intrinsic stability structures of the major degradation products
Báo cáo khoa học: Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion doc
Ngày tải lên :
30/03/2014, 15:20
... of the digestion products, HPLC analysis showed the characteristic large (peak 7) and small (peak 5) subunits of the native 2S albumin at the start of digestion (Fig 3C) After 120 min, some of ... using synthetic peptides, determined the location of four immunodominant CD4+ T-cell epitopes in the unprocessed precursor of the Brazil nut 2S albumin One of these epitopes matched with the signal ... as the starting material Although it has been described that the pH of the duodenum may vary within the range 5–7 [27–29], the most accurate range seems to be 6–6.5 [30–34] Therefore, the pH of...
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THE STRUCTURES OF THE SENTENCES IN ENGLISH 9.doc
Ngày tải lên :
17/08/2013, 23:10
... questions (if/ whether ) Notes: S + asked ( O) Wanted to know + If/ whether + Wondered S+V Change in adverbs and articles this these here now tomorrow today → that → those → there → then → the following ... store the following day Question: - “what is your name?” the teacher asked me = >The teacher asked (me)what my name was Note: She said, “Are you thirsty, Mary?” She asked Mary if (whether) she ... Compare: (hai vật ngày phát triển song song) Form: Short adj: The adj +er + S+ V, The adj +er + S+ V Long adj: The more +adj + S+ V, The more +adj + S+ V 32 V + preposition be amazed at / be amused...
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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
Ngày tải lên :
18/02/2014, 08:20
... of the structure are not seen on the map, probably owing to disorder of these regions The overall geometry of the model according to the procheck program [21] is of good quality, with 95.4% of ... to elucidate the mechanism of the elevated thermostability of TtAKb In this article, we describe the crystal structures of TtAKb in two forms, Thr-bound and Thr-free, and discuss the regulatory ... regulatory mechanism of Thr and the structural features responsible for the high thermostability of TtAKb Results and Discussion Model quality The crystal structure of the Thr-bound form of TtAKb ˚ (TtAKb-Thr)...
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
Ngày tải lên :
21/02/2014, 01:21
... for the understanding of the genetics of this significant food-borne pathogen We therefore elucidated the structures of these major cell surface carbohydrates to make functional analysis of the ... placed the sole a-D-Gal sidebranch at O-2 of the unit with the anomeric at d 4.99, which has to belong to the sole 2-substituted unit, that being the 2,3-disubstituted Gal in the backbone The other ... observation in the linkage analysis that the 6-O-Me-Hep was a terminal unit The fragment ion at 382 arose from the loss of both the 6-O-Me-Hep and GalNAc, which then lead to the loss of Rib with...
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Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
Ngày tải lên :
06/03/2014, 00:21
... form of the enzyme runs along the axis of the b-propeller through the whole domain and is only blocked by Cys236 in the area of the subsite )1 binding the fructose molecule A number of structures ... inside of it, which resembles the shape of a fishtrap The active site is located at the bottom of the axial funnel created by the interblade loops and the loops between b-strand 2–3 of each blade The ... results of MS of recombinant b-fructofuranosidase are close to the theoretical value of the molecular mass A peak of molecular ion at m ⁄ z = 58 879 Da was observed on the MALDITOF mass spectrum The...
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Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc
Ngày tải lên :
07/03/2014, 09:20
... j is defined by the number of nucleotides between the end of the i-th gene and the start of the j-th gene along the chromosomes We then derived a distance kernel using the formula Kposition (xi,xj) ... application of this technique, we attempted to reconstruct the enzyme gene network of the lysine -degradation pathway in P aeruginosa We filled in some of the enzyme genes in the lysinedegradation ... evaluated the accuracy of the prediction using ROC scores, defined as the area under the receiver operating curve (ROC), that is, the area under the plot of true positives as a function of false...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the ... conclude that the inflammatory state of the lung influence on the rate of metabolism and clearance of Der p Thus, an allergic response may lead to prolonged retention of the allergen in the airways ... airways of Der p 2-sensitized and nonsensitized mice The fate of the allergen could be followed in vivo both at the whole-body level and at the molecular level, through the application of a newly...
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Báo cáo khoa học: Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin⁄26S proteasome-mediated proteolysis potx
Ngày tải lên :
15/03/2014, 09:20
... regarding the biochemical properties of the major ubiquitin receptors from different species, in terms of their selectivity for linkage types and the lengths of the ubiquitin chains and their associated ... RPN13 The potential involvement of the hydrophobic patch in the UBL of hHR23b was also examined The UBL variants constructed for analysis of the association of RPN10 with human hHR23b and the DSK2 ... which each of the UBAs of Arabdopsis RAD23 contributes additively to the binding of K48-linked ubiquitin chains (Table and data not shown) The roles of the UBAs in ubiquitin chain binding of human...
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Báo cáo khoa học: Zinc-binding property of the major yolk protein in the sea urchin ) implications of its role as a zinc transporter for gametogenesis ppt
Ngày tải lên :
16/03/2014, 05:20
... zinc Indeed, 33% of the total zinc in the ovary at stage and 13% of the total zinc in the testis at stage is still bound to MYP On the basis of the results of the present study and others, we propose ... measured the protein content of the CFMYP and EGMYP bands by densitometry of the SDS ⁄ PAGE gel of the purified MYP, and the ratio of CFMYP to total MYP (CFMYP + EGMYP) was calculated (Fig 3B) In the ... 1.7% of the MYP was CFMYP at stage and 18.3 ± 2.6% at stage In the testes, the proportions of CFMYP were 17.7 ± 5.3% at stage and 6.9 ± 3.5% at stage The higher the proportion of CFMYP, the more...
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Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx
Ngày tải lên :
16/03/2014, 13:20
... op Zoom, the Netherlands) Ca2+ binding of the enzyme was studied at Yobs ẳ aU ỵ bU Tị ỵ Thermal stability Thermal stability was studied by incubating SPC4 in the absence or presence of mm CaCl2 ... is needed to establish the proximal calcium binding site The binding of Ca2+ has been proposed to change the electronic properties of the heme iron or the topology of the heme vicinity and might ... increases the activation energy of heat inactivation of SPC4 from 157 12170 14 kJặmol)1 The increased stability of SPC4 in the presence of Ca2+ ions was conrmed by uorescence experiments Upon Fig Thermoinactivation...
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Báo cáo khoa học: Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution ppt
Ngày tải lên :
16/03/2014, 18:20
... with the Gly93 of another The crystal structures of diubiquitin and tetraubiquitin showed some alternatives of the quaternary conformations of ubiquitin polymer for efficient recognition by the ... is particularly evident in the regions of 28–43 and 71–83, that correspond to the strand b2, the N terminus of the helix a1, the helix a2, and the connecting loop to the strand b5 (Fig 3A,D) Such ... structure of human SUMO-2 is more similar to that of yeast SMT3 than to the NMR structure of SUMO-1 The difference between SUMO-2 Table Refinement procedures of the SUMO-2 crystals Description of steps...
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Báo cáo khoa học: Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1 pdf
Ngày tải lên :
16/03/2014, 18:20
... for the development of allergy vaccines which are based on the reduction of allergen fold Results Comparison of natural and recombinant group grass pollen allergens Although the majority of rPhl ... for the design of safe allergy vaccines with reduced allergenic activity Disruption of the native structure of respiratory allergens allows for the maintenance of important T cell epitopes of ... preincubation of patients’ sera with small recombinant protein fragments suggesting the importance of conformational IgE epitopes [25] Therefore, we further tested the importance of structural...
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Báo cáo khoa học: Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups ppt
Ngày tải lên :
16/03/2014, 23:20
... of GalA GLC analyses of the acetylated (S)-2-(+)-butyl glycosides demonstrated the d configuration of Glc, GlcN, GalN, and GalA The d configuration of Qui3N was established by the analysis of the ... Proteus [16] Studies of the O-polysaccharides of Proteus genomospecies and and the comparison of the corresponding 13C NMR spectra indicated that they are identical to those of P vulgaris O8 [17] ... Proteus genomospecies Fig Part of a 2D ROESY spectrum of the polysaccharide of Proteus genomospecies The corresponding parts of the 1H-NMR spectrum are displayed along the axes Arabic numerals refer...
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Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf
Ngày tải lên :
18/03/2014, 01:20
... splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence Cell 27, 487–496 27 Michel, F & Westhof, E (1990) Modelling of ... splicing pathway We predicted these RNA species to represent the 5¢ half and the 3¢ half of the excised intron, an assumption based on the estimated size and on the fact that the similarly organized ... slightly slower than the 1.2-kb precursor RNA (Fig 3A), but consists only of the 389-nucleotide IC1 ribozyme lacking the first 551 nucleotides of the intron (four of four clones; Fig 4B) The RNA 1b circle...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
Ngày tải lên :
23/03/2014, 15:20
... majority of the sulfated sugar In conclusion, the results obtained provide evidence of the nature of the glycans present in the unique N-glycosylation site present in the C-terminal domain of cruzipain ... [14] The fact that cruzipain is a complex mixture of isoforms with a great diversity in the N-linked structures made the separation of species very difficult We took advantage of UV-MALDI-TOF mass ... confirm the nature of the substitution present in glycans obtained from cruzipain, desulfation was carried out using sulfatase The UV-MALDI-TOF spectra of the treated sample, obtained in the negative...
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Báo cáo khoa học: High-resolution crystal structures of the flavoprotein NrdI in oxidized and reduced states – an unusual flavodoxin pot
Ngày tải lên :
29/03/2014, 21:20
... other NrdI sequences Thus, the extended loop may close off the flavin binding site by folding back over the core of the protein When the present paper was in revision, the crystal structures of ... electron density for the FMN cofactor is excellent in both structures, and the high resolution of the oxidized form allows confirmation of the protonation Results The crystal structure of baNrdI has ... without inclusion of explicit hydrogen atoms on the cofactor, the difference electron density can clearly be seen at 2.5 r for many of the aliphatic hydrogen atoms of the cofactor (Fig 2A) By...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... counting the number of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation ... stimulation of 200 pulses at Hz and 0.8 mA The measured latency was the time span from the stimulation to the beginning of the first wave, whereas the amplitude was the voltage difference between the ... differentiation of forelimb foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit CSF aspiration 80 μL of...
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THE STRUCTURES OF THE SENTENCES IN ENGLISH ppsx
Ngày tải lên :
05/07/2014, 05:20
... have to Change in adverbs and articles this → that these → those here → there now → then tomorrow → the following day today → that day yesterday → the day before… past simple Present Progressive ... store the following day Question: - “what is your name?” the teacher asked me = >The teacher asked (me)what my name was Note: She said, “Are you thirsty, Mary?” She asked Mary if (whether) she ... Compare: (hai vật ngày phát triển song song) Form: Short adj: The adj +er + S+ V, The adj +er + S+ V Long adj: The more +adj + S+ V, The more +adj + S+ V 32 V + preposition be amazed at / be amused...
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Báo cáo y học: "Critical role of the major histocompatibility complex and IL-10 in matrilin-1-induced relapsing polychondritis in mice" doc
Ngày tải lên :
09/08/2014, 01:24
... investigate the influence of gender, female mice on the B10.Q background were immunized at the same time as their male littermates These females developed disease symptoms less severe than those of the ... mice Approval for the animal experiments was obtained from the ethical committee at Lund University Induction of disease Mice were immunized at the base of the tail with 100 µg of matrilin-1, purified ... in the acute phase at score or at the end of the experiment at day 130 The tissue was immediately either snap-frozen at -70°C or fixed in 4% paraformaldehyde solution for 24 hours and further...
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Báo cáo y học: "Disruption and pseudoautosomal localization of the major" ppt
Ngày tải lên :
14/08/2014, 08:20
... are the swine leukocyte antigen (SLA) complex, where the centromere of chromosome separates the class II region from the other parts of the MHC [34] and the recently described class I genes of the ... two of these small PARs in platypus BAC2 was mapped to the PARs of Y4 and the short arm of X5 This fits with the orientation of X5 in the chain where the short arm lies towards Y4 [21] Together ... genes They found eight 200-900 kbp scaffolds that contained orthologs to many of the genes in the eutherian MHC The interpretation is that the syntenies of the MHC region are ancient and predate the...
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