... sets[38,53,54] and in excellent agreement with exper-imental energetics.[55]Binding energies were computed as the dif-ference in energy between the dimer and the sum of the opti-mized energies of the ... O···S interactions.It is worthwhile to consider how the results presentedherein might be altered if the model systems were enlarged tomore accurately represent the actual protein segments. TheCH3SH ... Noncovalent Bonds to the Interaction between an Amide and S-Containing MoleculesUpendra Adhikari and Steve Scheiner*[a]1. IntroductionBecause of its prevalence in proteins, the peptide linkage...
... are observedat the pachytene stage. CcCac1L began to accumulateat the leptotene and zygotene stage, and decreasedafter the pachytene stage (Fig. 2A). This expressionprofile suggests the specific ... mei-otic tissues (Fig. 2E) . Taken together, these resultssuggest that the interactionbetween CcLim15 and CcCac1L is related to specific events during the mei-otic prophase. Interaction between ... The BIAcore system enabled us to detect thesurface plasmon resonance (SPR), which measures the interaction between a ligand on a detection surface(sensor chip) and a ligand that is injected....
... temperature until ethanol and methanol were removed followed by drying in a freeze-dryer. Thedry extracts (extractable phytochemicals) represented 24.8% of the ricegerm and 16.3% of the rice bran, respectively. ... respectively. The extract waspartitioned between water (200 mL) and dichloromethane 3 times (200mL ì 3). The aqueous layer and combined dichloromethane extractwere rotary evaporated under reduced ... vitamin Eand γ-oryzanol was considered nearly complete because vitamin Eand γ-oryzanol were not detected in a fifth extractionwith 100% ethanol. In the water-dichloromethane partition,there were...
... possibly even of theoretical dependence.14In the area of interest here, the concern ofmodern welfare economics for welfare enhancement through efficientxxx Interaction between Competition Law and ... Enforcement Agencies 463II Benoợt Durand: Intellectual Property and Merger Control: Review of the Recent Experience under theEuropean Merger Regulation 475x Contents(A) Ehlermann 06 Prelims ... Article 4. Likewise,by considering only the competitive relationship that exists between the licensor and the licensee on the product market before they conclude theiragreement, Article 1(1)(j)(ii)...
... species analyzed,although not in the other KINDs. To investigate thepossible involvement of these conserved Leu residuesin the interactionbetween v-KIND and MAP2, wegenerated EGFP-tagged ... ofGST-fused deletions of the CD2-1 region (Fig. 3E) and examined the interactions between bacteriallyexpressed proteins of these subregions (Fig. S1C) and endogenous cerebellar v-KIND protein. All ... deter-mine whether a hydrophobic interactionbetween theLeu residues from v-KIND and MAP2 contributes tothe interactionbetween the two proteins.In conclusion, the present study has clarified thestructural...
... convincing evi-dence for the existence of independent entities in suchfunctions was not presented. Loose interaction between the two enzymes enables interactions of an enzyme withother proteins ... curves ofcerulean (blue) and citrine (green) representan average of fluorescence decay dataobtained from the cytoplasmic area of theobserved cells. The decay curve ofseparately expressed cerulean–GAPDH ... interactionbetween the two enzymes was determined bythe simultaneous expression of GAPDH–citrine and PGK–cerulean. We expressed three pairs of chimericproteins to determine FRET signals...
... less than 5% oftotal activity at pH 6.4). Reported data are the results fromone experiment with three replicates representative of atleast three experiments; SE of the assays did not exceed ... the sequence represent nucleotides match-ing target sequence). The PCR product was cloned intopET-32b plasmid (Novagen) deleted of the thioredoxingene, using NdeI and XhoI restriction sites. ... triangles) nmolặmin)1ặmg protein)1. Resultsare from one experiment, representative of three giving similarresults.C. Viotti et al. Interactionbetween plasma membrane H+-ATPase and PPI1FEBS...
... de novo, are compatible witheach other in the CSFV viral genomic replication.To characterize the i nteraction between the CSFV NS5Bprotein and different sites of the genome, competitiveelectrophoretic ... sixhistidines at the C-terminus were engineered to facilitatethe purification of NS5B protein. The i nserted regions of allclones were sequenced through d ideoxynucleotide sequen-cing and no c hanges ... coding sequences wereinitially cloned into the pGEM-T vector (Promega) f romtotal C SFV RNA by RT-PCR. These nucleotide sequenceswere verified. A pair o f primers were designed on either sideof...
... discarded and the nucleolar pellets wereresuspended in NET2 buffer and subjected to immunopre-cipitation as described above. RNAs were isolated from theimmunoprecipitate with TRIzol Reagent (Invitrogen). ... (A) Schematic representation of wild-type and mutant forms of human RPL4. Theopen bar represents regions conserved between human and mouse RPL4. The shaded bar represents nonconserved regions. ... resin as described under Experimental procedures.RNA components were isolated and resolved in a 1.2% agarose-formaldehyde gel and blotted onto a nitrocellulose membrane,which was subjected...
... effectshave been reported when bivalent rather than univalentcations were used in measurements of ionic strengthdependence (see the Introduction). Hence, the dependenceof k2of wild-type and ... concentration were used to determine the effect ofionic strength on the activation enthalpy, entropy and freeenergy. No significant difference was observed between thethermodynamics of the NaCl and MgCl2dependencies.Figure ... themagnitude or even sign of each contribution to the measuredparameters cannot be precisely determined. It has to beremembered that transition state theory was developed forelementary chemical reaction...
... gast-rins and ApoTf. In the presence of EDTA, no interaction between ApoTf and either Gamide or Gglywas observed (Fig. 2A). Therefore, ferric ions must bepresent for formation of the complex between ... acidsthat differ from the naturally occurring sequence are underlined.Peptide Amino acid sequence1 6 10 18Gamide ZGPWLEEEEEAYGWMDFNH2Ggly ZGPWLEEEEEAYGWMDFGOHGgly(1–11) ZGPWLEEEEEAOHGgly(12–18) ... Gamide and Ggly bind two ferric ions [11],the iron chelator EDTA was coinjected with ApoTfinto the BIAcore channel to determine whether the fer-ric ions were required for the interaction between...