inlets what do i need to know
Báo cáo y học: " GM-CSF and IL-2 as adjuvant enhance the immune effect of protein vaccine against foot-and-mouth disease" pot
... these cytokines enhanced the cell-mediated immunity, which was consistent with their known biological function Antigen specific delayed-type hypersensitivity response Delayed-type hypersensitivity ... Th2 cytokine profile detected by semi-quantitative RT-PCR Cytokines play a dominant role in modulating immune response against infection or in the effectiveness of vaccination Therefore, semi-quantitative ... Biological Sciences, China Agricultural University, Beijing 100193, China 3College of Veterinary Science, Qingdao Agricultural University, Qingdao 266109, China Authors’ contributions CZ carried...
- 10
- 301
- 0
Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... JK, Kaitaniemi P, Koskinen P, Manninen V, et al.: Helsinki Heart Study: primary-prevention trial with gemfibrozil in middle-aged men with dyslipidemia Safety of treatment, changes in risk factors, ... similar effect in the contiguous endothelial cells, which, in combination with the CCR2 decreased expression, could be beneficial with respect to the risk of atherosclerosis considering their ... dehydrogenase; HDL: high-density lipoprotein; MCP-1: monocyte chemotactic protein-1; PIs: protease inhibitors; PPARα: peroxisome proliferator-activated receptor α; RQ: relative quantification; SDF-1:...
- 4
- 375
- 0
Báo cáo khoa học: Hydrogen independent expression of hupSL genes in Thiocapsa roseopersicina BBS pot
... roseopersicina showed similarity to HoxA of 4810 Ra eutropha (53% identity and 66% similarity) and to HupR (45% identity and 61% similarity) of R capsulatus In addition, the putative T roseopersicina ... most similar to HupT and HupUV of Azorhizobium caulinodans (65% similarity and 53% identity for HupT, 78% similarity and 68% identity for HupU, 68% similarity and 56% identity for HupV [17]) Downstream ... were identified The predicted parA gene product showed similarity to the partition protein A (57% similarity to ParA of Actinobacillus actinomycetemcomitans) and Orf154 showed 68% similarity to...
- 10
- 551
- 0
expression of recombinant genes in eukaryotic systems
... (hu-LIF) Leukemia inhibitory factor is a cytokine with pleiotropic functions76; from a structural point of view, it is a medium-sized protein, which is heavily glycosylated on seven potential N-linked ... conditions yielding optimal transfection efficiencies need to be determined empirically Again it is recommendable to transfect several cell pools in parallel Primary Selection If a chimeric antibody ... harvested at different time points following infection with varying MOIs Analysis of recombinant protein production in these samples will indicate how much virus is needed to obtain maximal yield after...
- 405
- 349
- 0
Báo cáo sinh học: "Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" potx
... packaging constraints on lentiviral vectors, minimizing the size of sequences required to enable co-expression is important in maximizing the capacity for therapeutic sequences In addition, efficient ... applications, where a major concern may be minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide ... additional amino acids in this case) to the first gene product might also interfere with the function of a given protein, and again this will have to be determined empirically for each application...
- 16
- 535
- 0
báo cáo hóa học:" Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI" ppt
... packaging constraints on lentiviral vectors, minimizing the size of sequences required to enable co-expression is important in maximizing the capacity for therapeutic sequences In addition, efficient ... applications, where a major concern may be minimizing the risk of insertional mutagenesis In addition to the potential for intracellular mislocalisation of protein, the addition of 2A peptide ... additional amino acids in this case) to the first gene product might also interfere with the function of a given protein, and again this will have to be determined empirically for each application...
- 16
- 200
- 0
Báo cáo y học: " Comparison of the expression of cytokine genes in the bursal tissues of the chickens following challenge with infectious bursal disease viruses of varying virulence" pps
... P: Nitric oxide inhibits Marek’s disease virus replication but is not the single decisive factor in interferon-gamma-mediated viral inhibition Virology 2000, 277:58-65 40 Reed LJMH: A simple method ... up-regulation or downregulation of detected cytokines expression This is in accordance with the pathology and clinical signs of two distinctly virulent IBDV Infection with vvIBDV results in lymphoid ... Th1-cytokines expression during IBDV infection Infection with IBDV resulted in transcriptional changes of mRNA encoding IFN-g, IL-2 and IL-12p40 during the acute phase of the disease Differences in...
- 9
- 387
- 0
Differential expression of cellular genes during a west nile virus infection
... occupied by the ectodomain of an E monomer is pink (domain I) , yellow (domain II) and lilac (domain III) (b) Homodimer arrangement of the E protein on the surface of the flavivirus particle (Zhang ... 1, while lineage viruses are mainly restricted to endemic enzootic infection in Africa (Jia et al., 1999; Lanciotti et al., 2002) Due to antigenic cross-reactivity between different flaviviruses, ... scale Literature Review 1.0 LITERATURE REVIEW 1.1 Introduction to West Nile Virus West Nile virus (WNV) is a mosquito-borne virus that was first isolated and identified as a distinct pathogen in...
- 209
- 215
- 0
Expression of EBV genes in nasopharyngeal carcinoma
... classification of World Health Organization (WHO), NPC is classified into histological types: • Type I: keratinising squamous cell carcinoma (SCC) • Type II: non-keratinising carcinoma • Type III: ... This function is achieved through the binding of EBNA1 to oriP which contains multiple EBNA1 binding sites OriP is a cis-acting element in EBV genome By associated with EBNA1, it enables the viral ... Perhaps it facilitates the transition from latency to lytic cycle (Sinclair et al., 1992) In addition to its involvement in viral genes expression, ZEBRA may interfere with IFNγ signal pathway to...
- 136
- 614
- 0
Báo cáo hóa học: "A mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw" docx
... Germany) sterilized by autoclavation The experiments were carried out with a WIS content of 7% with a total working weight of 1.4 kg To obtain the initially desired WIS content in the bioreactor, the ... compounds inhibit ethanolic fermentation (Almeida et al 2007) S cerevisiae strains with an industrial background are generally more inhibitor tolerant than haploid laboratory strains (Almeida et ... process-like conditions Due to the mutated XR the xylose uptake could be doubled along with a significant reduced xylitol yield, resulting in a substantial increase in the ethanol yield It will be important...
- 8
- 329
- 0
Báo cáo sinh học: "Co-regulation of mouse genes predicts function" pps
... learning to discriminate between positive and negative members of a given class of n-dimensional vectors The SVM works by mapping a given training set into a multi-dimensional space and attempting ... the Toronto area and I hired a medical student, Richard Chang, to dissect mice over the summer to obtain the tissues we were still missing Our bioinformatics collaborators, Brendan Frey and his ... 20,000 to 25,000 mark Mining the resulting data mountain required a sophisticated bioinformatic approach “You have to know what you are looking for and be able to formulate questions mathematically...
- 5
- 277
- 0
Báo cáo lâm nghiệp: "doubling atmospheric CO concentration on 2 matter distribution and CO exchange of 2 yr growth, dry 2 old sweet chestnut trees (Castanea sativa Mill.)" pptx
... shoots) was, during that period, 30% lower in the C0 plants -enriched Dry matter partitioning (Table III) Table III shows that the root biomass of -enriched C0 plants was 69% heavier than roots ... were transferred into an airtight assimilation chamber where environmental conditions were controlled (22/15°C day-night, 15 h photoperiod, 50% RH) Whole plant photosynthesis at 250 jlmol-m- ... leaf thickness In the C0 chest-enriched nut plants, stomatal density and stomatal index were not significantly different from the control Growth pattern (Table 11) The C0 enrichment seemed to shorten...
- 3
- 237
- 0
Báo cáo y học: " Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplicatio" potx
... window interval In addition, only 45 nucleotide binding site-encoding genes were identified in Br, suggesting that the total number of nucleotide binding site-encoding genes in the Br genome is likely ... duplication after the eudicot divergence and additional rounds of polyploidization following the divergence of Arabidopsis from its common ancestor with cotton [5] In this study, we refined inferences ... Brassica ancestor, accelerating gene loss in this triplicated ancestor compared to the Arabidopsis-Brassica common ancestor Alternatively, differences in the life cycles of Brassica progenitors might...
- 18
- 487
- 0
báo cáo khoa học: " Molecular characterisation and genetic mapping of candidate genes for qualitative disease resistance in perennial ryegrass (Lolium perenne L.)" doc
... allelic diversity within NBS domain is low compared to the highly variable LRR domain Previous studies identified significantly non-random chromosomal distributions of NBS-containing sequences ... bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery ... bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery LpEST from bioinformatic discovery...
- 22
- 446
- 0
báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... stresses is challenging; therefore, it is important to identify candidate reference genes that are subject to only minimal regulation during an individual experiment, permitting accurate transcriptional ... then analyzed to verify the specificity of each amplification reaction; the dissociation curve was obtained by heating the amplicon from 60°C to 95°C (See additional file 1: Dissociation curve data) ... necessarily identify the putative Arabidopsis ortholog Closest Arabidopsis homologs were identified using TAIR BLAST 2.2.8 * The PCR efficiency was determined with LinReg software [23] † Terrier...
- 11
- 376
- 0
Cloning, characterisation and functional analysis of horseshoe crab c reactive proteins
... factor lippopolysaccharide binding protein lippopolysaccharide matrix assisted laser desorption ionization minimum-inhibitory concentration 3-(N-morpholino) propanesulfonic acid polyacrylamide ... the interactions of rCRP-2 that are important for antimicrobial activity 95 C-terminal α-helix of rCRP-2 is critical for antimicrobial activity 98 Amphipathic profile of the C-terminal portion ... expression and purification efficiencies of recombinant CRP-1 and -2 in different host systems 60 Examples of endotoxin-binding proteins which interact with Lipid A 109 4.1 viii Summary The horseshoe...
- 129
- 467
- 0
Báo cáo y học: "otifs and cis-regulatory modules mediating the expression of genes co-expressed in presynaptic neurons" ppsx
... functioned as cis-regulatory elements We then identified 16 transcription factor binding motifs that were enriched in intergenic MCEs in the vicinity of these nine genes We devised a computational ... Berman BP, Nibu Y, Pfeiffer BD, Tomancak P, Celniker SE, Levine M, Rubin GM, Eisen MB: Exploiting transcription factor binding site clustering to identify cis-regulatory modules involved in pattern ... additional epigenetic factors such as nucleosome positioning, DNA methylation and a number of histone modifications [52-54] Such epigenetic marks play critical roles in gene regulation in higher...
- 18
- 320
- 0
Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... PROSITE prion protein signature motif (PS00291), which is held to be a signature of PrPs in vertebrates This so-called hydrophobic region is rich in small amino acids (Gly, Ala) and is included in ... a region with similarities to viral fusion peptides and reactive loops of serpins [28] Additional conserved sequence motifs and amino acid positions in zebrafish PrP2 and tetrapod PrPs are included ... Transcripts of prp1 started to be detected by 48 hpf in cranial ganglia including the trigeminal ganglia and their projections (Fig 4A,B,D,E) The hybridization signal was maintained in the ganglia...
- 14
- 547
- 0
Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... expression at h poststimulation was recorded in both head kidney and liver This indicates that the gene is inducible by LPS stimulation Administration of endotoxin induced IL-10 production in mice, ... motifs, in comparison to five in human IL-10, in the 3¢-UTR Whether this difference in the number of instability motifs affects translation needs to be investigated in future studies LPS induction ... shown in italics The deduced amino acid sequence is given below the nucleotides The motifs associated with mRNA instability are shown in bold and the polyadenylation signal in bold italics The stop...
- 8
- 584
- 0
Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... activities were inhibited by 10 mM azide, but ADPase activity was more sensitive to azide inhibition than ATP hydrolysis and inhibition in DISCUSSION The existence of a cell surface ATPase in the bile ... investigation The function of the liver ecto-ATPDase is not established While its localization in the bile canaliculi is suggestive of its involvement in bile acid secretion, experimental evidence ... sensitive to azide inhibition than its ATPase activity at either pH 7.4 or pH 6.4 (Fig 3) At pH 7.4, mM azide inhibited ADP hydrolysis by 70% whereas ATP hydrolysis was inhibited by only 10% Inhibition...
- 10
- 694
- 0
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25