iii reactions catalyzed by solids

... intrinsic kinetic deuterium isotope effects for Fig Oxidation reactions with coumarins catalyzed by P450s 2224 7-OEt coumarin O-deethylation by some rat P450 enzymes (today known as P450s 2B1 and 1A1) ... should apply to in vivo considerations The conclusions may apply to other P450 reactions, at least to those catalyzed by P450 1A2 Experimental procedures Chemicals 7-OMe and 7-OEt coumarin were ... isotope effects on cytochrome P-450 -catalyzed oxidation reactions: the oxidative O-dealkylation of 7-ethoxycoumarin J Biol Chem 259, 3000–3004 11 Gillette JR, Darbyshire JF & Sugiyama K (1994) Theory...

Ngày tải lên: 16/03/2014, 13:20

... this is induced by MMOB, although not by MMOBtru Earlier studies by small-angle X-ray scattering spectroscopy (SAXS) have also shown that such a conformational change is induced by the active MMOB ... cyanoaldehyde) was readily observed by gas chromatography that was confirmed by mass spectrometry No product was observed with MMOH alone or when MMOBtru was added instead of MMOB The reactions inferred to ... be that an inhibitory product was formed by MMOH but that it was readily removed in the flow-cell experiment As dioxygen is a substrate in sMMO -catalyzed reactions, it was possible that the product...

Ngày tải lên: 17/03/2014, 09:20

... under these conditions, the synthesis of poly(A) catalyzed by yeast poly(A) polymerase around sixfold Ó FEBS 2002 Poly(A) polymerase activation by dinucleotides (Eur J Biochem 269) 5325 Fig Effect ... After incubation at 37 °C, aliquots were taken and analyzed by TLC Lane (– E): control without enzyme Fig ATP consumption catalyzed by yeast poly(A) polymerase The reaction mixtures (0.035 mL) ... filter, but were retained by the precolumn or column The enzyme activity could be followed either, by measuring the decrease of the ATP content in the reaction mixture or by treating the reaction...

Ngày tải lên: 21/02/2014, 01:21

... 2GSH ! Ascorbate þ GSSG Thus, the reduction of DHA by DHAR is a ter-bi reaction We measured the initial velocity of the reaction catalyzed by DHAR with the concentration of GSH fixed at 0.2, 0.3, ... DHAR -catalyzed reaction does not include a constant, and show that the DHAR -catalyzed reaction proceeds via a ping-pong mechanism Two mechanisms have been proposed for ping-pong-ter-bi reactions: ... enhancement of the rate by limitation of photosynthesis Plant Cell Physiol 41, 335–343 29 King, E.L & Altman, C (1956) A schematic method of deriving the rate laws for enzyme -catalyzed reactions J Phys...

Ngày tải lên: 08/03/2014, 08:20

... 10(E),12(E)-tetradecadienoate by initial H-abstraction at C10 and 11(E)-tetradecenoate to 9(Z),11(E)-tetradecadienoate by initial oxidative attack at C9 [39] Whether these two transformations are catalyzed by separate ... attack catalyzed by a soluble plant D9 desaturase [37] However our results clearly demonstrate that calendate production is in fact initiated at C11 as might be expected for a process which is catalyzed ... activation by metaloxo species: oxidation of cyclohexane by chromyl chloride J Am Chem Soc 116, 1855–1868 24 Buist, P.H & Marecak, D.M (1992) Stereochemical analysis of sulfoxides obtained by diverted...

Ngày tải lên: 08/03/2014, 09:20

... by miniof 20 lgÆmL)1 each of phenylmethylsulfonyl fluoride and preparation followed by SacII digestion and its authenticity DNAse I, the cells were broken by a passage through a was confirmed by ... either determined by filtrating the samples through nitrocellulose filters and subsequent scintillation counting of the label retained by the filters as described previously [12] or by separating the ... the transcarbamoylation reaction catalyzed by HypF The formation of the HypE-thiocyanate involves first the carbamoylation of the C-terminal cysteine of HypE by interaction with HypF, then the...

Ngày tải lên: 16/03/2014, 18:20

... to 500 nm RESULTS Novel thermokinetic models for enzyme -catalyzed reactions For a simple single-substrate, single-intermediate, enzymecatalyzed reaction occurring in a batch reactor (BR) with ... calculated by the fundamental equations of thermodynamics [28]: Db G0 ¼ ÀRT Á ln KB m ð19Þ Db S ¼ ðDb Hm À Db G0 Þ=T m m ð20Þ Thermodynamics and kinetics of the cleavage of DNA catalyzed by BLM-A5 ... of DNA catalyzed by BLM-A5 at excessive ferrous ion and oxygen concentrations can be expressed as t0 ¼ k2 ½BLMŠ0 ½DNAŠ0 Km þ ½DNAŠ0 ð21Þ Here t0 is the initial rate for the DNA cleavage by BLM-A5...

Ngày tải lên: 24/03/2014, 04:21

... [estimated by the absorbance at 260 nm (A260)] The reaction was stopped by the addition of EDTA to a final concentration of mM and then cooled on ice The S1 fraction was obtained as the supernatant by ... the fractions were analyzed, by electrophoresis, on a 2% agarose gel, followed by staining with ethidium bromide The proteins precipitated from each 2.2-mL fraction, by adding 2.8 mL of 10% (w/w) ... B methyl ester [19] (kindly provided by Dr K Ogita, Sankyo Co., Tokyo, Japan) Incubations were carried out at 20 °C for h and the reactions were quenched by the addition of EDTA to give a final...

Ngày tải lên: 31/03/2014, 07:20

... buffer (pH 6.0) in a final volume of 1.0 ml After the reactions were terminated by boiling for 10 min, the products formed were quantified by high-performance liquid chromatography (HPLC) using ... indicated by vertical arrows The elution pattern was monitored by measuring at 240 nm (dotted line) The fractions containing the activator of FAD activity (filled circle) were examined for color reactions ... An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii Hui-Kai Huang1, Li-Fan Chen2, Masamichi...

Ngày tải lên: 20/06/2014, 21:20

... buffer (pH 6.0) in a final volume of 1.0 ml After the reactions were terminated by boiling for 10 min, the products formed were quantified by high-performance liquid chromatography (HPLC) using ... indicated by vertical arrows The elution pattern was monitored by measuring at 240 nm (dotted line) The fractions containing the activator of FAD activity (filled circle) were examined for color reactions ... An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii Hui-Kai Huang1, Li-Fan Chen2, Masamichi...

Ngày tải lên: 21/06/2014, 17:20

... aldol reactions catalyzed by primary amino acids and their derivatives 33 Table 2-2 The aldol reactions catalyzed by L-tryptophan in different solvents 36 Table 2-3 The aldol reactions catalyzed by ... 1.3.1.3 Intermolecular Aldol Reactions 1.3.2 Direct Asymmetric Aldol Reactions Catalyzed by L-Proline Derivatives 10 12 1.4 Direct Asymmetric Aldol Reactions Catalyzed by Primary Amino Acids and ... Asymmetric Aldol Reactions Catalyzed by L-Proline and its Derivatives 1.3.1 Direct Asymmetric Aldol Reactions Catalyzed by L-Proline 1.3.1.1 Introduction 1.3.1.2 Intramolecular Aldol Reactions - ii...

Ngày tải lên: 14/09/2015, 08:39

... Journal of Water and Environment Technology, Vol.1, No.2, 2003 micro-bubble by screw pump in the inner tube The size of micro-bubble is estimated to be less than 40×10-6 m ... upflow arrangements of flow third tank Figure Schematic diagram for improvement of water quality by removal of SS and biological filtration in closed water bodies a a: screw motor Ｍ b: biological ... is 3.4 m, minimum is 1.4 m, and average is 2.3 m Water depth changes around 0.4 m (up and down) by various reasons This plant was floated and fixed at the center of the raft Lake water was - 234...

Ngày tải lên: 05/09/2013, 08:40

... and pore formation by the T3SS P.-J Matteı et al ¨ des Sciences du Vivant (DSV), CEA P.J.M was supported by a PhD fellowship from the Rhone-Alpes ˆ region and T.I was supported by a PhD fellowship ... type III effectors into host cells in real time Nat Methods 2, 959–965 Mills E, Baruch K, Charpentier X, Kobi S & Rosenshine I (2008) Real-time analysis of effector translocation by the type III ... formation by type III toxin translocators of Pseudomonas aeruginosa Biochemistry 45, 8117–8123 50 Broms JE, Sundin C, Francis MS & Forsberg A ¨ (2003) Comparative analysis of type III effector...

Ngày tải lên: 14/02/2014, 22:20

... with purified recombinant calpain 7MIT, which was obtained by removal of GST and His6-tag by digestion with PreScission protease followed by ion exchange chromatography Purified calpain 7MIT was ... beads Pulldown products were visualized by staining with CBB Calpain 7MIT was pulled down by GST–IST1 and GST–MIM (Fig 5, Pulldown, open triangle) but not by GST–MIML326D,L353A or GST Enhancement ... they were probably derived by limited digestion with other cellular proteases, and not by autolysis of mGFP–calpain Enhancement of autolysis of mGFP– calpain 7–Strep by GST–MIM in vitro As we...

Ngày tải lên: 18/02/2014, 04:20

... retrocession business sold by the company, whereby the company is taking on the risk from the business Ceded – Related to reinsurance or retrocession policies bought by the company, whereby the company is ... period Losses arising from the unexpired portion of the policy are not covered by the loss reserve but instead are covered by the Unearned Premium Reserve It is possible that either reserve may become ... that have already occurred, the desired earned premium effect can be produced by adjusting unearned premium down by $75 Given that earned premium equals written premium plus beginning unearned...

Ngày tải lên: 06/03/2014, 15:21

... colloidal gold by low molecular mass biomolecules and by protein side chains Thus, in the present paper, we have tried to detail the reactions of a series of emerging antitumour gold (III) complexes ... residues by gold (III) complexes constitutes the molecular basis for their biological effects Concluding remarks In this study we have investigated the reactions of six representative gold (III) complexes ... [Au(bipy)(OH)2][PF6] and [Au(bipyc-H)(OH)][PF6] Further information on the reactions of gold (III) complexes with BSA was gained by the application of classical biochemical separation techniques The...

Ngày tải lên: 07/03/2014, 21:20

... introduced by Wagner et al to growth single crystalline silicon [9] A typical VLS growth starts with the dissolution of gaseous reactants into a nano-size metal liquid droplet, followed by nucleation ... Sn nanoparticles are pushed away from the substrate by continuous Si source feeding in the liquid–solid interface and it is lifted upward by the growing SiNWs As a result, large quantities of ... results denote that the as-synthesized SiNWs by our experimental method are pure crystalline silicon without oxygen and other impurities Conclusion Tin -catalyzed silicon nanowires (SiNWs) were synthesized...

Ngày tải lên: 16/03/2014, 15:10

... study, we investigate the morphological evolution of Si-NWs on Si substrates grown by nanoscale Au–Si island -catalyzed rapid thermal chemical vapor deposition (RTCVD) The initial nucleation of ... overpressure [4] Note that the growth rate of the NWs, R, is determined by the chemical potential difference at the interface of liquid/ solid by the relationship, R expðmSi =kTÞ [4] Correspondingly, the ... morphological and dimensional evolution of the Si-NWs on Si (0 1) and (1 1) surfaces grown 157 by using Au–Si nanoisland -catalyzed RTCVD For a given growth condition, a critical thickness of the Au film exists...

Ngày tải lên: 16/03/2014, 15:15

... was prepared by incubation of the protein solution (7.0 lM) with a small excess (two mol equivalents) of hydrogen peroxide for The transformation to the iron (III) species was followed by monitoring ... concentration range Formation of phenol dimers by the HRP/H2O2/NO2 system The phenol dimers formed by the HRP/H2O2/NO2 system during the nitration of and were analyzed by HPLC in the same conditions as reported ... thought to be responsible for phenol nitrations catalyzed by LPO, myeloperoxidase and HRP [12,16] So far, peroxynitrite has been excluded as nitrating agent by the analysis of 15N CIDNP experiments...

Ngày tải lên: 16/03/2014, 16:20

... procedure developed by Jorns et al [36], the enzyme activity was measured by monitoring the repair of cyclobutane pyrimidine dimers by DNA photolyase as a function of time by using UV–vis spectroscopy ... photorepair was performed by illuminating the mixture with 365-nm light from a dual wavelength UV lamp, and the repair of the cyclobutane pyrimidine dimers by DNA photolyase was followed by changes of the ... Acknowledgements This work was supported by the Deutsche Forschungsgemeinschaft (SFB-533, TP A5 and SFB-498, TP A2), by the Fonds der Chemischen Industrie and by the Hans-Fischer-Gesellschaft We thank...

Ngày tải lên: 16/03/2014, 18:20