... set of single mutants in each of the two positions, or of double mutants, was generated (Table 1) While Km values for all complexes not deviate from that of wild-type by more than a factor of ... bacterial enzyme by stripping subunits III and IV off the native complex, and by further destroying a large part of the acidic lobe(s) of the docking site of subunit II in the TM1 mutant Nevertheless, ... PhD Thesis, University of Frankfurt, Germany Turba, A., Jetzek, M & Ludwig, B (1995) Purification of the cytochrome c552 of Paracoccus denitrificans and sequence analysis of the gene Eur J Biochem...
... series of uorescence titrations of mant-ATP to CopB-B in the presence of various competitor concentrations [L0], the binding constant of the nucleotide can be determined from the slope of app ... of IR difference spectra of nucleotides in the presence and absence of CopB-B leads to the conclusion that the phosphate groups of ATP apparently not contribute signicantly to the formation of ... approximation of both domains with k1 the slow adoption of a closed conformation (species 2) The snapping of domains would initiate a rapid succession of catalytic steps (species and in Fig 7), of which...
... reciprocals of the concentrations of GSH (B) Reciprocals of initial rates of reduction of DHA were plotted against the reciprocals of concentrations of GSH at fixed concentrations of DHA The concentrations ... Double-reciprocal plots of the initial velocity vs the concentration of one substrate at various fixed concentrations of the other substrate (A) Reciprocals of initial rates of reduction of DHA are plotted ... of the reciprocals of the concentrations of GSH and, moreover, that both 1/f1([DHA]) and f2([DHA])/ f1([DHA]) are represented by the linear function of the reciprocals of the concentrations of...
... Reproducibility of the Experiment Results 33 3.2.2 Determination of Initial Reaction Rate 35 3.2.3 Determination of Order of the Reaction 39 3.2.4 The Effect of Temperature ... similarity of their structure and reactions of their cation radicals to those of the intensely studied diphenylanthracene and thianthrene radicals Examination of the kinetics and mechanisms of reactions ... of lipid deterioration, which leads to the development of rancidity, off-flavor compounds, polymerization, reversion, and other reactions causing reduction of shelf life and nutritive value of...
... matching The authors compared the costs and benefits of PROFO and concluded that the PROFO yielded internal rates of return of about 21% 27 Overview of the Study ANNEX Classification Study citation ... participation in PROFO in the early 1990s They compared a treatment group of 102 SMEs that had completed a three-year cycle of participation in PROFO by 1995 to a control group of 148 firms of similar ... productivity export share of sales 5% 13 to 17 % 24 % export share of sales export share of sales All other outcomes variables 40 % 50 % No impact Log(profits), log(sales), log(profits/L), log(sales/L)...
... of the ISAs It is assumed that practitioners already have a knowledge of the 2007 edition of IFAC’s Handbook of International Auditing, Assurance, and Ethics Pronouncements • Use of sound professional ... 145 Professional Skepticism .145 TABLE OF CONTENTS 2.8.4 Identification of Fraud Risk Factors 146 Examples of Fraud Risk Factors 147 2.8.5 Assessment of Fraud ... Identification of Risks 134 2.7.4 Assessment of Risks 136 Likelihood of Risk Occurrence 136 Monetary Impact of Risk Occurrence 136 2.7.5 Documentation of Risks...
... interpretation of this result is that the rate of substrate cleavage is not equivalent to the rate of chemistry, and that the rate of chemistry (extrapolated to be kc = 5.7ặmin)1) is faster than the rate of ... terms of the rate of reaction This would be benecial not so much in terms of the rate of the overall reaction, as the cleavage reaction is not the limiting step (binding is slower), but in terms of ... constant of the 5Â exon mimic, h is the fraction of 5Â exon mimic bound to the ribozyme, and [ribozyme]u is the concentration of unbound ribozyme in the reaction Measurement of rate constant of substrate-cleavage...
... a-(1fi4)-linked glucose units of pullulan (Fig 4A) IPU is an anomer-inverting enzyme Comparison of the active sites of the model of IPU and Dex49A The active site structures of the model of IPU (Fig 4A) and ... composed of residues 25–540 The overall structure of the model of IPU and the mutated residues in this study are shown in Fig To elucidate the mechanism of the substrate recognition of IPU, two ... Since the extremely low concentration of isomaltose is effective for the induction of amylase synthesis, the low activity of IPU may be suitable for control of this regulation Acknowledgements...
... coffee or not for the coffeemaker), or its level on a hedonic attribute (rating by consumers on comfort and ease of use for the mouse and rating by consumers on taste of the coffee for the coffeemaker) ... affirming effects of something can diminish its impact We find it unlikely that consumers are aware of the self-affirming benefits of choice of high design Therefore, discovery of this new form of affirmation ... Across all six conditions, 25 of 275 (9%) of respondents did not make both of the choices encouraged by the set-up involving price differences Of those 25 individuals, most of them (66%) were respondents...
... mechanism of CDH Results Redox properties of WT and F166Y CDH The redox potentials of WT and F166Y were compared at various pH values, as shown in Fig 2A The potential of F166Y was lower than that of ... pH dependence of the midpoint potential of heme in WT (h) and F166Y (d) (B) Absorption spectra of oxidized (solid line) and reduced (dotted line) forms of F166Y Midpoint potentials of heme in CDH ... reduction of flavin, and the absorption at the isosbestic point of heme (449.0 nm) decreased similarly in both Fig Stereo representation of the heme in ˚ CDH and the amino acids within A of the heme...
... analysis of the titration of H181A PETN reductase with progesterone (C) Plot of absorbance change as a function of 2,4 dinitrophenol concentration for the data shown in (A) (D) Plot of absorbance ... His-His pair, there has been no report of a systematic analysis of the contribution of each histidine residue to binding and catalysis Thus, to ascertain the role of each histidine residue, and to ... reductase Fig Analysis of ligand binding by equilibrium titration studies (A) UV-visible analysis of the titration of H181A PETN reductase with 2,4 dinitrophenol Inset, detail of the absorbance change...
... measure of the stability of the bound dioxygen In this way, if the values of kobs are plotted against the pH of the solution, we can obtain a stability prole of HbO2 or MbO2 in terms of the autoxidation ... fairly long period of diapause in safety In light of such unique behavior of the 4th-instar larva of T akamusi, it was of particular interest to investigate the molecular properties of the hemoglobin ... set of isosbestic points at 396, 426, 576 and 640 nm, indicative of no production of any intermediate species between the two forms To observe the time courses of the transformation reaction of...
... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... (Fig 5) Moreover, one of the carboxylate oxygens of Asp153 and the carboxamide oxygen of Asn153 are positioned in such a way as to allow metal coordination interaction with one of the manganese ions ... revealed by the conservation of the whole topology of the active site, would explain the essentially unaltered interaction of the D153N variant with the metallic cofactor In the modelled structure,...
... GTP activation of the glutaminase reaction and CTP synthesis was compared (Fig 5) In the absence of GTP, the rate of glutamine hydrolysis was signicantly higher than the rate of CTP synthesis so ... CTP synthesis assay (A) GTP activation (squares) of CTP synthesis For comparison GTP activation of glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of ... GTP activation of the glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of UTP and ATP-cS (C) GTP activation of CTP synthesis at 0.1 mM each of UTP and ATP...
... available for comparison of the performance of the individual tests, an analysis of results was done using a variety of standards Efficiency of microscopy, culture and PCR in terms of sensitivity, specificity, ... survey in a slum Quality of samples submitted METHODOLOGY Samples were processed using either Petroffs’s method or N-acetyl-L-cysteine-sodium hydroxide method 15 A small amount of the processed pellet ... tuberculous lesion @ 0% positivity of PCR is the expectation in this group Indian Journal of Tuberculosis 72 COMPARISON OF PCR WITH ROUTINE DIAGNOSTICS b PCR data of smear negative samples from tuberculosis...
... Institute of Scientific and Technical Information of the Academy of Sciences of the USSR (VINITI) and the German Zentrale Informationsverarbeitung Chemie, Berlin (ZIC) and supplied by the German software ... DE AB Process of oxidizing o-nitrotoluene in the side chain, by means of manganese dioxide and sulphuric acid, to o-nitrobenzaldehyde, as well as to carbon dioxide with excess of o-nitrotoluene, ... Technical Information of the Academy of Sciences of the USSR (VINITI) and the German Zentrale Informationsverarbeitung Chemie, Berlin (ZIC) and supplied by the German software company, InfoChem...