in vitro in vivo release from injectable dispersed systems
Báo cáo y học: " Modulation of the virus-receptor interaction by mutations in the V5 loop of feline immunodeficiency virus (FIV) following in vivo escape from neutralising antibody" ppsx
... exposed in vivo to soluble CD134L (for example helper T cells in proximity to CD134L-expressing antigen presenting cells) In contrast, viruses interacting through CRD1 alone may be suppressed in vivo ... (SU and TM), amino acid numbering is indicated above in SU, and in TM (H648Q, T651A, V655M) (Fig 2) The specific targeting of the region adjacent to and including the predicted N-linked glycosylation ... 96) for 30 minutes at 37°C In assessing whether pre-incubation with Fc-CD134 enhanced CD134-independent infection, virus was pre-incubated with Fc-CD134 for 30 minutes prior to plating on the...
- 13
- 344
- 0
Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
... from interacting with MT2, we have shown that in vitro it suppresses the metal release from metallothioneins This points to an hitherto unknown mechanism or compound(s) being involved in this interaction ... interaction in living cells and information about this additional factor is needed in order to perform physiologically relevant future in vitro studies and in the interpretation of results obtained from ... of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of the b-domain in MT isoforms and...
- 9
- 398
- 0
Báo cáo khoa học: Choline increases serum insulin in rat when injected intraperitoneally and augments basal and stimulated aceylcholine release from the rat minced pancreas in vitro pptx
... on insulin release from minced pancreas To determine if choline elicits insulin release in vitro, minced pancreas was incubated with various concentrations of choline The rate of insulin release ... acetylcholine levels in the pancreas Effect of HC-3 on choline-induced increases in acetylcholine release from the minced pancreas To determine whether choline-induced increases in acetylcholine release are ... blocked the choline-induced increase in insulin (Table 1) Effects of relatively selective antagonists of muscarinic receptors on choline-induced increases in serum insulin To determine the involvement...
- 9
- 338
- 0
Báo cáo y học: "Cyclooxygenase inhibition lowers prostaglandin E2 release from articular cartilage and reduces apoptosis but not proteoglycan degradation following an impact load in vitro" ppt
... following traumatic injury The inhibition by COX inhibitors of PGE2 release following trauma may provide an opportunity for early clinical intervention to reduce cell death from apoptosis Our in vitro ... incorporation of proline into collagenase digestible proteins in human articular chondrocytes: identification of an effector autocrine loop involving insulin-like growth factor I Mol Cell Endocrinol 1996, ... hyaluronic acid in samples of OA cartilage in vitro Non-selective NSAIDs have differing effects on cartilage metabolism Some stimulate, some have no effect, and others – including indomethacin – inhibit...
- 10
- 241
- 0
Báo cáo y học: "In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures" pps
... obtained from Sigma Chemical (USA) Cyclophosphamide was obtained from Hengrui Pharmaceutical (China) Cell lines and cell culture Malignant cell lines in this study include human lung adenocarcinoma ... staining is shown in Figure The positive reaction located in cytosol was stained in brown The color of the stain is positively correlated to the protein expression Figure mouse tumor volume Inhibitory ... acetylshikonin The IOD of each group indicates that the expression of bax and caspase-3 in the acetylshikonin groups increased, whereas bcl-2 decreased in the same groups, resulting in 0.8 Adriamycin...
- 7
- 258
- 0
Nghiên cứu đánh giá đặc tính nông sinh học của một số dòng hoa đồng tiền (gerbera jamesonii) đột biến tạo được trong nuôi cấy in vitro và in vivo
... n nuụi c y in vitro 4.1.1 44 K t qu nghiờn c u kh nng trỡ c a cỏc d ng ch i cõy ng Ti n t bi n in vitro 4.1.2 44 K t qu nghiờn c u kh nng nhõn nhanh cỏc d ng ng Ti n t bi n in vitro 4.2 49 ... triphosphate BAP : 6-Benzylaminopurine CV : Correlation of Variants D : DMSO : Dimethyl sulphoxide EMS : Ethyl methane sulphonate G : G ch Ki : Kinetin (6-Furfurylaminopurine) LSD : Least Significant ... B ng Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng hoa mu tr ng) 49 B ng Kh nng sinh tr ng v nhõn nhanh c a cỏc d ng ch i ng Ti n t bi n in vitro (gi ng...
- 112
- 763
- 0
Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... were grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) ... subsequent incubation with the indicated retinoids was then terminated at the indicated time points by washing with NaCl ⁄ Pi and lysis of the cells using mL of Trizol (Invitrogen) per well Total RNA from...
- 17
- 406
- 0
Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx
... strong incorporation of [3H]Thd into growing daughter strands occurs, preferentially sedimenting in the first third of the gradient and attributable to short chains originating from newly initiated ... entering S-phase in succession Separating a cell fraction containing DNA bound proteins Entire replicative SV40 minichromosomes bearing functionally bound replication proteins can be eluted from ... chromatin cannot be eluted from isolated nuclei Interrupting the continuity of the DNA (e.g by suitable endonucleases) yields elutable chromatin fragments preferably originating from regions far from...
- 11
- 610
- 0
Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... mediate interaction with transcriptional activators in vitro [33] Recombinant activator interaction domains interact with activation domains in vitro using a two-step mechanism, where rapid ionic interaction ... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... activator-binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results suggest that the coupled binding...
- 9
- 539
- 0
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... checkpoint signaling in vivo It may function in the sensing of DNA damage [22] In contrast with the intensive study of RPA in animals and yeast, little is known about this protein in plants In 1997, ... transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein was purified ... weakly with DNA In RPA32-kDa, the DBD-D domain is located in the central region In yeast, RPA1 can only bind to the RPA2 ⁄ dimer, in contrast with our findings which show binding of OsRPA70b to...
- 12
- 588
- 0
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... to leave room for altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested ... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of PSI-G in vivo, samples of thylakoids from wild-type...
- 9
- 422
- 0
Báo cáo khoa học: Oxidative stress induces a reversible flux of cysteine from tissues to blood in vivo in the rat pptx
... ROS, as indicated by the lack of increase in lipid peroxidation observed in in vitro and in vivo experiments [21] In previous studies [22,23], we observed that a single intraperitoneal administration ... propargylglycine on the cysteine increase Increase in cysteine (sum of reduced cysteine + disulfides) over levels at time zero in rat blood induced by diamide infusion (7.73 lmolÆmin)1Ækg)1 intravenously) after ... observed in aging) is sufficient to cause a large increase in the oxidized forms of intracellular proteins bearing vicinal thiols, which can in uence specific signaling pathways [44] In addition,...
- 13
- 510
- 0
Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition, ... determined by the intensity ratio of fragments at m/z 662.2 Da (GalNAcAN-containing trisaccharide repeating unit) and m/z 663.2 Da (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset),...
- 10
- 332
- 0
Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes from ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... explain why self-cleavage is happening in vivo, one speculation is that it may be involved in regulating the activity of the enzyme It is not difficult to imagine that bacteria may secrete proteins...
- 9
- 351
- 0
Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot
... signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... occurrence of ricin resistance in CHO cell mutants being attributed to GnT-III activity [15,16] is an example in which an ensuing decrease in affinity in plant lectin binding can help to explain the new ... is known from the plant lectin erythroagglutinating phytohemagglutinin (E-PHA), whereas the presence of a bisecting GlcNAc in a core-fucosylated biantennary N-glycan did not affect binding to the...
- 17
- 481
- 0
Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... genes into adipose tissue in mice (see also patent no US5476926) In conclusion, our results indicate that respiratory uncoupling per se is capable of inducing mitochondrial biogenesis in vivo ... (1994) In vitro interactions between nuclear proteins and uncoupling protein gene Ó FEBS 2002 10 11 12 13 14 15 16 17 18 19 20 21 promoter reveal several putative transactivating factors including ... UCP1 in unilocular adipocytes in subcutaneous than in epididymal fat and suggest that UCP1 is not capable of inducing conversion of a unilocular into a multilocular adipocyte UCP1-induced increase...
- 10
- 555
- 0
Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
... differences in replication in mice reflect real differences in the biology of these viruses, or are related to differences in the animal models including route of infection In HSV, direct intracranial inoculation ... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... replication in vivo In HSV, deletion of the dUTPase homolog did not effect the replication of the virus in vitro, however its virulence was reduced by 1000-fold in mice following footpad inoculation...
- 6
- 330
- 0
Báo cáo hóa học: " N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo" ppt
... RNA from infected cells that were not treated with SCH 16 showed the presence of JEV proteins (Figure 4) In vivo evaluation of compounds against JEV using mouse model After ascertaining the in vivo ... depicting the in vivo effect of SCH 16 against a lethal JEV challenge Survival graphs depicting the in vivo effect of SCH 16 against a lethal JEV challenge SCH 16 was administered to swiss albino ... Screening for inhibition of virus induced cytopathic effect in vitro The antiviral activity assay of the Ribavarin and MIBT derivatives against JEV, Den-2 virus or WNV were screened in vitro using...
- 12
- 407
- 0
Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... a grant from the Bingtuan Doctor Foundation Program (05JC04, XinJiang, China) References survival 40% 11.1% survival Competing interests 5LD50 20LD50 FMD3 in this study, siRNAs targeting highly ... http://www.virologyj.com/content/5/1/86 ously injected in the neck with 50–100 ug of plasmids dissolved in 100 ul of saline Mice of the control group were subcutaneously injected with saline After h, the suckling mice were...
- 6
- 256
- 0
báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology 1998, 157:157-168 ... Figure activin-A and follistatinin vitro with control medium, FSH cell number and theper replicate)oestradiol,combination inhibin-Atreating granulosa cells (N = replicates with wells included per...
- 13
- 438
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25