... sets [38,53,54] and in excellent agreement with exper- imental energetics. [55] Binding energies were computed as the dif- ference in energy between the dimer and the sum of the opti- mized energies of the ... O···S interactions. It is worthwhile to consider how the results presented herein might be altered if the model systems were enlarged to more accurately represent the actual protein segments. The CH 3 SH ... Noncovalent Bonds to the Interaction between an Amide and S-Containing Molecules Upendra Adhikari and Steve Scheiner* [a] 1. Introduction Because of its prevalence in proteins, the peptide linkage...
Ngày tải lên: 16/12/2012, 15:21
... are observed at the pachytene stage. CcCac1L began to accumulate at the leptotene and zygotene stage, and decreased after the pachytene stage (Fig. 2A). This expression profile suggests the specific ... mei- otic tissues (Fig. 2E) . Taken together, these results suggest that the interaction between CcLim15 and CcCac1L is related to specific events during the mei- otic prophase. Interaction between ... The BIAcore system enabled us to detect the surface plasmon resonance (SPR), which measures the interaction between a ligand on a detection surface (sensor chip) and a ligand that is injected....
Ngày tải lên: 07/03/2014, 05:20
Quantification of vitamin e and ç oryzanol components in RiceGermandBran
... temperature until ethanol and methanol were removed followed by drying in a freeze-dryer. The dry extracts (extractable phytochemicals) represented 24.8% of the rice germ and 16.3% of the rice bran, respectively. ... respectively. The extract was partitioned between water (200 mL) and dichloromethane 3 times (200 mL ì 3). The aqueous layer and combined dichloromethane extract were rotary evaporated under reduced ... vitamin E and γ-oryzanol was considered nearly complete because vitamin E and γ-oryzanol were not detected in a fifth extraction with 100% ethanol. In the water-dichloromethane partition, there were...
Ngày tải lên: 15/03/2014, 15:33
EUROPEAN COMPETITION LAW ANNUAL 2005: The Interaction between Competition Law and Intellectual Property Law doc
... possibly even of theoretical dependence. 14 In the area of interest here, the concern of modern welfare economics for welfare enhancement through efficient xxx Interaction between Competition Law and ... Enforcement Agencies 463 II Benoợt Durand: Intellectual Property and Merger Control: Review of the Recent Experience under the European Merger Regulation 475 x Contents (A) Ehlermann 06 Prelims ... Article 4. Likewise, by considering only the competitive relationship that exists between the licensor and the licensee on the product market before they conclude their agreement, Article 1(1)(j)(ii)...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo hóa học: "Interaction between High-Level and Low-Level Image Analysis for Semantic Video Object Extraction" docx
Ngày tải lên: 23/06/2014, 01:20
Báo cáo lâm nghiệp:"An examination of the interaction between climate, soil and leaf area index in a Quercus ilex ecosystem" ppt
Ngày tải lên: 08/08/2014, 01:21
Báo cáo khoa học: "Vitamin E and selenium plasma concentrations in weanling pigs under field conditions in Norwegian pig herds" pps
Ngày tải lên: 12/08/2014, 18:21
A study on an example of non-verbal interaction between the presenter and audience for English majored students at the School of Foreign Language
Ngày tải lên: 19/03/2015, 10:37
Interaction between nonlinear parametric and forced oscillations
Ngày tải lên: 08/04/2015, 15:28
Interaction between the forced and parametric excitation with different degrees of smallness
Ngày tải lên: 08/04/2015, 15:28
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... species analyzed, although not in the other KINDs. To investigate the possible involvement of these conserved Leu residues in the interaction between v-KIND and MAP2, we generated EGFP-tagged ... of GST-fused deletions of the CD2-1 region (Fig. 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig. S1C) and endogenous cerebellar v-KIND protein. All ... deter- mine whether a hydrophobic interaction between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins. In conclusion, the present study has clarified the structural...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... convincing evi- dence for the existence of independent entities in such functions was not presented. Loose interaction between the two enzymes enables interactions of an enzyme with other proteins ... curves of cerulean (blue) and citrine (green) represent an average of fluorescence decay data obtained from the cytoplasmic area of the observed cells. The decay curve of separately expressed cerulean–GAPDH ... interaction between the two enzymes was determined by the simultaneous expression of GAPDH–citrine and PGK–cerulean. We expressed three pairs of chimeric proteins to determine FRET signals...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... less than 5% of total activity at pH 6.4). Reported data are the results from one experiment with three replicates representative of at least three experiments; SE of the assays did not exceed ... the sequence represent nucleotides match- ing target sequence). The PCR product was cloned into pET-32b plasmid (Novagen) deleted of the thioredoxin gene, using NdeI and XhoI restriction sites. ... triangles) nmolặmin )1 ặmg protein )1 . Results are from one experiment, representative of three giving similar results. C. Viotti et al. Interaction between plasma membrane H + -ATPase and PPI1 FEBS...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... de novo, are compatible with each other in the CSFV viral genomic replication. To characterize the i nteraction between the CSFV NS5B protein and different sites of the genome, competitive electrophoretic ... six histidines at the C-terminus were engineered to facilitate the purification of NS5B protein. The i nserted regions of all clones were sequenced through d ideoxynucleotide sequen- cing and no c hanges ... coding sequences were initially cloned into the pGEM-T vector (Promega) f rom total C SFV RNA by RT-PCR. These nucleotide sequences were verified. A pair o f primers were designed on either side of...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Functional interaction between RNA helicase II⁄Gua and ribosomal protein L4 pptx
... discarded and the nucleolar pellets were resuspended in NET2 buffer and subjected to immunopre- cipitation as described above. RNAs were isolated from the immunoprecipitate with TRIzol Reagent (Invitrogen). ... (A) Schematic representation of wild-type and mutant forms of human RPL4. The open bar represents regions conserved between human and mouse RPL4. The shaded bar represents nonconserved regions. ... resin as described under Experimental procedures. RNA components were isolated and resolved in a 1.2% agarose- formaldehyde gel and blotted onto a nitrocellulose membrane, which was subjected...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... effects have been reported when bivalent rather than univalent cations were used in measurements of ionic strength dependence (see the Introduction). Hence, the dependence of k 2 of wild-type and ... concentration were used to determine the effect of ionic strength on the activation enthalpy, entropy and free energy. No significant difference was observed between the thermodynamics of the NaCl and MgCl 2 dependencies. Figure ... the magnitude or even sign of each contribution to the measured parameters cannot be precisely determined. It has to be remembered that transition state theory was developed for elementary chemical reaction...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... gast- rins and ApoTf. In the presence of EDTA, no interaction between ApoTf and either Gamide or Ggly was observed (Fig. 2A). Therefore, ferric ions must be present for formation of the complex between ... acids that differ from the naturally occurring sequence are underlined. Peptide Amino acid sequence 1 6 10 18 Gamide ZGPWLEEEEEAYGWMDF NH2 Ggly ZGPWLEEEEEAYGWMDFG OH Ggly(1–11) ZGPWLEEEEEA OH Ggly(12–18) ... Gamide and Ggly bind two ferric ions [11], the iron chelator EDTA was coinjected with ApoTf into the BIAcore channel to determine whether the fer- ric ions were required for the interaction between...
Ngày tải lên: 07/03/2014, 02:20