gus gene expression in arabidopsis protoplasts

... Thirteen genes, which encode six protein families responsible for protein folding, are included in the UPR gene list Among them, BiP (three genes), calnexin (two genes), calreticulin (two genes), ... ERSE-like Xbp1 binding-like P-UPRE ERSE-like ERSE-like ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like – Xbp1 binding-like ... protein response genes in Arabidopsis vesicle trafficking ⁄ transport, vacuolar protein sorting, cell wall biogenesis, and lipid ⁄ inositol metabolism In comparison with those of yeast, the UPR genes...

Ngày tải lên: 07/03/2014, 21:20

... Comparison of gene expression in different nectary types Comparison of gene expression in different nectary types The number of genes displaying a two-fold or greater difference in expression in different ... examine gene expression in a wide variety of tissues, and under a broad set of conditions, in Arabidopsis (e.g., [40,41]) However, to date, no genome-wide information on gene expression in nectaries ... (p or q > 0.05) For a more in- depth analysis of genes displaying the largest differences in expression, lists of genes displaying fivefold or greater differences in expression level between MLN...

Ngày tải lên: 12/08/2014, 03:20

... the interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: ... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 ... oncogene, constant part of the λIg gene, the mitochondrial genes of NADH dehydrogenase subunit (ND4), the interferon-inducible gene 6-16 (Inf-ind), the 16S rRNA gene (most probably the humanin gene...

Ngày tải lên: 02/11/2012, 11:08

... the ordinates represent the linear ratio of differential gene expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed expression ... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... the genetic consequences of targeted gene therapy in vitro (and in vivo) Microarray Study Differential Gene Expression Differential gene expression profiling is routinely used to detect genes...

Ngày tải lên: 03/11/2012, 11:44

... incubation in medium containing fetal bovine serum In parallel to their effects on the cyclin D1 gene promoter, EPA and DHA also reduced the egr-1 gene promoter activity stimulated by the coexpression ... caveolin proteins [22] Several studies have shown that caveolin, a 21–24 kDa integral membrane protein, plays a critical role in the regulation of cell proliferation, including SMC, by inhibiting ... SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 seem to act as scaffolding proteins that negatively regulate...

Ngày tải lên: 19/02/2014, 16:20

... examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in eWAT Next, we analyzed the changes in uncoupling protein (UCP1) in ... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... also increased in mBAT in response to PM2.5 exposure We did not observe significant differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the gene expression of Dpt in...

Ngày tải lên: 06/03/2014, 19:20

... protein kinase kinase (MEK) inhibitor, U0126 [32,33] Therefore, the role of the MAPK pathway in regulating PDK mRNA expression was investigated by incubating myoblasts for h in the presence of insulin ... inhibitors of signalling pathways known to be activated by insulin were used Two distinct phosphatidylinositol 3-kinase (PtdIns3K) inhibitors, wortmannin and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyan-4-one ... used to examine the role of PtdIns3K in regulating PDK transcript abundance in response to insulin (Fig 2) Incubation with either LY294002 (50 lm) or wortmannin (100 nm) significantly inhibited...

Ngày tải lên: 07/03/2014, 17:20

... protein, and in consequence, to reduce the amount of Ab peptide released in neuronal cells We asked the question whether we can inhibit expression of the gene of BACE protein using our engineered ... pUC-KE-tRNACTE-Rz-2, encoding Rz-1 and Rz-2, respectively, were obtained on a multimilligram scale Constructs containing inactive versions of Rz-1 and Rz-2 were obtained in the same way by using templates ... this expression system is of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45] Studies on an inhibition of BACE gene expression by endogenously generated...

Ngày tải lên: 08/03/2014, 08:20

... transformed into RAS46 and recombinant strains carrying the chromosomally inserted plasmid (by means of single homologous recombination) were identified by their resistance to tetracycline and their inability ... binding protein (MBP) and NifL was synthesized in NCM1529, carrying the plasmid pJES794 [31], grown aerobically at 30 °C in maximal induction medium [32] supplemented with 0.5 mM riboflavin Expression ... Barrett, J., Dixon, R & Austin, S (2001) Protein– protein interactions in the complex between the enhancer binding protein NifA and the sensor NifL from Azotobacter vinelandii J Bacteriol 183,...

Ngày tải lên: 17/03/2014, 10:20

... significant increase in the expression of the cyclin-dependent kinase inhibitor p21, a target of p53 [10] Comprehensive gene expression profiling has revealed that the pattern of gene expression in CCA ... gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in the CCA-specific expression ... altering activity in MCAS cells, suggesting that this region contains an element involved in CCA-specific expression of ANX4 In this region, the presence of putative transcription factor-binding...

Ngày tải lên: 22/03/2014, 16:20

... differences in expression of genes encoding enzymes and other proteins involved in intermediary metabolism Perivenous expression is indicated by green, and genes with preferential periportal expression ... localized in the periportal area, namely ornithine transcarbamylase (EC 2.1.3.3), argininosuccinate synthetase (EC 6.3.4.5), argininosuccinate lyase (EC 4.3.2.1), and arginase (EC 3.5.3.1), showing ... Zonal gene expression in mouse liver A Braeuning et al enzymes of serine ⁄ glycine metabolism: Glycine decarboxylase, a component of the glycine dehydrogenase complex that converts glycine to serine,...

Ngày tải lên: 23/03/2014, 10:20

... two Cys2–Cys2 type zinc finger motifs that serve as interfaces in both DNA–protein and protein–protein interactions [47] The E domain is known to be involved in ligand binding, transcriptional ... ligandbinding domains, but each has its own isoform-specific segment in the N-terminal region of A/B domain which contains a transactivating domain [40] The predicted sequence of A/B domain of ... imaginal wing cells of P interpunctella at the last larval instar In the fifth larval instar of M sexta, it has been reported in vitro that 20E induced a coexpression of MsEcR-B1 and MsEcR-A during...

Ngày tải lên: 23/03/2014, 13:20

... cytokinins and hormones The main purpose of a regulated gene expression system is to control the timing and levels of transgene expression in vivo Whether the expressed protein remains within the ... regulated expression of the transgene finely tuned to the changing clinical state of a patient Several gene switches have been developed for regulating the expression of transgenes in humans [2] ... ligand induced changes in the expression of genes such as v-jun, FBJ and EGR 20-Hydroxyecdysone (20E) did not affect gene expression, but Pon A caused some changes in the expression of endogenous genes...

Ngày tải lên: 30/03/2014, 03:20

... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... level of expression for each gene (± standard error) compared to the house keeping gene GAPDH The increase in ADAMTS gene expression in the CrMFC approached significance and was included in the ... to match the surface area of the condyle The areas of India ink staining were outlined using a permanent marker Tracings of the India ink-stained tibial and femoral condyles were evaluated without...

Ngày tải lên: 20/06/2014, 00:20

... CINP NUFIP1 IQGAP2 nucleosome assembly protein 1-like destrin proline rich utrophin histidine-rich calcium-binding protein anillin GTP-binding protein, member RAS oncogene family alpha tubulin ... and binding Interestingly, five actin-binding genes appeared in the list; namely: anillin (ANLN, ID: R16712), destrin (DSTN, ID: AA424824), utrophin (UTRN, ID: AA676840), cyclindependent kinase ... hybridization The gene library for the present project was commercially obtained from Research Genetics (Invitrogen, CA), containing 7489 genes, including 7019 known genes, 249 unknown genes and 110...

Ngày tải lên: 20/06/2014, 01:20

... disparate leptin gene expression cannot be excluded A major finding of this study was the consistent enhanced ovarian expression of genes involved in cholesterol biosynthesis in obese LY mice Aging LY ... Figure pared (in bold type) Genesto black mice involved in cholesterol biosynthesis exhibiting elevated expression (p < 0.05) in 180-day LY mice as comGenes (in bold type) involved in cholesterol ... by hyperinsulinemia/insulin resistance [37] Moreover, leptin bi-phasically modulates granulosa cell Star expression [36] An interesting and unexpected finding was the reciprocal shift in Hsd11b1...

Ngày tải lên: 20/06/2014, 07:20

... entire ligninolytic system in white-rot fungi could be used as a target for breeding a high lignin-degrading strain and for furthering our understanding of the lignin-degradation system in these ... play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and ... Belinky PA (2010) Involvement of protein kinase C in lignin peroxidase expression in oxygenated cultures of the white rot fungus Phanerochaete chrysosporium Enzyme Microb Technol 47:59-63 Minami...

Ngày tải lên: 20/06/2014, 20:20

... two revealed 37 genes which were differentially expressed in both strains Of these 37 genes, 14 showed similar expression patterns The remaining genes were primarily involved in various signal ... associated with it were included in this categorization Discussion In the present study, the effects of internal low-dose irradiation by 131I were investigated in vivo Using gene expression microarray, ... Gius D: Distinct effects of ionizing radiation on in vivo murine kidney and brain normal tissue gene expression Clin Cancer Res 2006, 12:3823-3830 Uehara Y, Ito Y, Taki K, Nenoi M, Ichinohe K, Nakamura...

Ngày tải lên: 20/06/2014, 21:20

... play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and ... another Ca 2+ signaling factor, protein kinase C, was reported to demonstrate involvement in ROS signaling underlying LiP expression (Matityahu et al 2010) However, our results indicate cross-talk ... doi:10.1038/nbt967 Matityahu A, Hadar Y, Belinky PA (2010) Involvement of protein kinase C in lignin peroxidase expression in oxygenated cultures of the white rot fungus Phanerochaete chrysosporium Enzyme...

Ngày tải lên: 21/06/2014, 17:20

... play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and ... another Ca 2+ signaling factor, protein kinase C, was reported to demonstrate involvement in ROS signaling underlying LiP expression (Matityahu et al 2010) However, our results indicate cross-talk ... doi:10.1038/nbt967 Matityahu A, Hadar Y, Belinky PA (2010) Involvement of protein kinase C in lignin peroxidase expression in oxygenated cultures of the white rot fungus Phanerochaete chrysosporium Enzyme...

Ngày tải lên: 21/06/2014, 19:20