ketones carboxylic acids and nitriles
Handbook of Polymer Synthesis Second Edition Episode 11 pptx
... corresponding vinyl monomer with cinnamoyl and crown ether groups [103] Copyright 2005 by Marcel Dekker All Rights Reserved 2 Ketones, Carboxylic Acids and Nitriles Metal acetylacetonates are covalently ... double-stranded DNA (details see [78, p 642]) Ru(phen)2þ binds strongly to double-stranded DNA, and minimal binding is observed in the presence of single-stranded DNA If a given single-stranded DNA ... complexes between TiCl4 and the copolymers of styrene and the diallyl esters of dicarboxylic acids [85] For n ¼ or 2, a mixture of complexes with the cis-disposition of and trans-disposition of...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
... fragment and fragment in the 4-kDa peptide tertiary structure, and comparison of the tertiary structure of insulin and the 4-kDa peptide (A and B) Fragment and fragment are shown in orange and red, ... fragment and fragment 2, which indicates that Val12 and Arg18 not belong to fragment and fragment and indicates that Val12 and Arg18 might play a different role from those residues of fragment and ... similar topology of side-chains of putative active residues in both insulin and the 4-kDa peptide (E and F) and b-strand, and fragment contains hairpin-b [14] These structures form the sheet of the...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
... 3, and 6) or left untreated (lanes and 4) Crosslinked cells were lysed and CD38 was immunoprecipitated from cells expressing CD38 (CD38-WT, lanes 1, 3, and 6) or lacking CD38 (CD38-KO, lanes and ... Hernandez-Hernandez for technical advice and Q F B ´ Hector Romero Ramı´ rez for technical assistance M E M.-G., A S.-T ´ and L S.-A are supported by CONACyT Mexico grants, # 28093N, 33497N and ... stained to determine CD38 expression levels and at least five individual clones were picked to expand and analyze experimentally Measurement of cyclase and glycohydrolase activity in Ba/F3 transfectant...
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báo cáo hóa học:" Unsaturated phosphatidylcholines lining on the surface of cartilage and its possible physiological roles" potx
... compare the three pairs, PLPC and DPPC, POPC and DPPC, and PLPC and POPC, separately There were significant differences between POPC and DPPC (p = 0.002), and PLPC and POPC (p = 0.003) There was ... that the total percentage of all saturated fatty acids was about 39% and the majority of fatty acids were unsaturated fatty acids (61%) As two fatty acids are needed to form an intact SPC or USPC ... The standard curves for all five PC species were all linear over the ranges of 5–25 mg/L and their correlation coefficients (r) were > 0.98 No internal standard was used The interassay and intra-assay...
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Báo cáo toán học: " Spin-related tunneling through a nanostructured electric-magnetic barrier on the surface of a topological insulator" potx
... magnetic barrier with B = 1, and V = Figure 4: The same as Figure 2, but now for a combined electric and magnetic barrier with B = 1, and V = Figure 5: Conductance and Fano factor of the single ... shown in Figure We can create magnetic and electric potentials underneath a superconducting plate and a ferromagnetic strip [17, 18] The relevant magnetic and electric fields are directed perpendicular ... V (x) = V [Θ(x) − Θ(x − d)]/2 and Bz (x) = B[Θ(x) − Θ(x − d)]/2, where Θ is the Heaviside step function The magnetic field is perpendicular to the surface and the Landau gauge A = (0, Bx, 0) is...
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Báo cáo lâm nghiệp: "Interactions of ozone and pathogens on the surface structure of Norway spruce needle" potx
... natural and that caused by air pollution, probably increases cuticular transpiration (Cape and Fowler, 1981) and accelerates winter desiccation and needle shedding (Lewitt, 1980; Huttunen and Laine, ... relationships between many plant and fungus species (Weidensaul and Darling, 1979; Dohmen, 1986) The interaction mechanisms between ozone and plant diseases are complicated and not very well understood, ... needles (Fig C and ozone D) Discussion Air pollutants, especially S-compounds are known to alter the structure of epicuticular waxes of conifers (Cape and Fowler, 1981; Huttunen and Laine, 1981;...
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Báo cáo y học: "Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture" doc
... infection and five as a result of aseptic loosening of the prosthesis On removal, the femoral and acetabular cup components of the prosthetic hip joint were placed into sterile plastic bags and immediately ... and School for analysis Several preoperative and perioperative samples were also taken from each patient, including hip joint aspirate, capsular fluid, acetabular membrane, femoral membrane and ... agar and CY-agar plates Columbia blood agar plates were incubated in 5% CO2 at 37°C, and FAA plates were incubated at 37°C in an anaerobic chamber with an atmosphere of 85% N2, 10% CO2 and 5%...
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Báo cáo y học: " HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses" pot
... Monoclonal antibodies 4E10 and 447-52D and soluble CD4 (sCD4) [19] Two human MAbs (4E10 and 447-52D) and sCD4 were kindly gifted from the National Institute for Biological Standards and Control (NIBSC, ... Institute for Biological Standards and Control (NIBSC), and National HIV Repository and Bioinformatic Center (Thailand) for providing monoclonal antibodies 447-52D, 4E10, sCD4, and HIV-1 isolates, respectively ... (AY243202) and MENO43 (AY243213) HIV-1 TCLA strains including, subtype A (92RW009 and VI191), subtype B (QH0692, SF162, IIIB and MN), subtype C (92BR025 and DU174), subtype D (92UG024) and CRF01_AE...
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Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
... days 3, 7, and 14 for PCR studies and days 0, 1, and for measurement of soluble HLA-DR Blood (7.5 ml) was collected in preservative free heparin (final concentration of 100 iu/ml) and cells were ... Mortality and focus of sepsis are described in the previous study [22] Sample preparation and flow cytometry Arterial blood was collected from septic patients on the day sepsis was diagnosed (day 0) and ... associated with impaired monocyte function and restoring expression to normal levels has been proposed to be beneficial [3] A candidate factor for activating monocytes and restoring functional indicators...
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Effect of urban emissions on the horizontal distribution of metal concentration in sediments in the vicinity of Asian large cities
... first, second and fourth locations counting from the mouth of the river consisted mostly of silt and clay with less than 20% sand content However, the third sample contained 64% of sand and other ... mean volume of around 10 micrometer and with lower than 17% sand content Figure 1(c), 1(d), 1(e) and 1(f) show the horizontal distribution of copper, nickel, lead and zinc concentrations in the sediments ... lead and zinc Table summarizes the results of all the target metals in this study Concentration of metals like copper and nickel other than lead and zinc were distributed in narrow ranges and...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
... Chaves S & Costa J (1991) Dissociation constants of Bronsted acids in D2O and H2O: studies on polyaza and polyoxa–polyaza macrocycles and a general correlation Anal Chim Acta 245, 271–282 Pierattelli ... group and the data obtained for redox titrations followed by visible spectroscopy at pH 7.0 and 8.1, were used to monitor the thermodynamic properties of DvHc3:Pi The fittings of both NMR and visible ... methyl groups 21CH3I , 181CH3II , 121CH3III and 181CH3IV both Desulfovibrio vulgaris cytochrome c3 (DvHc3) and DvHc3:Pi are reported in Fig by dashed and solid lines, respectively Figure shows...
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... antibody and particularly to appreciate the size of the cavity left around the iron atom and which kind of ligands it can accommodate; second, to measure the in¯uence of an axial ligand of the ... AI and A0, AI, and A are the absorbances of the initial, ®nal and mixed species, respectively The linearity of the graph representing 1/DA as a function of 1/[L]n was then assayed with n and ... added and the reaction was started by the addition of H2O2 at concentrations ranging between and 10 mM The initial rates of oxidation were then measured as above mentioned and the kcat and Km...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... CDRs of heavy chain and CDR3 of light chain [ref 39] Fv Randomization in CDR loops of heavy and light chains Y/S library in BC, DE and FG loops 10 FN3 Randomization in BC, DE and FG loops Fig Local ... (XGGGS)n in which the X residues were randomized and the linker length (n) was intermittently varied (B) DARPin: six amino acids in the loop and helix structures are randomized (C) A-domains: variable ... structures visualized by X-ray and NMR analyses We describe local library approaches as go-between techniques for grafted foreign peptide sequences and small scaffold proteins, and as methods for designing...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... substrate The ligands for Mn3 in MtSTP were Asp118, Asp191 and Ser160, the last coming from the flap domain [9] There are homologous residues to Asp118 and Asp191 in HsSTP (Asp146 and Asp239) and, of course, ... (St Louis, MO ⁄ YA-Kemia OY, Helsinki, Finland); Na2HPO4, KH2PO4 and CaCl2, JT Baker (Phillipsburg, NJ); NH4Cl, NaCl and MgSO4, Sigma-Aldrich; and amino acids, Merck ⁄ Calbiochem (Darmstad, Germany) ... modeled into SaSTP by aligning the HsSTP and SaSTP monomers active sites In the alignment metal ions M1 and M2, Wn, and carboxylate groups of Asp36, Asp192 and Asp231 were used Alignment of 12 atoms...
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Báo cáo khoa học: A kinetic approach to the dependence of dissimilatory metal reduction by Shewanella oneidensis MR-1 on the outer membrane cytochromes c OmcA and OmcB potx
... and omcB genes were 5¢-phosphorylated and cloned into EcoRV-digested and calf intestinal phosphatase-treated pKNOCK-Km and pKNOCK-Cm, respectively, using T4 DNA Ligase (all enzymes: New England ... Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen) J Van Beeumen and B Devreese are indebted to the Fund for Scientific Research (FWOVlaanderen) for granting research project ... 2), omcB– (lane 3), and omcA– omcB– (lane 4), and complemented strains omcA– ⁄ pBAD202 ⁄ D-TOPOomcA (lane 5) and omcB– ⁄ pBAD202 ⁄ D-TOPOomcB (lane 6) A molecular mass standard is indicated at...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... (Glu; strain HUT7212) and Glm (strain IFO 0111) from the GLU [13] and GLM [14] genes, consist of 492 and 489 amino acid residues, respectively, with a sequence identity of 60% and a similarity of ... essential for raw starch binding, the above amino acids were mutated and the mutants tested for their ability to adsorb to and digest raw starch Results and discussion Description of the structures ... for Glu1.1 and Glu-A estimated from the rA plot [18], estimated standard uncertainty (ESU) based on R and Rfree factors (the Cruickshank’s dispersion precision indicator DPI [19], and the average...
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Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
... Biochem 269) MATERIALS AND METHODS Materials Solvents and chemicals were purchased from Fluka, Buchs, Switzerland 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and spinlabelled phosphatidylcholines ... 15 min, C) and carefully resuspended in a buffer containing 10 mM Tris/HCl, pH 8.0, mM MgCl2 and 10% glycerol Insoluble material was removed by centrifugation (39 000 g, 15 min, C) and the enzyme ... of unlabelled POPC was adjusted and the different lipids mixed prior to evacuation Reconstitution of PCD-labelled F1F0 ATP synthase and PCD-labelled c11 in POPC and SLPC-containing vesicles The...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 and -3 were analyzed Cells over-expressing HSP70 and its deletion ... caspase-3 and stress-activated protein kinase ⁄ Jun kinase (JNK) activation [18] and mitochondrial depolarization [19], blocks apoptosome formation and activation of caspase-9 [20], and inhibits ... signals were quantified and normalized to GAPDH signals and are presented as the fold increase over the respective controls HS, Heat stress (B) Vector control (pcDNA3.1) and HSP70-over-expressing...
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