isolation reverse transcription and real time pcr analysis
Báo cáo y học: "Sequential emergence and clinical implications of viral mutants with K70E and K65R mutation in reverse transcriptase during prolonged tenofovir monotherapy in rhesus macaques with chronic RT-SHIV infection" pptx
Ngày tải lên :
13/08/2014, 09:21
... processing, data analysis and preparation of the first draft of the manuscript JAJ, JL and WH contributed all real- time PCR data including data analysis and interpretation EJB, RPS and TBM performed ... subpopulation Real- time polymerase chain reaction (PCR) for sensitive detection of K65R and K70E in plasma RT-SHIV RNA Sensitive testing for the K65R and K70E mutations was performed using real- time PCR- based ... AAA CAA TGG CCA TTG ACA G) for PCR amplification The real- time PCR reactions were performed in duplicate using l of the RT -PCR products for both the total virus copy and mutation-specific tests...
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Báo cáo khoa học: "METONYMY: REASSESSMENT, SURVEY OF ACCEPTABILITY, AND ITS TREATMENT IN A MACHINE TRANSLATION SYSTEM S" ppt
Ngày tải lên :
23/03/2014, 20:20
... systems such as the ULTRA MT system (Wilks and Farwell 1990) and the Metallel metonymic analysis program (Iverson and Helmreich 1992) Iverson, Eric and Helmreich, Stephen (1992) Metallel: An ... guideline for the analysis component, 2) language dependency, which can be used for generation, and 3) others such as the context, culture, and familiarity We think that it seems unrealistic to expect ... "Steinbeck" and "Bach" also belong to this group (see Appendix A 1.2) These sentences are fully acceptable in English and Japanese, however, their acceptability is low in Chinese and sentence...
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Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx
Ngày tải lên :
18/06/2014, 22:20
... glutamine and 1× antibiotics For T cell and monocytes separation, PBMCs were cultured in the same RPMI medium described above and then stained with anti-CD3 and anti-CD4 antibodies for T cell and anti-CD14 ... analyzed by TaqMan genotyping assay using the Real- time PCR system 7500 (Applied Biosystems, Foster City, CA, USA) The primers and probes for C2/CFB rs9332739 and C3 rs2230199 were from the inventory ... from AMD patients and controls were treated with or without C5a and a C5aR antagonist for days Cell supernatants from 14 controls and 14 AMD patients were used for ELISA analysis and are presented...
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báo cáo hóa học: " Formation of multinucleated giant cells and microglial degeneration in rats expressing a mutant Cu/Zn superoxide dismutase gene" ppt
Ngày tải lên :
19/06/2014, 22:20
... degeneration [8,9], and work by Cleveland and co-workers has generated findings, which show that toxicity to motor neurons requires damage from mutant SOD1 acting within nonneuronal cells [10] and, more ... PBS The spinal cord and brain were dissected out and fixed overnight in 4% paraformaldehyde at 4°C, transferred to 30% sucrose and then frozen Lumbar spinal cord, cortical, and brainstem sections ... (A-F) and in motor cortex with OX-42 (G) and OX-6 (H) during Visualization disease Visualization of microglia in midbrain with GSA-I-B4 lectin (A-F) and in motor cortex with OX-42 (G) and OX-6...
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Báo cáo y học: "The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus" potx
Ngày tải lên :
09/08/2014, 01:21
... germinal centre (GC) A and GC B, and B-cell clusters C and D were derived Groups of sequences were deemed to be clonally related when they used the same V, D, J and CDR3, and differed only by base ... spleen by staining with anti-CD20 and anti-FDC, respectively Two GCs (GC A and GC B) were excised and the rearranged VH genes amplified (as described under Materials and methods) Both GCs were located ... be seen in Fig 1, and has two distinct areas of staining for FDC and one large area of B cells encompassing both FDC regions Very few T cells could be seen within GC A and B, and no plasma cells...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
Ngày tải lên :
10/08/2014, 10:21
... material was included for RT -PCR, a final concentration of 0.5 μM of each forward and reverse primer and 0.2 μM of the TaqMan probe was used Cycling conditions involve reverse transcription at 50°C ... examination of liver tissues Gene expressions by qualitative and quantitative real time PCR for the following genes: b-catenin, PCNA, cyclin D and survivin genes Alpha fetoprotein by ELISA (provided ... antisense primer and 0.8 μl superscript AMV reverse transcriptase for 60 at 37°C Quantitation of gene expression was conducted using universal probe library sets based real time PCR (Roche diagnostics)...
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Báo cáo y học: "The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles" pot
Ngày tải lên :
11/08/2014, 08:21
... male and females so the results were pooled Mice were given food and water ad libitum, and housed with a 12 h day/night cycle The Animal Ethics Guidelines outlined by St Vincent's Hospital and ... was monitored and maintained at 36 ± 1°C After the ischemia period, the bands were removed and the mice allowed to recover After 24 h reperfusion the mice were re-anaesthetized and the gastrocnemius, ... gastrocnemius, soleus and EDL muscles were carefully removed from both treated and contralateral sides and weighed, before the mouse was sacrificed Age matched (12–24 wk, 25–35 g) male and female mice...
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Báo cáo y học: " Kinetics of hepatitis C virus RNA load during pegylated interferon alpha-2a and ribavirin treatment in naïve genotype 1 patients" potx
Ngày tải lên :
13/08/2014, 13:20
... to week 2, 4, 8, and 12 of treatment, week point time tend to be better than other time points for predicting SVR, with a PPV of 91% and a NPV of 89% and a very good sensitivity and specificity ... 1A and genotype 1B patients, and, in cases, genotype subtype was undetermined Statistical analysis The ROC curve method was used to determine the best cut-off between week 2, week 4, week 8, and ... have read and approved the content of this work Measurement of serum HCV RNA HCV RNA levels were measured at the same time on blood samples collected at baseline and at weeks 2, 4, 8, and 12 after...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
Ngày tải lên :
19/02/2014, 18:20
... 818–841) and 12-SEA-R 5¢-CCGCT CGAGTAGCACAGTATGGCCTTTC-3¢ (AF147790 1153– 1171) SpeI and XhoI sites were incorporated at the 5¢ end of the forward and reverse primers, respectively, and MUC3 and MUC12-specific ... MUC3- and MUC12- cleavage sites and the MUC3 and MUC12 SEA domains All constructs were based on the MUC1FDTR backbone [18,13], and are illustrated in Fig Using unique PsiI (J05582: 3351) and DraIII ... consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage site, which is located in a loop between b2 and b3 strands, are illustrated...
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Child-friendly health care: the views and experiences of children and young people in Council of Europe member States doc
Ngày tải lên :
17/03/2014, 17:20
... Bosnia and Herzegovina, Bulgaria, Estonia, Finland, France, Georgia, Germany, Greece, Ireland, Italy, Malta, Netherlands, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain and the ... logistical and methodological – and it is important that the Council of Europe and other organisations continue to learn from, and improve, their work in this field How the bodies who ask children and ... care and fulfil commitments under the United Nations Convention on the Rights of the Child (UNCRC) and other international standards At its meeting in December 2010, the Committee of Experts mandated...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
Ngày tải lên :
18/03/2014, 01:20
... TGCTGGAGTCTCGCCTTCGCAAC-3¢; and KE2RS2, 5¢-GCATGCTGGAGTCTCGCCTTCGCAACAGGGCC ACCAGTA-3¢ PCR was preformed with mIID-5¢ and KE2 or KE2RS2 using mouse sPLA2-IID cDNA as a template Each PCR product was ligated into pCR3 .1 ... 5¢-GGCCTAGGAGCAGAGGATGTTG-3¢; and human sPLA2-X sense, 5¢-ATGCTGCTCCTGCTAC TGCCG-3¢ and antisense, 5¢-TCAGTCACACTTGGGC GAGTC-3¢ PCR conditions were 94 °C for 30 s and then 30 cycles of amplification at 94 °C for s and ... sPLA2-IID (mIID) and its mutants, KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2, respectively (B) Expression of the wild-type (WT) and two mutants...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
Ngày tải lên :
23/03/2014, 13:20
... the transcriptional activity of each transcription factor alone Experimental procedures Plasmid constructs and sequence analysis Effector constructs for transient transfections of mammalian and ... coactivators Thus all the transcription factors known to bind the IFN-b gene promoter in virus-infected cells can be expressed and activate transcription in insect cells, and the p300 and CBP coactivators ... 6%) and –C2 ( 6%) (but weak binding to CBP-C, 1%), and to p300–N ( 11%), –C ( 14%), –C1 ( 7%) and –C2 ( 27%) Synergy between ATF-2/c-Jun and IRF-3/IRF-7 The affinity of ATF-2/c-Jun and...
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Báo cáo khoa học: Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover pptx
Ngày tải lên :
23/03/2014, 18:20
... DNA and cloned into the high-copy number plasmid YEplac181 [19] Using primers F(SFH1-EGFP) and R(SFH1-EGFP) (Table 4) a 2663 bp SFH1-EGFP PCR fragment was generated, cut with PstI and BglI and ... localized by subcellular fractionation using standard techniques of organelle isolation [24] and Western blot analysis (Fig 5) EGFP hybrids of SEC14, SFH2 and SFH5, which were localized mainly to the ... the SEC14 gene and 879 bp downstream of SEC14 The SFH1 ORF region was generated by PCR using FY1679 chromosomal DNA as a template PCR primers were 5¢-GGCAT GTGGGTGAATTACAA-3¢ and 5¢-TTTGGGCCGGTT...
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Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) potx
Ngày tải lên :
02/04/2014, 22:20
... J and et al (2000).; Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germs layers Mol Med 6, 88 - 95 Itkin - ansari, P., et al (2001); PDX - and ... la Tour, D., et al (2001); B - cell differentiation from a human pancreatic cell line in vitro and in vivo Mol Endocrinol 15, 476 - 483 Dzierzak, E et al (1998); Embryonic beginnings of definitive ... khảo Amit et al (2000); Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture Dev Biol 227, 271 - 278 Beltrami, A...
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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt
Ngày tải lên :
18/06/2014, 15:20
... receptor ligands Sigma-2 specific ligands SV119, SV95, and fluorescent labeled sigma-2 ligand, SW120, were synthesized and prepared as previously described [13-15] The Sigma-1 receptor ligand, (+)-pentazocine ... resistance to many standard cancer treatments [12,18-21] Standard therapies including most chemotherapeutics and radiation therapy induce cellular stress and thereby promote apoptosis Standard therapies ... receptors exist: sigma-1 and sigma-2 subtype [5] These subtypes display different tissue distributions and distinct physiological and pharmacological profiles in both the central and peripheral nervous...
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báo cáo hóa học: " Proinflammatory and proapoptotic markers in relation to mono and di-cations in plasma of autistic patients from Saudi Arabia" docx
Ngày tải lên :
19/06/2014, 22:20
... Mean with the standard error bars of measured Caspase3, IL6 and TNFa (a), Ca2+, Mg2+ and Ca2+/Mg2+(b), and Na+, K+ and Na+/K+(c) in autistic patients (N = 25) compared to age and gender matching ... Caspase3 and IL6 (positive correlation); (b): Caspase3 and TNFa (positive correlation); (c): Caspase3 and Ca2+ (positive correlation); (d): Caspase3 and K+ (negative correlation); (e): Caspase3 and ... mechanism relating Ab and Ca2+- induced apoptosis in brain cells of Saudi autistics Table ROC analysis of Ca2+/Mg2+ and Na+/K+ ratios and Caspase3, IL6, TNFa, Ca2+, Mg2+, Na +and K+ in autistic groups...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
Ngày tải lên :
20/06/2014, 04:20
... for min, and cooled at 5°C for Real- time Quantitative RT -PCR Reaction (qRTPCR) μl of cDNA from the RT reaction was added to 20 μl real- time quantitative polymerase chain reaction (qPCR) mixture ... Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis ... μl of 2× SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA) and 12 pmol oligonucleotide primers PCRs were carried out in a Bio-Rad MyiQ Single-Color Real- Time PCR Detection System...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
Ngày tải lên :
20/06/2014, 07:20
... for min, and cooled at 5°C for Real- time Quantitative RT -PCR Reaction (qRTPCR) μl of cDNA from the RT reaction was added to 20 μl real- time quantitative polymerase chain reaction (qPCR) mixture ... Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis ... μl of 2× SYBR® Green PCR Master Mix (Applied Biosystems, Foster City, CA) and 12 pmol oligonucleotide primers PCRs were carried out in a Bio-Rad MyiQ Single-Color Real- Time PCR Detection System...
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