how cancer cells say no to cell death
Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
Ngày tải lên :
16/02/2014, 09:20
... MPyV minor proteins: inducers of cytotoxicity S Huerfano et al Fig Cytotoxicity of VP2 or VP3 fusion proteins Cytotoxicity of individual protein variants transiently expressed in 3T3 cells was ... transient expression of the minor structural proteins in permissive cells induces cell death; however, the cytotoxicity caused by their expression decreases when minor proteins are fused with EGFP ... proteins or EGFP only, mock-transfected cells, cells treated with actinomycin D (ActD), or untreated cells (A) Cleavage of caspase and of PARP in lysates of cells collected h post-transfection Western...
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Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death docx
Ngày tải lên :
16/03/2014, 04:20
... are shown (D) IVM analysis showing the intracellular distribution of CD47 molecule in zymosan-treated cells (central panel) and OPZ-treated cells (right panel) (E) DIC (Nomarski) micrographs showing ... shown Numbers represent the percentages of annexin V-positive cells (bottom right quadrant) or annexin V ⁄ trypan blue double positive cells (upper quadrant) Note the very low percentage of cells ... immunoreceptors [21], OPZ induced non-apoptotic externalization of PS Furthermore, OPZ-treated platelets dis not show either caspase activation or an increase in Bcl-2 family proteins, nor cell death...
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Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death potx
Ngày tải lên :
16/03/2014, 04:20
... neither wild-type nor D40G chitosanase are able to cleave GlcNAc–GlcNAc bonds (data not shown) The stereochemistry of the D40G chitosanase reaction was investigated by 1H-NMR As shown in Fig 3, ... of the V148T chitosanase-expressing cells and chitosanase-negative cells and plating them on media with various salt compositions Both colonies could be distinguished thanks to supplementation ... 5-bromo-4-chloro-3-indolyl-b-d-galactopyr- Active site residues of family 46 chitosanase anoside and isopropyl thio-b-d-galactoside, because the empty pAlter-1 vector directs b-galactosidase synthesis in E...
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Hypersensitivity to cell death under glucose starvation involves oxidative stress and AMPK instability
Ngày tải lên :
22/10/2015, 21:19
... caspases and cell death (Long and Ryan, 2012) The extrinsic apoptotic pathway is initiated by the binding of death ligands to death receptors, such as tumor necrosis factorα (TNFα) to TNFα receptor (TNFR1), ... 2012) 1.3 Programmed cell death Programmed cell death (PCD) is a controlled cellular mechanism for clearance of damaged and disordered cells to maintain tissue homeostasis and normal physiological ... are as follows: To study cell death in response to glucose starvation in NCI-H460 cells; To investigate the role of AMPK protein stability in glucose starvation-induced cell death The present...
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Báo cáo sinh học: "The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" pdf
Ngày tải lên :
18/06/2014, 19:20
... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) , medium (M -cells) and high (SM -cells) bioenergetic signatures Cells ... FL1-H 10 10 C Necrotic cell death (fold change vs control) G cells M cells Apoptotic cell death (fold change vs control) SM cells B 30 20 10 BrP IA 5-FU 15 10 BrP IA FU G- cells ns 0.9 0.6 0.3 ... with the cell- death response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) (A), medium (M -cells) (B) and high (SM -cells) (C) bioenergetic signatures...
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báo cáo hóa học:" The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" ppt
Ngày tải lên :
20/06/2014, 03:20
... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) , medium (M -cells) and high (SM -cells) bioenergetic signatures Cells ... FL1-H 10 10 C Necrotic cell death (fold change vs control) G cells M cells Apoptotic cell death (fold change vs control) SM cells B 30 20 10 BrP IA 5-FU 15 10 BrP IA FU G- cells ns 0.9 0.6 0.3 ... with the cell- death response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G -cells) (A), medium (M -cells) (B) and high (SM -cells) (C) bioenergetic signatures...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... radiation-induced apoptotic cell death in human cervical cancer cells Inhibition of JNK effectively protected cells from radiation-induced loss of mitochondrial membrane potential and apoptotic cell death FEBS ... kinetics of the apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic cell death by fluorescence ... isothiocyanate (FITC) is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptotic cell death For the cell- death assessment, the cells were plated...
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Báo cáo Y học: Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine pdf
Ngày tải lên :
17/03/2014, 23:20
... DENSPM-treated cells were in fact detached and the cells were difficult to discern due to fragmentation At 72 and 96 h after treatment, it was not possible to detect any intact cells All cells were ... alternative Another important property of an anticancer treatment is to minimize the damage to normal cells DENSPM and other polyamine analogues may have different toxic effects on normal cells and cancer ... not shown) Addition of Z-VAD.FMK to DENSPM-treated cells decreased the percentage of cells in the sub-G1 region to Fig The effect of DENSPM treatment on the polyamine content of L56Br-C1 cells Cells...
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Ruta 6 selectively induces cell death in brain cancer cells but proliferation in normal peripheral blood lymphocytes: A novel treatment for human brain cancer doc
Ngày tải lên :
22/03/2014, 17:20
... 981 Ca3(PO4)2 treatment is mitogenic and nonclastogenic in normal cells but antimitotic and apoptogenic in human MGR1 glioma cancer cells How glioma brain cancer cells are killed or checked from ... cytotoxic to human and murine cancer cells, it is more damaging to human glioma brain cancer cells than to HL-60 leukemia cells (data not shown); b) Ruta induces cell division in normal human PBLs when ... Ruta treatment induced apoptosis in human brain cancer cells and protected B-lymphoid cells from apoptosis, we subjected MGR1 cancer cells, normal B-lymphoid cells exposed to Ruta 6-high dose, added...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
Ngày tải lên :
09/08/2014, 09:20
... apoptotic cells, B apoptotic and necrotic cells, C normal cells and D necrotic cells The percentage of apoptotic and necrotic cells increased from 8% and 14% in control normoxic and hypoxic cells ... ability of colon cancer cells and is non-toxic to normal intestinal cells We have shown previously that DCQ is not toxic to normal mouse intestinal Mode K and IEC-6 cells [9] or to normal mouse mammary ... DCQ is not cytotoxic to normal intestinal cells DCQ at concentrations of up to 10 μM did not reduce FHs74 Int human normal intestinal cell viability At 50% confluency, cells were exposed to DCQ...
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Báo cáo khoa học: "The Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation" pot
Ngày tải lên :
09/08/2014, 09:20
... sufficient to overcome the cellular death threshold and to induce apoptotic death of LNCaP cells [16,36] In PC3 -cells, ionizing radiation also decreased cellular Bcl-2 levels but ErPC3 did not reduce ... show that the Akt-inhibitor ErPC3 increases radiation-induced apoptosis in prostate cancer cells The most prominent combination effects were obtained in LNCaP cells that did not show any apoptosis ... in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor of Akt even in cells that are...
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Human telomerase reverse transcriptase (hTERT) overexpression modulates intracellular redox balance and protects cancer cells from apoptotic cell death
Ngày tải lên :
14/09/2015, 08:38
... anti cancer agents today In order to achieve these goals, exploitation of the intrinsic differences between normal and cancer cells could be a valuable approach to target cancer cells for cell death ... somatic cells telomerase activity is extinguished during embryonic differentiation, the highly proliferative cells such as stem cells, germ cells, activated lymphocytes, haematopoietic progenitor cells, ... reason for the tumor cells aversion for SODs This 20 difference in SOD activity in normal cells and cancer cells has been explored by several groups to help target cancer cells (Kobayashi et...
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Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
Ngày tải lên :
16/09/2015, 08:31
... with cells resistance to apoptotic cell death As described in the previous section, O2.- can block the Fas-induced cell death in tumor cells The regulation of tumor cells sensitivity to death ... susceptibility to cell death in NIH3T3 cells 51 Fig Level of NHE-1 expression correlates with NIH3T3 cells sensitivity to staurosporine-induced cell death 51 Fig Silencing of NHE-1 gene leads to increased ... response 48 to death triggers in NIH3T3 cells III.1.b Regulation of NHE-1 gene expression regulates cells response 52 to death triggers in Tumor cells III.2.a Superoxide mediated cell survival...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
Ngày tải lên :
16/02/2014, 09:20
... Response to epidermal growth factor receptor inhibitors in non-small cell lung cancer cells: limited antiproliferative effects and absence of apoptosis associated with persistent activity of extracellular ... epidermal growth factor receptor inhibitors and cisplatin on proliferation and apoptosis of oral squamous cell carcinoma cells Int J Oncol 30, 1469–1476 Ciardiello F & Tortora G (2001) A novel approach ... essential mediator of p53-dependent and -independent apoptotic pathways Cancer Cell 4, 321–328 Yu J & Zhang L (2003) No PUMA, no death: implications for p53-dependent apoptosis Cancer Cell 4, 248–...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
Ngày tải lên :
06/03/2014, 22:21
... particularly, clear cell adenocarcinomas, cervical squamous carcinomas, ovarian carcinomas, colorectal carcinomas, esophageal carcinomas, bladder carcinomas and non-small cell lung carcinomas CA9 is ... screening to correlate gene expression profiles with sensitivity of human cancer xenografts to anticancer drugs Cancer Res 62, 518–527 13 Pastorekova S, Zatovicova M & Pastorek J (2008) Cancer associated ... h, cells were treated with or without agent for 48 h Then, Hoechst33258 was used to detect apoptosis according to a standard procedure, a fluorescence microscope was used to observe apoptotic cells, ...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
Ngày tải lên :
07/03/2014, 00:20
... been shown to induce apoptosis in colon cancer cell lines [20,21] The ability of tumour cells to evade apoptosis is an enormous obstacle for effective treatment Consequently, strategies aiming to ... reduced cell viability and increased cell death compared to pCR3-empty 5-FU exposed cells (data not shown) This prompted us to create a stable miR-143 overexpression model by transfecting HCT116 cells ... et al miR-143 modulates 5-FU cytotoxicity A B Fig mir-143 overexpressing cells are more sensitive to 5-FU cytotoxicity Cells were exposed to either 1–10 lM 5-FU or no addition (control) for 72...
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Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells pdf
Ngày tải lên :
07/03/2014, 03:20
... luciferase Control, cells co-transfected with empty pcDNA3.1 vector or parental cells SGC7901, pGL-MDR vector and pRL-TK vector; PrPC, cells co-transfected with empty pcDNA3.1B/PrPC vector treated with ... was classified into five groups: 0, no tumor cells exhibiting IR; 1, < 25% of the tumor cells exhibiting IR; 2, 25–50% of the tumor cells exhibiting IR; 3, 50–75% of the tumor cells exhibiting ... IC50 values (lgÆmL)1) for anticancer drugs for gastric cancer cells Survival rates of gastric cancer cells treated with anticancer drugs were evaluated by the MTT assay as described in Experimental...
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Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx
Ngày tải lên :
07/03/2014, 16:20
... to allow the cells to survive However, it is possible to discern the type of response of each gene by modifying their expression and testing how this modification affects MTX cytotoxicity As tools ... levels in HT29 cells The overexpression of vimentin sensitized the cells towards MTX both in parental and resistant cells, respectively 699 Gene expression in MTX-resistant HT29 cells S Penuelas ... the overexpressed gene IMPDH2 in HT29-R cells, we were able to sensitize the resistant cells to MTX, thus showing the modulator effect of IMPDH inhibitors As IMPCH expression was also increased,...
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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
Ngày tải lên :
16/03/2014, 00:20
... normal cells such as T lymphocytes, isolated neurons and endothelial cells, or malignant cell lines such as human hepatoblastoma cells (HepG2), colon adenocarcinoma cells (HT-29) and breast cancer ... panels, living cells (LC); right lower panels, early apoptotic cells (EAC); left upper panels, necrotic cells (NC); right upper panels, late apoptotic cells (LAC) Non-stained cells served as ... the cell lines to treatment with DHEA C33A and CASKI cells were more responsive to DHEA, and HeLa cells were the most resistant This might be related to the malignant state of the cells HeLa cells...
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