hplc and column liquid chromatography

... ắc k ý h ấp ph ụ ( NP - HPLC va RP - HPLC) ; s ắc k ý phân b ố - s ắ c k ý chi ết ( LLC); s ắc k ý trao đổi ion ( IE - HPLC) ; s ắ k ý rây c phân t - s ắc k ý gel ( IG - HPLC) Nh th ự t ế hi ện ... hi ệt gi ảm đau Phần II : Hệ thống HPLC I- Máy HP:C g ồm b ộ ph ận c b ản đượ c tóm t s đồ sau : Column Degasse Pump Tiêm mẫu Detector Phần II: Hệ thống HPLC Trong : 1- Bìn h ch ứa dung môi ... m ềm nh ận,tín hi ệu s l ý d ữ li ệu ều ển h ệ th ống HPLC 8- In d ữ li ệu Phần II: Hệ thống HPLC II.1 - Bình đựng dung môi - Hiện máy HPLC thường có 04 đường dung môi vào đầu bơm cao áp Cho...

Ngày tải lên: 22/03/2014, 18:20

... solutions 3.12 Calibration Standard (CAL) A solution prepared from the primary dilution standard solution or stock standard solutions and the internal standards and surrogate analytes The CAL ... rules and land disposal restrictions The laboratory using this method has the respons-ibility to protect the air, water, and land by minimizing and controlling all releases from fume hoods and ... DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID- SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/ MASS SPECTROMETRY 1.0 SCOPE AND APPLICATION 1.1 This is a general purpose method...

Ngày tải lên: 22/03/2014, 14:20

... Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography- electrospray ... filtered, dried, and reconstituted into ml of methanol and water (85:15) The reconstituted solution was then filtered before HPLC injection Results and discussion Nucleosides and nucleobases as ... cytidine, and thymine [10,11,17,18]; maleic acid and succinic acid derivatives [6,19,20]; benzenoids [21]; phenol and tocopherols [8,22]; 5’-nucleotides [14]; and diterpenes [23] No chemical standardization...

Ngày tải lên: 13/08/2014, 15:21

... both the BAP31–Flag and the endogenous BAP31 proteins, migrating at apparent masses of 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9] For ... procaspases and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl) polymerase and endogenous BAP31 In contrast, cytoplasmic membrane blebbing and ... modifications Peptide mapping by capillary liquid chromatography microelectrospray tandem MS (LC-lESI-MS/MS) Ó FEBS 2003 BAP31 associates with c-actin and myosin B (Eur J Biochem 270) 345 Fig SDS/PAGE...

Ngày tải lên: 20/02/2014, 23:20

Ngày tải lên: 09/03/2014, 18:55

... using liquid chromatography coupled to electrospray tandem mass spectrometry Journal of Chromatography A 1157 (2007) 115-121 Guillermina Font Ana Juan-Garcia, Yolanda Pied - Pressurized liquid ... regulation 2002/657/EC, Journal of Chromatography A 1032 (2004) 2329 Che-Yi Lin, Shang-Da Huang - Application of liquid- liquy microextraction and high performance liquid chromatography for the determination ... and sulfamethoxazone (SMX) in animal foodstuffs (for fish, shrimp, chicken and pig) and food products (frozen shrimp, chicken liver and pig liver) The proposed conditions are used for rapid and...

Ngày tải lên: 20/03/2014, 21:20

... internal standard and b) without internal standard Figure the corresponded product the deuterated standards with Standard chromatogram of ion scans Standard chromatogram of the deuterated standards ... [16] Liquid chromatography Liquid chromatography separation was performed on a Hewlett-Packard HP 1100 series HPLC system equipped with a binary gradient pump, an isocratic pump, degasser and ... Leukotrienes LTB4 and LTE4as analytical standards and the labelled [2H4] LTB4 and [2H4] PGE2 as internal standards were purchased from Cayman Chemicals Company (Michigan, USA) All analytical standards...

Ngày tải lên: 20/06/2014, 00:20

... chromatography Nobel lecture, December 10 Alexander Efimov, 2005 HPLC introduction 11 Hans Neurath The Protein - Composition, Structure, Function 2nd edition Volume Academic Press, New York and ... Doudoroff, and Microbial World Prentice-Hall, Inc p.88-90 Edward A Adelberg The 39 13 Thomas D Brock Biology of microorganism Pretice-Hall, Inc p.684-681 14 Arthur T Henrici, M.D A handbook for ... handbook for students of Bacteriology Molds, Yeast, And Actinomycetes John Wiley & Sons, Inc, New York p.7-9 15 Zakia Bano, K.S Srinivasan, and H.C Srivastava, 1963 Amino acid composition of the...

Ngày tải lên: 28/07/2014, 06:20

... mẫu đƣợc lọc qua phận lọc đƣờng kính 20 µm để chuẩn bị cho phân tích HPLC 3.3.3.3 Phân tích HPLC Quá trình thực phân tách HPLC đƣợc thực với: Buffer A: sodium acetate TEA, pH 6,8 Buffer B: 60% ... với heptane (Thomas T Andersen, 1995) Với điều kiện phòng thí nghiệm tiến hành phƣơng pháp thổi khô với khí trơ Nếu PITC không đƣợc loại thải tốt làm hƣ nhƣ phá hủy cột HPLC - Mẫu đƣợc pha loãng ... phân cắt thành amino acid đơn (Hình 2.10) 3.3.3.2 Tạo dẫn xuất Khi phân tích thành phần amino acid HPLC, mẫu phải đƣợc tạo dẫn xuất với PITC để phát thông qua detector Quá trình tạo dẫn xuất đƣợc...

Ngày tải lên: 28/07/2014, 06:20

... thông qua UV (Ultraviolet) 2.4.3 High Performance Liquid Chromatography (HPLC) HPLC đƣợc đời vào đầu năm1970 công cụ phân tích đầu tay nhà phân tích HPLC dựa vào pha động (dạng lỏng) để phân tách ... (paper chromatography) , sắc ký lớp mỏng (thin layer chomatographyTLC), sắc ký khí (gas chromatography) , sắc ký trao đổi ion (ion exchange chromatography) , sắc ký lỏng cao áp (high performance liquid ... đến tạo dẫn xuất precolumn đƣợc ứng dụng rộng rãi yêu cầu HPLC ngƣợc pha Kỹ thuật nhạy nên đòi hỏi lƣợng mẫu phân tích nhỏ khoảng 0.5-1 µg Phƣơng pháp phát ninhydrin postcolumn Phƣơng pháp sắc...

Ngày tải lên: 28/07/2014, 06:20

... 13 iv 2.4.1 Sắc ký giấy (Paper chromatography) 13 2.4.2 Sắc ký lớp mỏng ( Thin layer chromatography _TLC) 14 2.4.3 High Performance Liquid Chromatography (HPLC) 15 2.4.3.1 Pha tĩnh ... THAM KHẢO 36 vi DANH SÁCH CÁC CHỮ VIẾT TẮT ACN:Acetonitril HPLC: High performance liquid chromatography TLC: Thin layer chromatography PITC: phenylisothiocyanate PTC: phenylisocarbamyl ... LOÀI NẤM BÀO NGƢ (Pleurotus spp.) BẰNG KỸ THUẬT SẮC KÝ LỎNG CAO ÁP (High performance liquid chromatography - HPLC) Giáo viên hƣớng dẫn: Sinh viên thực hiện: TS LÊ ĐÌNH ĐÔN ĐOÀN BẢO QUỐC Thành phố...

Ngày tải lên: 28/07/2014, 06:20

... (H)-pyran-4-one and 5-hydroxymethyl furfural; P-RPM contains less amino acids and monosaccharides and has a lower pH value than R-RPM [31] In recent years, high performance liquid chromatography (HPLC) and ... raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry Chinese Medicine 2010 5:29 Submit your next manuscript to BioMed Central and ... (Labscan, Thailand) was used for preparation of standards and sample extraction Reference compounds of 2,3,5,4′-tetrahydroxystilbene-2-O-b-D- glucopyranoside (THSG, 1), emodin (2) and physcion...

Ngày tải lên: 13/08/2014, 15:21

Ngày tải lên: 14/11/2014, 08:17

... contained in samples Sample Plant sources Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Wine Wine Wine Beverage Barbary ... acetate and formic acid, triethylamine Mobiles used for HPLC were filtered (0.45 ␮m) and ultrasonically degassed before use 2.2 Preparation of standards Stock solutions of sildenafil, vardenafil and ... fluid products such as wine, beverage, and oral liquid formulation, etc Hence, eight liquid products for examination (five oral liquid formulation, two wines, and one beverage, their herbal constituents...

Ngày tải lên: 02/09/2015, 13:06

... factors and external factors (Gibson and Skett, 1994) Internal factors involve species, genetics, age, sex, hormones (e.g pituitary gland, sex glands, adrenal glands, thyroid gland, pancreas and ... urine and feces Chiral centers are indicated with asterisks xiii LIST OF SYMBOLS CML chronic myelogenous leukemia HPLC high performance liquid chromatography UPLC ultra performance liquid chromatography ... urine, feces collection and sample preparation……111 5.2.3 Chromatography and mass spectrometry conditions for rat plasma and feces………………………………………………………113 5.2.4 Chromatography and mass spectrometry...

Ngày tải lên: 14/09/2015, 14:08

... water, drinking water, rain water, river water and seawater under various pH and irradiation sources (sunlight, darkness, indoor incandescent lighting and artificial UV lighting) Hydrolysis was found ... half-lives and degradation rates were obtained in various matrix and irradiation sources Based on kinetic studies, propoxur was found to degrade more rapidly at higher light exposure and pH Furthermore, ... analyze the residues of N-methylcarbamate and related compounds (propoxur and its metabolisms as model compounds) in biological materials (goldfish, tortoises and sea lettuce) Orthogonal array design...

Ngày tải lên: 16/09/2015, 17:13

... calculated (see Table 5-3, 5-4 and 5-6) For each tested species, two-variable interactions were assigned to column Furthermore, columns and were treated as dummies and used for estimating the error ... 80 °C extraction temperature, and 6-min microwave heating The differences between the distribution of propoxur and its transformation products in biota and soils, and additionally the difference ... the main variables (A, B, C and D), two-variable interaction, and the level setting values of the main variables applied to aquatic animals (goldfish and tortoises) and sea lettuce are displayed...

Ngày tải lên: 16/09/2015, 17:13

... pressure and heating duration 74 4.1.2 Experimental 4.1.2.1 Reagents and soil preparation Analytical-grade PAHs (naphthalene and phenanthrene), PCB (PCB-1242 and PCB1248), triazine (atrazine 98% and ... (S+1) x S (row × column) matrix, whose column number corresponds to the number of factors, and row number to the number of experimental trials The intersections between the columns and rows indicate ... AB AC and BC which, as indicated by their names, are interactions between A and B, between A and C, and between B and C, respectively Only two-factor interactions are considered since experience...

Ngày tải lên: 16/09/2015, 17:13