hybridoma technology for the generation of monoclonal antibodies

... C noxius venom To the best of our knowledge, this is the first report of the generation of a human recombinant antibody fragment capable of neutralizing the toxic effects of the whole venom from ... of mouse weight) [13] Clone 3F was matured by three cycles of directed evolution The use of a set of stringent conditions in the third cycle allowed the selection of several improved clones The ... Table shows the values obtained for the binding kinetic constants The affinity constants of both scFvs were similar, in the range of 10)7 m Affinity maturation Fig Specificity of phage-antibodies...

Ngày tải lên: 23/03/2014, 13:20

... ulcerations of the soft tissue of the skin respectively (15,16) M leprae on the other hand is well known for its association with the historical disease leprosy (17) However, there is a large group of ... development of monoclonal hybridomas, it required refolding of the antibody from the insoluble protein fraction (121) Instrumental for the expression of soluble antibody fragments was the development of ... months of antibiotic therapy) and the lack of specificity for active TB for diagnosis on the basis of symptomology, X-rays or TST/IGRAs alone (35) This is evidenced by the low proportion of microbiologically...

Ngày tải lên: 10/09/2015, 09:05

Ngày tải lên: 21/02/2014, 16:20

... used to guide the alignment Starting from the bottom right entry of the matrix, we go to the matrix entry from which the value of the current cell is derived in the recursion of the dynamic programming ... Milan Theater”, the IOB value for “I” is B-NP since it marks the beginning of a nounphrase (NP) On the other hand, “Theater” has an IOB value of I-NP because it is inside a nounphrase (Milan Theater) ... V5 The results support both our hypotheses For Hypothesis I, we see that the performance of the two syntactic alignments was higher than the nonsyntactic versions In particular, Version outperforms...

Ngày tải lên: 31/03/2014, 01:20

... participated in the cutting and staining of Page of the bone tissue RGR participated in the planning of the experiments, data analysis and the preparation of the manuscript MJS supervised the study ... the preparation of the bone cores and exposure of the samples to the different treatments, performed the microscopic analysis and helped with the planning and preparation of the manuscript PIF ... treatment for the generation of an acellular bone explant control to be used within an operating theatre is to our knowledge the repeated use of freeze-thawing cycles This method is one of the classical...

Ngày tải lên: 20/06/2014, 04:20

... in the project of more than to The majority of the staff producing biofertiliser in the project were women and the Vietnam Women’s Union played a strong role in providing acceptance of the technology ... already established between the Biofertiliser Laboratory at Hanoi University of Science and the SUNFix Centre for Nitrogen Fixation at the University of Sydney The advantage of the participants being ... research on development of quality control procedures) were based on the need to help guarantee the success of the technology and to reduce the risk of failure A considerable network of participants...

Ngày tải lên: 22/06/2014, 13:20

... addition, the algorithm should work well, regardless of the positive definiteness of the covariance matrices Furthermore, the algorithm should provide a straightforward method for the generation of complex ... on the variance of the complex Gaussian random variables at the inputs of Doppler filters as well as the characteristics of those filters The variance value of the outputs is then input into the ... powers of Rayleigh envelopes denoted as σr2j are not necessarily equal to one another Therefore, the scenario where the variances of the Rayleigh envelopes are equal to one another and the powers of...

Ngày tải lên: 23/06/2014, 00:20

... construct the equations of the continuous dynamical system with the exclusive use of the topological information from the network, the equations have to incorporate a set of default values for all the ... (see for example [6,7]) In these cases, the directionality of signaling is sufficient for developing mathematical models of how the patterns of activation and inhibition determine the state of ... of the networks might reflect the lack of a full characterization of the IFN-γ signaling pathway at the time of writing the corresponding papers It is important to note that the failure of these...

Ngày tải lên: 13/08/2014, 23:20

... of a substance in solution, t is the time, dC/dt is the PT dissolution rate, D is the diffusion coefficient of the substance, S is the surface area of RI the solid, V is the total volume of the ... flurbiprofen powder due to transformation of the drug from a crystalline to an amorphous form and the reduction of the size to the nanoscale When these NPs were orally administered to rats, the AUC of ... particles with the desired properties In the latter case, the advantage of water is associated with the non-toxicity and the reduction of the risk of explosion [1,114] Furthermore, mixtures of water...

Ngày tải lên: 02/09/2015, 13:31

... co-localisation of cells with the endothelial network is a pericyte specific behaviour Further we analysed the ability of cells to stabilise the formed endothelial network Figure 1-5: Tubular network formation ... knockout of the PDGF-B chain will therefore also eliminate the signalling of PDGF-B/PDGFR-α having further effects on organs like the heart (Leveen et al 1994) A common factor in both knockouts was the ... reduced in wounds of treated animals and the overall number of pericytes decreased (Rajkumar et al 2006) Therefore pericytes seem not to be necessary for the initial formation of blood vessels...

Ngày tải lên: 09/09/2015, 10:13

Ngày tải lên: 11/09/2015, 09:56

... Mechanism of 14C10 mAb 153 7.9 ADE profiles of 14C10 mAb 155 7.9.1 Development of ADE among the DV serotype 155 7.9.2 Development of ADE among the antibody subclasses 157 7.10 Protective capacity of ... optimization of EBV immortalized human B cell lines 94 3.1 Optimization of transformation of B cells with EBV 94 3.2 Optimization of cloning of EBV immortalized B cells 95 3.3 Markers of immortalized ... isolated the genes that form the template for this antibody and successfully generated a Fab (for X-ray crystallography) and various human IgG subclasses (IgG1, IgG2, IgG3 and IgG4) of recombinant monoclonal...

Ngày tải lên: 11/09/2015, 09:56

... triggers dissociation of the prM/E heterodimers, which leads to the formation of 90 dimers that lie flat on the surface of the particle, with prM capping the fusion peptide of the E protein This ... structure of the mature particle (Zhang, Corver et al 2003) The immature particles formed in the ER mature as they travel through the secretory pathway The slightly acidic pH (~5.8 - 6.0) of the trans ... the infection Based on the shape of the monomer and the location of the antibody epitopes, Rey et al postulated that the E protein has a „flat‟ topology along the surface of the virus lipid bilayer...

Ngày tải lên: 11/09/2015, 09:56

... harvesting the spleen cells Experiments with injected mice were performed under the guidelines for care and use of experimental animals 2.5 Cellular fusions When the anti-NPM-mA antibodies titre of mice ... Indirect ELISA was then carried out on NPM-wt and NPM-mA coated plates to check the antigenic characterization of mAbs Figure PCR amplifying the full sequence of ORF of the NPM-mA gene The PCR products ... Cloning of the recombinant vector pET-32a-NPM-mA A, Bacterial colony PCR for the detection of the BL21 (DE3) clones with the target prokaryotic expression vector pET-32a-NPM-mA 1-7: colonies of bacteria...

Ngày tải lên: 08/08/2014, 18:21

... site but at the bottom part of the C-terminal domain The only moderate effect of MalT on binding of 2F9 argues against these residues being part of the MalT–MalK interaction face Rather, the epitope ... Monoclonal antibodies recognize epitopes in the N-terminal (ATPase) domain and in the C-terminal domain of MalK, respectively Monoclonal antibodies were prepared against the MalK subunit of the ... in the absence of tertiary structural information on the complete transporter, these interactions are still poorly understood at the molecular level Here, we describe the use of monoclonal antibodies...

Ngày tải lên: 31/03/2014, 09:20

... 3) where C2 is the protein in the bulk, C10 is the concentration inside the gel, S and V1 are, respectively, the surface and the volume of film, V2 is the volume of the bulk, h is the massive exchange ... 24 h, there is a spread of the number of differentiated cells, being about 85% of the total cells In the second day, the number increased up to 90% and, in the third day, about 96% of the cells ... is the protein concentration on the surface of the film and finally ks is the desorption rate constant Substituting known values and by fitting the experimental data with the mathematical model of...

Ngày tải lên: 22/06/2014, 00:20

... to the possession of peritrichous flagella, which may indicate flagella specific antibodies are good probe for detecting Listeria The objective in this study is to generate the monoclonal antibodies ... monitor the development of the antibody level using the yolk diluted 1,000 times with PBST The yolk antibody also showed no cross reaction to any other bacteria (Table 1), and the antibody levels of ... polyacrylamide gel Production of monoclonal antibodies Monoclonal antibodies to flagellin were produced by immunizing BALB/c mice against 50 µg/ml of flagella and fusing equal numbers of splenic lymphocytes...

Ngày tải lên: 07/08/2014, 18:21

... cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni retfA ... rof yllacipocsorcim denimaxe saw llew hcaE syad rof 2OC %5 rednu ,Co73 ta detabucni erew setalp ehT llew/sllec 000,02 fo tnuoc llec lanif a ot erutxim eht ot dedda erew sllec 401FT eht ,h rof ... emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ares detavitcani eht htiw tset NS ehT evitisop...

Ngày tải lên: 07/08/2014, 18:21

... extended to the technical staff that assisted in the conduct of the studies over the past years and to Betty Davis for recording information into the TKP The studies were supported in part by the Department ... were collected for further analysis and the cells cryopreserved Since there was limited information on the pattern of reactivity of known LDM expressed on rabbit leukocytes, all hybridomas producing ... respectively) The level of expression of the LDM detected with MRB102A on lymphocytes was higher than Monoclonal antibodies specific for rabbit leukocyte differentiation molecules Table Reactivity of monoclonal...

Ngày tải lên: 07/08/2014, 20:23

Ngày tải lên: 09/08/2014, 01:22