in vivo and in vitro diagnostic tests of allergy
báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis for Engineering Design 1999, 34(1):1-15 ... motion In- vitro tests could at best simulate resorption by milling the bone interface at a predetermined location prior to testing [46] Nonetheless, the objective of in- vitro primary stability tests ... Measurement of the Relative Motion Between an Implant and Bone under Cyclic Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, whereas in compounds (a) and (d), the presence of N–H stretching and the absence of C=O ... peroxidation was induced by adding 10 µl of FeCl3 (400 mM) and 10 µl of L-ascorbic acid (200 mM) After incubation at 37oC for hr, the reaction was terminated by adding ml of 0.25 N HCl containing 150 ... presence of NaNH2 as a strong base in the reflux condition, and compound (f) was obtained by using acid chloride, i.e., acetyl chloride, at RT instead of using acetic anhydride in the presence of NaNH2,...
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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... with 0, 0.25, 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction of rooting In case of rooting experiment, 10 replicates per treatment were taken and the experiment was ... shoot having 3–4 nodes) within weeks of subculture (Tab II) (Fig 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... the indicated time-points For this, to 10 pellets per group and time point for each donor were pooled and homogenized using a pellet pestle and repeated titration in mL of Trizol reagent (Invitrogen,...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
... Olsen DB: Inhibition of HIV-1 reverse transcriptase by a quinazolinone and comparison with inhibition by pyridinones Differences in the rates of inhibitor binding and in synergistic inhibition ... fitting of the data using the single ligand binding equation y = (Bmax × [ligand])/(Kd + [ligand]) The DEC formation by TFV-terminated DNA/RT in the presence of dCTP yielded Bmax = 49.2 ± 2.7% and ... type infection in vitro by combination of delavirdine, zidovudine and didanosine Antiviral Res 1997, 34:51-63 Buckheit RW Jr, Watson K, Fliakas-Boltz V, Russell J, Loftus TL, Osterling MC, Turpin...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and ... JE, Santoro SA 1995;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis ... isolation and expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological...
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Genetic variation in growth, stem straightness, pilodyn and dynamic modulus of elasticity in secondgeneration progeny tests of Acacia mangium at three sites in Vietnam
... Queensland (QLD, Australia), Western Province, Papua New Guinea (PNG) and Indonesia (West Papua, adjacent to PNG and outlying populations in the Aru Islands and Ceram) Commonly used seed material in ... vector of fixed effect of genetic group, w is the vector of random row within replicate effect, n is the vector of random column within replicate effect, t is the vector of random effect of plot ... age and age 4, f is the vector of random family within genetic group effects, and e is the vector of random residuals XB ; XP ; ZW ; ZN ; ZT and ZF are incidence matrix relating m, p, w, n, t and...
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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of ... activity against strains of E coli, S aureus, S epidermidis, E faecalis and P aeruginosa, and strains of K pneumoniae and strains of K oxytoca, respectively The representative case of E coli E1...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... using a conditional packaging system [23] The proviral form of the vector is shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of ... 80% confluency, and transduced with increasing doses of IDUA vector (1, 10 and 50 ng of p24 viral protein) in the absence or in the presence of polybrene (5 lgỈmL)1) After a 24-h incubation at ... A cDNA into the brain of metachromatic leukodystrophy mice [21] and b-glucuronidase cDNA into the brain of mucopolysaccharidosis type VII mice [22], showing that the in vivo transfer of genes...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Specifically, we examined the role played by each polyamine in the self-assembly of in vitro ... rough indication of the degree of difference in complexity between the in vitro and in vivo nuclear settings For instance, it is easy to suppose that both the presence of many other ions in the ... DNA minor grooves [25] Even though in vitro and ‘natural’ NAPs share a series of structural characteristics, in the present article we are describing the in vitro assembly of polyamines and phosphates...
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Báo cáo khoa học: "Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes" docx
... in vitro in the absence of serum and somatic cells: amino acids, vitamins and culturing embryos in groups stimulate development Biol Reprod 1994, 50,390-400 Gordon, I and Lu, K H Production of ... follicular and metestrus phases However, Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes 69 Ta ble In vitro maturation, fertilization and cleavage rates of bovine ... levels in the circulation resulting in the regression of dominant follicle and emergence of constant follicle wave turnover [21] The reasons for poor in vitro developmental competence of oocytes...
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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf
... effect of Medicinal plant extracts on HCV infection, serum infected Huh-7 cells were again seeded after three days of infection in 24-well plates in the presence and absence of herbal extracts and ... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s protocol Total RNA was extracted by using Trizol...
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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps
... composed of lamins and NE lamin binding proteins including Nesprin1 alpha, MAN1, lamina-associated polypeptides-1 and (LAP1, LAP2), emerin and Lamin B receptor[254,255] The four latter and Lamin B ... we included interactions involving a minimum of two proteins Non-interacting and self-interacting proteins were excluded to simplify the network representation The resulting network consists of ... specific and composed of bands of distinct size and intensity, representing putative proteins interacting with Tat The purified recombinant proteins GST and GST-Tat are indicated by an arrowhead...
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Báo cáo y học: "Optic nerve sonography in the diagnostic evaluation of adult brain injury" pptx
... noninvasive and invasive ICP measurements in brain-injured patients mmHg in only 39% of the measurements They interpreted this error as because due either to the inherent inaccuracy of the noninvasive ... distribution of CSF in cases of brain trauma and midline shift, in which the ICP of the intraparenchymal probe may differ from that affecting the blood flow in the area of the middle cerebral artery In ... measurements of ICP in brain-injured adults Noninvasive evaluation of the ICP was performed using TCD and invasive measurements of the ICP were performed by means of a surgically placed intracranial...
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Báo cáo khoa học: "Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication" potx
... and the minimum single oral dose that induced vomiting in swine was determined to be 0.075 mg/kg bw [7] The feed contamination level of DON in Plasma diagnostic indicators of deoxynivalenol intoxication ... pro-inflammatory cytokines in rodent adipose tissue: studies in vivo and in vitro Horm Metab Res 2003, 35, 142-146 Creppy EE Update of survey, regulation and toxic effects of mycotoxins in Europe ... Scott PM Possibilities of reduction or elimination of mycotoxins present in cereal grains In: J Chelkowski (ed.) Cereal Grain: Mycotoxins, Fungi and Quality in Drying and Storage pp 529-572,...
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Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... role, such as in DNA replication, in living cells In contrast, mutants of AtRPA70b were viable but showed high sensitivity to UV and MMS, suggesting involvement of AtRPA70b in the repair of damaged ... region, and a third, DBD-C, in the C-terminal region DBD-A and DBD-B are the most important regions for binding of ssDNA, whereas DBD-C has only weak ssDNA-binding activity At the N-terminus of RPA70kDa...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro transcription and translation ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, ... (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), based on this ratio about 40% of GalNAcA in both CPS and O-chain polysaccharides was present in the form of a primary amine When...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... pneumoniae SPase I For in vitro self-cleavage of S pneumoniae SPase I in the presence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated in 37 °C for h in 20 mM Tris/HCl ... inactivates the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including ... X-100, 10% glycerol, and 50 lg of E coli lipid extract For selfcleavage in the absence of phospholipid, 20 lL of reaction containing lg of purified SPase I was incubated at 37 °C in the same buffer...
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not ... examined for their properties in enzyme-linked lectin and cell binding assays, histopathological monitoring, and organ biodistribution [37] The utility of our combined approach and the validity of...
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