in vivo functions of heterotrimeric g proteins
Báo cáo y học: "Could increased axial wall stress be responsible for the development of atheroma in the proximal segment of myocardial bridges?" pot
Ngày tải lên :
13/08/2014, 16:21
... tunneled segment pulls axially at that segment during systole, due to strong morphologic changes in the bridge region during the heart contraction (e .g. , deeper dipping of the tunneled segment into ... Kearney P, G rge G, Haude M, Meyer J: Comparison of intravascular ultrasound and angiography in the assessment of myocardial bridging Circulation 1994, 89(4):1725-1732 Ge J, Erbel R, Gorge G, Haude ... pushing in the entrance cone of the bridge; F2 is the force generated by the drag of the blood in the tunneled segment; F3 is due to the pressure pushing in upstream direction in the exit cone of...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc
Ngày tải lên :
08/03/2014, 08:20
... anti-CREB Ig and equal protein loading is shown by probing the same blot with GAPDH Ig mediated through various protein kinase and monomeric GTP-binding protein signaling pathways including MAP kinases ... Cyr61/CCN1 gene is a downstream target of S1P signaling in primary cultures of SMCs and its regulation occurs at the promoter level We investigated the nature of the intracellular signaling pathways ... continuous region of high homology between human and mouse with conserved transcription factor-binding sites Using a combination of cis-element mutagenesis and the expression of dominantnegative...
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unsicker - cell signaling and growth factors in development
Ngày tải lên :
03/04/2014, 12:05
... by law Printed in the Federal Republic of Germany Printed on acid-free paper Typesetting pagina GmbH, Tübingen Printing betz-druck GmbH, Darmstadt Bookbinding J Schäffer GmbH i G. , Grünstadt ... Regulation of Intracellular Signaling Networks 809 Regulation of TGF-b signaling by EGF Signaling 810 Calcium Signaling and the Mitochondrial Apoptosis Pathway 810 Developmental Modulation of Growth ... loss of Notch1 signaling in the epidermis of mouse skin de-represses Wnt signaling and leads to increased levels of free, signaling competent b-catenin [99] In contrast, forced Notch1 signaling in...
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Báo cáo y học: "The NFI-Regulome Database: A tool for annotation and analysis of control regions of genes regulated by Nuclear Factor I transcription factors" pdf
Ngày tải lên :
10/08/2014, 09:22
... Figure Graph View of genes containing AP-4 binding sites, aligned by ATG as The height of the bar indicates the number of binding sites of each type within the bin of 1000 bp Bins can be adjusted in ... be determined Future growth and management of the database Figure Table view of genes returned by query for genes containing AP-4 binding sites The Gene Name, location of the binding site, TF ... displays Binding sites for TFs can be displayed in a number of ways Selecting and submitting a TF family or gene returns a display of the location of binding sites on the single or multiple genes...
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Báo cáo y học: "Science review: Redox and oxygen-sensitive transcription factors in the regulation of oxidant-mediated lung injury: κ role for nuclear factor-κ" pdf
Ngày tải lên :
12/08/2014, 19:21
... Figure SCHEMATIC DIAGRAM OF NFκB ACTIVATION CIRCUITS OXYGEN-SIGNALLING IN HYPEROXIA nγ-GCS GSSG BSO BCNU ↑ GSH γ-GCS 2GSH ? NF-κB Activation ROOH ROOH NAC PDTC ROS ↑ GSSG ROS NF-κB DNA Binding ... leads to increasing intracellular stores of GSSG, a potent inhibitor of NF-κB transcription factor DNA binding The pathway leading to the formation of GSH by the action of γ-glutamylcysteine synthetase ... therapeutic strategies 484 The role of oxidative stress, cytokines and other inflammatory signals in regulating the NF-κB signal transduction pathways in mediating oxidant-induced lung injury and disease...
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Báo cáo y học: "Science review: Redox and oxygen-sensitive transcription factors in the regulation of oxidant-mediated lung injury: α role for hypoxia-inducible factor-1α" pptx
Ngày tải lên :
12/08/2014, 19:21
... to indicate HIF-1 as a possible regulator of the evolution and propagation of the inflammatory process The rate of transcription of several genes encoding proteins involved in oxygen and energy ... implicates VEGF as a potential mediator of increased pulmonary vascular permeability in this model of acute lung injury Further elaborating on the mechanisms involving HIF-1 in regulating the inflammatory ... The involvement of L-γ-glutamyl-L-cysteinyl-glycine (glutathione/GSH) in the mechanism of redox signaling mediating MAPKp38-dependent regulation of pro-inflammatory cytokine production Biochem...
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Báo cáo y học: "Genome-wide identification of novel expression signatures reveal distinct patterns and prevalence of binding motifs for p53, nuclear factor-κB and other signal transcription factors in head and neck squamous cell carcinoma" docx
Ngày tải lên :
14/08/2014, 07:21
... -TCCATTGGAACATCTCTGGACATCAGCTTGGGAGGAGGGGCTGAGGAGGG- 3’ Rat ARID1A 5’ 5’ 3’ 5’ +45 (EGR1) (p53) +74 –AGCGGAGCCTCCACCGCCCCCCTCATTCCCAGGCAAGGGCTTGGGGGGAA-AGCGGAGCCTCCACCGCCCCCCTCATTCCCAGGCAAGGGCTTGGGGGGAA-AGCGGAGCCTCCACCGCCCCCCTCATTCCCAGGCAAGGGCTTGGGGGGAA-AGCGGAGCCTCCACCGCCCCCCTCATTCCCAGGCAAGGGCTTGGGGGGAA- ... binding sites κ ICAM1 5’ 5’ 5’ 5’ -173 (p65/cRel) -158 –ATTGCTTTAGCTTGGAAATTCCGGAGCTGAAGCGGCCAGCGAGGGAGGAT-ATTGCTTTAGCTTGGAAATTCCGGAGCTGAAGCGGCCAGCGAGGGAGGAT-ATTACTTCAGTTTGGAAATTCCTAGATCGCAGGGGCCAGCGAGGCAGGAC-ATTACTTCAGTTTGGAAATTCCTGGGTCGCAGGGGCCAGCGAGGCAGGAC- ... p53 binding sites CPS1 -105 (p53) -85 5’ -CCCA-TGGAACATCTCTGGACATTACCTCAGGAGGAGGGGTTAAGAGAAG- 3’ Human 5’ -TCCATTGGAACATCTCTGGACATCAGCTTGGGAGGAGGGGCTAAGGAAAG- 3’ Mouse 5’ -TCCATTGGAACATCTCTGGACATCAGCTTGGGAGGAGGGGCTGAGGAGGG-...
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Tài liệu Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007 ppt
Ngày tải lên :
14/02/2014, 08:20
... showing location of the two sampling sites on the North Fork of the Shenandoah River, Virginia Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of ... Furlong, E.T., and Holmes, J., 2008, Investigation of organic chemicals potentially responsible for mortality and intersex in fish of the North Fork of the Shenandoah River, Virginia, during spring ... observations of intersex in fish have been increasing in regularity in the Shenandoah River and Potomac River basins in Virginia (Blazer and others, 2007) The fish kills and observations of intersex...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
Ngày tải lên :
16/02/2014, 09:20
... GCTGCCTTTGTTATTTGTAAGCTTCAG GGAAACTTCCTGTCTCATCCAGTG GGAACACACAGAGGGGATGATA CTTCAACACGCACAAAGCAC TGCCACCTTTTCCATCATACA CTGCTTTTCTGGGGACTTCA TGAAGTCCCCAGAAAAGCAG GTGCTTTGTGCGTGTTGAAG TGTATGATGGAAAAGGTGGCA GGAACACACAGAGGGGATGATA ... CCATGTCTGCAGATGGTCGAGG GGACTGACATTGCTCCAGAGC GCTTCAGTGACTCAGAAATTGG GTCCAGAATATTCAGCCTTTCACC CTCCCTCAAACAAACCAGAGTC CACTGGATGAGACAGGAAGTT CTTCTCCAGGACAGTCCAAAGAGTC CTGGATTGAAGCGCCCTCGGTTAATC GCTGCCTTTGTTATTTGTAAGCTTCAG ... Oligo dG Adapter dT Adapter primer AGTGAGATGGATACAGGTGCTAAAC TCTGGACCTCAGACATGAACTTACT TGTCAGTCCTCTTTAATGCT AATGGTATCCTGTTTGGCTCAG GGTTGTAATTGTACACGGTAGTC CGGTAGTCAGGAAATCAATGCC CCATGTCTGCAGATGGTCGAGG...
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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
Ngày tải lên :
16/02/2014, 09:20
... DDLQGSVEAQ-CGLVLNGQGGVIRRG -*.: ::: : : : * **:: Alpha Beta Gamma Epsilon Delta IPINKDNLFGGV-VNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGG LMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGG ... Gamma Epsilon Delta HPGWPGQRQ -SQESGLLHTHRGLPHQLSG-LDP -RRDY -RRHEDLLHGP-HA HPWGQRQRQEVGSEAGSLLPQPRAALPQLSG-LDP -RRDYHSVRRPDVLLHSAHHG QQAWPGRQSQEGAGLPSHHGRPAGLLPHLSG-LEAGAVSARRDAY RRSDLLLPHAHAL ... Beta Gamma Epsilon Delta VLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETE VLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETE VLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAE...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
Ngày tải lên :
16/02/2014, 14:20
... myristoylation interferes with binding of the signal recognition particle, resulting in a part of NADH-cytochrome b5 reductase escaping the endoplasmic reticulum insertion pathway and relocating to the ... apoptosis In the extrinsic pathway, binding of death ligands to their respective receptors recruit adaptor proteins, such as Fas-associated death domain protein, which in turn bind and aggregate FEBS ... ⁄ PAGE The gel was fixed in 40% methanol ⁄ 10% acetic acid, and stained with SYPRO Ruby Protein Gel Stain (Invitrogen) according to the manufacturer’s protocol Bands containing JSP1-wt or G2 A...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... E52H-fwd: 5¢-GCCTGCTGAC CCACCCCGTCGAGAAGTGGCGC-3¢ E52H-rev: 5¢-GC GCCACTTCTCGACGGGGTGGGTCAGCAGGC-3¢ E52Qfwd: 5¢-GCCTGCTGACCCAGCCCGTCGAGAAGTGG CGC-3¢ E52Q-rev: 5¢-GCGCCACTTCTCGACGGGCTG GGTCAGCAGGC-3¢ ... Y70W-fwd: 5¢-CTGCTGGAGCT GATGTGGAAAGATCCCAAGAAG-3¢ Y70W-rev: 5¢-CTT CTTGGGATCTTTCCACATCAGCTCCAGCAG-3¢ Q81Nfwd: 5¢-TGGGCCATGCCCTTTAACAGTTATGTCACG CTG-3¢ Q81N-rev: 5¢-CAGCGTGACATAACTGTTAAA GGGCATGGCCCA-3¢ ... 5¢-GCTAAAAATAA R105HTGGAGCACTCCATTTTTAGCGCTCGC-3¢ rev: 5¢-GCGAGCGCTAAAAATGGAGTGCTCCATTAT TTTTAGC-3¢ R105K-fwd: 5¢-GCTAAAAATAATGGAG AAATCCATTTTTAGCGCTCGC-3¢ R105K-rev: 5¢-GCG AGCGCTAAAAATGGATTTCTCCATTATTTTTAGC-3¢...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
Ngày tải lên :
21/02/2014, 03:20
... 5¢-ATGGTACCGGGCTTTAAGGGTTCCCG-3¢ 5¢-ATGGTACCGGAAGGAAAGCAGAGCCC-3¢ 5¢-ATGGTACCTGGTGATCCAGGGCTTGC-3¢ 5¢-CCGTTCCGAGCTCCGAGCAC-3¢ 5¢-ATGAGCTCGGGAACCCTTAAAGCCCG-3¢ 5¢-ATGAGCTCTGCTTTCCTTCCGGGGA-3¢ 5¢-ATTGAGCTCACCAGGAGGGCAGGAGG-3¢ ... 5¢-ATGAGCTCTGCTTTCCTTCCGGGGA-3¢ 5¢-ATTGAGCTCACCAGGAGGGCAGGAGG-3¢ 5¢-GACACCTGTCGGTAACCCTTAAAGCC-3¢ 5¢-CGGAGTCGCCTAAGGAGAGATGGAGA-3¢ 5¢-AGGGTTCCCGATCGGTGTCTGAGAGA-3¢ 5¢-TTTTCTCTTTCCCCGTAAGGAAAGCA-3¢ Ó FEBS 2002 4526 T ... al [34] The DNA fragments containing the putative Etsbinding sequence of the 5¢-flanking region of the mouse a3 integrin gene were synthesized; 5¢-TTTTCTCTTTCCCCG GAAGGAAAGCAGAG-3¢ (wild-type) and...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
Ngày tải lên :
08/03/2014, 16:20
... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 41 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of bacterialphage ... 5F (5¢-TGAAGAGTTT GATCATGGCT-3¢) and 1540R (5¢-AAGGAGGTGAT CCAACCGCA-3¢) numbered according to the E coli 16S rRNA sequence were used The PCR product was purified and ligated into the p-GEM-T Easy ... N-D-AAase gene (Eur J Biochem 269) 4877 37 Thompson, J.D., Higgins, D .G & Gibson, T.J (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,...
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Báo cáo khoa học: The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors pdf
Ngày tải lên :
15/03/2014, 00:20
... CGCACGCGTGGCACAGTGATGTAAATC CGCGAGCTCTTGCTCCCTGGGGGCCTG CGCACGCGTGGCACAGTGATGTAAATC CGCGAGCTCCCAGGGCCCTTGAGAC CGCACGCGTCCTGGGGGCAGTACA CGCGAGCTCCAGGGACCCTGCCAG CGCACGCGTCCTGGGGGCAGTACA S AS S AS ... Oligonucleotide sequences (5¢ fi 3¢) Orientation CGCGAGCTCCACATAGACTTTTCCCTT CGCACGCGTGGCACAGTGATGTAAATC CGCGAGCTCCCAGGGCCCTTGAGAC CGCACGCGTGGCACAGTGATGTAAATC CGCGAGCTCCAGGGACCCTGCCAG CGCACGCGTGGCACAGTGATGTAAATC ... T242 GATA ()454 ⁄ )449) GATA CTGCCATGGCCCCTCCCCAAGAGCAAA CGGCAAACACAAGCCAAGGTTGTTGTC CGGCAAACAGTATCGTATGTTGTTGTC TCCAGGGCCCTTGAGACCCTTGGTCATTTC TCCAGGGCCAGTAAGACCAGTAGTCATTTC CCCCTGATCCTTGTAGTGTCTAGT...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
Ngày tải lên :
16/03/2014, 04:20
... (5¢-CAGTGACCCCAGAC GGGGTCGACTTC-3¢) and DH974 (5¢-GGCTGGTCGTC GCCTCGGCAACAGCGGGTTCCTCCTTC-3¢) for pig CPT1B, and DH977 (5¢-CGGTGACCCCAGAAGGGGT CGACTTC-3¢) and DH978 (5¢-GAAGTCGACCCCTTCTG GGGTCAC CG-3¢) ... DH673 (5¢-AGCTGAATTC ATGGCGGAAGCTCACCAG-3¢) and DH803 (5¢-TCCA CCCATGGTAGCAGAGAAGCAGCTTAAGGGTTTGG CGGA-3¢) The PCR reaction yielded a 422-bp product, in which an EcoRI site (in bold in the forward ... QuickChange Site-Directed Mutagenesis Kit (Stratagene) The primers used were DH801 (5¢-TTCTTCCGCCA AACCCTTAAGCTGCTGCTTTCCTAC-3¢) and DH802 (5¢-GTAGGAAAGCAGCAGCTTAAGGGTTTGGCGGA AGAA-3¢) Using this...
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Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx
Ngày tải lên :
23/03/2014, 06:20
... the N-termini of each oligopeptide chain Prominent members of the quinoxaline-group of chromodepsipeptides Quinoxalines Quinolines O O N MeS S O O O N H N N H H N N H O N O N O echinomycin (macrolactone) ... ring (Cy8 ⁄ 4) or to eight residue rings By contrast, GrsB T-TE is capable of trimerizing pentapeptidyl-SNAC substrates to form 15-residue rings It was suggested that the size of the resulting ... chromophore quinoxaline-2-carboxylic acid (QX), inhibiting transcription and replication, which has led to the progression of echinomycin into clinical antitumor trials The group of quinoline-chromodepsipeptides...
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Báo cáo Y học: Molecular interactions between nuclear factor kB (NF-kB) transcription factors and a PNA– DNA chimera mimicking NF-kB binding sites doc
Ngày tải lên :
24/03/2014, 04:21
... inhibitors of binding of NF-kB p52 and p50 to HIV-1 LTR sequences, not efficiently inhibit the binding of the same factors to the palindromic GGGGATTCCCCT NF-kB IgK DNA sequences This result is of some ... target molecules A total of mg of nuclear factors were incubated for 20 in binding buffer in the absence (–) or in the presence of 50–200 ng of competitor molecules, as indicated After this incubation ... protein–DNA interactions observed q FEBS 2001 Binding of NF-kB proteins to PNA –DNA chimeras (Eur J Biochem 268) 6073 Fig Effects of increasing amounts of DNA– DNA and PDP–PDP hybrids carrying...
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Báo cáo khoa học: NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon ¨ Methanosarcina mazei Go1 pot
Ngày tải lên :
29/03/2014, 21:20
... AAACGTTGCTGACGTGCACAC CGATATTCTCAAGCCCAAGCC TGATGGAGTCTGGGTTGGAAG AGCCAGATTGATTATGGCCGC ATTCGGACACCCTTGAAAGGG ATAGACAAGTCCGCAGTCCCC 4409 Role of NrpRII in M mazei K Weidenbach et al extracted using ... 5¢-GTTTGAAGCTTTTATAAAAGACCCATAC; Mm1028 His.for 5¢-GGTTGACATATGAGCGAATC ⁄ Mm1028 His rev 5¢-GAAGCTTCTTATAAAAGCCCC; and Mm 1772 His.for 5¢-GGTGATATCATATGGTAGAAGTCG ⁄ Mm 1772 His.rev 5¢-GAAGAAAGCTTTAGAGGATAATCTCG) ... time using 300 lL of protein-binding buffer (see above) Potentially DNA-bound proteins were subsequently eluted step-by-step using 200 lL of binding buffer containing increasing amounts of NaCl...
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