in situ rock stress measurement

... adding and delet- ing amino acids. Positive symbols indicate an increasing amount, and negative symbol indicate a decreasing amount. No. amino acids added or subtracted Change in length of linker ... struc- tural proteins such as collagen-19 (COL-19), nonery- throcyte spectrin, a-actinin and filamin A within living cells, and that the FRET from this cassette changes with stress in situ. The efciency ... proteins, inclu- ding COL-19 (Fig. 10G), nonerythrocyte spectrin (Fig. 10E), filamin A (Fig. 10C), and a -actinin (Fig. 10A), and expression systems including HEK-293, 3T3 and C. elegans, and the insertion...

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 09/08/2014, 04:20

Ngày tải lên: 05/11/2014, 22:17

... colonization of large intestines of streptomycin-treated mice. Infect. Immun. 67, 6152–6156. [37] Felske, A., 1998. In situ detection of an uncultured predominant Bacillus in dutch grassland ... trong vi sinh vật học là fluorescein-derivates (Fluorescein- Isothiocyanate (FITC), 5(6)carboxyfluorescein-N-hydroxysuccimide-ester (FluoX)) và rhodamine-derivates (Tetramethyl-Rhodamine-Isothiocyanate ... in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. Infect. Immun. 62, 673–679. [40] Forsgren, J., 1996. Persistence of nontypeable Haemophilus influenzae...

Ngày tải lên: 30/10/2012, 11:02

... sử dụng avidin (hay streptavidin) – biotin: đây là một cặp ligand có hằng số ái lực cao nhất tìm thấy trong sinh học. Đầu tiên, mẫu dò đƣợc đánh dấu bằng biotin theo hai cách: biotin đƣợc gắn ... với dung dịch Blocking buffer 1X, chất blocking trong dung dịch sẽ khóa các phân tử lai không đặc hiệu lại.  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase ... DỤC VÀ ĐÀO TẠO ĐẠI HỌC NÔNG LÂM TP. HỒ CHÍ MINH BỘ MÔN CÔNG NGHỆ SINH HỌC  KHOÁ LUẬN TỐT NGHIỆP ỨNG DỤNG PHƢƠNG PHÁP IN SITU HYBRIDIZATION ĐỂ CHẨN ĐOÁN MẦM BỆNH WSSV...

Ngày tải lên: 05/11/2012, 14:01

... the combined study, we will illustrate the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria on fine scale in autotrophic nitrifying biofilms by applying the full-cycle ... allows relating in situ microbial activity directly to occurrence of nitrifying bacteria population. KEYWORDS : Nitrifying biofilms, 16S rDNA-cloning analysis, fluorescent in situ hybridization ... performed. In situ hybridization of vertical biofilm thin sections clearly indicated that the numbers of probe NEU-stained NH 4 + -oxidizing bacteria (i.e., Nitrosomonas marina-lineage, Nitrosomonas...

Ngày tải lên: 05/09/2013, 08:40

... substrates for the enzymes involved in metabolism of acetylenic compounds, resulting in products that are suitable for bacterial growth. We isolated a Pseudomonas putida strain growing on 2-butynedioate ... found in more natural products than bromides, chlorides, or nitriles [1]. Com- pounds containing triple bonds are widely distributed in plants, fungi, bacteria, and other living organisms [1]. In addition ... putida strain growing on 2-butyne- dioate or propynoate as sole carbon source. To investigate the acetylene bond biodegradation, we used in situ proton nuclear magnetic resonance ( 1 H-NMR) in water...

Ngày tải lên: 08/03/2014, 08:20

... Zhang). 1 Also working in College of Chemistry, Jilin University, Changchun 130021, PR China. a multitude of applications, such as in gas sensitivity, sensors, and inductors [11–13]. In recent years, ... been increas- ing interest in the synthesis of conductive organic/inorganic nanocomposites. A few of works had been reported on the synthesis PANI/inorganic nanocomposites [14–16]. Hopkins et ... College of Science, Beijing Institute of Technology, Beijing 100081, PR China Received 21 September 2007; accepted 5 January 2008 Available online 10 January 2008 Abstract Polyaniline/magnetite nanocomposites...

Ngày tải lên: 20/03/2014, 13:04

... Hodgkin T., 2000. A training guide for in situ conservation on farm. Version 1. Rome, International Plant Genetic Resources Institute. 7. Quy T. D and Hue N.T.N (editors), 2006. In situ Conservation ... is maintained and sustained operation. In this context, the appreciation of the actual status, indicate the limitations and reasons, which propose the approaches to promote conservation insitu ... yet promoted insitu conservation of crop genetic resources in our country. In the paper, some policy, administrative and technical approaches are also proposed. Keywords: In situ conservation,...

Ngày tải lên: 23/03/2014, 17:21

... believed to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). Such concerted reactions result in formation of the cornified ... detecting in situ activ- ity of TGase 3 in a frozen mouse skin section. As shown in Fig. 6, in the presence of CaCl 2 , specific incorporation of FITC–pepE51 (1 lm) in endogenous glutamine-acceptor ... consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes [23,24]. Further- more, TGase 3 in hair follicles is involved in cross- linking...

Ngày tải lên: 29/03/2014, 21:20

... tin-bismuth alloy nanoparticles as nanosensors with a sensing mechanism of size increase induced by melting. Tin-bismuth was chosen as a candidate for investigation because its melting point ... schemes in order to find a configuration that maximizes collection efficiency. Finally, reservoir engineering techniques will be tailored to promising nanosensors in order to estimate measurement ... a field study in which a conservative tracer (fluorescein) and a reactive tracer (rhodamine WT) were injected into the Steamboat Hills reservoir. The decay kinetics of rhodamine WT were extrapolated...

Ngày tải lên: 30/03/2014, 20:20

... ground ã Mining companies ã Mining Processing ã Crushing ã Milling ã Concentration ã Mining companies (at mine head) ã Processors ã Mining (if in- house at mine head) ã Manufacturing (if ... capital investment in mining and mining MFP. ã Accounting for the lag between capital investment and output in mining explains a considerable amount of the year to year variability in mining MFP. ... IN THE MINING INDUSTRY Figure 4.1 Total cost shares in mining, by industry, 2004-05 0 20 40 60 80 Coal Oil and gas Iron ore Non-ferrous metal ores Other mining Services to mining Mining All industries Intermediate...

Ngày tải lên: 01/04/2014, 00:20

... aspirate buffer. 3. Incubate while rocking for 1 h at room temperature in the dark in 100 µL of staining buffer containing 10 µg/mL of FITC-conjugated streptavidin. Wash by diluting the sample with ... colabeling of DNA damage and specific cellular proteins. 2. Development of In Situ DNA Labeling 2.1. Characterization of DNA Polymerase Activities and the Invention of In Situ DNA Labeling In ... with 5 àg/mL proteinase K in PBS for 30 min and wash in PB for 30 min. 4. Postfix the treated specimens with 0.25% GL in PB for 30 min and wash in PB for 10 min. 5. Immerse them in distilled water...

Ngày tải lên: 11/04/2014, 09:47

... chromosomes is described in Subheading 3.9.4.). Carry out all dipping and washing steps in Coplin jars or slide tanks. 1. Denature the DNA in situ by dipping the slides in 0.07 M NaOH (made from ... wash solution C 3ì for 3 min each. 6. Stain with DAPI by immersing the slides in 0.2 àg/mL of DAPI in 2ì SSC for 5 10 min at room temperature. Rinse in 2ì SSC 3ì for 2 min each. 7. Mount the slides ... the slides in 2ì SSC solution for 2 min. 3. Dehydrate the slides by incubating for 3 min in 75%, 90%, and 100% ethanol and air dry. Human Partial Chromosome Paints 7 9. Vortex. 10. Coplin jar. 11....

Ngày tải lên: 11/04/2014, 09:47

... after rinsing in distilled water. 6. In the case of labeling with biotin-16-dUTP or fluorescein-12-dUTP, a fourfold decrease in the concentration of dTTP in the PRINS reaction mix resulted in ... for 2 x 5 min in washing buffer. Alternatively, you may wash the slides for 5 min in PBS and dehydrate them. 13. Mount the slides in Vectashield containing 0.5 pg/mL DAPI. 14. Examine the slides ... Air-dry Embed in a protein matrixC Time Temperature 30 min 37°C 5 min 37OC 10 min 30 mm 30 min Room temperature 37Y! 37°C 5-10 min 37OC 1-2 min 37°C 1s Room temperature 10 min Room temperature...

Ngày tải lên: 11/04/2014, 10:11

Ngày tải lên: 22/04/2014, 16:14

Ngày tải lên: 06/05/2014, 08:55