in vivo assessment of neural activity via neurovascular coupling

... number of dose-contributing catheters increases Therefore, the catheter contribution index increases with the number of contributing catheters In case of a single contributing catheter, IP = In case ... 5.4 mm and of interstitial edema (T2-w images) -5 ± 6.0 mm in plane, indicating a shift of the irradiation effect volume against the corresponding tolerance dose volume in the direction of the catheter ... source of error in the treatment planning procedure, especially for catheters in oblique direction, since correct placement of the starting point of the catheter is dependent on conspicuity of the...

Ngày tải lên: 09/08/2014, 09:21

... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... to oleic acid and H+-ATPase hydrolytic activity in plasma membrane vesicles of U maydis Specific activity in lmol PiÆmin)1Æmg)1 protein Line generated by linear regression (r ¼ 0.980) curve for ... (data not shown) Changes in activity were observed to be the result of an increase in Vmax with little changes in affinity for MgATP Changes in Vmax correlated with increases in À1 18 : 2/18 : ratio...

Ngày tải lên: 08/03/2014, 22:20

... reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor cells (Figure 3B) In the more advanced 30 weeks tumors the staining ... analysis Page of 13 revealed elevated htid expression in 81% of the luminal A type tumors (Figure 1A), in 20% of the cases of the luminal B type over expressing ErbB-2 and in 18% of tumors diagnosed ... characterized by positive staining ranging from moderate to intense staining In the context of the potential association of htid expression with the biology of human breast cancers of different subtype...

Ngày tải lên: 18/06/2014, 16:20

... added into microwells and incubated at room temperature for 30 min, followed by three PBS washes Blocking for non-specific binding was performed by adding 100 lL of 3% bovine serum albumin (BSA) ... that GNPs are capable of inducing an antibody response in mice, which indicates that factors other than cytotoxicity may be involved and complicates in vivo application of GNPs [16] The current ... albumin (BSA) and incubating for 60 at room temperature, followed by three PBS washes Binding was performed by adding 100 lL of diluted antiserum into microwells and incubating for h at room...

Ngày tải lên: 22/06/2014, 00:20

... be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of DCs which should be superior to in vitro loading of DCs with tumor ... mobilization of DCs using Flt3 ligand, local injection of chemokines such as macrophage inflammatory protein (MIP)-3β, use of DNA vaccines containing bacterial CpG motifs and the use of topical ... expanded in vitro and primed in vivo via intratumoral injection of DCs In the present study, the anti-tumor effectiveness of a novel anti-cancer treatment via photoimmunotherapy uitilizing the combination...

Ngày tải lên: 28/11/2015, 13:45

... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5.  Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at the equivalent...

Ngày tải lên: 23/04/2013, 21:38

... are in agreement with those of a recent work in which GatY has been identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that ... unfolded proteins with the heat-shock protein GroEL Nature 336, 254–257 25 Lorimer, G.H (1996) A quantitative assessment of the role of the chaperonin proteins in protein folding in vivo FASEB J ... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this...

Ngày tải lên: 22/02/2014, 07:20

... having heard most of the sessions, some inmates with concentration difficulties who fell into incorrectly filling in the survey after almost two hours of explications, or the presence of inmates ... that we can find within a facility (39 in our case) and the difficulty of repeating coordinated activities of this type in the future, the idea was discarded We also considered doing the survey ... survey in three months, extending our experience in time and even intervening in the design of diabetic menus for the concerned inmates so that their specific needs could be met, regarding both...

Ngày tải lên: 05/03/2014, 22:21

... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine ⁄ water ⁄ NaOH ⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...

Ngày tải lên: 06/03/2014, 11:20

... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments ... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution...

Ngày tải lên: 07/03/2014, 16:20

... addition, an increased number of eosinophils were observed in lung interstitium of sensitized mice, confirming the eosinophilic response following Der p challenge (Fig 4) The tissue levels of radioactivity ... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... mimicking inhalation of the natural allergen The fact that i.t instillation of [75Se]Der p led to deposition of radioactivity in alveoli and bronchioli with no detectable allergen remaining in the...

Ngày tải lên: 07/03/2014, 21:20

... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... or light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...

Ngày tải lên: 07/03/2014, 21:20

... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased ... reproduced in lung, which can explain in part the conflicting results found in adults and children given b-carotene or vitamin A [29] Moreover, in lung of rats injected with high-dose of vitamin A over ... cyclin dependent kinase inhibitor that is a critical target of p53 in facilitating G1 arrest, we also studied the expression of p21WAF1/CIP1, and found that it is increased in chronic-vitamin...

Ngày tải lên: 08/03/2014, 08:20

... Goal of Training Session Three The Training Setting Summary of the Training Activities Reminders Session Three Training Activities (Estimated Time: 3.5 Hours) Preparation of the SPs for Training ... Guidelines for Training Training Principles Training Manuals Chapter Summary Looking Ahead 151 151 152 158 161 161 Chapter Training Session One: Familiarization With the Case The Goal of Training ... Dress Rehearsal (Clinician Verification of SPs’ Authenticity) The Goal of Training Session Four The Training Setting Summary of the Training Activities Reminders Session Four Training Activities (Estimated...

Ngày tải lên: 08/03/2014, 16:20

... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation ... the in vitro and in vivo investigations of miRNA production patterns Second, noninvasive monitoring of miRNAs provides useful information of the possible role of miRNAs involving cellular developments...

Ngày tải lên: 16/03/2014, 01:20

... determination of the amino-terminal amino acid sequence of purified protein was attempted, but no information was obtained, probably due to the protein modification Therefore, the purified protein ... enzymatic activity of TGase Hydrolysing activity of GTP was also found in the purified PpTGase protein as in the case of TGase Mammalian TGase has been shown to contribute to molecular events underlying ... hydrolytic activity, no region homologous with the PLC-interacting region has been found Whether the hydrolysing activity of GTP of PpTGase is related to certain cellular signalling in the slime...

Ngày tải lên: 17/03/2014, 23:20

... early warning system that could identify problems of calibration in a rating For an exposition of discriminatory power measures in the context of the assessment of performance of a rating source ... credit assessment institutions, but also other credit quality assessment sources, including the in- house credit assessment systems of national central banks, the internal ratings-based systems of ... rating of “A”, as issued by the major international rating agencies In its assessment of the credit quality of collateral, the ECB has always taken into account, inter alia, available ratings...

Ngày tải lên: 22/03/2014, 20:20

... conservation of the tryptophan in binding site II and an aromatic residue (mainly tryptophan or tyrosine) in binding site I [22] We have shown that the tryptophan residue involved in binding site ... D2 domain and D(495–535) in the D3 domain This result is in agreement with our previous studies using truncated SSIII isoforms, showing the importance of the D23 domain in starch binding and ... between the N- and C-terminal domains of SSIII in vitro Mapping of the CD-binding region in the N-terminal SBDs In order to identify the SBD region required for the SBD–CD interaction, we performed...

Ngày tải lên: 22/03/2014, 21:20

... the incentive objective can be achieved by combining the reduction of the number of pollutants charged with an increase of charge rates for the remaining parameters, generating a stable flow of ... Yearbook of Armenia, 2003 3.2 Institutional Framework Three government agencies are primarily involved in administering the system of economic instruments for environmental protection in RA The Ministry ... constantly increased from 0.05% in 1998 to 0.09% in 2003 In an international comparison, the share of the overall state environmental expenditure in the GDP in Armenia remains low: 0.25% in 2003...

Ngày tải lên: 23/03/2014, 00:20

... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... reproduced in in vitro conditions if nNOS digestion by calpain is carried out in the presence of HSP90 [27] This finding can also explain the large extent of digestion of eNOS in aorta, in which, in association ... M Averna et al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material...

Ngày tải lên: 30/03/2014, 04:20