in situ functional imaging of sensory receptors in lung models

... under the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene ... function of increasing amount of total proteins in membrane fractions from I7 yeast strain induced at 15 °C, and from yeast expressing SSTR2 as a control activity By taking advantage of structural ... demonstrated that mRNA of the olfactory receptors is present in both uninduced and induced yeast cells (Fig 1) This indicates a leakage of GAL1 ⁄ 10 promoter in glucose-containing minimal medium The...

Ngày tải lên: 07/03/2014, 16:20

... protein, they were incubated with cells expressing this glycoprotein complex Fig 10 Binding of soluble gp 120 or cells expressing gp120/gp41 to proteoliposomes containing CD4 and CCR5 A: Binding of ... expression vector (Invitrogen, Groningen, the Netherlands) in order to obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus A plasmid containing the wild ... concentration; insertion of detergent into the membrane should result in a gradual decrease of RET [41,42], and lysis of the liposomes in abrupt abolition of RET A decrease in RET results in an increase...

Ngày tải lên: 08/03/2014, 09:20

... to fragmented adhesion region in the cell center Vinculin staining on the whole cell surface in most of the cells (green, G); some cells still have vinculin staining similar to starved cells (arrow) ... increase in Akt phosphorylation (Fig 6D) Within 30 of the addition of the agonist, phosphorylation had declined; in the case of p-ERK1 ⁄ 2, concentrations fell to basal, whereas in the case of ... phosphorylation was inhibited by  70%, returning to the level observed after the addition of fresh medium in the absence of agonist (Fig 6C, inset) Incubation in media containing specific receptor...

Ngày tải lên: 30/03/2014, 08:20

... with increased ability to complete the physical performance tests and in carrying out ADL (Table 6) Informants were present in 1167 (97%) of the interviews Comparison of the physical health rating ... Years of life expectancy free of problems with PADL 12.32 13.43 7.81 8.33 Years of LE free of problems with all ADL % of total LE free of problems with PADL 93.3 91.2 % of total LE free of problems ... for paying attention to programmes aimed at preventing and postponing disability and dependency Such emphasis is essential for improving the quality of life of the elderly, even in developing countries...

Ngày tải lên: 14/02/2014, 06:20

... domain (LBD) of ecdysone receptors Fig S2 Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors This supplementary material can be found in the ... et al Interspecies comparison of ecdysteroid receptors Fig EMSA using CHO cell extracts following transfection and incubation in the absence and presence of MurA, RH5849, and RH5992, using the ... ligand-binding pocket, thus blocking potentiation mediated by USP binding to JHIII Finally, although MET explains some JH-mediated activities in T castaneum, it does not account for all of them...

Ngày tải lên: 18/02/2014, 08:20

... the MCPIP gene The coding sequence lacking the PIN domain was obtained in two steps First, the fragment of 423 bp situated in front of the PIN domain was amplified by PCR using forward primer 5¢CGGGATCCCGGAGTCTGAGCTATGAGTG-3¢ ... is that MCPIP regulates the amount of IL-1b mRNA Involvement of PIN domain of MCPIP in the stability of IL-1b mRNA In order to find out whether the PIN domain in MCPIP is responsible for IL-1b ... shown) Of the identified proteins, 73% are proteins involved in mRNA stability, but there are also proteins involved in other cellular processes, such as actin Further studies characterizing proteins...

Ngày tải lên: 18/02/2014, 14:20

... addition, the N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal region of BMAL1 in a mammalian two-hybrid assay Accordingly, a Ó FEBS 2004 Interaction of DEC1 with ... results indicate that the bHLH region, including His57 and Arg65, is responsible for the E-box binding Determination of the region in BMAL1 for binding to DEC1 To identify the region in BMAL1 ... activity of DEC1 Determination of the binding domain of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previously demonstrated by a yeast two-hybrid assay [7], and this interaction may be involved...

Ngày tải lên: 19/02/2014, 16:20

... (1988) Lysine ⁄ fibrin binding sites of kringles modeled after the structure of kringle of prothrombin Proteins 3, 85–96 20 Thorsen S (1975) Differences in the binding to fibrin of native plasminogen ... formation in the absence of 6-Ahx Data were fitted as indicated in Fig (IC50 ¼ mM) (B) Kinetics of plasmin formation performed on cells (main figure) and fibrin (inset) in the presence of varying concentrations ... Ponting CP (1998) Evidence that the conformation of unliganded human plasminogen is maintained via an intramolecular interaction between the lysine-binding site of kringle and the N-terminal...

Ngày tải lên: 20/02/2014, 01:20

... lacking leucine in the presence of thiamine for inhibition of the nmt promoter For visualization of Ydc2–GFP constructs, yeast cultures were grown in selective media in the absence of thiamine ... [14,20] Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs [19], which is reminiscent of the ... positioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein The truncated Ydc2 mutant proteins localize to the mitochondria in S pombe Fig Binding...

Ngày tải lên: 20/02/2014, 11:20

... rates in the presence of kirromycin The effect of kirromycin on the growth of GRd.tsf was investigated by measuring growth rates of cultures in LB containing various concentrations of kirromycin ... translation indirectly by inhibition of mRNA synthesis during amino acid starvation [20–23] Kirromycin is one in a family of antibiotics that inhibits protein synthesis by binding EF-Tu–GTP and preventing ... hydrolysis of the GTP at the ribosome [25] Kirromycin has been shown to stimulate the nucleotide exchange in EF-Tu by increasing the dissociation of GDP and binding of GTP [26] In addition, the binding...

Ngày tải lên: 21/02/2014, 00:20

... H pylori strains harbouring a functional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro -in ammatory production of cytokines, mainly interleukin (IL)-8 [12] ... pneumoniae choline-binding protein A When H pylori bacteria were investigated in the absence of host cell contact, Cagd was found to enrich in the membrane fractions, even if a minor contribution ... turns of helices E and F, and the C-terminus helices I and J In addition, there is a lysine-rich N- and C-terminus, in accordance with the basic isoelectric point of CagS However, these lysine...

Ngày tải lên: 06/03/2014, 00:21

... obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig 2) PCR reactions performed in ... domain Taking into account the important role of Ptas, the results obtained in the present work, dissecting the different domains forming E coli Pta, will help in the future manipulation of these...

Ngày tải lên: 06/03/2014, 11:20

... expressing mutant GALE was increased to reach a detectable level of protein Tubulin serves as a loading control The relative amount of mutant protein was calculated based on the ratio of the band intensities ... galactose was determined by measuring BrdU incorporation The level of BrdU incorporation into each cell line cultured in the absence of galactose was set to 100% and the corresponding results from ... accordingly Relative protein abundance (RPA) was calculated based on the ratio of intensity of the myc–GALE to tubulin bands in the western blot and normalized by setting the amount of GALE in ldlD(myc–GALE)...

Ngày tải lên: 07/03/2014, 00:20

... temperature The secretin-like PilQ was detected in sufficient amounts in the inner and outer membranes The presence of PilQ in inner membranes is interesting, because secretin-like proteins of type IV pili ... implicated in retraction of the PilA4-comprising DNA translocator transporting the DNA through the periplasmic space Binding of the DNA to the DNA-binding protein ComEA on the surface of the inner ... acylation and, thereby, be involved in membrane anchoring of the pilin Since PilA4 is similar to the meningococcal pilin and contains several central serine residues, it is tempting to speculate that...

Ngày tải lên: 07/03/2014, 12:20

... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of ... (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell ... Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila receptor tyrosine kinase...

Ngày tải lên: 07/03/2014, 21:20

... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 26 Wang, W & Malcolm, B.A (1999) Two-stage PCR protocol allowing introduction of ... at some point in the reaction Modest changes in Km,ATP are seen in H44Y and R68C That H44 in uences the binding of both substrates suggests that the binding sites are probably close in space R68...

Ngày tải lên: 08/03/2014, 02:20

... ¯ow rate of mLámin)1 The following gradients were used: incubations containing arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held ... Naringenin-7-GlcRha (naringin) Hesperetin-7-GlcRha (hesperidin) Phloretin-7-Glc (phlorizin) Oleuropein Cyanidin-3-Glc (kuromanin) Cyanidin-3,5-diGlc Flavanones Dihydrochalcones Secoiridoids Anthocyanidins Ó FEBS ... glycosides, 17% A/83% B initial (1 min), increasing B to 90% (10 min), held at 90% (4 min) The column was re-equilibrated (5 min) in the appropriate starting solvent conditions following gradient development...

Ngày tải lên: 08/03/2014, 16:20

... involved in the binding of UDPGal and that E264 and W315 are involved in the GlcNAcbpNP binding site The residue E264, which is part of the conserved EDV motif, is involved in both substrate binding ... ferase toxin [32], using photoaf®nity labeling, the DXD motif has been implicated in the binding of UDP-Glc and Mn2+ It has been proposed that the DXD motif is involved in the folding of a small ... found in b1,3-GalTs and b1,3-N-acetylglucosaminyltransferases, thereby suggesting that they are more likely involved in mediating the b1,3-linkage speci®city rather than in the direct binding of...

Ngày tải lên: 08/03/2014, 16:20

... %Sj) of (–)-a-pinene The sabinene side product of Cl(–)bPINS was determined to be 74% e.e of (–)-sabinene (Table 2) ClcTS produced (–)-a-pinene as a side product with an e.e of 24%, but (+)-b-pinene ... in the present paper Only the origin of the trace amounts of linalool, a-terpineol and (E)-b-ocimene that are also present in the lemon extract remain unexplained, as they are not a product of ... after drying dissolved in mM Tris, pH 6.8, 0.2% (w/v) SDS and 1% glycerol Protein concentration was determined using the BCA Protein assay kit using BSA as protein standard reference, according to...

Ngày tải lên: 08/03/2014, 23:20

... protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the active site in the N2 proteins was independent of the nature of the C-terminal domain, in contrast ... protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the other chimeric protein, bearing the N-terminal domain ... the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–triglyceride interface The inactivation of N2Cc in the absence of bile salts was prevented...

Ngày tải lên: 16/03/2014, 06:20