interaction of shock wave with 2 d wedge

... particle D2 was in all cases between 0.6 and 1.7 ms, similar to the expected diffusion time of lipid SUVs However, F2 and N2 differed markedly depending on the lipid used In the group of lipids with ... for 20 times using a mini-extruder syringe device (Avanti Polar Lipids) The extruded lipid solutions were diluted and mixed with 20 0 nM V4-TMR to study the interaction of peptide and lipid vesicles ... overlapped with that of labeled DPPG LUVs When the peptide/lipid ratio increased to 1 :20 , the amplitude of the ACF rose and the diffusion time increased compared with DPPG vesicles The amplitude of...

Ngày tải lên: 14/09/2015, 23:32

... h ade6-M216 leu 1- 32 ura4 -D1 8 h– ade6-M210 leu 1- 32 ura4 -D1 8 h+ ade6-M216 leu 1- 32 ura4 -D1 8 ntp1+:Ha6H (ura4+) h– ade6-M210 leu 1- 32 ura4 -D1 8 ntp1+:Ha6H (LEU2) h) ade6-M216 leu 1- 32 ura4 -D1 8 ... was cleaved with XhoI and NotI and cloned into plasmids pIH-ura and pIH-LEU These are integrative plasmids derived from plasmid pDS472a [20 ], without nmt promoter and ars1 sequences, and with ura4+ ... ade6-M216 leu 1- 32 ura4 -D1 8 tps1+:Ha6H (ura4+) h+ ade6-M216 leu 1- 32 ura4 -D1 8 ntp1+:Ha6H (LEU2) tps1+:GST (ura4+) h+ ade6-M216 leu 1- 32 ura4 -D1 8 tpp1+:Ha6H (ura4+) h– ade6-M210 leu 1- 32 ura4 -D1 8...

Ngày tải lên: 08/03/2014, 23:20

... drops of D2 O [20 ], and those broad signals were unchanged by the addition of a- and b-CDs FEBS Journal 27 2 (20 05) 6154–6160 ª 20 05 FEBS Y Izutani et al Interaction of gymnemic acid with cyclodextrins ... and studied CDs are a-, b- and c-CDs, consisting of 6-, 7- and 8-glucosyl units, respectively [14] CDs can be described as toroidal, hollow, truncated cones with a hydrophilic surface and a hydrophobic ... dependence of c-CD on NMR spectra of 2. 0 mM GA The ratios of c-CD to GA are indicated FEBS Journal 27 2 (20 05) 6154–6160 ª 20 05 FEBS In order to further analyze the aggregated property of GA and...

Ngày tải lên: 16/03/2014, 14:20

... amino acid sequence of the PDE5A1 DQGD(778–781) site and the corresponding region in PDE2A3, PDE4C, PDE 4D, PDE6ab and PDE11A1 D7 78 at P4 of the caspase-3 consensus site in PDE5A1 is replaced with ... residues in the PDE4B2 reference structure (H234, H238, H274, D2 75, E304, H307, T345 and D3 92) with little deviation The coordinates of these residues and the a-carbon centers of Q765, Q779 and ... transfected with pCAGGS-Casp-3 cDNA (1 lg) and/or wild-type pcDNA-3.1-PDE5 (5 lg) plasmid constructs Cells stimulated with and without staurosporine (10 lM, 24 h) were transfected only with wild-type...

Ngày tải lên: 17/03/2014, 09:20

... (nm) 2. 08 1.55 2. 54 3.35 3.43 3.44 2. 97 2. 53 1.95 2. 25 2. 29 2. 12 1.47 2. 55 3.78 5.61 5.05 4.46 3.57 2. 45 3.68 4.35 a From [54] bFrom [55] compounds studied and the tryptophan residue was obtained ... (solid line 0; dashed line, 1.66; dotted line, 4.98; dashed ⁄ dotted line, 8 .26 ; + + + +, 11. 52; short dashed ⁄ dotted line, 14.75) (B) Emission spectra of daidzein–HSA complex with increasing ... anisotropy of daidzein bound to HSA TIB decreased the anisotropy of daidzein from 0.16 to 0.08 The anisotropy of free daidzein was 0. 02 Hence, TIB partially displaced the daidzein in HSA (Table 2) The...

Ngày tải lên: 23/03/2014, 11:20

... facilitate the H2O2-induced oxidation of a Sp1 transcription factor fragment [22 ] While interactions of selenium compounds with redox-regulated transcription factors have been repeatedly discussed to be ... Roses, France and XPA was kindly ´ provided by Dr Andre Eker, Erasmus University of Rotterdam, the Netherlands This work was supported by grants Ha 23 72/ 1-3 and Ha 23 72/ 3 -2 from the Deutsche Forschungsgemeinschaft, ... min) with ethanol containing 125 mM sodium acetate, the DNA was centrifuged for at 7000 g The supernatant was discarded and the DNA pellet resuspended in enzyme buffer Damage induction and all...

Ngày tải lên: 30/03/2014, 15:20

... 20 100 70 : 20 : 10 20 .7 ND ND ND ND ND 14.0 12. 0 5.8 3.0 3.3 3.1 12. 6 12. 7 7.1 2. 9 1.9 2. 5 14.4 11.1 7 .2 4.0 1.8 2. 7 11.3 10.4 ND 11.5 11.1 ND 11.1 13.0 ND 2. 3 1.1 2. 2 11.5 12. 0 ND 2. 7 2. 1 2. 8 ... of charge and hydrophobicity in peptidelipid interaction: a comparative study based on tryptophan uorescence 29 26 B Christiaens et al (Eur J Biochem 26 9) 16 17 18 19 20 21 22 23 24 25 26 27 28 ... CDNN program [24 ] from [Q ]22 2 according to Chen et al [25 ] b a [Q ]22 2b 29 61 67 13 22 34 Helical content as calculated ể FEBS 20 02 Penetratin: interaction with model membranes (Eur J Biochem 26 9)...

Ngày tải lên: 31/03/2014, 23:20

... HANDBOOK OF STOCHASTIC ANALYSIS AND APPLICATIONS STATISTICS: Textbooks and Monographs D B Owen, Founding Editor, 19 72- 1991 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 ... 22 4 4.6 .2 SDEs with anticipating drift and initial condition 22 7 Bibliography 22 9 Numerical Analysis of SDEs Without Tears 23 7 H Schurz 5.1 Introduction 23 7 5 .2 ... Distribution: Second Edition, Revised and Expanded, Jagdish K Patel and Campbell B Read 151 Bayesian Biostatistics, edited by Donald A Berry and Dalene K Stangl 1 52 Response Surfaces: Designs and Analyses,...

Ngày tải lên: 08/04/2014, 12:24

... Med 1989, 170 :20 81 -20 95 Page of (page number not for citation purposes) Virology Journal 20 05, 2: 22 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Frucht DM, Fukao T, Bogdan ... Journal 20 05, 2: 22 SOD activity is defined as the amount of enzyme required to inhibit autoxidation by 50% at 25 °C Splenocytes preparation Proliferation assay was conducted at 12 h, 3, 6, 9, 12 days ... in the design of the study, carried out the work with the mice, assisted in experimental work and drafting of the manuscript Abdel-Moneim conceived the study, designed and carried out the experimental...

Ngày tải lên: 18/06/2014, 22:20

... Med 1989, 170 :20 81 -20 95 Page of (page number not for citation purposes) Virology Journal 20 05, 2: 22 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 Frucht DM, Fukao T, Bogdan ... Journal 20 05, 2: 22 SOD activity is defined as the amount of enzyme required to inhibit autoxidation by 50% at 25 °C Splenocytes preparation Proliferation assay was conducted at 12 h, 3, 6, 9, 12 days ... in the design of the study, carried out the work with the mice, assisted in experimental work and drafting of the manuscript Abdel-Moneim conceived the study, designed and carried out the experimental...

Ngày tải lên: 20/06/2014, 04:20

... Condensed Matter and Materials Physics 20 00, 61:4968-4974 Page of 10 (page number not for citation purposes) Journal of Nanobiotechnology 20 05, 3:6 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 ... seconds, sequential 2- fold dilutions of the solutions were added to cultures of target cells (2 × 105 MT -2 and × 105 cMAGI HIV-1 reporter cells with 0 .2 0.5 multiplicity of infection (m.o.i) of ... used were of the highest quality available cMAGI cells were cultured in DMEM Dulbecco's Modified Eagle Medium (DMEM) (1X) liquid without sodium phosphate and sodium pyruvate The medium contained...

Ngày tải lên: 11/08/2014, 00:22

... demonstrated that disulfide-based Page of compounds are capable of interacting with the cysteine residues of the Zn-binding domains of Junin and TCRV, which leads to cross-linking of the protein and loss ... absorption, unbound virus was removed and washed with phosphate buffered saline (PBS), and DMEM supplemented with 2% FBS and 1% PS was added to the cells The virus was allowed to replicate for days at ... (uncoated, 50 μg/ml) treated TCRV (c, d) , or 25 nm (uncoated, 50 μg/ml) treated TCRV (e, f) with b, d, and f being zoomed-in images of the white squares of a, b, and c Images g and h depict virus and...

Ngày tải lên: 11/08/2014, 00:22

... cycles PDIOX 30 MDH 24 PEX 42 22 RD21A 25 3OA 27 MO1F PR3 27 30 PR4 22 PDF1 .2 27 actin2 23 UBC 27 CBP 28 Figure genes selected2 Semi-quantitative RT PCR analysis of transcript levels of Semi-quantitative ... http://www.biomedcentral.com/1471 -22 29/9/31 are involved, because disease resistance did not directly correlate with camalexin levels in the 24 ecotypes studied [27 ] Indeed, A brassicicola infection of wild type and camalexin-deficient ... no no no AT4G1 626 0.1 yes AT4G21960.1 AT3G48360.1 AT1G 127 80.1 AT1G47 128 .1 AT3G61470.1 AT3G5 620 0.1 AT5G23040 .2 AT1G23310 .2 AT5G 020 20 .2 AT5G28030.1 AT3G48890.1 AT3G51550.1 AT1G61 520 .2 AT3G19930.1...

Ngày tải lên: 12/08/2014, 03:20

... those used with the amylose-agarose flow-through were added to the wells Determination of relative peptide solubility A two-fold serial dilution of peptide in DMSO was performed, and added in duplicate ... coil derived from HTLV-1 TM and substitution of critical amino acid residues within the peptide disrupts coiled coil binding and impairs the biological activity of the peptide [20 -22 ] These findings ... H409 and R416 interact with the side chains of six residues of the coiled coil, but H409 and R416 are not conserved in BLV and of the six interacting coiled coil residues four have diverged and only...

Ngày tải lên: 13/08/2014, 05:21

... peptide synthesis to realize the designed peptide A lot of studies of interaction regarding the designed antimicrobial peptides in vitro and in vivo have been performed Some of the designed antimicrobial ... of studied antimicrobial peptides interacting with membranes The study should enhance the understanding of the predominant specific property of V4 and the mode of action on membranes The study ... Schematic drawing of the investigation of the mechanisms of antimicrobial peptides by rhodamine 6G entrapped LUVs and Rho-PE labeled LUVs 1 02 Figure 6 .2 Interaction of antimicrobial peptides with rhodamine...

Ngày tải lên: 14/09/2015, 21:54

... solution Additional chloroform was then added to prepare a solution with V4 concentration of 0.2mM Required volume of POPG or POPC was mixed with V4 to form lipid/V4 mixture with V4 percentage of 0%, ... properties (density and thickness) of adsorbed layers at a solid-liquid interface to be determined 1 32 When a peptide is introduced into the thin lipid bilayer, the changes of average thickness and average ... and DPPG was dissolved in the mixture of chloroform and methanol (v/v=3:1) with a final lipid concentration of 0 .2 mM All the studied antimicrobial peptides were dissolved in water with high concentrations...

Ngày tải lên: 14/09/2015, 23:32

... aims 20 CHAPTER IN VITRO INTERACTION BETWEEN BURKHOLDERIA PSEUDOMALLEI AND HOST T CELLS AND DENDRITIC CELLS 22 INTRODUCTION 23 MATERIALS AND METHODS 24 Culture and maintenance of cell lines 24 ... involved in the case of live B pseudomallei Enhanced IL -2 production by purified human CD4+ T cells infected with live B pseudomallei Previous observations of B pseudomallei modulation of IL -2 production ... medium with % FCS at a density of 0 .2 x 106 cells/ml Cells were infected with live bacteria at the desired MOI as described above Two hours after infection, 25 0 µg/ml of kanamycin was added to...

Ngày tải lên: 08/11/2015, 16:31

... concentrations of the drug can be achieved as the drug is delivered predominantly to the site of action instead of distributed throughout the whole body Figure 2. 2 Surface-modified nanoparticle as targeted ... Role of stabilizers in nanoparticle synthesis 20 2. 2.3.1 Polyvinyl alcohol (PVA) 20 2. 2.3 .2 Vitamin E TPGS 21 2. 2.3.3 Other stabilizers 22 2. 2.4 Delivery routes and ... 10 2. 2 Nanoparticles in drug delivery 11 2. 2.1 Stages involved in the development of polymeric nanoparticles 12 2 .2. 2 Nanoparticles preparation methods using preformed polymers 17 2. 2.3...

Ngày tải lên: 08/11/2015, 16:31

... waves described above, pres- treatment zone of the shock wave device via corresponding sure waves with different features are used in modern medi- adjustment Shock wave systems are offered with ... Süddeutscher Orthopäden e.V., Nr 328 , April 20 05 17 Also, radial shock waves are used for smoothing the muscles after shock wave therapy Fig 28 – MASTERPULS® MP200, radial shock wave therapy device ... 22 , 23 , 24 – Generation of harmonic oscillations (rod waves) in the impact body: the displacement of the impact body is superimposed by a harmonic oscillation (rod waves) in the impact body 22 ...

Ngày tải lên: 11/05/2016, 20:19

... FEBS 20 03 expressed and purified as described by Bukach et al [19], and Panasenko et al [20 ] Three point mutants of HSP25 with replacements S1 5D ( 1D mutant), S7 7D + S8 1D ( 2D mutant), and S1 5D + ... (2) or 43 °C (3) 10% of pyrene-labeled protein for h at 43 and 60 °C and analyzed polymerization and aggregation induced by the addition of KCl and MgCl2 (Fig 5) As expected, salt addition induced ... on the interaction of HSP25 with partially unfolded actin, we analyzed the effect of different quantities of HSP25 on the salt-induced Fig Effect of HSP25 on the salt-induced aggregation of partially...

Ngày tải lên: 20/02/2014, 23:20