in vitro cell free assays

... were sticking to the cell membrane, then lysing cells after incubation with MTS reagents followed by centrifugation (removing cellular debris) could eliminate the interference in reading from ... Dulbecco's minimum essential medium [DMEM]: Ham's F-12 media containing 10% heat inactivated foetal bovine serum [FBS], mM L-glutamine and 1% penicillin streptomycin [Penstrep] (all purchased from Invitrogen ... various assays in discriminating the subtle difference in the level of effect those nanoparticles could impose to cells Another consideration in assessing the sensitivity of the cell viability assays...

Ngày tải lên: 20/06/2014, 20:20

... 264.7 cells incubated with MNPPEI-PEG nanoparticles The cells were incubated at different concentrations as indicated over a 72-h incubation Cell viability was determined using common assays including ... various assays in discriminating the subtle difference in the level of effect those nanoparticles could impose to cells Another consideration in assessing the sensitivity of the cell viability assays ... reported in most studies were used in the present study We validated the cell viability data obtained from commonly used MTS, CellTiter-Blue and CellTiterGlo assays by comparing them with that obtained...

Ngày tải lên: 21/06/2014, 17:20

... PCL/PLGA solvent evaporation In vitro Indomethacin PCL melt-dispersion In vitro Nitrofurantoin Insulin PCL PCL solvent evaporation solvent evaporation In vitro In vitro Gadzinowski et al.34 Cao and ... PCL/2,2-bis(2oxazoline) Techniques Vitro/ Vivo Researchers In vitro In vitro Pitt et al.42; Buntner et al.43,44 Martini et al.45 Guerra et al.46 both Limin et al.47 In vitro Barbato et al.48 In vitro Cha ... al.21,22,23 Cyclosporine PCL solvent evaporation In vitro Aberturas et al.24 Cisplatin PCL/PLA solvent evaporation In vitro Chandy et al.25 E- and P-selectin Ribozymes PCL PCL In vitro In vitro Dickerson...

Ngày tải lên: 28/11/2015, 13:53

... processes occurring in vitro represent, in large part, what happens in virus-infected cells, there are also differences between virus production in vivo and in vitro In the in vitro system a large ... The viral proteins most directly involved in RNA replication include protein 3AB, the precursor of 3A, which is a small membrane binding and RNA binding protein, the terminal protein VPg, RNA polymerase ... of plus strands increases simply as a result of more minus strands remains to be determined The fact that the RNA binding domain of the protein in 3Cpro is involved in stimulating RNA synthesis...

Ngày tải lên: 19/06/2014, 08:20

... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding ... GSM-900 and cell stress in skin models S Sanchez et al expression under stress conditions has been reported in a number of cell types, including skin cells The major HSPs expressed in the skin [5] ... expression (Fig 6J) In the case of Hsp70 (Fig 6G–I), instead of being expressed only in the cytoplasm as in NHDFe, it was also expressed in the nucleus UVB exposure induced a slight increase in Hsp70 expression,...

Ngày tải lên: 07/03/2014, 11:20

... CDO/chemotherapy combinations in GEO cells resulted in significant reductions in cell number This was further investigated by assessing BrdU incorporation Results confirmed the synergistic reduction in cell number, ... increased in GEO but unchanged in HCT116 cells (B) Standard immunoblots for cyclin and CDK levels in GEO cells (C) Immunoprecipitation experiments highlighting cyclin/CDK interactions in the GEO cell ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy...

Ngày tải lên: 07/03/2014, 15:20

... enzymes cleaving the target protein Other effects which can occur in whole cells (e.g the induction of new proteins interfering with the protein expression) cannot occur in our isolated in vitro system ... Our in vitro data indicate that CSPs are directly involved in this downregulation of protein expression by binding to elements with intermediate specicity which occur in most genes According ... existing in vivo, the unspecic inhibition of protein expression observed in the living cell after a cold shock can be explained by a direct binding of CSP to RNA and or DNA Besides this, in our...

Ngày tải lên: 07/03/2014, 21:20

... apoptosis in testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction ... pathways involved in testicular germ cell apoptosis pertaining to specific treatments As previously shown [7], testicular cell apoptosis follows both extrinsic and intrinsic pathways The extrinsic ... resuspended in medium-199 containing 100 lgÆmL)1 streptomycin sulfate and 100 IUÆmL)1 penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess...

Ngày tải lên: 16/03/2014, 04:20

... future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based ... UK); Cellomics NF-jB Hit kit Evaluation Screening (Cellomics Inc., Pittsburg, PA, USA); DMEM (Gibco, Invitrogen); 200 mm l-glutamine; fetal bovine serum (Gibco Invitrogen, Virkon, Pzer internal ... lL; prewarmed in a water bath at 37 C) was dispensed into each well and incubated for 15 (prewarming xative is critical to maintaining cell integrity) After 15 min, the plates were inverted to...

Ngày tải lên: 16/03/2014, 11:20

... Ca2+-binding EF-hand family protein Plastid movement impaired (PMI2) Ca2+-binding EF-hand family protein Protein kinase Zinc-finger (C3HC4-type RING finger) family protein Zinc-finger (C3HC4-type RING ... Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis Plant Cell 19, 1898–1911 Podell S & Gribskov M (2004) Predicting N-terminal myristoylation sites in plant proteins ... To investigate the mode of protein N-myristoylation in plant cells in more detail, we established a cell- free protein N-myristoylation system as an application of an advanced wheat germ cell- free...

Ngày tải lên: 29/03/2014, 21:20

... remove cells remaining in the intra-trabecular spaces and plated at fragment per well in a 24-well plate containing ml of control medium (α-MEM containing 1% Gentamicin v/v and 10% foetal bovine ... increasing the action of the first and negating that of the second That drugs can interact is not unexpected Systematically investigating such interactions in vitro is slowly becoming a routine ... 43(2):133-52 Meletiadis J, Mouton JW, Meis JF, Verweij PE: In vitro drug interaction modeling of combinations of azoles with terbinafine against clinical Scedosporium prolificans isolates Antimicrob...

Ngày tải lên: 20/06/2014, 01:20

... monitoring of dynamic changes induced by cell- chemical interaction [38–41] Since the relevant test is labeling free, the RT-CES assay allows real-time, automatically and continually monitoring cellular ... respectively Initially, 104 cells were seeded into each well containing 200 lL cell culture mediums in 96-well plate and incubated for 24 h and then added the relevant materials and incubated at ... shown in Fig as well as in the supporting information, our studies indicate that when there was only daunorubicin (1.0 10-7 mol/L) in the cell system, the dynamic response of SMMC-7721 cancer cells...

Ngày tải lên: 21/06/2014, 18:20

... nisalahcotyc lm/gµ gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim ... dehsaw ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 ... gip fo egavaelc rewol yltnacifingis dewohs EM-β gniniatnoc muidem denifed a ni derutam setycoo taht gnidnif eht derorrim hcihw ,egavaelc oyrbme desaerced yltnacifingis muidem noitarutam eht ot...

Ngày tải lên: 07/08/2014, 20:23

... comparing the effect of hydration on assay response, a paired t-test was used = For in vitro assays, the experimental unit was as a family of pollen grains arising from a single clone including ... taining After 48 h, germination was scored based on the percent of grains in each of categories: Class 1, pollen grains elongated greater than twice the original hydrated diameter of the grain; ... (1974) for determining significant response differences at the 95% confidence level For pollen lots germinating in the 50% range, 300 grains were observed and for lots germinating either > 90%...

Ngày tải lên: 08/08/2014, 23:22

... this is the first report presenting the extensive in vitro cellular studies including radiation and chemical cell survival/ toxicity curves with the cell line originating from chordoma Acknowledgements ... bleocin, B) cisplatin, C) mitomycin C, and D) camptothecin Black square represents HeLa cells, white triangle indicates U87-MG cells, and black reverse triangle indicates U-CH1-N chordoma cell line ... of cell lines A) Growth curves for three cell lines Black reverse triangle indicates HeLa cells, white circle indicates U87-MG glioma cells, and black circle presents chordoma origin U-CH1-N cells...

Ngày tải lên: 09/08/2014, 09:21

... than HePC in preclinical investigations [29,51] Intriguingly, in our in vitro study ErPC3 was a more effective inducer of apoptosis in T98G cells than ErPC when given alone and in combination with ... and ErPC3 in human malignant glioma cell lines in vitro Both drugs sensitized human malignant glioma cell lines to radiation-induced cell death including apoptosis and enhanced radiation-induced ... tumor cells in human astrocytoma/glioblastoma cell lines in vitro Results ErPC induces time- and concentration-dependent apoptosis in human malignant glioma cell lines We have shown earlier that induction...

Ngày tải lên: 09/08/2014, 10:20

... II IVD cells were seeded into alginate beads at a low cell density of 106 cells per millilitre and a final alginate concentration of 1.2% The alginate beads were then incubated in combinations ... following Additional files are available online: Additional file 'Live/dead' staining of intervertebral disc (IVD) cells in alginate cultures A representative image is shown of IVD cells in alginate ... viability in alginate was assessed by 'live/dead' scoring, in which live cells fluoresce green and dead cells fluoresce red (Live/Dead; Invitrogen Ltd.) [25] Cell senescence Senescent cells express...

Ngày tải lên: 09/08/2014, 10:23

... maintained in RPMI 1640 medium (Invitrogen Corporation) supplemented with 10% FBS The J774 cell line (derived from murine macrophages, kindly donated by Robin May, University of Birmingham, UK) was maintained ... epithelial cell line, were also maintained in RPMI 1640 supplemented with 10% FBS L929, a mouse fibroblast cell line, was obtained from ECACC (number 85011425) and cells were grown in DMEM/F-12 ... [32] Disc cells were certainly much more effective at ingesting the beads than the fibroblast cell line, L929 Cells in other cartilaginous tissue are also reported to be capable of becoming phagocytic;...

Ngày tải lên: 09/08/2014, 10:23

... phosphate-buffered saline stimulation (Figure 2) Similar findings were obtained in human primary B cells/B -cell lines In contrast to resting B cells, when 'professional antigen-presenting cells' (for example, ... Similarly, ODNs INH-44, INH-46, and INH-48 are linear derivatives of INH-18 lacking CCT, GGG, or both triplets INH-4 and INH13 are palindromic or linear analogues of INH-1 and INH-18, with the ... bases, resulting in complete palindromes (class R) or in non-palindromic linear sequences (class B) Class R INH-ODNs were between 10- to 30-fold less potent as inhibitors in resting mouse B cells than...

Ngày tải lên: 09/08/2014, 14:21