... the stem ofthe prosthesis is custom made for each individual patient, during the operation [27] After reaming ofthe femoral cavity, a 3D imprint ofthe cavity is made inthe form of a silicone ... frequencies The graph change in shape and position is more important at the beginning ofthe insertion when the stem displacement is important as well At the end ofthe insertion, when the resistance ... *The circle indicates the place ofthe measuring hardware (presented inthe left picture) behind the transparent wall A member ofthe surgical team holds the shaker during the insertion procedure...
... previous work, we studied the stability and toxicity of PANs, and further investigated the sustained release behavior invivoof drug loading in PANs PA was firstly synthesized and characterized ... dose of 200 mg/kg Invivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma levels ... for the clearance of NPs, thus the reducing rate of MPS uptake could be considered as the best strategy for prolonging the circulation of NPs (Li & Huang, 2008) Therefore, PA/EPI prepared in this...
... powders were injected with Argon as the carrier gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum ... cleaned of soft tissue, and fixed in 70% ethanol The femur bone was trimmed above and below the ends ofthe implant, cleaned of soft-tissue, and fixed in 70% alcohol The specimen was further dehydrated ... rabbit and included inthe SEM analysis The details of this invivo rabbit model have been described previously (Figure 2) [22,28] The appropriate experimental implant was press-fit into the intramedullary...
... Presence of GNPs in Organs The acute symptoms and eventual death of mice receiving GNPs indicated that the injected GNPs might damage major organs Inthe tissues samples stained with haematoxylin and ... Starting from day 14, mice injected with 8–37 nm GNPs showed a significantly camel-like back and crooked spine The majority of mice in these groups died before the end ofthe fourth week The median ... performed to measure the cytotoxicity of GNPs in cultured Hela cells The viability of cells exceeded 80% at the highest concentration of GNP (0.4 mM), indicating that regardless of their size, all...
... possible for their spores to germinate without being inhibited by the presence of bile salts inthe small intestine It could also be concluded that among the six strains the tolerance of spores ... through the stomach of a pig The fact that the viable counts of most ofthe six Bacillus strains did not decrease after h of incubation suggests that a reasonable percentage of these Bacillus strains, ... originally obtained from the fresh faeces of healthy piglets The size ofthe inhibitory zone for the six selected isolates against the four indicator pathogens is shown in Table An example of a plate...
... model of autoimmunity Transient autoimmunity can be induced in mice by the administration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin inthe plasma within the ... administered during the most active stage of disease [29] The discrepancy between the two studies could be due to a number of factors, including the longevity ofthe injected protein and the level of free ... ofthe DNase I present inthe supernatants purified as in (b) The activity was determined inthe presence ofthe normal DNA-MG assay buffer, described in Materials and methods, or following the...
... phenytoin solution administered through the medial lumen and the TPN administered through the distal lumen Inthe final phase, the phenytoin was administered through the proximal lumen and the TPN ... catheters inthe pediatric population, and especially inthe pediatric intensive care setting, are commonly used for the administration of intravenous fluids, drugs, chemotherapeutic agents, inotropic ... the intravascular space, the proximal lumen may be close to the site of entry ofthe vein or even outside the vein This increases the possibility of extravasation if the catheter is accidentally...
... during the inspiratory phase inthein vitro and invivo studies The Performer, the Hygroster, or the Hygrobac was placed between the Y piece ofthe ventilator circuit and the test lung or the ... proportional to the dryness ofthe gas, and so the difference in temperature between the dry and wet probe is related to the dryness ofthe gas [19] At the start ofthe measurements, we inserted the two ... variations We used this offset (in the order of 0.1–0.2°C) to correct the measurements obtained during the study In each condition, the average of three or four readings from the wet and dry probe...
... http://www.retrovirology.com/content/1/1/33 tion The data inthe right side of panel A indicate that Cterminal deletions of GagPol extending into the connection domain result in an 85% or greater decrease inthe initiation of reverse ... role ofthe RT thumb domain in GagPol in tRNALys3 viral packaging tRNALys3 incorpora- tion into HIV-1 is not affected by deletion oftheIN domain in GagPol, nor by further deletion ofthe RNaseH ... similarly invivo is not known Alternatively, the RT connection domain may undergo interactions with Gag that may result in placing the tRNALys3 bound to the thumb domain in RT closer to either NC in...
... PREFACE The thesis is submitted for the degree of Master of Engineering inthe Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor ... fall off and interact with the surrounding medium The falling of TCP then created voids within the polymer, thus exposing their surfaces to hydrolytic attack and weakening the overall structure of ... concentric rings (lamellae) of matrix Between the rings of matrix, the osteocytes are located inthe lacunae The osteonic canals contain blood vessels that are parallel to the long axis ofthe bone These...
... agents include the naturally occurring vinca alkaloids (e.g vincristine and vinblastine) and their semi-synthetic analogues (e.g vinorelbine) and the taxanes (e.g paclitaxel and docetaxol) They ... on the microtubules, an essential part ofthe cytoskeleton of eukaryotic cells The vinca alkaloids prevent the protein from polymerizing into microtubules by binding specifically to β-tubulin In ... protein binding after IV administration (Extra et al., 1993) Docetaxel is mainly bound to alpha acid glycoprotein, lipoproteins, and albumin Among them, alpha acid glycoprotein is the main determinant...
... Services Department ofthe Weizmann Institute by a single injection of 150 lg ofthe purified protein, followed by two booster shots ofthe same amount of protein at 2-week intervals Serum was ... between the amounts of UP12 and the total amount of protein inthe extracts, a series of samples containing determined amounts ofthe purified UP12 were separated by SDS/PAGE along with the cell ... has been identified by other means as an invivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that aggregate at 42 °C in E coli containing a dnaK deletion mutation...
... that controls the protein expression inthe wild-type is the shuttling of Gal80p Increasing the amount of Gal80p inthe cytoplasm by increasing the distribution coefficient (K) causes the switch ... model provide insights into the importance of their roles inthe overall performance ofthe network A similar experimental evaluationofthe roles of individual elementary mechanisms invivo is very ... between the various components However, these in vitro experiments may not yield theinvivo mechanism ofthe regulatory system An experimental determination oftheinvivo mechanism in a genetic...
... Invivo tracking of 75 Se-labelled Der p intrinsic biological properties ofthe allergens may all contribute to their allergenicity [1–4] The intrinsic properties required for evoking an ... application of a newly developed technique for specific labelling of recombinant proteins by means of incorporating a radioactive selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the ... mimicking inhalation ofthe natural allergen The fact that i.t instillation of [75Se]Der p led to deposition of radioactivity in alveoli and bronchioli with no detectable allergen remaining in the...
... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G inthe transformed knock-out line does not reach the level ofthe ... conformation ofthe loop His-tags were employed in a topological study ofthe major light harvesting chlorophyll a ⁄ b binding apoprotein, Lhcb1 [18] Interestingly, fusion of a His-tag to the C-terminus, ... light harvesting complex (LHCI), consisting of four members (Lhca1–4) ofthe chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, inthe vicinity ofthe PSI-F and...
... previously to induce an increase inthe level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... reproduced inin vitro conditions if nNOS digestion by calpain is carried out inthe presence of HSP90 [27] This finding can also explain the large extent of digestion of eNOS in aorta, in which, in association ... milli-calpain isoforms were reduced to a limited extent in brain, whereas, in aorta (Fig 2B), the decrease inthe two protease isoforms was more pronounced Moreover, in brain, the natural inhibitor of...
... the survival of H-RS cells [2] Cytokines and chemokines operating in a complex interaction have been suggested to be involved inthe pathogenesis of HL [3] A number of studies indicate that the ... respectively Furthermore, the UV spectra ofthe materials in peaks II and IV were in agreement with the UV spectra for eoxins (Fig 4, inset) [11] To further analyze the identity ofthe materials in peak ... the orientation ofthe enzyme inthe membrane of L1236 cells and the exogenous addition of arachidonic acid reaching the active site ofthe cytosolic enzyme to a greater extent than that of the...
... http://www.translational-medicine.com/content/7/1/41 QK performing ex vivo experiments of vascular reactivity in WKY common carotid rings [12], and then we evaluated invivothe role of this small peptide studying the ... not bind to VEGFRs, indicating that the helical structure is necessary for the biological activity The N-terminus of these peptides is capped with an acetyl group, while the Cterminus ends in an ... hematoxylin and eosin [4] Quantitative analysis was done by counting the total number of endothelial cells, identified by lectin staining (see immunohistology), inthe Matrigel plug in each of 20 randomly...
... murine receptor resulted in a faint stain ofthe cytoplasm and rarely in staining ofthe membrane ofthe tumor cells (Figure 3B) Inthe more advanced 30 weeks tumors the staining patterns changed ... demonstrating the inverse correlation ofthe expression ofthe two tumor relevant molecules and implying their functional link Discussion The understanding ofthe mechanism responsible for theinvivo ... to the logarithm ofthe initial amount ofthe target DNA inthe sample The relative concentration of one target with respect to another is reflected inthe difference ofthe cycle number, the...
... in- vivoThe objective ofthe current study was to measure the change in apparent length ofthe ATFL and CFL, in- vivo, in uninjured healthy ankles during a dorsiflexion-plantarflexion and supination-pronation ... the understanding of function of these ligaments and injury mechanisms However, the in- vivo behavior ofthe lateral ankle ligaments during the range of motion has not been well studied Data in ... applying inversion/eversion and internal/external rotational forces during the flexion-extension arc of motion They demonstrated increased strain inthe ATFL with increasing plantarflexion, inversion...