A may be a part of the molecular mechanism by which this drug exerts its antimetastatic activity in vivo Such a view may be further inferred by the following observations The NAMI-induced...

Ngày tải lên: 21/02/2014, 01:21

... and the 5¢-termini of the NtFAD3 transgene cDNAs were distributed in the same region of the transgene sequences, suggesting that the NtFAD3 transgene was transcribed under the control of the ... activity of the transgene When transcription of the transgene was partially repressed by methylation of transgene promoter sequences, siRNAs targeting the coding region of the transgene disappeared ... dense methylation of the promoter sequences, and siRNAs for the nptII gene disappear When the expression of the transgene is almost completely inhibited by DNA methylation, the generation of siRNAs...

Ngày tải lên: 22/03/2014, 21:20

... kbp upstream of the embryonic e-globin gene The murine b-globin gene locus, which is depicted on the bottom, consists of four genes which are expressed either in erythroid cells of the embryonic ... to the b-globin gene promoter [20] At the same time, we observed a reduction in the recruitment of CBP and p300 to these sites To examine in more detail whether USF recruits coactivators to the ... during the differentiation of erythroid cells Observations that the LCR recruits transcription complexes before they become detectable at globin gene promoters support the hypothesis that the LCR...

Ngày tải lên: 23/03/2014, 07:20

... anisotropy of the ligand field to the heme iron in Syn HO-2 is relatively large The seating of the heme in the heme pocket might be less homogeneous than in Syn HO-1 As the pH of the solution rises ... nm increased These results indicate that the iron(III) form of myoglobin was reduced to the iron(II) form by the NADPH ⁄ FNR ⁄ Fd system and this was followed by CO binding to yield the iron(II)-CO ... substitute for O2 and electrons in the conversion of hemin to verdoheme, thereby implying that the chemistry of heme degradation by Syn HO-2 is similar to that by other HOs Accordingly, we conclude...

Ngày tải lên: 30/03/2014, 15:20

... activities, we examined the effect of this spice principle on the synthesis of MIP-2 by astrocytes Our results indicate that curcumin potently inhibits MIP-2 production at the level of gene http://www.jneuroinflammation.com/content/2/1/8 ... WI) The direction of the insert was confirmed by restriction endonuclease digestion and its fidelity determined by sequence analyses as previously described[15] The MIP-2 promoter-reporter gene ... http://www.jneuroinflammation.com/content/2/1/8 Figure transcription Curcumin inhibits the activity of MIP-2 at the level of gene Curcumin inhibits the activity of MIP-2 at the level of gene transcription Confluent astrocyte cultures...

Ngày tải lên: 19/06/2014, 22:20

... followed by 25 cycles of the program: 98°C (3 s); 65°C (5 s); 72°C (20 s) and a final extension at 72°C (1 min) The amplicon was cloned into TA vector to generate the recombinant plasmid pHR1 Further, ... μmax of the recombinant strain could also be attributed to the metabolic load imposed by the recombinant plasmid on the host (Bentley et al 1990) Further, metabolic flux analysis needs to be carried ... reuteri Further engineering of the strain to improve the redox balance and minimize the formation of byproducts like lactate and ethanol could pave the way for maximizing 1, 3-PD biosynthesis Acknowledgements...

Ngày tải lên: 20/06/2014, 23:20

... classified into 1 -vector and 0 -vector signals The input signals X k are 1 -vector if they satisfy U(W T X k ) = i i They are 0 -vector if they satisfy U(W T X k ) = In vector i space, these two classes ... are separated by an optimal hyperplane, which is bounded by W T X m ≥ and W T X m+1 < 0, i i when the output value is m Hence, the vector X m is called the i 1-support vector and the vector X m+1 ... samples such that all the points with the same class are on the same sides of the hyperplane Let wo and bo denote the optimum values of the weight vector and bias, respectively The optimal separating...

Ngày tải lên: 22/06/2014, 23:20

... gene phage T4 tạo cụm mở rộng theo chức chúng Chẳng hạn có cụm lớn gene dùng cho chép DNA vị trí phần tư bên phía phải có cụm gene tổng hợp cấu phần tạo nên đầu phage phía vòng ... Tần số tái tổ hợp = (Số phage tổ hợp/Tổng số phage) x100 Sự hình thành vết tan thể đột biến phage Phage phát dễ dàng chu trình tan, tế bào bị nhiễm phage vỡ giải phóng hạt phage vào môi trường ... hai chứa gene gag gene pol Retrovirus có gene mã hóa cho protein trình chép: gene gag mã hóa cho protein có vai trò làm biến tính RNA genome Gene pol mã hóa enzyme reverse transcriptase Gene env...

Ngày tải lên: 13/07/2014, 09:20

... deletions The last common ancestor (LCA) of the full set had a minimum of three genes, the LCA of the proteobacteria had four genes, while the LCA of the α/β-proteobacteria had six genes The tree ... least within the proteobacteria (Figure 2), in this case with four other gene families, defined by the E coli yfaA, yfaQ, yfaP and yfaT genes All these genes have signal sequences and their encoded ... well illustrated by the two species of Helicobacter, one exploiting the acidic stomach and the other the very different environment of the liver: only the latter has a bact-α2M The H hepaticus...

Ngày tải lên: 09/08/2014, 20:20

... of the enhanced green fluorescent protein (EGFP) The obtained lentiviral constructs were confirmed by PCR reaction, enzyme digestion and gene sequencing To generate lentivirus, the expression vectors ... [33,34] Further detailed studies are required to elucidate whether it may also function as a master controller in modulating genetic networks within the HSC system Another discovery is the finding ... granulocytic differentiation The results obtained here were similar as compared with the Sall4 activities in the human system [19] We have hypothesized that the Sall4 gene is also involved in mediating...

Ngày tải lên: 10/08/2014, 21:23

... turn secretes TGF-β1, further augmenting the autocrine regulating cycle Another involved pathway is the activation of Smad cascade The Col 1A2 gene expression is induced via the phosphorylation of ... performed the EMSA and edited the manuscript EL coordinated the study and finally edited the manuscript All authors have read and approved the content of the manuscript described in the User Bulletin ... inducible on the 7th day (Fig 1E) The results are basically similar to that described by Herweijer et al who showed time course of gene expression after plasmid DNA gene transfer to the liver:...

Ngày tải lên: 13/08/2014, 13:20

... synchrony The data should speak for themselves 7) The DNA distribution of cells should be narrow in the synchronized cells and these distributions should then reflect the movement of cells through the ... performed but the cells are all recombined together and analyzed These combined cells should not give a variable pattern of gene expression This controls for perturbation of the culture by the selection ... that the cells are not synchronized The DNA patterns presented by Whitfield et al testify to the fact that the cells are not only not synchronized but are also perturbed The DNA patterns in the...

Ngày tải lên: 13/08/2014, 23:20

... transcription of ZDS1 by controlling the local subunit composition of RNA Pol II On the other hand, Nhp6p generally supports transcription elongation, as suggested by the 6-AU phenotype of the NHP6 deletion ... arginine to cause the degradation of the free RUra3p reporter by the enzymes of the N-end rule The URA3 gene encodes orotidine-5'-phosphate decarboxylase This enzyme is required for biosynthesis of uracil ... required for gene transcription are part of gene expression machines (GEMs) (Maniatis and Reed, 2002) in the nuclear periphery and uninduced genes are located in the centre of the nucleus Upon gene induction,...

Ngày tải lên: 11/09/2015, 16:08

... treatment for these difficult diseases 1.1.1 Gene Therapy The main concept of gene therapy is to use genetic materials to replace or restore function of the missing, abnormal or malfunctioning gene components, ... is another famous gene delivery vector The first generation of replication-defective Ad vectors proved to have limited use in gene therapy, mainly due to a strong host immune response to the viral ... powerful vector that is useful for various types of gene therapies On the other hand, baculoviruses are non-replicative in mammalian cells; it makes the gene deliveries mediated by baculovirus vectors...

Ngày tải lên: 14/09/2015, 11:28

... specificity of the therapeutic gene is primarily determined by the activity of the promoter at the transcriptional level, and the duration of the transgene expression can also be influenced by the selection ... of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another key Chapter Introduction issue of gene therapy The tropism of the vector can ... AAV vector 15 Chapter Introduction generated for gene delivery, the Rep and Cap genes are replaced by the therapeutic genes AAV is a commonly used viral vector, especially for gene delivery to the...

Ngày tải lên: 16/09/2015, 08:30

... distributed into the six MSL systems by water pumps (Fig 1b) River water discharged into the MSL systems was moved down gravitationally and treated water was returned to the lower position than the water ... properties: pH by glass electrode method, ORP, BOD and suspended solids (SS) by Standard Method for the Examination of Water and Wastewater (1995), COD by potassium dichromate method, NH4-N by Nessler ... addition to above regular sampling for the determination of the seasonal fluctuation of river water and treated water, we determined the daily fluctuation of them by those sampling at hour interval...

Ngày tải lên: 05/09/2013, 08:40