... whereas fibronectin and a 30-kDa fibronectin fragment having heparin-binding ability showed interactionwith tendon decorin (Fig 4) Fibronectin is known to interact with decorin protein core [45] ... relevance of Lys/Hyl residues both in collagens and peptides for interactionwith decorin is in contrast with the findings that the binding site for decorin is located in a sequence within the peptide ... helical domain) On the contrary, CB7 and CB6 lie in the C-terminal half of the chain Binding specificity is demonstrated by the following (a) The absence ofinteractionwith decorin(s) of some peptides...
... differences were observed in the detected proteins Determination of partial amino acid sequences of binding proteins of dicalcin Purified binding proteins of dicalcin were digested with lysyl endopeptidase ... the interactionof frog annexin A1 and annexin A2 with dicalcin Consistently, we observed that the 35 kDa forms of annexin A1 and annexin A2 found in the fraction of the binding proteins of dicalcin ... proteins Fig Ca2+-dependent binding of recombinant annexins to dicalcin The cell lysate of E coli (lysate) expressing recombinant annexin A1, annexin A2 or annexin A5 was mixed with dicalcin-Sepharose...
... microscopy [15] In this study, we measured the interactionof stefin B with various combinations of phospholipid monolayers and bilayers Interactionof stefin B in the prefibrillar aggregated state with model ... membranes Fig Insertion of stefins into DOPC and DOPG monolayers (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of mNÆm)1 The proteins were injected into the subphase ... FEBS Fig Insertion of stefin B in prefibrillar form into an air–water interfaceInsertion into the air–water interface was measured in 10 mM Hepes, 200 mM NaCl, pH 7.5 with constant stirring at room...
... characteristic of the TÆT minorgroove than the AÆA minor-groove, in the binding of DAPI [16] In conclusion, the main cause of the strong inhibitory effect of DAPI on DNA replication in vitro reported in ... sequences within introns can regulate transcription [19,20], the prevalent location of ATT triplet repeats within introns in the human genome [18] suggests strongly that binding of DAPI to ATT-bearing ... melting studies The addition of ATT-template to DAPI solution determines drug fluorescence changes that are distinctive of the drug binding into the minor-groove of the double-helix structure of...
... immunoprecipitate, indicating that the interactions of these different proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP Association of 42Sp50 with ... [31–33] Together with the findings obtained in the present study, interactionof VICKZ and poly(A) binding proteins thus appears to define a conserved feature of mRNPs forming in different biological ... presence of 42Sp50 in nucleus and cytoplasm indicates that it might be a shuttling protein with a function in the nuclear export of localizing mRNAs To determine whether 42Sp50 is capable of shuttling,...
... chemoattractants in their fibroblasts J Immunol 2002,168:942-950 Kaufmann J, Franke S, Kientsch-Engel R, Oelzner P, Hein G, Stein G: Correlation of circulating interleukin 16 with proinflammatory cytokines in ... patients with rheumatoid arthritis Rheumatology 2001, 40:474-475 Lard LR, Roep BO, Toes RE, Huizinga TW: Enhanced concentrations of interleukin 16 are associated with joint destruction in patients with ... determines the disease specific immune response Given the variety of signaling pathways initiated by IGF-1 in fibroblasts, it may be speculated, as the authors mention briefly, that binding of antibodies...
... Annotation of grapevine Gene Index Annotation of grapevine Gene Index (VvGI version 5) using the MapMan ontology was performed by including information on grapevine genome and plant protein domains, ... relatively weak This is in line with the hypothesis that defence compounds are induced in resistant as well in susceptible interaction, the main difference being the speed and intensity of response Also, ... expression analyses in individual grape (Vitis vinifera L.) berries during ripening initiation reveal that pigmentation intensity is a valid indicator of developmental staging within the cluster Plant...
... Annals of Mathematics, 168 (2008), 367–433 The distribution of integers with a divisor in a given interval By Kevin Ford Abstract We determine the order of magnitude of H(x, y, z), the number of integers ... a satisfying (i), L (a; η) consists of a disjoint union of intervals, each with length ≥ η Breaking long intervals into shorter ones, we may 1 partition L (a; η) into intervals each with length ... For fixed σ, θ(σ, t) is decreasing, ν(σ, t) is increasing and G(θ)/θ is increasing, as functions of t Thus, we have the following 389 INTEGERS WITH A DIVISOR IN AN INTERVAL Lemma 3.9 Suppose y...
... protein (EGFP) reporter vector (pcDNA3 ⁄ EGFP-PPP6C-3¢UTR), in which the 3¢-UTR fragment of PPP6C, including the region encoding the putative binding site, was inserted downstream of the EGFP coding ... encoding the miR-373 binding sites (Fig 4A) Neither blocking of miR-373 with ASOs nor overexpression of miR-373 had any effect on the intensity of EGFP fluorescence from the vector containing mutations ... G1-phase and an increase in the proportion of cells in S-phase These findings indicate that PPP6C decreases the proliferation of HCC cells by inducing cell cycle arrest at the G1 ⁄ S checkpoint, which...
... expression in platelet membranes indicated that the mechanisms involved in ATP and ADP hydrolysis vary with the cholesterol level In fact, up to 250 mgÆdL)1, there was no increase in CD39 expression in ... High-density lipoprotein cholesterol was measured in the supernatant plasma after precipitation of apolipoprotein B-containing lipoproteins with dextran sulfate and magnesium chloride according to Bachorik ... analyzed with a FACScalibur flow cytometer using cellquest software (Becton Dickinson, San Jose, CA, USA), without fixation Hematologic determinations Quantitative determinations of platelets obtained...
... molecular basis of the carbohydrate cross-linking properties of the lectin In this study, the sugar-binding ability and specificity of each of the two sugar-binding sites in EW29Ch for certain sugars ... that the protein has two sugar-binding sites in subdomains a and c [24,25] Therefore, determination of the sugar-binding ability and specificity for each of the two sugar-binding sites in EW29Ch is ... binding site has been found in ricin; thus, the ricin B1 domain of the ricin B chain has two sugar-binding sites in the same molecule [22,23] Because sugars bound at the third sugar-binding site...
... ester-induced disruption of the CD4–Lck complex occurs within a detergent-resistant microdomain of the plasma membrane Involvement of the translocation of activated protein kinase C isoforms ... studies showing that EGFR is initially concentrated in caveolae within lipid rafts, but rapidly moves out of this membrane domain in response to EGF [7] The inefficient movement of ErbB2 out of these ... findings support the view that GM3 is mainly involved in retaining ErbB2 in lipidraft domains, but that it is not involved in ErbB2 phosphorylation EGFR and Shc-p66 preferential association with...
... consequences of HIP/PAP expression inhepatocellular carcinoma, we looked for proteins capable of interacting with this protein by screening a human HCC cDNA expression library in kgt11 using [32P]chimeric ... were therefore performed using this cell line HIP/ PAP was recovered in the serum of patients withhepatocellularcarcinoma which suggested that, in an in vivo setting, HIP/PAP was secreted by ... therefore looked for proteins capable of interacting with HIP/PAP inhepatocellularcarcinoma cells By screening a HCC cDNA library expressed in E coli with [32P]FlagHMK-HIP/PAP(29–175) as a...
... III by way of binding of JH III or closely related structures to the ligand binding pocket of USP [18] This binding of JH III by USP induces change in USP tertiary conformation [18] and induces ... receptors, including human RXR (which is the ortholog of USP) have shown that an area on the surface of the ligand binding domain involving the C-terminus of a-helix 3, a-helix 4, and the N-terminus of ... (L314) in putative coactivator-binding hydrophobic groove of USP (A) Activity of DR1JHECore promoter in Sf9 cells cotransfected with the indicated increasing concentrations of plasmid expressing...
... cycles were initiated by addition of and 10 nmol of INT Reoxidation of reduced heme b with 10 nmol INT was twice that with nmol INT, indicating proportionality over this range of INT concentration ... flavocytochrome b interact with O2 to generate oxidized INT (INTox) and O2– [15,17] according to reaction 1: INT• + O2 « INTox + O2– Eliminating O2– with SOD displaces the reaction to the right, with formation ... reaction 2: INT• + INT• + H+ fi INTred + INTox At low concentrations of INT, reaction predominates, whereas at high concentrations of INT reaction (which is second order with respect to the INT concentration)...
... were made with primary antibody replaced by PBS eral, the cases with intensive staining of CD133 had higher percentage of CD133+ tumor cells Several nests with intensive CD133 staining, so-called ... be seen within the field of cancer nests microscopically (Fig 1A to 1D) The cancer cells within an adenocarcinoma nest could actively proliferate and form a group of cells, which invaded into the ... the basal surface of cancer cells; (E): the staining of CD133 on budding cancer nest; (F): the staining of CD133 on poor-differentiated cancer nests with ductal structures Page of (page number...
... illustrating the fabrication of the microfluidic device containing PDMSinsulating microstructures Microfabrication and Experimental Setup The microfluidic device containing the insulating microelectrodes ... Instruments Inc., RI) Solutions of 1.0–10.0 mS/m were used in the experiments To begin, a suspension of fluorescent microspheres was injected into the inlet reservoir of the microfluidic chip with ... behavior within the microchannels of the insulating structure for increasing electric fields Dark areas are the solid insulators and bright areas are the microchannels filled with flowing fluorescent...
... learning will be demonstrated as the examples of the integrated system capability ROBUST MULTIPRIMITIVE FACE DETECTION AND TRACKING In intelligent systems, human-computer interaction including ... clips of almost exactly the same starting to ending frames many times to the face detection and tracking module as in Figure with different DFFS bound settings and with/ without Kalman tracking The ... and outdoor test sequences on single-frame and tracking-based settings DFFS bound Single-frame Indoor Tracking Single-frame Outdoor Tracking Single-frame Total Tracking Faces Detected F Pos Faces...
... Further inclusion criteria were normal levels of hemoglobin, leukocytes, platelets, creatinine, urea, GGT, AP, AST, ALT, bilirubine, creatinine clearance > 50 ml/min and no other clinically leading ... combination in prostate cancer using the highest Food and Drug Administrationapproved dose of 800 mg celecoxib daily Staging examinations The pre-therapeutic staging examinations included the initial ... were included into the study All 22 patients completed the radiotherapy without treatment break In all cases the intake of celecoxib started at the first day of radiotherapy in the morning Within...
... feelings of sadness or gloominess) on item (reported sadness) of the MADRS 28 Schz Initial PANSS Total score of ≥ 75, a minimum score of ≥ on one of the PANSS positive, and a minimum score of ... antipsychotics in maintaining treatment response Maintenance of response is an outcome measure that adds depth and dimension to our understanding of efficacy in the treatment of schizophrenia ... "successful treatment" was defined using CGI score-based thresholds [3] In this analysis, we assess cumulative time spent in response and time maintaining response, defining http://www.biomedcentral.com/1471-244X/9/13...