in addition to its role

... triphosphate to the 5¢-hydroxyl group of thymidine, forming dTMP. Human TK1 is cytosolic and its activity is absent in resting cells, appears in late G 1 , increases in S phase coinciding with the increase ... myco- plasmic origin [14], the feedback inhibitor dTTP was bound in the substrate pocket, similar to the binding of dTTP to the D. melanogaster multi-substrate deoxyribonucleoside kinase [16], despite ... maintaining a proper balance between the dNTPs. In addition to the ribonucleotide reductase-controlled pathway, the dTTP level is con- trolled by thymidine kinases and TMP nucleotidases, forming...

Ngày tải lên: 18/02/2014, 12:20

... dford, MA, USA) and the radioactivity retained on the ®lter was measured. Staining with lectin and antibody Lectin staining and indirect immuno¯uorescence staining were carried out as described previously ... sugars. To identify the transport substrate of DmNST, a DNA fragment containing the entire c oding region was ampli®ed by PCR and inserted into the expression vector pMKIT-neo utilizing the EcoRI ... econdary antibodies in indirect immuno¯uorescence and Western blot analysis, respectively. Site-directed mutagenesis and insertion of an hemagglutinin tag We utilized the megaprimer method [17] to obtain a mino- acid...

Ngày tải lên: 21/02/2014, 15:20

Ngày tải lên: 18/12/2013, 21:45

... in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 domain (amino acids 456–620), we analyzed five EGFP-fused KIND2 domain ... EGFP- KIND2-1 to -KIND2-5 (Fig. 4A), by a pull-down assay with GST-CD2 (MAP2 v-KIND binding core). Input (cerebellum) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND Input (MAP2) GST v-KIND KIND1 v-KIND ... stain) EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 KIND2-1 487 KIND2-2 521 KIND2-3 555 KIND2-5 589 KIND2-4 488 456 620 KIND2 v-KIND + + + – – Interaction EGFP EGFP- KIND2-1 Input IB:...

Ngày tải lên: 14/02/2014, 19:20

... In Chang cells, stained occludin was observed in the cytoplasmic region. Additionally, in Occ WT -overexpressing Hep3B and Huh7 cells, regions stained with antibody to hem- agglutinin (HA), indicating ... findings, our research provides new insights into the role of exon 9 in the regulation of apoptosis and invasion in liver cell lines. A B Fig. 7. Effects of occludin shRNA on apoptosis and invasiveness. ... [23,24]. In contrast, in the case of occludin, an ER-mediated increase in intra- cellular calcium levels is not considered to be the mechanism underlying its effects in cancer cell apopto- sis and invasion...

Ngày tải lên: 18/02/2014, 18:20

... copper binding or loss of its binding could be related to specific cerebellar function(s) of stefin B [18], which remains to be seen by more in vivo studies. Stefin B as a copper binding protein We ... places, but in particular, stefin B has a number of histidines in the C-terminus (box in Fig. 1) at sites: 92, 75, 66 and 58. As histidine residues are central to copper binding in many proteins they ... found to bind to stefin B at pH 7 but not to stefin A (Fig. 2). The bind- ing isotherm data were fitted to sequential binding site parameters and the best fit, producing the smallest v 2 values, indicated...

Ngày tải lên: 19/02/2014, 06:20

... is associated with mutations in the AUH gene. This indi- cated that AUH, in addition to its RNA binding func- tion, must play an important role in leucine catabolism. AUH was overproduced in E. coli and characterized by ... had another function in cellular metabolism. Indeed, AUH had enoyl-CoA hydratase activity, which was described as an additional intrinsic function of the protein. Its role in intermediary metabolism, ... present work was initiated to kinetically charac- terize AUH on its presumed natural substrate 3-MG- CoA using a new strategy for its synthesis and developing a new assay. In addition, a mutant...

Ngày tải lên: 19/02/2014, 07:20

... by determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into cellular proteins. The Caco-2 cell line was ... status in cells and the fourth group in glutamine metabolism. Annexin A2 and A4 belong to a family of soluble cytoplasmic proteins that can bind to the membrane surface in response to elevations in ... labelling of 20 distinct proteins, which are indicated in bold. NQ, No glutamine-containing peptides in spectrum peaks. C-term, C-terminal part of protein; N-term, N-terminal part of protein. Spot Accession number...

Ngày tải lên: 20/02/2014, 01:20

... was transformed into E. coli strain TOP10F¢ (Invitrogen). The transformed cellsweregrowntoaD 600 at 37 °CinSOBmedium supplemented with 50 lgÆmL )1 ampicillin. To induce pro- tein expression, isopropyl ... removed and minced with a surgical knife. The minced tissue was wrapped in cotton gauze and suspended in NaCl/P i containing 100 lgÆmL )1 penicillin/ streptomycin at 37 °C for 3 h. After incubation ... cDNAs were determined by the Sanger dideoxy chain termination method using a PRISM TM Ready Dye Terminator Cycle Sequencing Kit (PerkinElmer). DNA samples were ana- lyzed using an automated sequencer...

Ngày tải lên: 20/02/2014, 11:20

... CLAP with a phosphoprotein stain did not reveal phosphate additions to these proteins. Only lanes in the gel containing the known phosphoproteins ovalbumin, b-casein and pepsin gave a reaction. Thus, ... doi:10.1111/j.1432-1033.2004.04338.x binding domains, so the presence of a GTP/ATP binding region in CLAP may indicate an energy dependent mech- anism for pro-CLAP processing or cathepsin L docking and stabilization not found in ... fasciclin domains in CLAP with other fasciclin I containing proteins is clear (Fig. 9). Using BLAST (http://www.ncbi.nlm.nih.gov/BLAST) to identify related proteins, a putative cell adhesion protein from...

Ngày tải lên: 07/03/2014, 16:20

... ankyrin repeat motif-containing proteins play an important role in protein–protein interactions. ANKHD1 proteins are known to possess multiple ankyrin repeat domains and a single KH domain with ... proteins also participate in protein–protein interactions via their repeat motifs [5,6]. Using yeast two-hybrid system analysis, we identified a protein containing a single ankyrin repeat that interacts ... may play a role in promoting proliferation and preventing apoptosis [8]. dMASK mediates receptor tyrosine kinase (RTK) sign- aling, independent of MAP kinase (MAPK) by either functioning downstream...

Ngày tải lên: 07/03/2014, 21:20

... containing arbutin or salicin as substrate; 100% C initial, i ncreasing D t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; 5% B/95% A initial (5 min), increasing ... the enzyme/inhibitor mixture to 180 lLof substrate (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP. Protein assays and protein sequencing Total protein in crude and ... (rutin) Quercetin-4¢-GlA Kaempferol-3-Glc Isorhamnetin-3-Glc Flavanones Naringenin-7-GlcRha (naringin) Hesperetin-7-GlcRha (hesperidin) Dihydrochalcones Phloretin-7-Glc (phlorizin) Secoiridoids Oleuropein Anthocyanidins Cyanidin-3-Glc (kuromanin) Cyanidin-3,5-diGlc Fig....

Ngày tải lên: 08/03/2014, 16:20

... commission’s findings, the Committee on the Assessment of the Role of Solar and Space Physics in NASA’s Space Exploration Initiative chose to interpret its charge in the broadest sense and to examine ... difficult to incorporate into the long planning cycles needed to get a mission into the strategic mission queues. The Explorer program can also provide opportunities to respond rapidly to specific ... this wind, traveling through interplanetary space, impacting planetary magnetospheres, and finally penetrating beyond our solar system. It is these fast particles that pose a threat to exploring...

Ngày tải lên: 14/03/2014, 10:20

... splicing of the intron RNA into the insertion site in the DNA are shown at the top. Solid lines indicate DNA and dashed lines correspond to intron RNA. M. D. Molina-Sa ´ nchez et al. Group II intron ... RNA-binding domain (domain X) associated with RNA splicing or maturase activity and a C-terminal DNA binding ⁄ DNA endonuclease region. The intron-encoded protein encoded by the mobile group II intron ... involving a RNP complex containing both the intron RNA and the IEP [7–9]. The group II IEPs have an N-terminal RT domain homologous to retroviral RTs, followed by a putative RNA-binding domain...

Ngày tải lên: 15/03/2014, 09:20

... and economy's financial sector. They analyze the streamlining public investment. But the in- role that interest rate policy could play in creases in interest rates should be high enough to Egypt's ... deposits in the economy's total money supply. Indeed, as depicted in Figure 2, the ratio of foreign currency deposits held in domestic banks to the total stock of money, i.e. M, has increased ... liberalized investment environment and in a financial system dominated by the banking sector, interest rates will be the key financial prices to guide investment decisions and to ensure...

Ngày tải lên: 15/03/2014, 14:20

... domains are related to its zinc-binding prop- erties. In vertebrates, zinc-binding proteins in serum, such as albumin and vitellogenin (which circulate in the bloodstream and transport zinc between ... that CFMYP diminishes its zinc- binding capacity by losing at least one zinc-binding site after its role in zinc transport is completed. We believe that all of the CFMYP incorporated into the oocytes is ... have zinc-binding motifs containing the catalytic or structural zinc commonly found in zinc enzymes and gene regulatory proteins. Such well- known zinc-binding motifs are also not found in the sequence...

Ngày tải lên: 16/03/2014, 05:20

Ngày tải lên: 23/03/2014, 17:21

Ngày tải lên: 24/03/2014, 00:21

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 03/04/2014, 09:20