kinetical thermodynamical and analytical aspects of macromolecular metal complex formation
Handbook of Polymer Synthesis Second Edition Episode 11 pptx
Ngày tải lên :
13/08/2014, 16:21
... Kinetical, Thermodynamical, and Analytical Aspects of Macromolecular Metal Complex Formation As in low molecular weight metal complexes, the process of complex formation of metal ion binding in macromolecular ... The ligand of a metal complex or metal chelate is part of a linear or crosslinked macromolecule (Figure 2) Either a multifunctional ligand /metal complex or a multifunctional ligand metal complex ... strength of the formed bonds between metal and ligand depend on the ionisation potential of the metal ion, its electron affinity and the donor properties of the ligand groups For macromolecular metal complexes...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
Ngày tải lên :
17/03/2014, 03:20
... protein and the 4-kDa peptide/43-kDa protein complex (A) Chromatogram of the 43-kDa protein (B) Chromatogram of the 4-kDa peptide and the 43-kDa protein complex The elution points of the size-standard ... fragment and fragment in the 4-kDa peptide tertiary structure, and comparison of the tertiary structure of insulin and the 4-kDa peptide (A and B) Fragment and fragment are shown in orange and red, ... topology of side-chains of putative active residues in both insulin and the 4-kDa peptide (E and F) and b-strand, and fragment contains hairpin-b [14] These structures form the sheet of the putative...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
Ngày tải lên :
23/03/2014, 12:20
... observed two dominant isoelectric points of 7.7 and 7.2 and two minor points at 7.4 and 7.1 (Fig 1C) Analysis of p95 revealed isoelectric points of 7.7, 7.2 and 7.1 (Fig 1C) These points were located ... folding of proteins or inappropriate assembly of multimeric protein complexes can influence the surface and overall expression of these proteins in cells and can also alter the half-life of the ... the membrane of each Ba/F3 cell was determined by FACS and is reported as mean fluorescence intensity (Fig 3C shows values of each of the clones) The relative enzyme activity of each of the mutants...
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báo cáo hóa học:" Unsaturated phosphatidylcholines lining on the surface of cartilage and its possible physiological roles" potx
Ngày tải lên :
20/06/2014, 00:20
... µl of individual synthetic PC (PLPC, POPC and DPPC) chloroform solution at a concentration of 21.85 mg/ml was deposited on each side of a disc of white nylon filter paper with a pore diameter of ... compare the three pairs, PLPC and DPPC, POPC and DPPC, and PLPC and POPC, separately There were significant differences between POPC and DPPC (p = 0.002), and PLPC and POPC (p = 0.003) There was ... conception and design, the conduction of the experiment, the collection, analysis and interpretation of data, the drafting the manuscript and acquisition of funding RWC contributes to the analysis and...
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Báo cáo toán học: " Spin-related tunneling through a nanostructured electric-magnetic barrier on the surface of a topological insulator" potx
Ngày tải lên :
20/06/2014, 20:20
... spin of the charge carriers, while for graphene it stands for the pseudo spin, i.e., the A and B sublattices of graphene Hence, it is natural to manipulate spin transport on the surface of a ... the reversal of the momentum px Interestingly, the gate voltage can be used to control the reflection and transmission, and therefore tune the spin polarization of the reflected and transmitted ... S, Heinzel T, Schulz N: Effect of edge transmission and elastic scattering on the resistance of magnetic barriers: Experiment and theory Phys Rev B 2007, 75:035341 and references therein [19] Ion...
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Báo cáo lâm nghiệp: "Interactions of ozone and pathogens on the surface structure of Norway spruce needle" potx
Ngày tải lên :
09/08/2014, 04:20
... edges of the stomata (P= 0.0346) The apparently newly crystallized small wax tubes covering the eroded area were typical of this type of injury (Fig 1A and B) - Apart from the erosion of wax ... S-compounds are known to alter the structure of epicuticular waxes of conifers (Cape and Fowler, 1981; Huttunen and Laine, 1981; 1983; Cape, 1983; Crossley and Fowler, 1986; Schmitt et al., 1987) ... causes chemical and morphological changes in the epicuticular waxes of the needles (Huttunen and Laine, 1983; Gunthardt-Goerg, 1986) Erosion of the epicuticular wax, both natural and that caused...
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Báo cáo y học: "Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture" doc
Ngày tải lên :
09/08/2014, 10:20
... implants were removed as a result of clinical infection and five as a result of aseptic loosening of the prosthesis On removal, the femoral and acetabular cup components of the prosthetic hip joint ... analysis with RsaI and MnlI About 0.5 μg of each PCR product was digested at 37°C in a total volume of 15 μl with 2.0 U of MnlI (Helena Biosciences, Sunderland, UK) or 2.0 U of RsaI (Promega) ... A, Drancourt M, Raoult D: Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections J Clin Microbiol...
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Báo cáo y học: " HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses" pot
Ngày tải lên :
11/08/2014, 08:21
... such as 17b and 48d The MAbs 17b and 48d recognize a cluster of gp120 epitopes that are centered on the β 19 strand and partially overlap the co-receptor binding site [13,14] While many of the known ... Monoclonal antibodies 4E10 and 447-52D and soluble CD4 (sCD4) [19] Two human MAbs (4E10 and 447-52D) and sCD4 were kindly gifted from the National Institute for Biological Standards and Control (NIBSC, ... level of virus control was subtracted with p24 level of virus containing each dilution of MAb or sCD4 before being divided by p24 level of virus control and then multiplied by 100 The toxicity of...
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Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
Ngày tải lên :
03/11/2012, 09:57
... 0) and on days 3, 7, and 14 for PCR studies and days 0, 1, and for measurement of soluble HLA-DR Blood (7.5 ml) was collected in preservative free heparin (final concentration of 100 iu/ml) and ... percentage of cells expressing HLADR on monocytes and the density of HLA-DR was measured Immuno-fluorescent microscopy of HLA-DR in PBMC Cytospins of PBMC obtained from healthy controls and septic ... equal volume of Ficoll and centrifuged at 400xg for 20 minutes at 18˚C Following isolation and washing of the PBMC viability was assessed to greater than 95% using trypan blue exclusion and the PBMC...
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Effect of urban emissions on the horizontal distribution of metal concentration in sediments in the vicinity of Asian large cities
Ngày tải lên :
05/09/2013, 09:08
... wastewater and urban storm water runoff, contained high concentrations of lead and zinc Table summarizes the results of all the target metals in this study Concentration of metals like copper and nickel ... contained 64% of sand and other upstream samples mostly consisted more than 97% of sand Lead and zinc concentrations in Figure show higher concentrations for fine sediments in the downstream and smaller ... mgPb/kg and 110 mgZn/kg based on the uniform distribution of these metals in the sediments of the lake The higher concentration in the northwest part of the lake is probably due to reverse flow of...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
Ngày tải lên :
19/02/2014, 17:20
... similar with respect to chemical shifts of the signals in intermediate stages of oxidation, and that formation of the complex does not lead to a marked decrease of the spectral quality in the experimental ... state of the protein and have standard errors < meV The off-diagonal elements represent the redox- and redox–Bohr interactions between the centres All parameters are reported in units of meV, ... visible absorption measurements, and the possibility of interference from the redox mediators on complex formation Therefore, haem I and the interaction between haems I and IV were chosen as references...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
Ngày tải lên :
21/02/2014, 03:20
... In¯uence of the addition of imidazole on the peroxidase activity of Fe(ToCPP), a,a-1,2- and -a,b-1,2-Fe(DoCPP) and their complexes with 13G10 Variations of the initial rate of oxidation of 0.2 ... 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding of H2O2 and imidazole on the iron of 13G10-(Fe(ToCPP), (D) binding of H2O2 and imidazole on the iron of 13G10-a,a-1,2-Fe(DoCPP) ... increasing concentrations of imidazole and compared to that of the a,a- and a,b-1,2-Fe(DoCPP))13G10 complexes With both a,b -and a,a-1,2-Fe(DoCPP), the rate of oxidation of ABTS by H2O2 increased...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
Ngày tải lên :
06/03/2014, 11:20
... loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local artificial library design in (A) CDR loops of Fv and (B) BC, DE and FG ... between Thr25 and Glu26 Fv Fig Functionalization of small scaffold proteins by replacing a loop of the scaffold protein with a CDR loop of antibody fragments (A) Replacement of the candidate location ... interleukin-6, CD40L, and CD28 Conclusions and outlook Accurate structural descriptions of protein–protein complexes provide support for the replacement of binding site sequences and thus binding...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
Ngày tải lên :
07/03/2014, 09:20
... occurrence of the metal ions correlates with the conformation of the flap subdomain (132–174) and the ‘binding’ of adjacent STP monomers over the active sites of monomers C and D, but not of A and B ... HsSTP and SaSTP monomers active sites In the alignment metal ions M1 and M2, Wn, and carboxylate groups of Asp36, Asp192 and Asp231 were used Alignment of 12 atoms resulted in an rmsd value of ˚ ... the question of whether the difference in conformation of the flap domain is related to binding of the third metal ion or the substrate The ligands for Mn3 in MtSTP were Asp118, Asp191 and Ser160,...
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Báo cáo khoa học: A kinetic approach to the dependence of dissimilatory metal reduction by Shewanella oneidensis MR-1 on the outer membrane cytochromes c OmcA and OmcB potx
Ngày tải lên :
07/03/2014, 09:20
... absence of OmcA results in metal reduction rates that are 55% and 62% of those of the MR-1 parent strain for Mn(IV) and V(V), respectively [10] Purified and dithionite-reduced preparations of both ... characterized by IC50s of 10.7 lm and 81.4 lm for inhibition of OmcA and OmcB, respectively Modes of inhibition of either OmcA or OmcB by V(V) The modes of inhibition of either OmcA- or OmcBdependent ... the different metals are shown in Fig 1B Construction of the omcA– and omcB– single mutants and of the omcA– omcB– double mutant strains of MR-1 Single omcA– and omcB– mutants and a double omcA–...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
Ngày tải lên :
07/03/2014, 12:20
... 1.25 mL of 1.5 m TrisHCl buffer, pH 8.8, and mL of water was boiled for and after cooling to room temperature, 1.65 mL of acrylamide solution (30%), 60 lL of 10% ammonium persulfate solution and ... Superposition of the A avamori and A niger structures with those of S fibuligera glucoamylase complexes with Tris and acarbose shows that the corresponding residues are Glu210, general acid and Glu456, ... omitted Two types of gels were used: (1) Standard 10% polyacrylamide gel: 1.25 mL of 1.5 m TrisHCl buffer, pH 8.8, 1.45 mL of water, 2.2 mL of acrylamide solution (30%), 60 lL of 10% ammonium persulfate...
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Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
Ngày tải lên :
08/03/2014, 09:20
... 250 lg of POPC and lg of I tartaricus ATP synthase were diluted into 300 lL of 50 mM potassium phosphate, pH 7.0, mM MgCl2 and 100 mM K2SO4 and used for a single measurement For titration of uorescence ... localization of 1.3 2.4 A and 1.8 2.8 A from the center of the bilayer was found for the ATP synthases of I tartaricus and E coli, respectively These data correspond very well with a distance of 18 ... quenching of ACMA uorescence was monitored with a RF-5001PC spectrouorometer (Shimadzu) using excitation and emission wavelengths of 410 and 480 nm, respectively ể FEBS 2002 Localization of ion...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
Ngày tải lên :
16/03/2014, 01:20
... role of HSP70 in the inhibition of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP-binding domain of HSP70 in the prevention of the release of ... domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 ... etoposide and glucocorticoids [10,11] Smac promotes caspase activation by eliminating inhibition of caspases by inhibitor of apoptosis protein (IAP) and is known to be a new and important regulator of...
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