high medium and low performance liquid chromatography hplc mplc and lplc

... Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography- electrospray ... fingerprinting is simple and useful Thus, this study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC by using high- performance liquid chromatography coupled ... Quality evaluation of mycelial Antrodia camphorata using high- performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS) Chinese Medicine 2010 5:4...

Ngày tải lên: 13/08/2014, 15:21

Ngày tải lên: 09/03/2014, 18:55

... chromatography Nobel lecture, December 10 Alexander Efimov, 2005 HPLC introduction 11 Hans Neurath The Protein - Composition, Structure, Function 2nd edition Volume Academic Press, New York and ... Doudoroff, and Microbial World Prentice-Hall, Inc p.88-90 Edward A Adelberg The 39 13 Thomas D Brock Biology of microorganism Pretice-Hall, Inc p.684-681 14 Arthur T Henrici, M.D A handbook for ... handbook for students of Bacteriology Molds, Yeast, And Actinomycetes John Wiley & Sons, Inc, New York p.7-9 15 Zakia Bano, K.S Srinivasan, and H.C Srivastava, 1963 Amino acid composition of the...

Ngày tải lên: 28/07/2014, 06:20

... mẫu đƣợc lọc qua phận lọc đƣờng kính 20 µm để chuẩn bị cho phân tích HPLC 3.3.3.3 Phân tích HPLC Quá trình thực phân tách HPLC đƣợc thực với: Buffer A: sodium acetate TEA, pH 6,8 Buffer B: 60% ... với heptane (Thomas T Andersen, 1995) Với điều kiện phòng thí nghiệm tiến hành phƣơng pháp thổi khô với khí trơ Nếu PITC không đƣợc loại thải tốt làm hƣ nhƣ phá hủy cột HPLC - Mẫu đƣợc pha loãng ... phân cắt thành amino acid đơn (Hình 2.10) 3.3.3.2 Tạo dẫn xuất Khi phân tích thành phần amino acid HPLC, mẫu phải đƣợc tạo dẫn xuất với PITC để phát thông qua detector Quá trình tạo dẫn xuất đƣợc...

Ngày tải lên: 28/07/2014, 06:20

... phát thông qua UV (Ultraviolet) 2.4.3 High Performance Liquid Chromatography (HPLC) HPLC đƣợc đời vào đầu năm1970 công cụ phân tích đầu tay nhà phân tích HPLC dựa vào pha động (dạng lỏng) để phân ... (paper chromatography) , sắc ký lớp mỏng (thin layer chomatographyTLC), sắc ký khí (gas chromatography) , sắc ký trao đổi ion (ion exchange chromatography) , sắc ký lỏng cao áp (high performance liquid ... thông qua phát sau bị tách qua cột, sắc ký khí,, điện di mao quản, HPLC Tuy nhiên, phân tích amino acid, sắc ký lỏng cao áp (HPLC) thƣờng đƣợc sử dụng có nhiều ƣu điểm nhƣ: thời gian ngắn độ phân...

Ngày tải lên: 28/07/2014, 06:20

... 13 iv 2.4.1 Sắc ký giấy (Paper chromatography) 13 2.4.2 Sắc ký lớp mỏng ( Thin layer chromatography _TLC) 14 2.4.3 High Performance Liquid Chromatography (HPLC) 15 2.4.3.1 Pha tĩnh ... THAM KHẢO 36 vi DANH SÁCH CÁC CHỮ VIẾT TẮT ACN:Acetonitril HPLC: High performance liquid chromatography TLC: Thin layer chromatography PITC: phenylisothiocyanate PTC: phenylisocarbamyl ... MỘT SỐ LOÀI NẤM BÀO NGƢ (Pleurotus spp.) BẰNG KỸ THUẬT SẮC KÝ LỎNG CAO ÁP (High performance liquid chromatography - HPLC) Giáo viên hƣớng dẫn: Sinh viên thực hiện: TS LÊ ĐÌNH ĐÔN ĐOÀN BẢO QUỐC...

Ngày tải lên: 28/07/2014, 06:20

... ắc k ý h ấp ph ụ ( NP - HPLC va RP - HPLC) ; s ắc k ý phân b ố - s ắ c k ý chi ết ( LLC); s ắc k ý trao đổi ion ( IE - HPLC) ; s ắ k ý rây c phân t - s ắc k ý gel ( IG - HPLC) Nh th ự t ế hi ện ... m ềm nh ận,tín hi ệu s l ý d ữ li ệu ều ển h ệ th ống HPLC 8- In d ữ li ệu Phần II: Hệ thống HPLC II.1 - Bình đựng dung môi - Hiện máy HPLC thường có 04 đường dung môi vào đầu bơm cao áp Cho ... công tác xây dựng tiêu chuẩn Phần II: Hệ thống HPLC L ưu ý : - T ất c ả dung môi dùn g cho HPLC đề u ph ải dung môi tinh ết có ghi rõ nhãn dùn g cho HPLC Hay dung môi tinh ết phân tíc h - T ất...

Ngày tải lên: 22/03/2014, 18:20

... (H)-pyran-4-one and 5-hydroxymethyl furfural; P-RPM contains less amino acids and monosaccharides and has a lower pH value than R-RPM [31] In recent years, high performance liquid chromatography (HPLC) and ... raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry Chinese Medicine 2010 5:29 Submit your next manuscript to BioMed Central and ... R-RPM: raw Radix Polygoni Multiflori; P-RPM: processed Radix Polygoni Multiflori; HPLC: high performance liquid chromatography; MS: mass spectrometry; THSG: 2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucopyranoside...

Ngày tải lên: 13/08/2014, 15:21

... equipped with an ESI probe and quadrupole analyzer The control of system and data acquiring was performanced with Masslynx3.5 workstation (Waters) The standards of sildenafil and tadalafil were obtained ... contained in samples Sample Plant sources Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Oral liquid preparation Wine Wine Wine Beverage Barbary ... acetate and formic acid, triethylamine Mobiles used for HPLC were filtered (0.45 ␮m) and ultrasonically degassed before use 2.2 Preparation of standards Stock solutions of sildenafil, vardenafil and...

Ngày tải lên: 02/09/2015, 13:06

... gradient bands, HPLC analysis revealed that a- and b-phycocyanins Ó FEBS 2002 are the most abundant components Their presence is particularly prevalent in the heavier bands (bands 3–5), and it is ... band at the top The first band, corresponding to the smaller complexes, was faintly colored, the second was the most colored and the more abundant, whereas the other two bands, observed at a higher ... the higher density subcomplexes Each band was collected with a syringe and analyzed by RP -HPLC Besides being more efficient than SDS/PAGE in discriminating between the four allophycocyanins and...

Ngày tải lên: 08/03/2014, 16:20

... herbs and material using microscopic identification method Instruments and conditions High- performance liquid chromatography- diode array detection (HPLC- DAD) analysis was performed on a liquid chromatography ... mass spectrometry; LC-ESI/MS: liquid chromatography- electrospray mass spectrometry; HPLC- DAD: highperformance liquid chromatography- diode array detection; MS/MS: tandem mass spectrometry; RDA: ... http://www.cmjournal.org/content/4/1/15 Figure Representative HPLC/ UV chromatograms of reference standards (A) and DCT (B) Representative HPLC/ UV chromatograms of reference standards (A) and DCT (B) The retention times of...

Ngày tải lên: 13/08/2014, 15:21

... of standard chromatographic techniques such as thin layer chromatography, gas chromatography, capillary electrophoresis, flow injection analysis, and high performance liquid chromatography (HPLC) ... histamine standard and samples (A), (C) Histamine standard (1 µg/ml); (B), (D) tuna sample at day of storage time (A) and (B): chromatography derived by benzoylation procedure; (C) and (D): chromatography ... performance liquid chromatography" has been carried out Thesis objectives: - Selecting the appropriate solvent for extraction process and analysis procedure to determine histamine in seafoods by high performance...

Ngày tải lên: 06/10/2015, 13:05

... regulation 2002/657/EC, Journal of Chromatography A 1032 (2004) 2329 Che-Yi Lin, Shang-Da Huang - Application of liquid- liquy microextraction and high performance liquid chromatography for the determination ... using liquid chromatography coupled to electrospray tandem mass spectrometry Journal of Chromatography A 1157 (2007) 115-121 Guillermina Font Ana Juan-Garcia, Yolanda Pied - Pressurized liquid ... residues in meat Journal of Chromatography A 1159 (2007)233-241 SUMMARY DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID CHROMATOGRAPHY PART SEPARATION AND QUANTIFICATION SULFAGUANIDINE,...

Ngày tải lên: 20/03/2014, 21:20

... 500 pL of i M acetic acid Repeat the last step and combine the acetic acid eluates Inject 20 ~L into the liquid chromatograph HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF URINARY CATECHOLAMINES ... Circulating and urinary catecholamines in pheochromocytoma N e w EngL J Me& 301,682-686 (1979) Gitlow, S.E., Mendlowitz, M., and Bertain, L The biochemical techniques for detecting and establishing ... Galen, R.S and Gambino, S.R Beyond Normality: The Predictive Value and Efficiency of Medical Diagnosis John Wiley and Sons, New York, NY (1975), 62-74 Soldin, S.J and Hill, J.G Simultaneous liquid- ...

Ngày tải lên: 16/12/2012, 15:24

... Protein bands were visualized by Coomassie blue staining Bands of interest were excised from the gel and proteins were digested in-gel following a published protocol [12] modified as follows Briefly, ... both the BAP31–Flag and the endogenous BAP31 proteins, migrating at apparent masses of 33 kDa and 28 kDa, respectively, and two additional protein bands at apparent masses of 42 and 190 kDa [9] For ... procaspases and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl) polymerase and endogenous BAP31 In contrast, cytoplasmic membrane blebbing and...

Ngày tải lên: 20/02/2014, 23:20

... by digestion with NcoI and PstI This vector backbone was excised from an agarose gel and purified For both standard and error-prone PCR amplification of hMnSOD genes, the following primers were used: ... N73S–Q143A hMnSOD has a low level of product inhibition, similar to that of the parent mutant Q143A hMnSOD, while exhibiting higher catalytic activity and efficiency Although even higher catalytic activity ... variants of hMnSOD with high catalytic activity and low product inhibition Such hMnSOD variants could be useful agents in cancer therapy Experimental procedures Reagents and kits All plasmids from...

Ngày tải lên: 07/03/2014, 11:20

... still expanding at the fast rate of 3,436 km2 a year In addition, arable lands are decreasing by 300 to 600 thousand hectares yearly and deterioration of soils is advancing Natural grasslands are ... supply IEEJ: March 2003 and demand policy measures, a poorly functioning environmental control system, lack of supervising ability at both administrative and citizen levels, low and inconsistent environmental ... “Reforms and Opening” policies and achieved a high economic growth of around 10% yearly As a result, China became an economic power, with its nominal GDP ranking sixth in the world and its export...

Ngày tải lên: 31/03/2014, 05:21