in vitro and in vivo degradation of phytate

... al In vivo degradation of NOS and HSP90 by calpain B A C D Fig In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material (A) and ... brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, ... compilation ª 2008 FEBS 2503 In vivo degradation of NOS and HSP90 by calpain M Averna et al Table Levels of native and 15 kDa calpastatin species in brain and aorta of NMS and HMS rats treated with...

Ngày tải lên: 30/03/2014, 04:20

... analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments ... chromatography Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory ... were obtained by venipuncture and separated in aliquots One of them was collected in tubes containing EDTA and assigned to determine haematimetric indexes, plasmatic protein concentration and rheological...

Ngày tải lên: 10/08/2014, 05:21

... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro transcription and translation ... pools of plants expressing similar levels of PSI-G, and analysed by immunoblotting, using antibodies to PSI-G and PSI-F, as an indicator of the relative content of PSI in the samples Plants lacking...

Ngày tải lên: 07/03/2014, 21:20

... molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and ... assessed the effect of MSCs on in vitro and in vivo T-cell proliferation as well as on CIA By using MSCs of both IFN-gR KO and wild-type origin, we also addressed the role of IFN-g in the immunomodulatory ... responses in vitro [28] To strengthen the experimental background for future therapy with MSCs, we addressed the effect of MSCs on in vitro and in vivo Tcell proliferation and on CIA in this study In...

Ngày tải lên: 12/08/2014, 11:23

... having an impact on the in vitro behavior of both BoHV-5 strains Concerning the in vivo characterization, in spite of the in vitro differences discussed above, we observed that both BoHV-5 strains ... sensitivity of the ELISA test available In the case of animal 191, as discussed above, the reason could have been the lack of infection Page of Conclusions The in vitro and in vivo properties of two ... effects of sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating...

Ngày tải lên: 12/08/2014, 18:22

... study, and informed consent was obtained from the patients' next of kin Hygrometric measurements AH and relative humidity were measured during the inspiratory phase in the in vitro and in vivo ... 12 different settings Combinations of two tidal volumes (0.5 and l), two peak inspiratory flow (0.5 and l/s) and three levels of minute controlled ventilation (5, 10 and 15 l/min), with the ventilator ... Yearbook of Intensive Care and Emergency Medicine Heidelberg: Springer-Verlag; 2002 23 Martin C, Papazian L, Perrin G: Performance evaluation of three vaporizing humidifiers and two heat and moisture...

Ngày tải lên: 12/08/2014, 20:20

... Phosphoinositide 3-kinase Promyelocytic Leukemia Zinc Finger protein Phosphatase of Regenerating Liver Retinoblastoma Protein Red Fluorescence Protein RNA interference Receptor Tyrosine Kinase Standard ... down-regulated cyclins D and E by 16h and induced the expression of p21waf1/Cip progressively The increasing expression of cyclin E in MV4-11 cells at 4h, in MOLM-14 cells at 1h and cyclin D in MOLM-14 ... In vitro and In vivo study of ABT-869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors: insight into molecular mechanism and biologic...

Ngày tải lên: 11/09/2015, 09:06

... engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, maintenance and substitution of damaged tissues and organs using principles of ... modulators in both in vitro and in vivo environments The two specific aims of this research was To investigate the effects of bone regeneration, scaffold degradation and mechanical properties of PCL-TCP ... for signs of sustained delivery when immersed in PBS solution Heparin was chosen to improve binding and regulate release of BMP-2 here and concurrently act as a framework for binding of endogenous...

Ngày tải lên: 09/10/2015, 11:18

... IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI EXPRESSING HOUSE DUST MITE ALLERGEN LIEW LEE MEI 2009 IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI ... leukotrienes and basic proteins (cationic proteins, eosinophil peroxidase, major basic protein and eosinophil-derived neurotoxin), and they might be an important source of interleukin-3 (IL-3), ... of cytokines in splenic cultures Figure 4.5 Oral feeding of recombinant Lactobacillus rhamnosus GG 108 induced the Blo t 5-specific IgG2c production in mice Figure 4.6 Oral feeding of recombinant...

Ngày tải lên: 09/10/2015, 11:18

... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... CHAPTER 4: OPTIMIZATION OF NATIVE AND CUSTOMIZED SCAFFOLDS IN VITRO AND THEIR EFFECTS IN INITIAL BONE HEALING 4.1 INTRODUCTION 44 4.1.1 In vitro degradation study 44 4.1.2 In vivo degradation study ... and histology In the second part of the study, in vitro and in vivo degradation behaviours of these treated scaffolds were investigated PCL-TCP scaffolds were monitored after immersion in standard...

Ngày tải lên: 09/10/2015, 11:18

... (anti-microtubular) agents include the naturally occurring vinca alkaloids (e.g vincristine and vinblastine) and their semi-synthetic analogues (e.g vinorelbine) and the taxanes (e.g paclitaxel and docetaxol) ... discussed in Chapter In Chapter 5, in vitro cellular study of transferrin conjugated LPNPs is performed using MCF7 human breast adenocarcinoma cells In Chapter 6, in vivo pharmacokinetics, complete ... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude...

Ngày tải lên: 09/10/2015, 11:18

... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... causing the clearance of drugs once they are present in physiological system is the high probability of binding to endogenous proteins in the circulatory system The high-binding affinity of most...

Ngày tải lên: 09/10/2015, 11:18

... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... and reversibly to the β- subunit of tubulin in the microtubules rather than to tubulin dimers The binding site to tubulin differs from the one of vinca-alkaloids and podophyllotoxins While vincas...

Ngày tải lên: 09/10/2015, 11:18

... lung, and kidney were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... novel hydrophobized polysaccharide/oncoprotein complex vaccine induces in vitro and in vivo cellular and humoral immune responses against HER2-expressing murine sarcomas Cancer Res 58:3385–90 Gustafson,...

Ngày tải lên: 23/04/2013, 21:38

... stranded sequence was determined for a region of this construct containing galA and some of the flanking regions, resulting in the genomic sequence of A niger galA The presence of one putative intron ... and 0.3% L-arabinose and is predominantly linear Potato arabinogalactan consists of 86% D-galactose and 6.6% L-arabinose, while soy arabinogalactan consists of 57% D-galactose and 38% L-arabinose ... by introducing an NsiI site at the translation start point of galA and by then cloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking...

Ngày tải lên: 21/02/2014, 01:21

... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used ... growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose (m) or in...

Ngày tải lên: 22/02/2014, 07:20

... [27] In rats Fig Western blot analysis of p53 and c-Jun in liver and lung Total protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in ... liver and lung indicating that the amount of p53 protein was significantly increased in the liver and lung from vitamin A-deficient rats that in controls c-Jun protein was significantly decreased in ... cared and handled in conformence with the Guiding Principles for Research Involving Animals and Humans, approved by the Council of the American Physiological Soceity The School of Medicine Research...

Ngày tải lên: 08/03/2014, 08:20

... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, in vivo imaging of miRNA regulation ... vector containing three tandem repeat regions of miRNA23 binding sequence (reprinted with permission [11]) information for understanding the miRNA biogenesis related to human diseases Imaging miRNA...

Ngày tải lên: 16/03/2014, 01:20

... substrates of Synoviolin were found to include polyglutamine-expanded huntingtin [18], the tumor suppressor Synoviolin is involved in the degradation of nicastrin gene p53 [19] and Parkin-associated ... Synoviolin increases the membrane localization of huntingtin protein [18], also suggesting that Synoviolin is involved in intracellular trafficking We also found that NCT interacts with Synoviolin ... Synoviolin is involved in the degradation of nicastrin T Maeda et al NCT is one of the essential cofactors of the c-secretase complex We therefore investigated the effect of the Synoviolin-mediated degradation...

Ngày tải lên: 23/03/2014, 04:20

... height of 175 cm and average body mass index (BMI) of 21.8 kg/m2) were included in this study None of the participating subjects had subjective complaints of ankle pain, previous history of ankle injury ... inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion Ozeki et al.[7] investigated strain changes in central fibers of the lateral ankle ligaments in ... numerous in- vitro studies which investigated the changes in ATFL and CFL strain in response to loading in various positions Colville et al.[10] measured strain in lateral ankle ligaments in 10 cadaveric...

Ngày tải lên: 20/06/2014, 01:20