h 1 of simply connected groups by m kneser

... 80 12 0 16 0 200 10 0 10 1 10 2 FL2 -H 10 3 10 4 B 10 3Q 10 3Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 0 10 1 10 2 FL2 -H 10 3 10 4 Fig Analysis of mitochondrial membrane potential (DYm) and morphology (A) DYm analysis ... either Hsp70 ⁄ Hdj -1 or Hsp27 induces protection against httEx1-polyQ-induced cell death [15 ,56] The Hsp70 ⁄ Hdj -1 chaperone machine acts by decreasing htt aggregation [39,40], whereas Hsp27, which ... httEx1-polyQ-mediated DYm disruption Transfections were performed with a combination of either httEx1-72Q-EGFP or httEx1 -10 3Q-EGFP vectors with those encoding Hsp70 ⁄ Hdj -1 and Hsp27 As in (A), httEx1 -10 3QEGFP-expressing...

Ngày tải lên: 19/02/2014, 06:20

... 1- (3¢,5¢-Dimethoxyphenyl) -1- hydroxy-2(4¢-methoxyphenyl)ethane This product was prepared by the Barbier reaction [36] To a mixture of magnesium turnings (1. 1 mg atom) and 3,5-dimethoxybenzaldehyde (1. 1 mmol) in anhydrous tetrahydrofuran (THF; 10 mL) ... Renganathan, V & Gold, M .H (19 89) Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium J Biol Chem 264, 14 185 14 1 91 29 Bao, W., Fukushima, ... from the white rot basidiomycete, Phanerochaete chrysosporium Arch Biochem Biophys 234, 353–362 11 Schoemaker, H. E (19 90) On the chemistry of lignin biodegradation Recl Trav Chim Pays-Bas 10 9,...

Ngày tải lên: 20/02/2014, 23:20

... come to him from the outside So true is this that one of the best means of mitigating or curing many ailments is to divert the child's attention from himself to things outside of himself that he ... than in their own bodies The normal, healthy child thinks very little of himself apart from the other boys and girls, the games, the studies, the animals, the nature wonders, the hardships that ... they cannot afford to turn aside from the three R's to teach hygiene, to admit that they have not hitherto identified the teaching of hygiene with the promotion of the physical welfare of children...

Ngày tải lên: 06/03/2014, 15:20

... containing 10 mm Tris ⁄ HCl (pH 7.4), 50 mm KCl, mm MgCl2, 1% Triton X -10 0, lgÆmL )1 aprotinin, lgÆmL )1 leupeptin, mm dithiothreitol, 0 .1 mm Na2VO4 and 0 .1 lgÆmL )1 Pefabloc SC (Roche Diagnostic, ... (0.06 gÆL )1) , l-lysine-HCl (0.09 gÆL )1) , l-methionine (0.02 gÆL )1) , magnesium chloride- 6H2 O (0.06 gÆL )1) , magnesium sulfate (heptahydrate) (0 .10 gÆL )1) , calcium chloride (0 .15 gÆL )1) , sodium bicarbonate ... eIF2a-holophosphatase complex containing PP1c [ 21] The exact mechanism by which Nck modulates eIF2aSer 51 phosphorylation, as well as its role in the holophosphatase complex, still remain to be defined In this...

Ngày tải lên: 07/03/2014, 05:20

... mutant from that of MR-1R cells, we calculated a decaheme cytochrome c content (OmcA + OmcB) in MR-1R of about 6.68 pmol per 10 9 cells This value matches the sum of both decaheme cytochrome c concentrations ... quantification of the outer membrane decaheme cytochromes c OmcA and OmcB The heme content of whole cells was determined using the difference absorption coefficient of 21 400 m )1 cm )1 [16 ] at 554 nm for the ... l-serine (1 lgÆmL )1) , l-arginine (1 lgÆmL )1) , l-glutamate (1 lgÆmL )1) , lactate (15 mm), and fumarate (20 mm) For growth experiments, fumarate was replaced by either Fe(III)-citrate (2 mm), Fe(III)-nitrilotriacetic...

Ngày tải lên: 07/03/2014, 09:20

... 79.2 1. 86 1. 80 2.40–2.30 2.07–2.00 1. 87 1. 81 1.87 1. 81 1.87 1. 81 2 .18 –2 .10 2.07–2.00 1. 97 1. 90 24.6 1. 81 19.0 21. 8 16 77 24.0–2.30 18 .1 20.0 16 62 24.6–2. 01 18.0 20.3 16 47 27.9 1. 81 18.8 222.4 16 97 ... 642.7 91 652.743 633.740 636.743 610 .705 663 .14 1 778.977 768.908 768.908 14 00.48 14 26.56 13 69 .16 13 94.59 13 98.39 13 91. 54 15 28. 61 1572 .11 15 51. 03 11 10 10 11 11 12 39 52 52 48 51 52 53 47 61 0.90 ... (°) P 212 12 0.920 P 212 12 1. 386 P 212 12 0.970 P 212 12 1. 375 P 212 12 1. 375 P 212 12 1. 375 P 212 12 0.970 P 212 12 0.958 P 212 12 0.958 59.04 86.83 46.98 1. 80 42993 16 613 69.7 9.4 63.0 9.4 59 .15 86.98 47 .16 2.30...

Ngày tải lên: 08/03/2014, 02:20

... (2 011 ) 2596–2608 ª 2 011 The Authors Journal compilation ª 2 011 FEBS A L Cavatorta et al 10 11 12 13 14 15 16 17 18 and differentiation of human intestinal epithelial cells J Biol Chem 279, 10 157 10 166 ... of mRNAs Genome Biol 3, REVIEWS0004 .1- 0004 .10 35 Lue RA, Marfatia SM, Branton D & Chishti AH (19 94) Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor ... mechanism by which the tumour suppressor activities of DLG1 may be regulated: downregulation of DLG1 by modulation of the relative expression of DLG1 5¢-UTRs Furthermore, having shown that these 5¢-UTRs...

Ngày tải lên: 22/03/2014, 16:20

... with IC50 values of 12 13 lm Characterization of the mechanism of HIV -1 RT inhibition by alizarine derivatives Because it has been reported that the HIV -1 RNase H activity might be inuenced by the ... > 10 0 (88%) > 10 0 (95%) KNA-26 > 10 0 (90%) > 10 0 (59%) K-52 > 10 0 (10 0%) 61 K-53 > 10 0 (10 0%) > 10 0 K-54 39 60 KNA-53 21 52 K -12 6 10 0 61 K- 61 > 10 0 ( 91% ) 14 K -11 1 > 10 0 (80%) > 10 0 (10 0%) ... 20 10 Compound concentraion ( M) 10 0 K049 B 200 RDS1643 RT +RT 32-mer 28-mer 24-mer 15 -mer C 0.060 10 11 12 13 14 0.20 Slope 0 .15 0.045 1/ V (1/ fmoles) RNA:DNA template utilized in the biochemical...

Ngày tải lên: 22/03/2014, 16:20

... 2008 The Authors Journal compilation ª 2008 FEBS 47 71 Protection of r32 from H B by kCIII 10 11 12 13 14 15 16 17 18 19 S Halder et al and other Escherichia coli proteins J Bacteriol 14 9, 10 50 10 63 ... Kochan J & Murialdo H (19 82) Stimulation of groE synthesis in Escherichia coli by bacteriophage lambda infection J Bacteriol 14 9, 11 66 11 70 Hoyt MA, Knight DM, Das A, Miller HI & Echols H (19 82) ... The protein mixture was then passed through a mL benzamidine column (Amersham) for removal of thrombin Finally, the native protein was separated from the mixture by treatment with Ni2+– nitrilotriacetic...

Ngày tải lên: 23/03/2014, 10:20

... washed with a 10 0-fold volume excess of 0.0 01 m HCl at 21 °C The resulting packed beads (0.3 mL) were mixed with mg of 6 -11 B -1 antibody (or mg of Pma1p antibody) in mL of coupling buffer (0.5 m NaCl ... compound Evidence for the involvement of membrane proteins Mol Cell Biochem 17 0, 91 98 14 Beltramo DM, Nunez M, Alonso A del C & Barra HS ˜ (19 94) The relationship of hydrophobic tubulin with membranes ... tubulin H+ -ATPase complex by glucose Malpartida F & Serrano R (19 81) Phosphorylated intermediate of the ATPase from the plasma membrane of yeast Eur J Biochem 11 6, 413 – 417 Capieaux E, Vignais M- L, Sentenac...

Ngày tải lên: 23/03/2014, 11:20

... 40, 50 mm TrisHCl pH 8.0) to obtain total cell lysate or with membrane buffer (10 mm KCl, 1. 5 mm Mg2Cl, mm EDTA, mm EGTA, mm DTT, 20 mm Hepes, pH 7.5) to obtain membrane fractions [75] Samples ... Analysis of signalling mechanism: Gai After pretreatment with PTX (10 0 ngÆmL )1, overnight) human primary dermal fibroblasts were cultured without or with hormone (10 0 nM ET -1, 18 0 pM TGF-b1) for ... of tissue inhibitor of matrix metalloproteinase -1 (TIMP -1) and inducing that of matrix metalloproteinase-2 (MMP-2) [7 ,16 ], which in turn degrades MMP -1 cleaved collagen I [17 ] Regulation of MMP-1...

Ngày tải lên: 23/03/2014, 11:20

... at room temperature [22,24,26,27] The uptake buffer was 25 mM 2-(N-morpholino)ethanesulfonic acid/tris(hydroxymethyl) aminomethane (Mes/Tris, pH 6.0) with 14 0 mM NaCl, 5.4 mM KCl, 1. 8 mM CaCl2, ... 215 .3 ± 18 .2 pmolÆ (10 min) )1 (mg protein) )1, SKPT: 2 41. 7 ± 32 .1 pmolÆ (10 min) )1 (mg protein) )1] was designated as 10 0% (n ¼ 4) Insets: Uptake rate of [14 C]Gly-Sar (10 lM, 10 min, pH 6.0) was measured ... (10 lM) In Caco-2 cells, [14 C]Gly-Sar flux values were 22.9 ± 0.2 pmolÆcm)2 h) 1 (¼ 0.09 ± 0.0 01% Æcm)2 h) 1) without and 19 .3 ± 0.3 pmolÆ cm)2 h) 1 with mM alafosfalin (Table 1) In SKPT cells, the...

Ngày tải lên: 23/03/2014, 12:20

... nigricillis venom: a proton NMR and molecular modeling study Biochemistry 31, 11 335 11 347 Ruan, K .H. , Stiles, B.G & Atassi, M. Z (19 91) The short neurotoxin binding regions on the a-chain of human and ... relationship of postsynaptic neurotoxins from snake venoms Pharmacol Ther 34, 403–4 51 Joubert, F.J (19 73) Snake venom toxins, the amino acid sequences of two toxins from Ophiophagus hannah Biochem Biophys ... a higher temperature, 37 °C, were used wherein the water signal was shifted relative to Ha resonances The results of the spin system identification were confirmed by the 1H- 13 C HSQC spectrum The...

Ngày tải lên: 23/03/2014, 13:20

... submucous-intramural or multiple intramural fibroids underwent miomectomy by minilaparotomy following hysteroscopy and chromopertubation The mean length of follow- up was 49 months (range: 11 to 11 8 months) ... reduction of post-operative adhesions following myomectomy Hum Reprod 2008 23(5) :10 93 -11 00 Mismer S.A.,Cramer D.W:The epidemiology of endometriosis Obstet.Gynecol Clin.North.Am 2003; 30 :1- 19 Murdoch, ... disorder (autosomal dominant) meaning that each child has a 50% chance of inheriting the disorder from a parent who carries the gene The gene can be inherited from either mother or father The exact...

Ngày tải lên: 23/03/2014, 14:20

... H. -T Li et al A B 11 5 15 N (ppm) 12 0 12 5 13 0 8.5 8.0 1H 7.5 (ppm) 7.0 8.5 8.0 1H 7.5 (ppm) 7.0 Fig 1H- 15 N HSQC spectra of the reactions of a-Syn with quinone and DA (A) Overlay of the 1H- 15 N HSQC ... (M) with molecular masses around 15 kDa are the contaminants of monomeric form in the dimeric fraction M: monomer, r .m. m of  14 460 Da; D: dimer, r .m. m of  28 920 Da The peaks with larger molecular ... 11 9 ¼ 14 460 +624 +32 +3 4 91 ¼ 14 460 +32–1a 14 460 14 460 + 10 4 n 14 460 + 10 4 n 14 460 + 16 m + 10 4 n 14 460 + 16 m + 10 4 n 14 460 +16 m No DA-adduct was detected in the monomeric form with a-Syn...

Ngày tải lên: 23/03/2014, 15:20

... Coincidentally, both genes are close to the 1p36 region on chromosome [9 ,10 ] Ghosh et al [11 ] reported that the C-terminal MBP -1 protein, which is highly homologus to ENO1, exhibited transcriptional ... with 10 lm MG -13 2, or untreated, for 24 h and then lysed in lysis buffer [50 mm Tris (pH 7.4), 15 0 mm NaCl, 0 .1% SDS, 1% Triton X -10 0, mm EDTA, protease inhibitor cocktail (Roche), 10 lm MG -13 2 ... protein (MBP -1) regulates prostate FEBS Journal 277 (2 010 ) 4308–43 21 ª 2 010 The Authors Journal compilation ª 2 010 FEBS J Lung et al 10 11 12 13 14 15 16 17 18 19 cancer cell growth by inhibiting MAPK...

Ngày tải lên: 29/03/2014, 21:20

... identification of transcription start site of human KCTD10 To determine the genomic structure of human KCTD10, a full-length cDNA sequence of human KCTD10 was used to search the human genome database The ... showed that human KCTD10 was mapped to chromosome 12 q24 .11 region, spanned  21. 3 kb and contained five exons Mouse and human KCTD10 share identical organization and exons with similar length ... bound with transcription factor Sp1 Sp1 helps to maintain the hypomethylation of CpG islands [13 ,14 ], and interacts with some components of the basal transcription complex [15 ,16 ]; therefore...

Ngày tải lên: 30/03/2014, 02:20

... the first time that I’ve seen the baby I suppose you’ll put him in the Orphan Home in the city ” “No, I shall nothing of the sort, ” was the emphatic reply “He shall stay here until his mother ... was what he had said about the Poor House, and his father and mother which worried him “What did he mean? ” he asked himself over and over again Why did he say that I should go to the Poor House ... that, Martha I not for a moment doubt that such a thing is possible, oh, no But that old shawl and those plain clothes not look much like heavenly robes, they? I think that the hands which made...

Ngày tải lên: 30/03/2014, 15:20

... After 12 h, the total amount of Ins (1, 4,5)P3 (1. 24 ± 0.24 pmol per 10 6 cells; n ¼ 4) was higher than the concentration measured in cell line as 11 (0.95 ± 0.05 pmol per 10 6 cells; n ¼ 4), which has ... G & Scharpe, S (19 95) The purification, characterization and analysis of primary and secondary-structure of prolyl oligopeptidase from Ó FEBS 2002 10 11 12 13 14 15 16 17 18 19 20 21 Ins (1, 4,5)P3 ... decrease the cleavage by proline-specific peptidases Biochim Biophys Acta 12 93, 14 7 15 3 44 Willams, R.S., Cheng, L., Mudge, A.W & Harwood, A.J (2002) A common mechanism of action for three mood-stabilizing...

Ngày tải lên: 31/03/2014, 08:20