identification of the apc gene

... predicted genes on flanking genomic regions of CArG boxes (A) Sequence positions of regions in the scaffolds from the WGS of tomato are indicated above the ends of the horizontal lines Positions of the ... to these CArG boxes Genes in the flanking regions of the RIN-binding CArG boxes and their expression To detect potential target genes of RIN, we analyzed the 5-kb genomic regions flanking the ... for the latter region (Figure 5A, Additional File 2) Gene predictions revealed that the CArGbox of 073F was located at the promoter region of the gene for SGN-U593726 [718 bp upstream of the...

Ngày tải lên: 11/08/2014, 11:21

... non-normality, for the existence of a major gene Assessing the relative importance of the sources of information is useful so as to judge the robustness of the model including F data The effects on ... present, a major gene was found in 100% of the cases For smaller increases of the variance (10%) major genes were still detected, and the probability of detection increased with the size of the data ... known The power for detection of a major gene is related to the unexplained variance in the model of analysis The inclusion of fixed and polygenic effects will therefore make the major gene easier...

Ngày tải lên: 14/08/2014, 20:20

... new cluster of ideas What matters is not the number of examples, the number of paragraphs, or the form your argument takes but, rather, the cogency of your ideas about the issue and the clarity ... well-developed analysis of the complexities of the issue by discussing the need for both the generalist and the specialist The argument is rooted in two extended examples, both well chosen The first (paragraph ... warn others, presumably for the most part motorists, of the presence of the roller skater It works only if the "other" is a responsible and caring individual who will afford the skater the necessary...

Ngày tải lên: 17/01/2014, 05:20

... In the present study, we report data on homologous expression of the nrdF gene of C ammoniagenes strain ATCC 6872 This is the first report of the successful purification of high amounts of the ... that of other class RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E We consider the first option unlikely Under these circumstances, the ... manganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study Rather, the involvement of manganese in the in vivo...

Ngày tải lên: 15/02/2014, 01:20

... Characterization of the promoter of the mouse Dnmt1 gene (A) Transcriptional activity of the mouse Dnmt1 promoter in NIH3T3 cells The top diagram shows the mouse Dnmt1 gene and the lower diagram shows the ... clarify the distinct roles of Sp1 and Sp3 at different phases of the cell cycle The transcriptional cofactor p300 is coprecipitated in complexes with Sp1 [38] The activation of the promoter of the gene ... through the same GA motif Sp1 and Sp3 bound to the same cis-element in the promoter of the gene for Dnmt1 Therefore, we next examined whether activation by Sp1 or by Sp3 affect expression of the gene...

Ngày tải lên: 22/02/2014, 07:20

... and function of the hormonal system We first cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate specificity than the aldosterone ... screening of the cDNA library did not result in the identification of any cDNA other than CYP11B1 (data not shown) Thus, we devised another strategy for the identification of additional genes of the ... To answer the question how these genes might have evolved we determined the complete genomic structure of both genes As shown in Fig 6, both genes exhibit the typical organization of the family,...

Ngày tải lên: 22/02/2014, 07:20

... Division, Office of the Attorney General David M Lyon, Principal Counsel, Office of the Attorney General, Department of Assessments and Taxation Craig A Nielsen, Principal Counsel, Office of the Attorney ...  c The name of the foreign LLC, LLP, or LLLP, and if different, the name it proposes to use in Maryland to business The address of the office that it is required to have under the laws of the ... interpretations of laws often vary Therefore, no guarantee can be given as to the accuracy and completeness of any information provided in this booklet The Attorney General’s Office and the Department of...

Ngày tải lên: 06/03/2014, 21:20

... mechanism of volatile general anesthetic action Results Binding of the volatile anesthetic halothane to the hydrophobic core of porcine odorant binding protein The binding of halothane to the porcine ... added to the reaction cell The lower panel depicts the binding isotherm of the calorimetric titration shown in the upper panel The continuous line represents the least-squares fit of the data ... This software provides the best-fit values for the heat of binding (DH°), the stoichiometry of binding (n), and the association constant (Ka) from plots of the heat evolved per mol of anesthetic...

Ngày tải lên: 07/03/2014, 16:20

... oversight of activities to ensure compliance with strategic priorities set by the Director General The Office of the Director General is composed of: (a) the Office of the Chief of Staff; (b) the Office ... Coordination Unit; and (i) the Occupational Health Unit A Office of the Chief of Staff 29 The Office of the Chief of Staff is the operational cell within the Office of the Director General and acts as ... support for the daily functions of the Director General and the Deputy Director General in the fulfilment of the Organization’s mandate The Office of the Chief of Staff facilitates the development...

Ngày tải lên: 08/03/2014, 16:20

... requested the Director-General of the ILO to bring this issue to the attention of member States and advise the Secretary-General of the International Maritime Organization of the action taken The Governing ... the Governing Body with the advice needed to guide the work of the Organization in this sector The maritime work of the ILO has had a profound effect on the industry The ratification record of ... intolerable denial of the freedom and dignity of their victims and undermine protection of the life and health of seafarers 137 The persistence of such threats to the lives of seafarers and the associated...

Ngày tải lên: 08/03/2014, 17:20

... in the health effects of physical activity, the report was planned collaboratively with representatives from the Office of the Surgeon General, the Office of Public Health and Science (Office of ... the Chemeheuvi of California, the Inca of Peru, the Zuni and other Pueblo peoples of the American Southwest, and the Iroquois of the American East, who also developed the precursor of modern-day ... The uses of exercise, I think, are twofold, one for the evacuation of the excrements, the other for the production of good condition of the firm parts of the body For since vigorous motion is...

Ngày tải lên: 14/03/2014, 20:20

... Of ce of the Auditor General Business Plan 2013–2016 The Office The Office of the Auditor General serves the Legislative Assembly and the people of Alberta Our mandate ... productivity and cost Office of the Auditor General Business Plan 2013–2016 Office of the Auditor General Budget 2013–2014 OFFICE OF THE AUDITOR GENERAL OF ALBERTA BUDGET BY OBJECT (thousands of dollars) ... behavior—these underpim the success of any organization • safety and welfare of all Albertans—especially the most vulnerable in our society • security and use of the province’s resources—they belong...

Ngày tải lên: 15/03/2014, 20:20

... and exert their effects on the expression of GnRH genes Estrogen The discovery of the estrogen response element ()441 to )428 bp) in the hGnRH-I gene [74] and the presence of both forms of nuclear ... majority of the studies have been done on the GnRH-I promoter, and there are only a few regarding regulation of the GnRH-II gene Regulation of GnRH gene expression The GnRH-I promoter In view of the ... is expressed mostly in the brain, the expression level of GnRH-II is much higher in other organs Analysis of the promoters of the GnRH-I and GnRH-II genes showed that they contain essentially...

Ngày tải lên: 16/03/2014, 04:20

... proved that iron control of the Regulation of the desferrioxamine gene cluster expression of the des cluster is mediated by the DmdR1 regulator Results Deletion of the desA gene of S coelicolor blocks ... boxes) in the upstream region of the diphtheria toxin (tox) gene of C diphtheriae and the desA gene of S pilosus Several putative iron boxes were found by bioinformatics analysis upstream of 10 different ... includes the entire siderophore biosynthetic gene cluster (Fig 2C) The failure of the 4204-bp fragment containing a wild-type copy of the desA gene to complement the mutation in trans suggests that the...

Ngày tải lên: 16/03/2014, 11:20

... downstream of pyrG were used to generate PCR products covering the entire pyrG gene downstream of synthetic promoters Expression from pyrG in the wild type is regulated by the concentration of CTP in the ... [24] This theory predicts that for biosynthetic pathways, such as those leading to the biosynthesis of amino acids or nucleotides, the control of the ux should reside in the demand for the end-product, ... expression of up to 250% of the wild-type level (Fig 4A) The results show that the feedback inhibition of the CTP synthase enzyme is incomplete in vivo In conclusion, the homeostasis of the CTP...

Ngày tải lên: 16/03/2014, 16:20

... procedure of Gilmore and Yamamoto [21] The effect of the PsbH absence on the structure of the PSII core complex was further probed by trypsinization of the D1 protein in isolated membranes of wild-type ... that the PsbH protein of photosystem II is a functional homologue of the H subunit from the reaction centre of photosynthetic bacteria This is in line with the effect of the protein on the herbicide ... difference either between the species or between the methods of the subcore preparation The instability of the IC7 subcore during electrophoresis of the isolated PSII core can be relevant to the situation...

Ngày tải lên: 17/03/2014, 11:20

... response of the MT gene to heavy metals [10,12], the presence of MREs in the promoter of the WD gene suggests that MREs and their cognate binding proteins function in the regulation of WD gene expression ... the ends of the Ku-80 gene The EcoRI site was used to generate the IR, and the BamHI site was used to ligate the Ku-80 IR gene to pcDNA3.1(+) to yield pc80IR Expression of the Ku-80 IR gene was ... MREs within the promoter region of the WD gene are indicated in Fig The four MREs are located in the proximal region of the WD gene promoter between )434 and +114, with MREa and MREe in the forward...

Ngày tải lên: 17/03/2014, 23:20

... ER retention (Fig 4A) The PGEL tetrapeptide is accessible at the surface of the pFGE molecule The PGEL C-terminus of pFGE is located on the surface of the molecule as part of an eight amino acid ... during pulse labeling After h of chase, only 25% of truncated pFGE were retained within the cells (Fig 4B) On the contrary, very little of the wild-type and the KDEL 1122 The canonical KDEL signal ... retention of human pFGE as a representative of the most common PGEL-containing pFGEs found in 21 different species Notably, the first two positions of the PGEL not match with any of the deposited...

Ngày tải lên: 23/03/2014, 07:20

... IB confirmed the identity of the T emersonii cel7 gene/ gene product; the location of these peptides in the deduced polypeptide sequence is given in the legend to Fig Comparison of the deduced ... hold the b-sandwich in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops ... which runs the length of the concave sheet, into which the cellulose substrate can be accommodated The b-sandwich represents the characteristic fold of GH7 and is also the fold of the legume-lectin...

Ngày tải lên: 23/03/2014, 13:20