high throughput whole mount in situ hybridization for genetic screening

... probes by in vitro transcription 55 2.14.2 Whole mount in situ hybridization for phenotype characterization 55 2.14.3 High throughput whole mount in situ hybridization for genetic screening ... were obtained from a moderate scaled ENU mutagenesis and genetic screening in our group, which was initiated in 2001 Outcross will be performed on those lines for maintenance as well as for genetic ... reverse genetic tool, its relatively large size and low fecundity make it difficult to be used in performing forward genetic screening The intrauterine development of mouse fetus is inconvenient for...

Ngày tải lên: 14/09/2015, 12:11

... farthing of ScBH (10)6 dilution) used in PMCA is able to produce large quantity of PK-resistant rPrP Therefore, we speculate that certain binding domains of pyridine nucleotides may exist within ... PK (PK+) indicates reactions containing ScBH before PMCA process Round numbers are given on top The molecular markers are indicated on the right input PrPSc (Fig 1A, lane in all gels) In the NBH ... of TSEs in animal models [17] Interestingly, we also find that the presence of NADPH enhances de novo PrPSc propagation in PMCA PMCA-derived PrPSc maintains a stable propagating capacity in normal...

Ngày tải lên: 07/03/2014, 03:20

... signal does not contain basolateral targeting information [48] Interestingly, gap junction plaques were found predominantly on the lateral membrane domain, even in cell lines expressing constructs ... within a PPXY motif, PPGY(283286), which is a ubiquitin ligase (NEDD4) binding site that is involved in internalization and degradation of Cx43 [47] To determine whether Y286 is involved in the ... Chtchetinin et al Basolateral sorting of Cx43 in MDCK cells individual cell lines are shown) For comparison, limited signal was detected in uninfected control cells (Fig 3D), and cells infected...

Ngày tải lên: 23/03/2014, 04:20

... of joint damage Evaluations of this kind should preferably be performed in a cohort of patients with early RA, for whom both radiographic and clinical data have been assessed in detail during ... respectively: for C2C, 10– 17% and 14%; for C1,2C, 5–14% and 13%; for CS846epitope, 4–12% and 12%; and for CPII, 11–18% and 16% The interassay coefficients of variance for all the assays determined for ... example, a relative increase of 6.4% in the joint space narrowing score during the next year caused by a one standard deviation increase in the C2C value Discussion The search for markers to predict...

Ngày tải lên: 09/08/2014, 07:20

... (arrows) involved in thrombus formation Tissue section in E was stained with iron staining; tissue section in F, G, H, and I were stained with H & E staining Elevated pAkt and eNOS expression in simvastatin ... and cells were incubated for 30 minutes at 4°C and unbound reagents were removed by washing Cells were then resuspended in fixing buffer (PBS containing 1%formaldehyde and 1% FBS) for flow analysis ... hypercoagulable state in certain time window thus result in re-stenosis or late thrombosis in MI patients Therefore, it is important to screen for high risk patients with chronic inflammation or increased...

Ngày tải lên: 10/08/2014, 05:21

... are recommendations for increasing sustainability in power planning (i) Agencies responsible for demand forecasting should focus attention on making accurate and realistic forecasts (ii) Governments ... in this SEA for the first time in the GMS, can be a useful tool for increasing energy security in PDPs The combination of increased energy efficiency and increased renewable energy in the power ... working, e.g., when the sun is not shining for solar power, and when the wind is not blowing for wind power Description of the Scenarios Unsurprisingly, under the energy efficiency scenario, installed...

Ngày tải lên: 08/09/2015, 23:32

... message, To inform, To explain, and To train • A powerful way – Presentation: An assumed skill • A skill managers expectd to have acquired without any formal training, • And without getting accurate ... accurate feedback Supporting Materials • Slide-shows not the heart of the presenation • For backup, not a script Training in presentation skills • Most people require some training to help develop ... presentation • • • • • • Using your voice effectively, Controlling the pace of delivery, Using effective body language, Structuring the presentation Formatting your slides well Coming across as confident...

Ngày tải lên: 27/06/2016, 10:30

... scores for individual words and the language model scores Because of space limitation, we omitted the special sentence beginning and ending symbols in the formula above More details can be found in ... conducted on three meetings, containing 244 sentences in total Participants were asked to read the original sentence and assign scores to each of the compressed sentences for its informativeness and ... The snippets may not contain all the words in a sentence query, but often contain frequently occurring phrases For example, “of course” can be found with high frequency in the snippets We collect...

Ngày tải lên: 23/03/2014, 17:20

... subxiphoid pericardial window with insertion of 32F drain for more effective drainage The chyle leak continued to diminish in volume over the next five days, without any drainage by day eight However ... one of minimal intervention with a dual strategy of decreasing lymph production and ensuring adequate protein intake to counter any effects of the potential hypoproteineamia As first line management, ... a temperature the following day to 39.5 degree Celsius A full septic screen was performed including blood cultures and the chyle was sent for microbiological analysis Initially, the patient was...

Ngày tải lên: 10/08/2014, 10:20

... phát kỳ vọng Clarida cộng (1998) đề xuất sử dụng quy tắc Taylor dạng forward-looking • E kỳ vọng • Ωt vector chứa thông tin sẵn có • πt+k lạm phát • yt+p sản lượng PHẦN PHẦN PHẦN PHẦN PHẦN Quy ... thực cộng với credit spead futures interest rate spead Biến credit spread xem báo tốt cho chu kỳ kinh doanh căng thẳng tài chính, thay đổi mức chênh lệch lãi suất (interest rate spread) hợp đồng ... số biến inflation dương => phản ứng ECB tỷ lệ lạm phát => ECB không tuân theo quy tắc tuyến tính Taylor Cột – Cột 14: mô hình hồi quy theo quy tắc tuyến tính Taylor dạng forward-looking, sử dụng...

Ngày tải lên: 21/06/2015, 23:44

... financial index Taylor rule with the financial index used in the linear estimations to used in the linear estimations to check whether, after controlling for check whether, after controlling for nonlinearities, ... bank increases the real rate in response to higher inflation, which exerts a stabilizing effect on inflation; on the other hand  In situations in which output is below its potential a decrease in ... monetary policy can indeed be described by a linear Taylor rule or, instead, by a nonlinear rule?  whether that rule can be augmented with a financial conditions index containing information from...

Ngày tải lên: 14/07/2015, 08:34

... in the linear estimation 2nd PURPOSE After controlling for nonlinearities, the ECB and other two central banks are still (or not) reacting to the information contained in that index INTRODUCTION ... decrease in the interest rate will have a stabilizing effect on the economy Specification and estimation of the linear Taylor rule The linear Taylor rule Defining: Inserting Eq (3) into (2) assuming ... estimation of the linear Taylor rule The linear Taylor rule Defining: Inserting Eq (3) into (2) assuming that the central bank is able to control interest rates only up to an independent and identically...

Ngày tải lên: 14/07/2015, 08:34

... quan hệ kinh tế cụ thể Như vậy, tổng hợp thông tin từ vài tài sản biến tài số có trọng số (weighted index) cho phép rút liên quan tới kinh tế cụ thể biến thời điểm thế, gom lại lượng thông tin nhiều ... tính tới thông tin mong đợi tương lai Vì vậy, tiếp tục thử nghiệm với quy tắc forward-looking Taylor khu vực EU Phương pháp GMM sử dụng để ước lượng hệ số quy tắc forward-looking Taylor có sử ... với hoạt động kinh tế khu vực EU, liệu ECB có phản ứng với điều kiện kinh tế quốc tế hay không Kết nghiên cứu cho thấy EU có xem xét tác động kinh tế toàn cầu định lãi suất Trong kinh tế mở, nỗi...

Ngày tải lên: 14/07/2015, 08:37

Ngày tải lên: 04/10/2012, 10:24

... Protocols for the SWHS were approved by the Institutional Review Boards of all institutes involved in the study Dietary intake and other exposure information Dietary intake was assessed through an in- person ... heterocyclic amines formed during cooking Nitrosamines formed during cooking may be toxic to pancreatic cells [21] Consumption of foods with a high content of nitrites and nitrosamines has been ... chronic diseases Tests for trend were performed by entering the categorical variables as continuous parameters in the models Finally, we investigated the joint effect of meat intake categories and...

Ngày tải lên: 31/10/2012, 16:49

... important role in enforcing anti-dumping law of the EU It is responsible for receiving complaint, initiating an investigation, carrying out the investigation, imposing the provisional anti-dumping measures, ... price, in the ordinary course of trade, for the like product when destined for consumption in the exporting country Therefore, by imposing antidumping measures against foreign exporters, importing ... Trade is in charge of enforcing anti-dumping law It includes about 100 offices specializing in investigating dumping cases and other trade compensation measures  Ministerial Council The Ministerial...

Ngày tải lên: 04/04/2013, 16:17

... Taking notes input and output Summarizing Highlighting a Guessing intelligently Using linguistic clues Using other clues b Overcoming limitations in Switching to the mother speaking and writing tongue ... the following: Class Set of strategies Specific strategies Grouping Associating/Elaborating a Creating mental linkage Placing new words into a context Using imaginary Semantic mapping Using key ... sounds and writing systems Recognizing and using formulas and patterns Recombining Practicing naturalistically b Receiving and sending Getting the idea quickly messages Using receiving II Cognitive...

Ngày tải lên: 05/04/2013, 15:56

... important role in enforcing anti-dumping law of the EU It is responsible for receiving complaint, initiating an investigation, carrying out the investigation, imposing the provisional anti-dumping measures, ... price, in the ordinary course of trade, for the like product when destined for consumption in the exporting country Therefore, by imposing anti-dumping measures against foreign exporters, importing ... of determining individual margins of dumping for any exporters or producers in the exporting country who have not exported the product to the importing country during the period of investigation,...

Ngày tải lên: 27/07/2013, 08:50

... You slept in class' You were late for lessons' 'You talk in class' 'You look out of the window.' You forget your homework.' Encourage the students to repeat in a emphatic way, stressing the negative, ... the group with students passing the pictures and saying the words Don't forget to put the pictures in the same order as the words Keep passing the pictures around getting faster and faster until ... accurately Unfortunately simple repetition can bore students Here are two ways to make drilling vocaulary items more interesting For the first one you write the words you want students to learn in large...

Ngày tải lên: 06/09/2013, 10:10